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anti human cd45 microbeads  (Miltenyi Biotec)


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    Miltenyi Biotec anti human cd45 microbeads
    Anti Human Cd45 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 469 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd45/CD45+MicroBeads%2C+human/bio_rxiv__64898__2026__10__07__757279-184-13-17
    Average 96 stars, based on 469 article reviews
    anti human cd45 microbeads - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Multiplex base editing of BCL11A regulatory elements to treat sickle cell disease
    Article Snippet: .. Cells were harvested from bone marrow (BM), thymus, spleen, and blood, and stained with antibodies against the following murine and human surface markers: murine CD45 (1/50 mCD45-VioBlue; Miltenyi Biotec), human CD45 (1/50 hCD45-APCvio770; Miltenyi Biotec). .. BM cells were also stained with human CD3 (1/50 CD3-APC; Miltenyi Biotec), human CD14 (1/50 CD14- PECy7; BD Biosciences), human CD15 (1/50 CD15-PE; Miltenyi Biotec), human CD11b (1/100 CD11b-APC; Miltenyi Biotec), human CD19 (1/100 CD19-BV510; BD Biosciences), human CD235a (1/50 CD235a-PE; BD Biosciences), human CD71 (1/10 CD71-APC; BD Biosciences), CD36 (1/50 CD36-FITC; BD Bio- sciences), and CD34 (1/100 CD34-PE-Vio770; Miltenyi Biotec).

    Article Title: Multiplex base editing of BCL11A regulatory elements to treat sickle cell disease
    Article Snippet: .. Cells were harvested from BM, thymus, spleen, and blood, and stained with antibodies against the following murine and human surface markers: murine CD45 (1/50 mCD45-VioBlue; Miltenyi Biotec), human CD45 (1/50 hCD45-APCvio770; Miltenyi Biotec). .. BM cells were also stained with human CD3 (1/50 CD3-APC; Miltenyi Biotec), human CD14 (1/50 CD14-PECy7; BD Biosciences), human CD15 (1/50 CD15-PE; Miltenyi Biotec), human CD11b (1/100 CD11b-APC; Miltenyi Biotec), human CD19 (1/100 CD19-BV510; BD Biosciences), human CD235a (1/50 CD235a-PE; BD Biosciences), human CD71 (1/10 CD71-APC; BD Biosciences), CD36 (1/50 CD36-FITC; BD Bio-sciences), and CD34 (1/100 CD34-PE-Vio770; Miltenyi Biotec).

    Article Title: Multiplex base editing of BCL11A regulatory elements to treat sickle cell disease.
    Article Snippet: .. Cells were harvested from BM, thymus, spleen, and blood, and stained with antibodies against the following murine and human surface markers: murine CD45 (1/50 mCD45-VioBlue; Miltenyi Biotec), human CD45 (1/50 hCD45-APCvio770; Miltenyi Biotec). .. BM cells were also stained with human CD3 (1/50 CD3-APC; Miltenyi Biotec), human CD14 (1/50 CD14-PECy7; BD Biosciences), human CD15 (1/50 CD15-PE; Miltenyi Biotec), human CD11b (1/100 CD11b-APC; Miltenyi Biotec), human CD19 (1/100 CD19-BV510; BD Biosciences), human CD235a (1/50 CD235a-PE; BD Biosciences), human CD71 (1/10 CD71-APC; BD Biosciences), CD36 (1/50 CD36-FITC; BD Bio-sciences), and CD34 (1/100 CD34-PE-Vio770; Miltenyi Biotec).

    other:

    Article Title: Dissecting the epigenetic regulation of the fetal hemoglobin genes to unravel a novel therapeutic approach for β-hemoglobinopathies
    Article Snippet: NBSGW mice were conditioned with busulfan (Sigma–Aldrich) injected intraperitoneally (15 mg/kg body weight) 24 h before transplantation.

    Purification:

    Article Title: Human B-cells can form hetero-aggregates with blood platelets: a novel insight into adaptive immunity regulation in multiple sclerosis.
    Article Snippet: Prostaglandin E1 (PGE1) (Sigma-Aldrich, USA) was added to the PRP at a final concentration of 1 μM to inhibit spontaneous platelet activation. .. To remove leukocyte and erythrocyte contamination, PRP was purified using MicroBeads conjugated to monoclonal human CD45 and CD235a antibodies, and MS MACS Columns (Miltenyi Biotec, USA), according to the manufacturer’s protocol. ..

    Magnetic Cell Separation:

    Article Title: Human B-cells can form hetero-aggregates with blood platelets: a novel insight into adaptive immunity regulation in multiple sclerosis.
    Article Snippet: Prostaglandin E1 (PGE1) (Sigma-Aldrich, USA) was added to the PRP at a final concentration of 1 μM to inhibit spontaneous platelet activation. .. To remove leukocyte and erythrocyte contamination, PRP was purified using MicroBeads conjugated to monoclonal human CD45 and CD235a antibodies, and MS MACS Columns (Miltenyi Biotec, USA), according to the manufacturer’s protocol. ..

    Isolation:

    Article Title: In vivo HSC transduction in humanized mice mediated by novel capsid-modified HDAd vectors
    Article Snippet: .. Human CD45 + and/or CD34 + cells from chimeric bone marrow were isolated using human CD45 or CD34 Microbeads (cat# 130-045-801, cat#130-046-702, respectively) (Miltenyi Biotec, San Diego, CA) according to the manufacturer’s instructions. ..

    Expressing:

    Article Title: Base editing of β 0 thalassemia mutations as a therapeutic strategy for β-hemoglobinopathies: efficacy and genotoxicity studies
    Article Snippet: .. Cells from PB and organs were harvested and analyzed for the presence of human CD45 + (using an anti-human CD45-VioBlue antibody, Miltenyi Biotec) cells and the expression of different lineage specific surface markers (using an anti-human CD235/GpA FITC antibody (Biolegend); an anti-human CD33-APC antibody (Miltenyi Biotec); an anti-human CD19-PECy7 antibody (BD); an anti-human CD3-BV510 (Biolegend); an anti-human CD34-PE antibody (BD); an anti-human CD71 APC antibody (BD Pharmingen); and analyzed by CANTO II flow-cytometer (BD) and FCS Express v7 Software (De Novo Software). .. Human CD45 + cells from BM were sorted by immunomagnetic negative selection with murine cells depletion Kit (Miltenyi Biotec).

    Software:

    Article Title: Base editing of β 0 thalassemia mutations as a therapeutic strategy for β-hemoglobinopathies: efficacy and genotoxicity studies
    Article Snippet: .. Cells from PB and organs were harvested and analyzed for the presence of human CD45 + (using an anti-human CD45-VioBlue antibody, Miltenyi Biotec) cells and the expression of different lineage specific surface markers (using an anti-human CD235/GpA FITC antibody (Biolegend); an anti-human CD33-APC antibody (Miltenyi Biotec); an anti-human CD19-PECy7 antibody (BD); an anti-human CD3-BV510 (Biolegend); an anti-human CD34-PE antibody (BD); an anti-human CD71 APC antibody (BD Pharmingen); and analyzed by CANTO II flow-cytometer (BD) and FCS Express v7 Software (De Novo Software). .. Human CD45 + cells from BM were sorted by immunomagnetic negative selection with murine cells depletion Kit (Miltenyi Biotec).



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    Tumor-infiltrating immune cells were isolated from vitally stored tumor tissue using CD45 MicroBeads as described in “Materials and Methods”. Spectral flow cytometry was done following staining against a panel of surface antigens to discriminate immune cell subtypes. A Gating strategy. B Quantification and stratification by patient’s outcome (dead/alive by data cutoff: July 31st 2025). Specific immune cell subtypes were identified by their expression profile. Given is the % of positive cells after gating of the viable immune cells with additional hierarchical sub-gating to distinguish lymphoid and myeloid compartments. For M1/M2-like macrophages, the ratio was calculated by dividing the value of CD3 - CD14 + macrophages by the value of CD3 - CD163 + M2-like macrophages. N = 25, Unpaired two-tailed Student’s t test or Mann–Whitney U test, * p < 0.05.

    Journal: Communications Medicine

    Article Title: Perivascular spaces are associated with T cell trafficking and may have prognostic relevance in brain metastases

    doi: 10.1038/s43856-026-01918-7

    Figure Lengend Snippet: Tumor-infiltrating immune cells were isolated from vitally stored tumor tissue using CD45 MicroBeads as described in “Materials and Methods”. Spectral flow cytometry was done following staining against a panel of surface antigens to discriminate immune cell subtypes. A Gating strategy. B Quantification and stratification by patient’s outcome (dead/alive by data cutoff: July 31st 2025). Specific immune cell subtypes were identified by their expression profile. Given is the % of positive cells after gating of the viable immune cells with additional hierarchical sub-gating to distinguish lymphoid and myeloid compartments. For M1/M2-like macrophages, the ratio was calculated by dividing the value of CD3 - CD14 + macrophages by the value of CD3 - CD163 + M2-like macrophages. N = 25, Unpaired two-tailed Student’s t test or Mann–Whitney U test, * p < 0.05.

    Article Snippet: Tumor-infiltrating immune cells were isolated from vitally stored tumor tissue using CD45 MicroBeads (Miltenyi Biotec, Cat: 130-118-780) according to the manufacturer’s protocol.

    Techniques: Isolation, Tissue, Flow Cytometry, Staining, Immune Cell, Expressing, Two Tailed Test, MANN-WHITNEY

    CD45 + and CD45 - cell fractions were examined for subtype-specific cell infiltration. Infiltration patterns were stratified by MRI-T2 weighted PVS grade in two distinct anatomic locations, i.e. ( A , C ) BG and ( B , D ) CSO. For CD45+ fractions, cytotoxicity and exhaustion-related markers were considered (CD3, CD8, PD1, CD183/CXCR3, Granzyme B, and IFNγ). In the CD45- fractions, markers associated with vascular and endothelial cells, involved in T cell trafficking (CD31/PECAM-1, CD54/ICAM-1, CXCL9/CXCL10, CD106/VCAM-1, and CD309/VEGFR2) were analyzed. Cell quantification was done by including the number of viable immune cells, positive for either marker. Given is the % of positive cells after gating of the viable immune cells with additional hierarchical sub-gating to distinguish cellular compartments and activation states. N = 16, Unpaired two-tailed Student’s t test or Mann–Whitney U test, * p < 0.05.

    Journal: Communications Medicine

    Article Title: Perivascular spaces are associated with T cell trafficking and may have prognostic relevance in brain metastases

    doi: 10.1038/s43856-026-01918-7

    Figure Lengend Snippet: CD45 + and CD45 - cell fractions were examined for subtype-specific cell infiltration. Infiltration patterns were stratified by MRI-T2 weighted PVS grade in two distinct anatomic locations, i.e. ( A , C ) BG and ( B , D ) CSO. For CD45+ fractions, cytotoxicity and exhaustion-related markers were considered (CD3, CD8, PD1, CD183/CXCR3, Granzyme B, and IFNγ). In the CD45- fractions, markers associated with vascular and endothelial cells, involved in T cell trafficking (CD31/PECAM-1, CD54/ICAM-1, CXCL9/CXCL10, CD106/VCAM-1, and CD309/VEGFR2) were analyzed. Cell quantification was done by including the number of viable immune cells, positive for either marker. Given is the % of positive cells after gating of the viable immune cells with additional hierarchical sub-gating to distinguish cellular compartments and activation states. N = 16, Unpaired two-tailed Student’s t test or Mann–Whitney U test, * p < 0.05.

    Article Snippet: Tumor-infiltrating immune cells were isolated from vitally stored tumor tissue using CD45 MicroBeads (Miltenyi Biotec, Cat: 130-118-780) according to the manufacturer’s protocol.

    Techniques: Marker, Activation Assay, Two Tailed Test, MANN-WHITNEY