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human chemerin quantikine elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems human chemerin quantikine elisa kit
    Human Chemerin Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+chemerin/Human+Chemerin+Quantikine+ELISA/pm41947503-54-7-12
    Average 94 stars, based on 28 article reviews
    human chemerin quantikine elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway
    Article Snippet: .. Then, the cells were washed three times to remove cell debris, pretreated with or without PD98059 (MCE, New Jersey, United States) for 30 min and cultured with recombinant human chemerin (R&D, Minneapolis, United States) as indicated. .. Wound closure was observed and photographed at 0 and 12 h with a microscope (Leica Microsystems DFC550, Germany).

    Recombinant:

    Article Title: Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway
    Article Snippet: .. Then, the cells were washed three times to remove cell debris, pretreated with or without PD98059 (MCE, New Jersey, United States) for 30 min and cultured with recombinant human chemerin (R&D, Minneapolis, United States) as indicated. .. Wound closure was observed and photographed at 0 and 12 h with a microscope (Leica Microsystems DFC550, Germany).

    Article Title: Chemerin contributes to inflammatory responses and suppresses osteogenic differentiation in chronic periodontitis.
    Article Snippet: Chronic periodontitis (CP) is a longterm inflammation of the periodontal tissues, resulting from profuse amounts of dental plaques (Cardoso et al., 2018).. It is a common disease of oral cavity and one of the seven categories of periodontitis.. Periodontitis initially starts as gingivitis with low symptoms, such as bleeding gum and bad breath, and gradually progresses onto CP and eventually aggressive periodontitis (Smith et al., 2017).

    Article Title: Statins, but not proprotein convertase subtilisin‐kexin type 9 inhibitors, lower chemerin in hypercholesterolemia via low‐density lipoprotein receptor upregulation
    Article Snippet: .. To conduct the pull‐down assay, 300 μL of this plasma pool was incubated for 30 min at room temperature with either 1 μg anti‐human chemerin labeled with streptavidin (R&D Systems, Abingdon, UK) or 5 μg recombinant human chemerin‐His protein (Thermo Fisher Scientific). .. Subsequently, 50 μL of prewashed Dynabeads of streptavidin (Thermo Fisher Scientific) was added to the plasma pool with anti‐human chemerin labeled with streptavidin, and 50 μL of prewashed Dynabeads of His‐tag (Thermo Fisher Scientific) was added to the plasma pool with human chemerin‐His protein.

    Article Title: Chemerin promotes invasion of oral squamous cell carcinoma cancer by stimulating IL-6/TNF-α production via STAT3 signaling pathway
    Article Snippet: 2.2 Cell culture and treatment Human OSCC cell lines SCC25 and SCC9 were obtained from ATCC (ATCC, Manassas, VA, USA) and cultured in DMEM (Gibco, BRL, San Francisco, CA, USA) supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic-antimycotic and maintained at 37°C in a humidi ed Page 4/15 incubator containing 5% CO2. .. The SCC25 and SCC9 cells were treated with recombinant human chemerin (R-chemerin; 2324-CM-025, R&D Systems, USA) at various concentrations (10, 50, 100ng/ml) for 24h. .. For the inhibitor treatment, we treated SCC25 and SCC9 with STAT3 inhibitor, cryptotanshinone (10 μM, C5624, Sigma, USA) for 24 h. 2.3 Enzyme-linked immunosorbent assay (ELISA) The concentrations of IL-6, TNF-α, VEGF, IL15, GM-CSF, RANTES, and chemerin in the blood samples and culture medium were detected using an ELISA kit (R&D Systems) according to the manufacturer’s instructions.

    Article Title: Chemerin as a Novel Mediator of Fetal Endothelial Dysfunction in Gestational Diabetes Mellitus: Integration of Bioinformatics, Clinical, and In Vitro Evidence.
    Article Snippet: Objective: This study aimed to elucidate chemerin's role in gestational diabetes mellitus (GDM)related fetal endothelial dysfunction and its association with noninvasive hemodynamic parameters.. Methods: We integrated bioinformatics (GSE87295 dataset, n = 5/group), a prospective clinical cohort (30 GDM, 31 controls), and functional in vitro experiments.. Chemerin and von Willebrand factor (vWF) were quantified by ELISA.

    Article Title: Chemerin promotes invasion of oral squamous cell carcinoma by stimulating IL-6 and TNF-α production via STAT3 activation.
    Article Snippet: diagnosed at an early stage (I or II), but decreases to 40 ~ 50% for patients diagnosed at an advanced stage (III or IV) [4–7].. Cervical lymph node metastasis is an important prognostic factor for OSCC, with survival rates being reduced in cases with regional metastasis [8].. A deeper understanding of OSCC pathogenesis and identification of predictive biomarkers are needed to potentially improve OSCC survival.

    Article Title: Small molecule CMKLR1 antagonists in inflammatory disease
    Article Snippet: .. After 10 min, 5 ul of recombinant human chemerin (R&D Systems) at a final concentration of 20 nM was added to each well. ..

    Pull Down Assay:

    Article Title: Statins, but not proprotein convertase subtilisin‐kexin type 9 inhibitors, lower chemerin in hypercholesterolemia via low‐density lipoprotein receptor upregulation
    Article Snippet: .. To conduct the pull‐down assay, 300 μL of this plasma pool was incubated for 30 min at room temperature with either 1 μg anti‐human chemerin labeled with streptavidin (R&D Systems, Abingdon, UK) or 5 μg recombinant human chemerin‐His protein (Thermo Fisher Scientific). .. Subsequently, 50 μL of prewashed Dynabeads of streptavidin (Thermo Fisher Scientific) was added to the plasma pool with anti‐human chemerin labeled with streptavidin, and 50 μL of prewashed Dynabeads of His‐tag (Thermo Fisher Scientific) was added to the plasma pool with human chemerin‐His protein.

    Clinical Proteomics:

    Article Title: Statins, but not proprotein convertase subtilisin‐kexin type 9 inhibitors, lower chemerin in hypercholesterolemia via low‐density lipoprotein receptor upregulation
    Article Snippet: .. To conduct the pull‐down assay, 300 μL of this plasma pool was incubated for 30 min at room temperature with either 1 μg anti‐human chemerin labeled with streptavidin (R&D Systems, Abingdon, UK) or 5 μg recombinant human chemerin‐His protein (Thermo Fisher Scientific). .. Subsequently, 50 μL of prewashed Dynabeads of streptavidin (Thermo Fisher Scientific) was added to the plasma pool with anti‐human chemerin labeled with streptavidin, and 50 μL of prewashed Dynabeads of His‐tag (Thermo Fisher Scientific) was added to the plasma pool with human chemerin‐His protein.

    Incubation:

    Article Title: Statins, but not proprotein convertase subtilisin‐kexin type 9 inhibitors, lower chemerin in hypercholesterolemia via low‐density lipoprotein receptor upregulation
    Article Snippet: .. To conduct the pull‐down assay, 300 μL of this plasma pool was incubated for 30 min at room temperature with either 1 μg anti‐human chemerin labeled with streptavidin (R&D Systems, Abingdon, UK) or 5 μg recombinant human chemerin‐His protein (Thermo Fisher Scientific). .. Subsequently, 50 μL of prewashed Dynabeads of streptavidin (Thermo Fisher Scientific) was added to the plasma pool with anti‐human chemerin labeled with streptavidin, and 50 μL of prewashed Dynabeads of His‐tag (Thermo Fisher Scientific) was added to the plasma pool with human chemerin‐His protein.

    Labeling:

    Article Title: Statins, but not proprotein convertase subtilisin‐kexin type 9 inhibitors, lower chemerin in hypercholesterolemia via low‐density lipoprotein receptor upregulation
    Article Snippet: .. To conduct the pull‐down assay, 300 μL of this plasma pool was incubated for 30 min at room temperature with either 1 μg anti‐human chemerin labeled with streptavidin (R&D Systems, Abingdon, UK) or 5 μg recombinant human chemerin‐His protein (Thermo Fisher Scientific). .. Subsequently, 50 μL of prewashed Dynabeads of streptavidin (Thermo Fisher Scientific) was added to the plasma pool with anti‐human chemerin labeled with streptavidin, and 50 μL of prewashed Dynabeads of His‐tag (Thermo Fisher Scientific) was added to the plasma pool with human chemerin‐His protein.

    Concentration Assay:

    Article Title: Small molecule CMKLR1 antagonists in inflammatory disease
    Article Snippet: .. After 10 min, 5 ul of recombinant human chemerin (R&D Systems) at a final concentration of 20 nM was added to each well. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Urinary chemerin as a potential biomarker for inflammatory bowel disease
    Article Snippet: .. ELISA to measure human chemerin was from R&D Systems (Wiesbaden, Nordenstadt, Germany; Cat # DY2324). ..



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    R&D Systems human chemerin quantikine elisa
    Comparison between <t>chemerin</t> level in healthy (CTRL) patient’s serum, and psoriatic patient’s serum [pg/ml]. a- p < 0.05.
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    Image Search Results


    Serum omentin (A) and chemerin (B) concentrations in COVID-19 patients and controls. Bars represent medians with interquartile ranges (IQR); dots indicate individual data points. Between-group comparisons (COVID-19 vs. controls) were performed using the Mann–Whitney U test; within-patient day 0 vs. day 7 comparisons were performed using the Wilcoxon signed-rank test. p -values for control vs. day 0, control vs. day 7, and day 0 vs. day 7 comparisons are shown in each panel.

    Journal: Frontiers in Medicine

    Article Title: Serum omentin and chemerin levels in patients with coronavirus disease 2019

    doi: 10.3389/fmed.2026.1785101

    Figure Lengend Snippet: Serum omentin (A) and chemerin (B) concentrations in COVID-19 patients and controls. Bars represent medians with interquartile ranges (IQR); dots indicate individual data points. Between-group comparisons (COVID-19 vs. controls) were performed using the Mann–Whitney U test; within-patient day 0 vs. day 7 comparisons were performed using the Wilcoxon signed-rank test. p -values for control vs. day 0, control vs. day 7, and day 0 vs. day 7 comparisons are shown in each panel.

    Article Snippet: Serum concentrations of chemerin and omentin-1 were measured in duplicate using sandwich enzyme-linked immunosorbent assay (ELISA) kits (BioVendor, Brno, Czech Republic) (Chemerin Human ELISA, Cat. No. RD191136200R; Omentin-1 Human ELISA, Cat. No. RD191100200R) according to the manufacturer’s instructions.

    Techniques: MANN-WHITNEY, Control

    Comparison between chemerin level in healthy (CTRL) patient’s serum, and psoriatic patient’s serum [pg/ml]. a- p < 0.05.

    Journal: Frontiers in Medicine

    Article Title: Evaluation of chemerin levels in the pathogenesis of psoriasis

    doi: 10.3389/fmed.2026.1748469

    Figure Lengend Snippet: Comparison between chemerin level in healthy (CTRL) patient’s serum, and psoriatic patient’s serum [pg/ml]. a- p < 0.05.

    Article Snippet: Serum chemerin levels (pg/mL) were measured using the Human Chemerin Quantikine ELISA (R&D Systems) following the manufacturer’s instructions in the Department of Neurodegeneration Diagnostics.

    Techniques: Comparison

    The scatterplot shows a correlation between chemerin and the level of platelet count (PLT) in the serum of patients with psoriasis.

    Journal: Frontiers in Medicine

    Article Title: Evaluation of chemerin levels in the pathogenesis of psoriasis

    doi: 10.3389/fmed.2026.1748469

    Figure Lengend Snippet: The scatterplot shows a correlation between chemerin and the level of platelet count (PLT) in the serum of patients with psoriasis.

    Article Snippet: Serum chemerin levels (pg/mL) were measured using the Human Chemerin Quantikine ELISA (R&D Systems) following the manufacturer’s instructions in the Department of Neurodegeneration Diagnostics.

    Techniques: