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human cscc cell lines a431 cells  (ATCC)


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    ATCC human cscc cell lines a431 cells
    Human Cscc Cell Lines A431 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 3800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cscc+cell+lines/A-431/pm42277814-212-0-7
    Average 98 stars, based on 3800 article reviews
    human cscc cell lines a431 cells - by Bioz Stars, 2026-11
    98/100 stars

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    Cell Culture:

    Article Title: Hypoxia-induced ZEB1 promotes cervical cancer immune evasion by strengthening the CD47-SIRPα axis
    Article Snippet: .. Human CSCC cell lines (SiHa and C33a) and the human monocytic cell line THP-1 were purchased from the American Type Culture Collection (ATCC, USA) and cultured according to the provider’s guidelines. .. Hypoxia exposure was performed by placing cells in a hypoxic incubator (Thermo Fisher Scientific, USA) maintained at low oxygen tension (1% O 2 , 5% CO 2 , and 94% N 2 ).

    Article Title: LNMAC Promotes Cervical Squamous Cell Carcinoma Lymphatic Metastasis via Epigenetic Regulation of FGF2-Induced Lymphangiogenesis.
    Article Snippet: .. Cell Culture: Human CSCC cell lines (SiHa, SW756, MS751) were purchased from the American Type Culture Collection (ATCC, USA). .. Human lymphatic endothelial cells (HLECs) were obtained from ScienCell Research Laboratories and cultured in the ECM (ScienCell, CA).

    Article Title: From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses
    Article Snippet: .. Cell culture: Two human CSCC cell lines (A431 and SCL-1) and a human immortalized keratinocytes cell line Hacat (as normal control) were obtained from American Tissue Culture Collection (Manassas, VA, USA). .. The cells were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (Invitrogen) and 1% penicillin/streptomycin (Invitrogen) and incubated at 37°C in 5% carbon dioxide.

    Article Title: Hypoxia-induced ZEB1 promotes cervical cancer immune evasion by strengthening the CD47-SIRPα axis.
    Article Snippet: .. Human CSCC cell lines (SiHa and C33a) and the human monocytic cell line THP-1 were purchased from the American Type Culture Collection (ATCC, USA) and cultured according to the provider’s guidelines. .. Hypoxia exposure was performed by placing cells in a hypoxic incubator (Thermo Fisher Scientific, USA) maintained at low oxygen tension (1% O2, 5% CO2, and 94% N2).

    Article Title: From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses.
    Article Snippet: .. Cell culture: Two human CSCC cell lines (A431 and SCL-1) and a human immortalized keratinocytes cell line Hacat (as normal control) were obtained from American Tissue Culture Collection (Manassas, VA, USA). .. The cells were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (Invitrogen) and 1% penicillin/streptomycin (Invitrogen) and incubated at 37°C in 5% carbon dioxide.

    Control:

    Article Title: From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses
    Article Snippet: .. Cell culture: Two human CSCC cell lines (A431 and SCL-1) and a human immortalized keratinocytes cell line Hacat (as normal control) were obtained from American Tissue Culture Collection (Manassas, VA, USA). .. The cells were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (Invitrogen) and 1% penicillin/streptomycin (Invitrogen) and incubated at 37°C in 5% carbon dioxide.

    Article Title: From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses.
    Article Snippet: .. Cell culture: Two human CSCC cell lines (A431 and SCL-1) and a human immortalized keratinocytes cell line Hacat (as normal control) were obtained from American Tissue Culture Collection (Manassas, VA, USA). .. The cells were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (Invitrogen) and 1% penicillin/streptomycin (Invitrogen) and incubated at 37°C in 5% carbon dioxide.



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    human cscc cell lines  (ATCC)
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    Oncolytic activity of CVB3/2035A in human <t>CSCC</t> cell lines and cell line-derived xenografts. (A) CSCC cell monolayers were either mock-infected or infected with CVB3/2035A at an MOI of 1. Photomicrographs were taken at 72 h post-infection. Scale bar, 100 μm. (B) CSCC cells were infected with CVB3/2035A at an MOI of 10, 1, 0.1, and 0.01 for 72 h. Cell viability was assessed using the CCK8 assay. (C) Nude mice <t>bearing</t> <t>C33A,</t> SiHa, and CaSki xenografts were treated i.t. or i.v. with five consecutive doses of CVB3/2035A (1 × 10 8 TCID 50 per dose). An equivalent volume of PBS was administered i.t. as a control (Mock). Tumor growth was monitored over a 20-day period. (D) The combination of CVB3/2035A and paclitaxel improved efficacy in CSCC CDX models. Nude mice with C33A, SiHa, and CaSki xenografts were either mock-treated (PBS i.t. + PTX diluting solution i.p.) or treated with CVB3/2035A (1 × 10 8 TCID 50 i.t. + PTX diluting solution i.p.), PTX (5 mg/kg i.p. + PBS i.t.), or a combination (1 × 10 8 TCID 50 CVB3/2035A i.t. + 5 mg/kg PTX i.p.). Data are presented as means ± SEM. Statistical analysis was performed using a two-way ANOVA ( n = 5). Black arrows indicate treatments; ns, not significant; * p < 0.05; **** p < 0.0001
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    Oncolytic activity of CVB3/2035A in human CSCC cell lines and cell line-derived xenografts. (A) CSCC cell monolayers were either mock-infected or infected with CVB3/2035A at an MOI of 1. Photomicrographs were taken at 72 h post-infection. Scale bar, 100 μm. (B) CSCC cells were infected with CVB3/2035A at an MOI of 10, 1, 0.1, and 0.01 for 72 h. Cell viability was assessed using the CCK8 assay. (C) Nude mice bearing C33A, SiHa, and CaSki xenografts were treated i.t. or i.v. with five consecutive doses of CVB3/2035A (1 × 10 8 TCID 50 per dose). An equivalent volume of PBS was administered i.t. as a control (Mock). Tumor growth was monitored over a 20-day period. (D) The combination of CVB3/2035A and paclitaxel improved efficacy in CSCC CDX models. Nude mice with C33A, SiHa, and CaSki xenografts were either mock-treated (PBS i.t. + PTX diluting solution i.p.) or treated with CVB3/2035A (1 × 10 8 TCID 50 i.t. + PTX diluting solution i.p.), PTX (5 mg/kg i.p. + PBS i.t.), or a combination (1 × 10 8 TCID 50 CVB3/2035A i.t. + 5 mg/kg PTX i.p.). Data are presented as means ± SEM. Statistical analysis was performed using a two-way ANOVA ( n = 5). Black arrows indicate treatments; ns, not significant; * p < 0.05; **** p < 0.0001

    Journal: Virology Journal

    Article Title: Oncolytic activity of a coxsackievirus B3 strain in patient-derived cervical squamous cell carcinoma organoids and synergistic effect with paclitaxel

    doi: 10.1186/s12985-024-02502-y

    Figure Lengend Snippet: Oncolytic activity of CVB3/2035A in human CSCC cell lines and cell line-derived xenografts. (A) CSCC cell monolayers were either mock-infected or infected with CVB3/2035A at an MOI of 1. Photomicrographs were taken at 72 h post-infection. Scale bar, 100 μm. (B) CSCC cells were infected with CVB3/2035A at an MOI of 10, 1, 0.1, and 0.01 for 72 h. Cell viability was assessed using the CCK8 assay. (C) Nude mice bearing C33A, SiHa, and CaSki xenografts were treated i.t. or i.v. with five consecutive doses of CVB3/2035A (1 × 10 8 TCID 50 per dose). An equivalent volume of PBS was administered i.t. as a control (Mock). Tumor growth was monitored over a 20-day period. (D) The combination of CVB3/2035A and paclitaxel improved efficacy in CSCC CDX models. Nude mice with C33A, SiHa, and CaSki xenografts were either mock-treated (PBS i.t. + PTX diluting solution i.p.) or treated with CVB3/2035A (1 × 10 8 TCID 50 i.t. + PTX diluting solution i.p.), PTX (5 mg/kg i.p. + PBS i.t.), or a combination (1 × 10 8 TCID 50 CVB3/2035A i.t. + 5 mg/kg PTX i.p.). Data are presented as means ± SEM. Statistical analysis was performed using a two-way ANOVA ( n = 5). Black arrows indicate treatments; ns, not significant; * p < 0.05; **** p < 0.0001

    Article Snippet: Three human CSCC cell lines (C33A, SiHa, and CaSki) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Activity Assay, Derivative Assay, Infection, CCK-8 Assay, Control