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aip4 sirna  (OriGene)


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    Structured Review

    OriGene aip4 sirna
    Aip4 Sirna, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aip/ARA9+(AIP)+Human+siRNA+Oligo+Duplex/pm39259704-339-0-5
    Average 92 stars, based on 1 article reviews
    aip4 sirna - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Amplification:

    Article Title: The QKI-6 RNA Binding Protein Regulates Actin-interacting Protein-1 mRNA Stability during Oligodendrocyte Differentiation
    Article Snippet: The gels were dried and the bound, and unbound RNAs were analyzed using a Storm PhosphorImager (GE Healthcare, Baie-d’Urfe, QC, Canada). .. The 3 -UTR of AIP-1 was amplified using the following primers (5 -AAT CAA GCT TAC GCA AAA ACA CTC CCA ATC-3 and 5 -AAT CAC TAG TCT CTG GAT GGA CCG AAT CAG-3 ) from the human AIP-1 full-length clone (Origene, Rockville, MD) and cloned into the pMIR-REPORT luciferase vector (Ambion). .. Human embryonic kidney (HEK)293 cells were cotransfected with either empty pMIR luciferase, the pLuc:AIP-1 3 -UTR, or the pLuc:AIP-1 3 -UTR with the mutated QRE along with either pcDNA (control), pcDNAmyc-QKI-6, or pcDNA-myc-QKI-6:V157E. pRLTK (Promega, Madison, WI) encoding Renilla luciferase was used to control transfection efficiency.

    Clone Assay:

    Article Title: The QKI-6 RNA Binding Protein Regulates Actin-interacting Protein-1 mRNA Stability during Oligodendrocyte Differentiation
    Article Snippet: The gels were dried and the bound, and unbound RNAs were analyzed using a Storm PhosphorImager (GE Healthcare, Baie-d’Urfe, QC, Canada). .. The 3 -UTR of AIP-1 was amplified using the following primers (5 -AAT CAA GCT TAC GCA AAA ACA CTC CCA ATC-3 and 5 -AAT CAC TAG TCT CTG GAT GGA CCG AAT CAG-3 ) from the human AIP-1 full-length clone (Origene, Rockville, MD) and cloned into the pMIR-REPORT luciferase vector (Ambion). .. Human embryonic kidney (HEK)293 cells were cotransfected with either empty pMIR luciferase, the pLuc:AIP-1 3 -UTR, or the pLuc:AIP-1 3 -UTR with the mutated QRE along with either pcDNA (control), pcDNAmyc-QKI-6, or pcDNA-myc-QKI-6:V157E. pRLTK (Promega, Madison, WI) encoding Renilla luciferase was used to control transfection efficiency.

    Luciferase:

    Article Title: The QKI-6 RNA Binding Protein Regulates Actin-interacting Protein-1 mRNA Stability during Oligodendrocyte Differentiation
    Article Snippet: The gels were dried and the bound, and unbound RNAs were analyzed using a Storm PhosphorImager (GE Healthcare, Baie-d’Urfe, QC, Canada). .. The 3 -UTR of AIP-1 was amplified using the following primers (5 -AAT CAA GCT TAC GCA AAA ACA CTC CCA ATC-3 and 5 -AAT CAC TAG TCT CTG GAT GGA CCG AAT CAG-3 ) from the human AIP-1 full-length clone (Origene, Rockville, MD) and cloned into the pMIR-REPORT luciferase vector (Ambion). .. Human embryonic kidney (HEK)293 cells were cotransfected with either empty pMIR luciferase, the pLuc:AIP-1 3 -UTR, or the pLuc:AIP-1 3 -UTR with the mutated QRE along with either pcDNA (control), pcDNAmyc-QKI-6, or pcDNA-myc-QKI-6:V157E. pRLTK (Promega, Madison, WI) encoding Renilla luciferase was used to control transfection efficiency.

    Plasmid Preparation:

    Article Title: The QKI-6 RNA Binding Protein Regulates Actin-interacting Protein-1 mRNA Stability during Oligodendrocyte Differentiation
    Article Snippet: The gels were dried and the bound, and unbound RNAs were analyzed using a Storm PhosphorImager (GE Healthcare, Baie-d’Urfe, QC, Canada). .. The 3 -UTR of AIP-1 was amplified using the following primers (5 -AAT CAA GCT TAC GCA AAA ACA CTC CCA ATC-3 and 5 -AAT CAC TAG TCT CTG GAT GGA CCG AAT CAG-3 ) from the human AIP-1 full-length clone (Origene, Rockville, MD) and cloned into the pMIR-REPORT luciferase vector (Ambion). .. Human embryonic kidney (HEK)293 cells were cotransfected with either empty pMIR luciferase, the pLuc:AIP-1 3 -UTR, or the pLuc:AIP-1 3 -UTR with the mutated QRE along with either pcDNA (control), pcDNAmyc-QKI-6, or pcDNA-myc-QKI-6:V157E. pRLTK (Promega, Madison, WI) encoding Renilla luciferase was used to control transfection efficiency.



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    Figure <t>3</t> <t>Immunohistochemistry</t> of pituitary tumor from index case. (a) The tumor, which stained strongly positive for growth hormone and prolactin (not shown) also had strong positive staining for c-erb. (b) Immunohistochemistry of the pituitary tumor for <t>AIP,</t> showing the reduced AIP staining seen in a region of tumor when compared with a section of normal pituitary.
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    Image Search Results


    Figure 3 Immunohistochemistry of pituitary tumor from index case. (a) The tumor, which stained strongly positive for growth hormone and prolactin (not shown) also had strong positive staining for c-erb. (b) Immunohistochemistry of the pituitary tumor for AIP, showing the reduced AIP staining seen in a region of tumor when compared with a section of normal pituitary.

    Journal: European Journal of Endocrinology

    Article Title: Variable pathological and clinical features of a large Brazilian family harboring a mutation in the aryl hydrocarbon receptor-interacting protein gene

    doi: 10.1530/eje-07-0533

    Figure Lengend Snippet: Figure 3 Immunohistochemistry of pituitary tumor from index case. (a) The tumor, which stained strongly positive for growth hormone and prolactin (not shown) also had strong positive staining for c-erb. (b) Immunohistochemistry of the pituitary tumor for AIP, showing the reduced AIP staining seen in a region of tumor when compared with a section of normal pituitary.

    Article Snippet: Antigen retrieval was performed by microwave boiling in citrate buffer pH 6.0 for two periods of 5 min and one period of 3 min. Immunohistochemistry was performed using a mouse anti-human AIP mAb at a 1:500 dilution (Novus Biological, DBA Italia s.r.l, Segrate, Italy) and a multilink biotinylated antibody and the avidin–biotin peroxidase system according to the manufacturer’s instructions (LSABC kit, DAKO Cytomation, Milan, Italy).

    Techniques: Immunohistochemistry, Staining