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bottom chamber contained serum free rpmi sdf 1 cxcl12  (R&D Systems)


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    R&D Systems bottom chamber contained serum free rpmi sdf 1 cxcl12
    Bottom Chamber Contained Serum Free Rpmi Sdf 1 Cxcl12, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cxcl12/Recombinant+Human+CXCL12%2FSDF-1+gamma+Protein/pmc13592517-593-1-12
    Average 93 stars, based on 50 article reviews
    bottom chamber contained serum free rpmi sdf 1 cxcl12 - by Bioz Stars, 2026-10
    93/100 stars

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    Migration:

    Article Title: Cytotoxic chemotherapy potentiates the immune response and efficacy of combination CXCR4/PD-1 inhibition in models of pancreatic ductal adenocarcinoma
    Article Snippet: .. Human cryopreserved PBMCs from patient with PDAC were thawed, washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). .. PBMC (50,000) suspension in 100μL was loaded in the upper wells of Boyden chamber plates (Corning Transwell Supplier no. 3422; 8-μm membrane pore size).

    Article Title: IgE-producing cells on the move: CCR2 is a key regulator of IgE + plasma cell migration
    Article Snippet: .. More specifically, we tested the migration of IgE + and IgG1 + cells in response to recombinant human CXCL12 (R&D systems; 300ng/mL), CCL2 (Biolegend; 10ng/mL, 100ng/mL and 300ng/mL) and CCL28 (R&D systems; 300ng/mL and 1.5ug/mL). ..

    Recombinant:

    Article Title: Cytotoxic chemotherapy potentiates the immune response and efficacy of combination CXCR4/PD-1 inhibition in models of pancreatic ductal adenocarcinoma
    Article Snippet: .. Human cryopreserved PBMCs from patient with PDAC were thawed, washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). .. PBMC (50,000) suspension in 100μL was loaded in the upper wells of Boyden chamber plates (Corning Transwell Supplier no. 3422; 8-μm membrane pore size).

    Article Title: IgE-producing cells on the move: CCR2 is a key regulator of IgE + plasma cell migration
    Article Snippet: .. More specifically, we tested the migration of IgE + and IgG1 + cells in response to recombinant human CXCL12 (R&D systems; 300ng/mL), CCL2 (Biolegend; 10ng/mL, 100ng/mL and 300ng/mL) and CCL28 (R&D systems; 300ng/mL and 1.5ug/mL). ..

    Article Title: CD142 Identifies Neoplastic Desmoid Tumor Cells, Uncovering Interactions Between Neoplastic and Stromal Cells That Drive Proliferation
    Article Snippet: .. All recombinant proteins were purchased from R&D Systems, reconstituted as per the manufacturer’s instructions and used at the following concentrations: Human CCL2 (R&D Systems, 279-MC-010, 100 ng/mL), human CHI3L1 (R&D Systems, 2599-CH-050, 100 ng/mL), human CXCL12 (R&D Systems, 350-NS010, 100 ng/mL), human IGFBP3 (R&D Systems, 675-B3-025, 100 ng/mL), human PTX3 (R&D Systems, 1826-TS-025, 1μg/mL), and mouseWnt3a (R&D Systems, 1324-WN-010, 25 ng/mL). ..

    Article Title: BACH2-mediated CD28 and CD40LG axes contribute to pathogenesis and progression of T-cell lymphoblastic leukemia
    Article Snippet: Cells were then added to transwell inserts with 8 μm pore size polycarbonate filters (Corning, Kennebunk, ME, USA) in a 24-well plate. .. Culture media containing the pre-established monolayer of HS-5 BMSCs or complete media with recombinant human CXCL12 (rhCXCL12, R&D Systems, 10 ng/mL) were placed in the lower wells. ..

    Article Title: Exploring the effect of canine cancer-associated fibroblasts on T cell dynamics through the CXCL12/CXCR4 axis modulated by TGF-β1
    Article Snippet: Canine CD8+ T cells with or without pretreatment with 4 μM AMD3100 (Abcam), a CXCR4 antagonist, were added to the upper compartment of the cell culture inserts (pore size, 8 μm; Corning Inc, Corning, NY, USA). .. The lower compartment was supplemented with either 100 ng/ml of recombinant human CXCL12 (R&D Systems) or CAFs-CM. ..

    Article Title: BACH2-mediated CD28 and CD40LG axes contribute to pathogenesis and progression of T-cell lymphoblastic leukemia.
    Article Snippet: Cells were then added to transwell inserts with 8 μm pore size polycarbonate filters (Corning, Kennebunk, ME, USA) in a 24-well plate. .. Culture media containing the pre-established monolayer of HS-5 BMSCs or complete media with recombinant human CXCL12 (rhCXCL12, R&D Systems, 10 ng/mL) were placed in the lower wells. ..



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    CAF‐derived CXCL12 reinforces PDIA6 expression through CXCR4‐STAT3 signaling in GC. (A,B) Immunofluorescence staining and western blot analysis of the CAF markers FAP and α‐SMA in CAFs and paired NFs. (C,D) qRT‐PCR and western blot analyses of PDIA6 mRNA and protein expression in GC cells treated with CAF‐conditioned medium (CAF‐CM) or NF‐conditioned medium (NF‐CM). (E,F) Transwell migration and EdU incorporation assays evaluating the effects of PDIA6 knockdown on CAF‐CM‐induced GC cell phenotypes. (G) Western blot analysis of STAT3 and PDIA6 in GC cells treated with or without CAF‐CM and transfected with control or STAT3 siRNA. (H) RNA‐seq analysis identifying CXCL12 as a highly upregulated secreted factor in CAFs relative to NFs. (I) Correlation analysis of CXCL12 and PDIA6 expression in the TCGA‐STAD cohort. (J,K) Single‐cell RNA‐seq analysis showing fibroblast‐specific expression of CXCL12 in GC tissues. (L,M) Western blot analysis of CXCL12 protein expression and ELISA of CXCL12 secretion in CAFs, NFs, and GC cells. (N) Western blot analysis of p‐STAT3 (Tyr705), total STAT3, and PDIA6 in GC cells pretreated with AMD3100 (2.5 µg/mL) for 30 min and then incubated with or without CAF‐CM for 24 h. (O) Western blot analysis of p‐STAT3 (Tyr705), total STAT3, and PDIA6 in GC cells treated with CAF‐CM together with IgG control or αCXCL12 (10 µg/mL, 24 h). (P) Western blot analysis of p‐STAT3 (Tyr705) and PDIA6 in GC cells treated with CAF‐CM or recombinant human CXCL12 (rhCXCL12, 25/50/100 ng/mL) for 24 h, with or without AMD3100 pretreatment. (Q,R) Cell proliferation and migration assays evaluating the effects of rhCXCL12 stimulation and αCXCL12 neutralization on GC cell behavior. Data are presented as mean ± SD.

    Journal: Advanced Science

    Article Title: PDIA6–SCD1 Axis Rewires Lipid Metabolism to Drive Gastric Cancer Progression

    doi: 10.1002/advs.75923

    Figure Lengend Snippet: CAF‐derived CXCL12 reinforces PDIA6 expression through CXCR4‐STAT3 signaling in GC. (A,B) Immunofluorescence staining and western blot analysis of the CAF markers FAP and α‐SMA in CAFs and paired NFs. (C,D) qRT‐PCR and western blot analyses of PDIA6 mRNA and protein expression in GC cells treated with CAF‐conditioned medium (CAF‐CM) or NF‐conditioned medium (NF‐CM). (E,F) Transwell migration and EdU incorporation assays evaluating the effects of PDIA6 knockdown on CAF‐CM‐induced GC cell phenotypes. (G) Western blot analysis of STAT3 and PDIA6 in GC cells treated with or without CAF‐CM and transfected with control or STAT3 siRNA. (H) RNA‐seq analysis identifying CXCL12 as a highly upregulated secreted factor in CAFs relative to NFs. (I) Correlation analysis of CXCL12 and PDIA6 expression in the TCGA‐STAD cohort. (J,K) Single‐cell RNA‐seq analysis showing fibroblast‐specific expression of CXCL12 in GC tissues. (L,M) Western blot analysis of CXCL12 protein expression and ELISA of CXCL12 secretion in CAFs, NFs, and GC cells. (N) Western blot analysis of p‐STAT3 (Tyr705), total STAT3, and PDIA6 in GC cells pretreated with AMD3100 (2.5 µg/mL) for 30 min and then incubated with or without CAF‐CM for 24 h. (O) Western blot analysis of p‐STAT3 (Tyr705), total STAT3, and PDIA6 in GC cells treated with CAF‐CM together with IgG control or αCXCL12 (10 µg/mL, 24 h). (P) Western blot analysis of p‐STAT3 (Tyr705) and PDIA6 in GC cells treated with CAF‐CM or recombinant human CXCL12 (rhCXCL12, 25/50/100 ng/mL) for 24 h, with or without AMD3100 pretreatment. (Q,R) Cell proliferation and migration assays evaluating the effects of rhCXCL12 stimulation and αCXCL12 neutralization on GC cell behavior. Data are presented as mean ± SD.

    Article Snippet: CXCL12 levels in cell culture supernatants were quantified using a human CXCL12 ELISA kit (RK00266, Abclonal, China) according to the manufacturer's instructions.

    Techniques: Derivative Assay, Expressing, Immunofluorescence, Staining, Western Blot, Quantitative RT-PCR, Migration, Knockdown, Transfection, Control, RNA Sequencing, Single Cell, Enzyme-linked Immunosorbent Assay, Incubation, Recombinant, Neutralization