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recombinant protein cd55 2009 cd  (R&D Systems)


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    Structured Review

    R&D Systems recombinant protein cd55 2009 cd
    Recombinant Protein Cd55 2009 Cd, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd55/Recombinant+Human+CD55%2FDAF+Protein/pmc12847889-337-1-8
    Average 93 stars, based on 8 article reviews
    recombinant protein cd55 2009 cd - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Development of a radionuclide-labeled monoclonal anti-CD55 antibody with theranostic potential in pleural metastatic lung cancer
    Article Snippet: A naïve chicken phage-displayed scFv library (constructed by SG Medical, Inc. as described previously , ) was used for bio-panning to select CD55-specific scFvs. .. Briefly, 5 × 10 6 magnetic beads (Dynabeads M-270 epoxy; Invitrogen) were coated with 2.5 μg of recombinant human CD55 (2009-CD/CF; R&D Systems) for each round of bio-panning (four rounds). ..

    Recombinant:

    Article Title: Development of a radionuclide-labeled monoclonal anti-CD55 antibody with theranostic potential in pleural metastatic lung cancer
    Article Snippet: A naïve chicken phage-displayed scFv library (constructed by SG Medical, Inc. as described previously , ) was used for bio-panning to select CD55-specific scFvs. .. Briefly, 5 × 10 6 magnetic beads (Dynabeads M-270 epoxy; Invitrogen) were coated with 2.5 μg of recombinant human CD55 (2009-CD/CF; R&D Systems) for each round of bio-panning (four rounds). ..

    Article Title: The expression profile and tumorigenic mechanisms of CD97 (ADGRE5) in glioblastoma render it a targetable vulnerability
    Article Snippet: .. Wells were then additionally coated with recombinant human CD55 (Cat# 2009-CD-050, R&D) or recombinant human THY1/CD90 (Cat# 16897-HCCH, Sino Biological) at a concentration of 24 μg/mL overnight. ..

    Article Title: SAR443809: a selective inhibitor of the complement alternative pathway, targeting complement factor Bb
    Article Snippet: .. Sequential injections of recombinant human CD55 (R&D Systems, Minneapolis, MN) and overnight buffer flow ensured all convertases decayed and only C3b remained on the chip. ..

    Article Title: The expression profile and tumorigenic mechanisms of CD97 (ADGRE5) in glioblastoma render it a targetable vulnerability
    Article Snippet: .. Wells were then additionally coated with recombinant human CD55 (Cat# 2009-CD-050, R&D) or recombinant human THY1/CD90 (Cat# 16897-HCCH, Sino Biological) at a concentration of 24 μg/mL overnight. ..

    Concentration Assay:

    Article Title: The expression profile and tumorigenic mechanisms of CD97 (ADGRE5) in glioblastoma render it a targetable vulnerability
    Article Snippet: .. Wells were then additionally coated with recombinant human CD55 (Cat# 2009-CD-050, R&D) or recombinant human THY1/CD90 (Cat# 16897-HCCH, Sino Biological) at a concentration of 24 μg/mL overnight. ..

    Article Title: The expression profile and tumorigenic mechanisms of CD97 (ADGRE5) in glioblastoma render it a targetable vulnerability
    Article Snippet: .. Wells were then additionally coated with recombinant human CD55 (Cat# 2009-CD-050, R&D) or recombinant human THY1/CD90 (Cat# 16897-HCCH, Sino Biological) at a concentration of 24 μg/mL overnight. ..



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    C′ regulators are not significantly upregulated in H1975 and HEp2 PI cells. ( A , B ) RNA samples from H1975 ( A ) and HEp2 ( B ) cells were collected as described in the legend to . Samples were analyzed via RT-qPCR to determine gene expression of three C′ regulators: CD46, <t>CD55,</t> and CFH. Values were normalized to expression of β-actin. C′ inhibitor expression in mock-infected cell cultures was set equal to 1 and expression in AI and PI cel cultures is expressed as fold change relative to mock. ( C ) Mock-infected, AI, and PI HEp2 cells were stained with an anti-CD55 antibody and processed via flow cytometry. MFI; Mean Fluorescence Intensity. ns stands for not significant, **** indicates p -value < 0.0001.
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    C′ regulators are not significantly upregulated in H1975 and HEp2 PI cells. ( A , B ) RNA samples from H1975 ( A ) and HEp2 ( B ) cells were collected as described in the legend to . Samples were analyzed via RT-qPCR to determine gene expression of three C′ regulators: CD46, <t>CD55,</t> and CFH. Values were normalized to expression of β-actin. C′ inhibitor expression in mock-infected cell cultures was set equal to 1 and expression in AI and PI cel cultures is expressed as fold change relative to mock. ( C ) Mock-infected, AI, and PI HEp2 cells were stained with an anti-CD55 antibody and processed via flow cytometry. MFI; Mean Fluorescence Intensity. ns stands for not significant, **** indicates p -value < 0.0001.
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    Image Search Results


    Expression of CD55 mRNA and protein in normal thymus, thymoma, and TSCC. The expression levels of CD55 mRNA ( A ) and protein ( B ) were significantly elevated progressively in normal thymus tissue, thymoma, and TSCC

    Journal: World Journal of Surgical Oncology

    Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

    doi: 10.1186/s12957-025-04051-2

    Figure Lengend Snippet: Expression of CD55 mRNA and protein in normal thymus, thymoma, and TSCC. The expression levels of CD55 mRNA ( A ) and protein ( B ) were significantly elevated progressively in normal thymus tissue, thymoma, and TSCC

    Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

    Techniques: Expressing

    Intensities of CD55 expression in TETs (IHC) scored 0: negative expression ( A , 40×; a, 200×); scored 1: low expression ( B , 40×; b, 200×); scored 2: moderate expression ( C , 40×; c, 200×); scored 3: high expression ( D , 40×; d, 200×)

    Journal: World Journal of Surgical Oncology

    Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

    doi: 10.1186/s12957-025-04051-2

    Figure Lengend Snippet: Intensities of CD55 expression in TETs (IHC) scored 0: negative expression ( A , 40×; a, 200×); scored 1: low expression ( B , 40×; b, 200×); scored 2: moderate expression ( C , 40×; c, 200×); scored 3: high expression ( D , 40×; d, 200×)

    Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

    Techniques: Expressing

    Proportion of CD55 expression in TETs (IHC) scored 0: negative expression ( A , 40×; a, 200×); scored 1: 1% − 30% positive cells ( B , 40×; b, 200×); scored 2: 30% − 60% positive cells ( C , 40×; c, 200×); scored 3: ≥ 60% positive cells ( D , 40×; d, 200×)

    Journal: World Journal of Surgical Oncology

    Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

    doi: 10.1186/s12957-025-04051-2

    Figure Lengend Snippet: Proportion of CD55 expression in TETs (IHC) scored 0: negative expression ( A , 40×; a, 200×); scored 1: 1% − 30% positive cells ( B , 40×; b, 200×); scored 2: 30% − 60% positive cells ( C , 40×; c, 200×); scored 3: ≥ 60% positive cells ( D , 40×; d, 200×)

    Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

    Techniques: Expressing

    Kaplan-Meier Survival Analysis in TETs The results demonstrated that PFS of TETs was closely related to CD55 mRNA ( A , p < 0.0001), CD55 protein ( B , p < 0.0001), tumor completeness of resection ( C , p < 0.0001), histological type ( D , p < 0.0001), Masaoka-Koga stage ( E , p < 0.0001), and adjuvant therapy ( F , p = 0.0009), but no with gender ( G , p = 0.1805) or MG ( H , p = 0.7269)

    Journal: World Journal of Surgical Oncology

    Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

    doi: 10.1186/s12957-025-04051-2

    Figure Lengend Snippet: Kaplan-Meier Survival Analysis in TETs The results demonstrated that PFS of TETs was closely related to CD55 mRNA ( A , p < 0.0001), CD55 protein ( B , p < 0.0001), tumor completeness of resection ( C , p < 0.0001), histological type ( D , p < 0.0001), Masaoka-Koga stage ( E , p < 0.0001), and adjuvant therapy ( F , p = 0.0009), but no with gender ( G , p = 0.1805) or MG ( H , p = 0.7269)

    Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

    Techniques: Adjuvant

    The relationship between CD55 and the clinical pathological features of patients The expression levels of CD55 mRNA and protein were higher in R1/R2 group ( A ), higher histological grade ( B ), Masaoka-Koga stage III/IV ( C ), adjuvant therapy ( D ) compared with the corresponding R0 group, normal thymus tissues, and stage I/II, and no-adjuvant therapy. Moreover, the expression of CD55 protein was significantly higher expressed in ≥ 60 years compared with ˂60 years ( E ). Additionally, CD55 protein expression was higher in males than that in females ( F ), whereas CD55 mRNA did not show similar results ( E, F ). Patients without MG showed higher CD55 mRNA and protein expression levels than those with MG, however, this difference was not statistically significant ( G ). There was also no statistically significant relationship between tumor diameter and CD55 expression ( H ). When CD55 protein was highly expressed, the mRNA level was also high ( I ).

    Journal: World Journal of Surgical Oncology

    Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

    doi: 10.1186/s12957-025-04051-2

    Figure Lengend Snippet: The relationship between CD55 and the clinical pathological features of patients The expression levels of CD55 mRNA and protein were higher in R1/R2 group ( A ), higher histological grade ( B ), Masaoka-Koga stage III/IV ( C ), adjuvant therapy ( D ) compared with the corresponding R0 group, normal thymus tissues, and stage I/II, and no-adjuvant therapy. Moreover, the expression of CD55 protein was significantly higher expressed in ≥ 60 years compared with ˂60 years ( E ). Additionally, CD55 protein expression was higher in males than that in females ( F ), whereas CD55 mRNA did not show similar results ( E, F ). Patients without MG showed higher CD55 mRNA and protein expression levels than those with MG, however, this difference was not statistically significant ( G ). There was also no statistically significant relationship between tumor diameter and CD55 expression ( H ). When CD55 protein was highly expressed, the mRNA level was also high ( I ).

    Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

    Techniques: Expressing, Adjuvant

    Kaplan-Meier Survival Analysis in high-risk TETs The results demonstrated that PFS of high-risk TETs was closely related to high expression of CD55 mRNA (A, p < 0.0001) and CD55 protein (B, p = 0.0168)

    Journal: World Journal of Surgical Oncology

    Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

    doi: 10.1186/s12957-025-04051-2

    Figure Lengend Snippet: Kaplan-Meier Survival Analysis in high-risk TETs The results demonstrated that PFS of high-risk TETs was closely related to high expression of CD55 mRNA (A, p < 0.0001) and CD55 protein (B, p = 0.0168)

    Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

    Techniques: Expressing

    C′ regulators are not significantly upregulated in H1975 and HEp2 PI cells. ( A , B ) RNA samples from H1975 ( A ) and HEp2 ( B ) cells were collected as described in the legend to . Samples were analyzed via RT-qPCR to determine gene expression of three C′ regulators: CD46, CD55, and CFH. Values were normalized to expression of β-actin. C′ inhibitor expression in mock-infected cell cultures was set equal to 1 and expression in AI and PI cel cultures is expressed as fold change relative to mock. ( C ) Mock-infected, AI, and PI HEp2 cells were stained with an anti-CD55 antibody and processed via flow cytometry. MFI; Mean Fluorescence Intensity. ns stands for not significant, **** indicates p -value < 0.0001.

    Journal: Pathogens

    Article Title: Relationship Between Cell Surface Viral Glycoprotein Expression and Resistance of Parainfluenza Virus Persistently Infected Cells to Complement-Mediated Lysis

    doi: 10.3390/pathogens14080815

    Figure Lengend Snippet: C′ regulators are not significantly upregulated in H1975 and HEp2 PI cells. ( A , B ) RNA samples from H1975 ( A ) and HEp2 ( B ) cells were collected as described in the legend to . Samples were analyzed via RT-qPCR to determine gene expression of three C′ regulators: CD46, CD55, and CFH. Values were normalized to expression of β-actin. C′ inhibitor expression in mock-infected cell cultures was set equal to 1 and expression in AI and PI cel cultures is expressed as fold change relative to mock. ( C ) Mock-infected, AI, and PI HEp2 cells were stained with an anti-CD55 antibody and processed via flow cytometry. MFI; Mean Fluorescence Intensity. ns stands for not significant, **** indicates p -value < 0.0001.

    Article Snippet: CD55 surface expression was monitored using an AF405 conjugated anti-human CD55 antibody (R&D Systems, FAB20091V).

    Techniques: Quantitative RT-PCR, Gene Expression, Expressing, Infection, Staining, Flow Cytometry, Fluorescence

    C′ regulators are not significantly upregulated in H1975 and HEp2 PI cells. ( A , B ) RNA samples from H1975 ( A ) and HEp2 ( B ) cells were collected as described in the legend to . Samples were analyzed via RT-qPCR to determine gene expression of three C′ regulators: CD46, CD55, and CFH. Values were normalized to expression of β-actin. C′ inhibitor expression in mock-infected cell cultures was set equal to 1 and expression in AI and PI cel cultures is expressed as fold change relative to mock. ( C ) Mock-infected, AI, and PI HEp2 cells were stained with an anti-CD55 antibody and processed via flow cytometry. MFI; Mean Fluorescence Intensity. ns stands for not significant, **** indicates p -value < 0.0001.

    Journal: Pathogens

    Article Title: Relationship Between Cell Surface Viral Glycoprotein Expression and Resistance of Parainfluenza Virus Persistently Infected Cells to Complement-Mediated Lysis

    doi: 10.3390/pathogens14080815

    Figure Lengend Snippet: C′ regulators are not significantly upregulated in H1975 and HEp2 PI cells. ( A , B ) RNA samples from H1975 ( A ) and HEp2 ( B ) cells were collected as described in the legend to . Samples were analyzed via RT-qPCR to determine gene expression of three C′ regulators: CD46, CD55, and CFH. Values were normalized to expression of β-actin. C′ inhibitor expression in mock-infected cell cultures was set equal to 1 and expression in AI and PI cel cultures is expressed as fold change relative to mock. ( C ) Mock-infected, AI, and PI HEp2 cells were stained with an anti-CD55 antibody and processed via flow cytometry. MFI; Mean Fluorescence Intensity. ns stands for not significant, **** indicates p -value < 0.0001.

    Article Snippet: CD55 surface expression was monitored using an AF405 conjugated anti-human CD55 antibody (R&D Systems, FAB20091V).

    Techniques: Quantitative RT-PCR, Gene Expression, Expressing, Infection, Staining, Flow Cytometry, Fluorescence