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human serum  (Innovative Research Inc)


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    Innovative Research Inc human serum
    Human Serum, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+serum/Pooled+Human+Complement+Serum/us12558398-1207-1-3
    Average 93 stars, based on 49 article reviews
    human serum - by Bioz Stars, 2026-10
    93/100 stars

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    other:

    Article Title: Development of a Liposome-Based Serological Assay for SARS-CoV‑2 Variants with Special Emphasis on Coupling Chemistries Required to Maintain Protein Antigenicity
    Article Snippet: Pooled Human Complement Serum (IR45270 and IR46827) was obtained from Innovative Research (Novi, MI, USA).

    Incubation:

    Article Title: Complement-dependent cytotoxicity of human autoantibodies against myelin oligodendrocyte glycoprotein
    Article Snippet: .. Anti-MOG-positive sera were added to the MOG-expressing cell aliquots (mixture of each 1 × 10 5 cells of MOG-GFP and empty cells) at 20% v/v in DMEM and incubated at room temperature for 30 min, followed by sequential reaction with 20% v/v of Low-Tox-H rabbit complement (Cedarlane, Ontario) or pooled human complement serum (Innovative Research, MI, United States) at 37°C for 30 min. .. Cells were washed once, stained with propidium iodide (PI), and analyzed using a BD LSRFortessa flow cytometer (BD Biosciences, CA, United States).

    Article Title: Recombinant Ixodes scapularis Calreticulin Binds Complement Proteins but Does Not Protect Borrelia burgdorferi from Complement Killing
    Article Snippet: .. The test proteins, human complement serum (HCS; Innovative Research, Inc., Novi, MI, USA) were diluted to 10% in the pull-down buffer (6.5 mM sodium phosphate (pH 7.4), 140 mM NaCl, 0.02% Tween-20), added to the bead–bait complex, and incubated on a roller for 2 h at RT. ..

    Purification:

    Article Title: C3a Mediates Endothelial Barrier Disruption in Brain-Derived, but Not Retinal, Human Endothelial Cells
    Article Snippet: .. To obtain purified IgG for endothelial transbarrier assays, 2 mL pooled human complement serum (Innovative Research, Inc., Novi, MI, USA, #ICSER) were subjected to protein G spin column (Thermo Fisher Scientific, Waltham, MA, USA, #89957) purification. ..

    Article Title: C3a Mediates Endothelial Barrier Disruption in Brain-Derived, but Not Retinal, Human Endothelial Cells.
    Article Snippet: .. To obtain purified IgG for endothelial transbarrier assays, 2 mL pooled human complement serum (Innovative Research, Inc., Novi, MI, USA, #ICSER) were subjected to protein G spin column (Thermo Fisher Scientific, Waltham, MA, USA, #89957) purification. ..



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    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Journal: Science Advances

    Article Title: Xenopus IgX informs engineering strategies of IgM and IgG hexamers

    doi: 10.1126/sciadv.aea3737

    Figure Lengend Snippet: ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Article Snippet: The RTX-Fcμ or RTX-Fcγ proteins were incubated with equal volumes of cell cultures (~20,000 cells) and 12% normal human serum complement (Quidel) sequentially and then transferred into a 96-microwell plate.

    Techniques: Cell Culture