hela human cervix adenocarcinoma cells  (ATCC)

Journal: Marine Drugs

Article Title: Receptor Binding, Functional Activity, and Cell Viability Assessment of Novel Marine-Based Hybrid Peptides from Raja porosa

doi: 10.3390/md24050181

Figure Lengend Snippet: In vitro anticancer effect of PK01# ( A , C ) and PK02# ( B , D ) on HeLa, SAS, PANC-1, and A-172 cancer cell lines after a 24 h incubation. Cell viability was evaluated by MTT assay. Statistical analysis was performed with GraphPad Prism 9.0. All data are presented as mean ± S.E.M. Two-way ANOVA followed by Dunnett’s post hoc test revealed significant changes in the SAS, HeLa, PANC-1, and A-172 cells (* p < 0.05, ** p < 0.01, *** p < 0.001), as compared to those of control fibroblasts. IC 50 was not determined (maximum inhibition < 50% in MTT assay).

Article Snippet: The cellosaurus cell line SAS, human glioblastoma (A-172), pancreatic ductal adenocarcinoma (PANC-1), and HeLa human cervix adenocarcinoma cells were purchased from ATCC (Manassas, VA, USA).

Techniques: In Vitro, Incubation, MTT Assay, Control, Inhibition

Journal: Marine Drugs

Article Title: Receptor Binding, Functional Activity, and Cell Viability Assessment of Novel Marine-Based Hybrid Peptides from Raja porosa

doi: 10.3390/md24050181

Figure Lengend Snippet: Comparison of cytotoxic effects of PK01# and PK02# after a 24 h incubation against ( A ) HeLa, ( B ) SAS, ( C ) PANC-1, and ( D ) A-172 cancer cell lines. Two-way ANOVA followed by Bonferroni’s post hoc test revealed a significant difference (* p < 0.05, ** p < 0.01) between PK01# and PK02# in SAS and A-172 cancer cells. UT denotes untreated cancer cells.

Article Snippet: The cellosaurus cell line SAS, human glioblastoma (A-172), pancreatic ductal adenocarcinoma (PANC-1), and HeLa human cervix adenocarcinoma cells were purchased from ATCC (Manassas, VA, USA).

Techniques: Comparison, Incubation

Journal: Cell Reports Methods

Article Title: Expanded applications of bioluminescence microscopy with phasor analysis

doi: 10.1016/j.crmeth.2026.101344

Figure Lengend Snippet: Bioluminescent phasor signatures capture distinct structural and spectral features (A) Table of reporters used in this study. Distinct luciferase fusions exhibit different BRET efficiencies and unique phasor outputs (represented by S , G coordinates) in the presence of furimazine (Fz). (B and C) Bioluminescent phasor signatures of Antares (B) and Antares2 (C). HeLa cells were transiently transfected with plasmids encoding the reporters. The cells were then treated with Fz (20 μM) ∼36 h post transfection. Images were acquired using the bioluminescent phasor microscope with a 20× air objective and 10 s/frame integration time. A total of 20 frames were collected for each sample and the phasor locations were computed. Each pixel in the resulting image was color-coded according to the phasor signature. Scale bars, 25 μm. (D) The phasor signatures of Antares and Antares2 are distinct from those of other red-shifted BRET reporters. (E) Compiled phasor signatures of common BRET reporters. The size of each circle represents the calculated BRET ratio.

Article Snippet: Human: HeLa (uterus/cervix) , ATCC , CCL-2 TM.

Techniques: Luciferase, Transfection, Microscopy

Journal: Cell Reports Methods

Article Title: Expanded applications of bioluminescence microscopy with phasor analysis

doi: 10.1016/j.crmeth.2026.101344

Figure Lengend Snippet: Bioluminescent phasor imaging through depth (A) Bioluminescent phasor images (left) and analysis plots (right) for YeNL-expressing HeLa cells embedded in a collagen matrix (2.0 mg/mL). Media containing furimazine (Fz, 50 μM) was added to the top of the sample, and light emission was recorded with the bioluminescent phasor microscope at various distances from the bottom of the slide. “Counts” represent the intensity above background. A total of 20 frames were collected per slice, and the phasor location was computed. Scale bars, 100 μm. (B) Excised subcutaneous tumors comprising UM-HMC-1 cells were analyzed. The cells expressed either CeNLuc, YeNL, or LumiScarlet, and mixtures of these reporter cells were also included. Spectra were measured on a luminometer (left), and phasor signatures were acquired with bioluminescent phasor (right). (C) LumiScarlet-expressing MDA-MB-231 cells implanted in a mouse were imaged using an EM-CCD camera (left), followed by the bioluminescent phasor microscope (right).

Article Snippet: Human: HeLa (uterus/cervix) , ATCC , CCL-2 TM.

Techniques: Imaging, Expressing, Microscopy

Journal: Cell Reports Methods

Article Title: Expanded applications of bioluminescence microscopy with phasor analysis

doi: 10.1016/j.crmeth.2026.101344

Figure Lengend Snippet: Caspase biosensing through depth (A) Caspase-9-responsive BRET (C9-BRET) sensor for reporting on cellular caspase-9 activity. Cleavage of the sensor results in a green-to-blue shift in emission. (B) Emission spectra of HeLa cells expressing C9-BRET in the presence and absence of staurosporine (STS, 5 μM). (C) Cleavage of C9-BRET monitored over time in the presence of STS (5 μM). The ratio of green (518 nm) to blue (460 nm) is shown, with p < 0.15 at 5 h and p < 0.001 at 7 h. p values (95% confidence interval) were calculated using Tukey’s multiple comparisons test. Data are presented as the mean ± SD for n = 3 replicates. (D) Schematic of C9-BRET biosensing via bioluminescent phasor analysis. (E) HeLa cells expressing C9-BRET embedded in a collagen matrix (2.0 mg/mL). Media containing furimazine (Fz, 50 μM) and STS (50 μM) was added to the top of the sample, and light emission was recorded over time and at various distances and imaged from the bottom (0 mm) and middle (0.5 mm), to top (1 mm) of the slide. A total of 20 frames were collected. BRET efficiency (eff.) represents the change in color (ratio of green: blue) over time. Values were scaled from 0 to 1.0. Representative phasor locations from the 0.5 mm (middle) plane at different time points were computed and are shown above the images. Scale bars, 100 μm.

Article Snippet: Human: HeLa (uterus/cervix) , ATCC , CCL-2 TM.

Techniques: Activity Assay, Expressing

Journal: Cell Reports Methods

Article Title: Expanded applications of bioluminescence microscopy with phasor analysis

doi: 10.1016/j.crmeth.2026.101344

Figure Lengend Snippet: BRET efficiency phasor detection using calcium biosensing (A) CaFluxVTN sensor for reporting on cellular Ca 2+ levels. Ca 2+ binding to troponin C (TnC) induces a conformational change, bringing NLuc in proximity to Venus. In the presence of the furimazine (Fz) and Ca 2+ , more yellow light is produced. (B) Spectra of HeLa cells expressing CaFluxVTN sensor in the presence (+) of Ca 2+ (500 μM) or no additional (−) Ca 2+ (endogenous levels). (C) Schematic of BRET trajectory and dynamic range of CaFluxVTN sensor on bioluminescent phasor. (D) Phasor signatures of HeLa cells expressing CaFluxVTN in the presence of EGTA (10 μM) (yellow highlight) or Ca 2+ (500 μM) (blue highlight). The positions of the donor and acceptor (in isolation) are shown for reference. A total of 20 frames were collected per slice, and the phasor locations were computed. (E) Single-cell BRET analyses using G and S coordinates derived from the corresponding phasor plots. BRET efficiency values were scaled from 0 to 1.0. Scale bars, 25 μm. (F) BRET efficiency measurements from single cells in (E). p < 0.0001. P (95% confidence interval) values were calculated using a two-sided t test. Data are presented as the mean ± SD for n = 10 cells.

Article Snippet: Human: HeLa (uterus/cervix) , ATCC , CCL-2 TM.

Techniques: Binding Assay, Produced, Expressing, Isolation, Single Cell, Derivative Assay

Journal: Cell Reports Methods

Article Title: Expanded applications of bioluminescence microscopy with phasor analysis

doi: 10.1016/j.crmeth.2026.101344

Figure Lengend Snippet: Multiplexed calcium biosensing in a mixed cell population (A) Spectra of HEK cells expressing Nano-Lantern(Ca 2+ ) sensor (Ca 2+ reporter 1 in green) and HeLa cells expressing CaFluxVTN (Ca 2+ reporter 2 in yellow) in the presence of furimazine (Fz) or coelenterazine (CTZ, 20 μM) (B) Phasor signatures of both reporters in isolation (Ca 2+ reporter 1 green cursor) (Ca 2+ reporter 2 yellow cursor) in the presence of Fz or CTZ (20 μM). (C) Phasor signatures of the mixture of the cells expressing each probe in (B) in the presence of Fz and CTZ (20 μM). (D) Images of the sensor-expressing HEK (smaller) and HeLa (larger) cells from (A), either spread out or in contact. Cells were treated with Fz and CTZ (20 μM). Pseudo color represents the intensity and phase (in radians). The phasor plot displays the locations of individual cells based on their phase and modulation characteristics. Scale bars, 25 μm. A total of 20 frames were collected per slice, and the phasor locations were computed. Data are representative of n = 3 replicate studies.

Article Snippet: Human: HeLa (uterus/cervix) , ATCC , CCL-2 TM.

Techniques: Expressing, Isolation

Journal: ACS Applied Materials & Interfaces

Article Title: Silver Nitrate and Bioactive Glass Containing Chitosan Coatings: Comparison of Antimicrobial and Antiviral Properties

doi: 10.1021/acsami.6c00215

Figure Lengend Snippet: Metallic air filters coated with chitosan/BG or chitosan/AgNO 3 showed no cytotoxicity on any tested cell lines. Cytotoxicity assessment was performed on (a) MRC-5 for HCoV-OC43, (b) HeLa 24 h for HRV-A1, (c) HeLa 96 h for AdV-5, and (d) MDCK for IFV-A H3N2. The cytotoxic effect of coatings on host cells was analyzed via optical microscope observation as reported in ISO guideline (a–d). Magnification, 400×. DMEM, culture medium; DMEM 2%, culture medium supplemented with 2% of serum; pure, pure wash-out solution; 1:10, 1:10 diluted wash-out solution.

Article Snippet: Human lung fibroblasts (MRC-5) (ATCC CCL-171), human cervix adenocarcinoma cells (HeLa) (ATCC CCL-2), and Madine-Darby canine kidney epithelial cells (MDCK) (ATCC CCL-34) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Sigma-Aldrich) supplemented with heat-inactivated, 10% (v/v) fetal bovine serum (FBS; Sigma-Aldrich) in a humidified 5% CO 2 incubator.

Techniques: Microscopy