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htlr5  (InvivoGen)


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    Structured Review

    InvivoGen htlr5
    Htlr5, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htlr5+cells/HEK-Blue+hTLR5+cells/pm41962328-330-12-21
    Average 95 stars, based on 78 article reviews
    htlr5 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Suspension:

    Article Title: Constructing conjugate vaccine against Salmonella Typhimurium using lipid-A free lipopolysaccharide
    Article Snippet: .. A suspension of HEK-Blue hTLR5 cells (InvivoGen) was prepared at a concentration of 1.4 × 10 4 cells/mL in the HEK-Blue Detection medium. ..

    Article Title: Constructing conjugate vaccine against Salmonella Typhimurium using lipid-A free lipopolysaccharide.
    Article Snippet: .. Evaluation of hTLR5 activity by SEAP reporter cellular assay A suspension of HEK-Blue hTLR5 cells (InvivoGen) was prepared at a concentration of 1.4 × 104 cells/mL in the HEK-Blue Detection medium. ..

    Concentration Assay:

    Article Title: Constructing conjugate vaccine against Salmonella Typhimurium using lipid-A free lipopolysaccharide
    Article Snippet: .. A suspension of HEK-Blue hTLR5 cells (InvivoGen) was prepared at a concentration of 1.4 × 10 4 cells/mL in the HEK-Blue Detection medium. ..

    Article Title: Constructing conjugate vaccine against Salmonella Typhimurium using lipid-A free lipopolysaccharide.
    Article Snippet: .. Evaluation of hTLR5 activity by SEAP reporter cellular assay A suspension of HEK-Blue hTLR5 cells (InvivoGen) was prepared at a concentration of 1.4 × 104 cells/mL in the HEK-Blue Detection medium. ..

    Activity Assay:

    Article Title: Constructing conjugate vaccine against Salmonella Typhimurium using lipid-A free lipopolysaccharide.
    Article Snippet: .. Evaluation of hTLR5 activity by SEAP reporter cellular assay A suspension of HEK-Blue hTLR5 cells (InvivoGen) was prepared at a concentration of 1.4 × 104 cells/mL in the HEK-Blue Detection medium. ..

    Article Title: The response regulator FpsR controls the flagella-pili transition and mucosal colonization in Ligilactobacillus ruminis .
    Article Snippet: Finally, the samples were negatively stained with 2% ammonium molybdate and observed using a transmission electron microscope (H−7600, Hitachi). .. Assessment of proinflammatory activity using cultured cells Proinflammatory responses were assessed based on previously described methods,24,45 including quantification of IL−8 production by Caco−2 cells and a reporter gene assay using HEK-Blue hTLR5 cells (InvivoGen). .. Caco−2 cells were cultured in DMEM (Wako) supplemented with 10% fetal bovine serum (FBS), non-essential amino acids, GlutaMAX (Thermo Fisher Scientific), and penicillin/streptomycin.

    Article Title: An all-in-one adjuvanted therapeutic cancer vaccine targeting dendritic cell cytosol induces long-lived tumor suppression through NLRC4 inflammasome activation.
    Article Snippet: .. To test whether the EF or DEF proteins maintain TLR5-stimulating activities, we measured TLR5-dependent NF-κB-stimulating activity of the recombinant proteins by using HEK-BlueTM hTLR5 cells (InvivoGen, hκb-htlr-5) and HEK-BlueTM Detection (InvivoGen, hb-det2) assay systems following the manufacturer’s instructions. .. The EC50 was calculated using triplicate OD 620 nm values for each protein concentration over a wide range of protein concentrations (0.0375 nM–19.29 nM at the AAT S. Puth et al. Biomaterials 286 (2022) 121542 Bioquest website [https://www.aatbio.com/tools/ec50-calculator]).

    Cell Culture:

    Article Title: The response regulator FpsR controls the flagella-pili transition and mucosal colonization in Ligilactobacillus ruminis .
    Article Snippet: Finally, the samples were negatively stained with 2% ammonium molybdate and observed using a transmission electron microscope (H−7600, Hitachi). .. Assessment of proinflammatory activity using cultured cells Proinflammatory responses were assessed based on previously described methods,24,45 including quantification of IL−8 production by Caco−2 cells and a reporter gene assay using HEK-Blue hTLR5 cells (InvivoGen). .. Caco−2 cells were cultured in DMEM (Wako) supplemented with 10% fetal bovine serum (FBS), non-essential amino acids, GlutaMAX (Thermo Fisher Scientific), and penicillin/streptomycin.

    Reporter Gene Assay:

    Article Title: The response regulator FpsR controls the flagella-pili transition and mucosal colonization in Ligilactobacillus ruminis .
    Article Snippet: Finally, the samples were negatively stained with 2% ammonium molybdate and observed using a transmission electron microscope (H−7600, Hitachi). .. Assessment of proinflammatory activity using cultured cells Proinflammatory responses were assessed based on previously described methods,24,45 including quantification of IL−8 production by Caco−2 cells and a reporter gene assay using HEK-Blue hTLR5 cells (InvivoGen). .. Caco−2 cells were cultured in DMEM (Wako) supplemented with 10% fetal bovine serum (FBS), non-essential amino acids, GlutaMAX (Thermo Fisher Scientific), and penicillin/streptomycin.

    Article Title: The response regulator FpsR controls the flagella–pili transition and mucosal colonization in Ligilactobacillus ruminis
    Article Snippet: Finally, the samples were negatively stained with 2% ammonium molybdate and observed using a transmission electron microscope (H−7600, Hitachi). .. Proinflammatory responses were assessed based on previously described methods, including quantification of IL−8 production by Caco−2 cells and a reporter gene assay using HEK-Blue hTLR5 cells (InvivoGen). .. Caco−2 cells were cultured in DMEM (Wako) supplemented with 10% fetal bovine serum (FBS), non-essential amino acids, GlutaMAX (Thermo Fisher Scientific), and penicillin/streptomycin.

    Recombinant:

    Article Title: An all-in-one adjuvanted therapeutic cancer vaccine targeting dendritic cell cytosol induces long-lived tumor suppression through NLRC4 inflammasome activation.
    Article Snippet: .. To test whether the EF or DEF proteins maintain TLR5-stimulating activities, we measured TLR5-dependent NF-κB-stimulating activity of the recombinant proteins by using HEK-BlueTM hTLR5 cells (InvivoGen, hκb-htlr-5) and HEK-BlueTM Detection (InvivoGen, hb-det2) assay systems following the manufacturer’s instructions. .. The EC50 was calculated using triplicate OD 620 nm values for each protein concentration over a wide range of protein concentrations (0.0375 nM–19.29 nM at the AAT S. Puth et al. Biomaterials 286 (2022) 121542 Bioquest website [https://www.aatbio.com/tools/ec50-calculator]).



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    A The vector map of recombinant protein constructs: FMS-like tyrosine kinase-3 ligand extracellular domain (Flt3L/F), Vibrio vulnificus flagellin B (FlaB/B). The fusion protein was generated by combining F and B with B position at the C-terminal (FB) or the N-terminal (BF). B Characterization of the recombinant proteins by sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) and subsequent Western blot analysis using mouse anti-FlaB serum or rabbit anti-Flt3L antibody. C Determination of TLR5-dependent NF-κB stimulating activity of B, FB, and BF in various protein concentrations using HEK-Blue™ <t>hTLR5</t> cells ( n = 3 biological replicates). D Total cell number and the number of cell cluster formations of BMDCs after 9 days of cultures supplemented with B, F, and FB. Floating cells were collected and counted using a Vi-Cell Blue machine ( n = 7 biological replicates), while cell clusters were visualized with an EMOS5000 machine ( n = 5 biological replicates). E Representative flow histogram of CD103 expression on CD11c + MHCII hi population and quantification data of CD103 + DCs generation by recombination protein in a dose-dependent manner using BMDCs ( n = 3 biological replicates). F Representative levels of CFSE in CD8 + T cells from cultures with the indicated DCs, the individual peaks of CFSE dilution gated, and quantitative proliferation index of CD8 + T cell. For all sections: **** P < 0.0001; *** P < 0.001; ** P < 0.01; * P < 0.05.
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    Image Search Results


    A The vector map of recombinant protein constructs: FMS-like tyrosine kinase-3 ligand extracellular domain (Flt3L/F), Vibrio vulnificus flagellin B (FlaB/B). The fusion protein was generated by combining F and B with B position at the C-terminal (FB) or the N-terminal (BF). B Characterization of the recombinant proteins by sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) and subsequent Western blot analysis using mouse anti-FlaB serum or rabbit anti-Flt3L antibody. C Determination of TLR5-dependent NF-κB stimulating activity of B, FB, and BF in various protein concentrations using HEK-Blue™ hTLR5 cells ( n = 3 biological replicates). D Total cell number and the number of cell cluster formations of BMDCs after 9 days of cultures supplemented with B, F, and FB. Floating cells were collected and counted using a Vi-Cell Blue machine ( n = 7 biological replicates), while cell clusters were visualized with an EMOS5000 machine ( n = 5 biological replicates). E Representative flow histogram of CD103 expression on CD11c + MHCII hi population and quantification data of CD103 + DCs generation by recombination protein in a dose-dependent manner using BMDCs ( n = 3 biological replicates). F Representative levels of CFSE in CD8 + T cells from cultures with the indicated DCs, the individual peaks of CFSE dilution gated, and quantitative proliferation index of CD8 + T cell. For all sections: **** P < 0.0001; *** P < 0.001; ** P < 0.01; * P < 0.05.

    Journal: NPJ Vaccines

    Article Title: Antigen cross-presentation potentiating cancer vaccine adjuvant for T cell expansion and synergy with anti-PD-1

    doi: 10.1038/s41541-026-01376-1

    Figure Lengend Snippet: A The vector map of recombinant protein constructs: FMS-like tyrosine kinase-3 ligand extracellular domain (Flt3L/F), Vibrio vulnificus flagellin B (FlaB/B). The fusion protein was generated by combining F and B with B position at the C-terminal (FB) or the N-terminal (BF). B Characterization of the recombinant proteins by sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) and subsequent Western blot analysis using mouse anti-FlaB serum or rabbit anti-Flt3L antibody. C Determination of TLR5-dependent NF-κB stimulating activity of B, FB, and BF in various protein concentrations using HEK-Blue™ hTLR5 cells ( n = 3 biological replicates). D Total cell number and the number of cell cluster formations of BMDCs after 9 days of cultures supplemented with B, F, and FB. Floating cells were collected and counted using a Vi-Cell Blue machine ( n = 7 biological replicates), while cell clusters were visualized with an EMOS5000 machine ( n = 5 biological replicates). E Representative flow histogram of CD103 expression on CD11c + MHCII hi population and quantification data of CD103 + DCs generation by recombination protein in a dose-dependent manner using BMDCs ( n = 3 biological replicates). F Representative levels of CFSE in CD8 + T cells from cultures with the indicated DCs, the individual peaks of CFSE dilution gated, and quantitative proliferation index of CD8 + T cell. For all sections: **** P < 0.0001; *** P < 0.001; ** P < 0.01; * P < 0.05.

    Article Snippet: To assess the TLR5-stimulating activities of the recombinant fusion proteins, we evaluated the TLR5-dependent NF-κB-stimulating activity of the recombinant protein using HEK-BlueTM hTLR5 cells (hκb-help-5, InvivoGen, USA) and HEK-BlueTM Detection (hb-det2, InvivoGen, USA) assay systems following the manufacturer’s instructions.

    Techniques: Plasmid Preparation, Recombinant, Construct, Generated, SDS Page, Western Blot, Activity Assay, Expressing