alp  (R&D Systems)

Journal: Vaccines

Article Title: Cellular Immune Response and T Cell Epitope Mapping of Plasmodium falciparum Chimeric Vaccine Candidate GMZ2.6c and Its Components (MSP-3, GLURP and Pfs48/45) in Individuals Naturally Exposed to Malaria in Brazilian Amazon

doi: 10.3390/vaccines14050423

Figure Lengend Snippet: IFN-γ responses to MSP-3, GLURP, and Pfs48/45 synthetic peptides. Frequency of positive responders ( A ) and numbers of spots-forming units (SFU) ( B ) of IFN-γ responses to Mp 1 (blue), Ppp I (green), Ppp II (gray), and Gpp I (red) in exposed P. falciparum -infected (PFI, n = 20), non-infected (NI, n = 25), and non-endemic control (Control, n = 10) individuals. Bars represent the frequency of responders ( A ) and medians of adjusted SFU of IFN-γ responses to each peptide pool ( B ). Lines represent the interquartile ranges. Dashed red lines represent the positivity limit. Statistical significance was calculated using chi-square, Mann–Whitney test, and Kruskal–Wallis test, followed by Dunn’s post hoc test. Significant differences are indicated by * (* p < 0.05; *** p < 0.0005).

Article Snippet: ELISpot assays were carried out using the commercial kit ELISpot Plus: Human IFN-γ (ALP) (MabTech, Nacka Strand, Sweden) according to the manufacturer’s instructions.

Techniques: Infection, Control, MANN-WHITNEY

Journal: iScience

Article Title: Robust effector memory features of human T-bet hi B cells induced by repeated mRNA vaccination

doi: 10.1016/j.isci.2026.114804

Figure Lengend Snippet: RBD-specific T-bet hi B cells induced by multiple mRNA vaccinations are robustly affinity-matured and can rapidly differentiate into antibody-secreting cells (ASCs) (A) Gene set enrichment analysis (GSEA) showing that RBD-specific T-bet hi B cells are significantly enriched in gene sets related to effector functions and activation, compared with RBD-specific switched memory B cells. Dotted lines indicate false discovery rate (FDR) of 0.05. (B) Gating strategy for fluorescence-activated cell sorting (FACS) of T-bet hi and classical memory B cells. (C) Representative ASC frequencies among sorted T-bet hi and classical memory B cells at 48 h after in-vitro polyclonal stimulation with IL-2 and R848. (D) Summary of ASC frequencies after stimulation of sorted T-bet hi and classical memory B cells, showing more robust differentiation potentials of T-bet hi B cells. (E) Representative data from IgG ELISPOT with sorted memory B cell subpopulations. (F) Summary of Wuhan-Hu-1 RBD-specific IgG SFUs per 1000 total IgG SFUs normalized by RBD-specific B cell frequency of each subset between classical and T-bet hi B cells. (G) Summary of RBD-specific IgG SFUs per 1000 total IgG SFUs among stimulated classical memory, unstimulated classical memory and T-bet hi B cells. (H) Surrogate RBD neutralization capacity of antibodies secreted by memory B cell subsets, in terms of binding inhibition rates. (I) Heavy chain somatic hypermutation frequency of RBD-specific memory B cells 14 days after the 2 nd or 3 rd vaccination, showing significant and comparable affinity maturation of T-bet hi B cell. (J) LIBRA-seq scores calculated from RBD-tetramer ADT increases in both subpopulations following additional vaccine doses. Permutation test for GSEA terms in A, Wilcoxon rank-sum test for D–4H and Mann-Whitney U test for I and 4J, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., non-significant. Pooled data are represented as mean ± SD.

Article Snippet: ELISpot Flex: Human IgG (ALP) , MABTECH , Cat# 3850-2A.

Techniques: Activation Assay, Fluorescence, FACS, In Vitro, Enzyme-linked Immunospot, Neutralization, Binding Assay, Inhibition, MANN-WHITNEY