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Adipose triglyceride lipase (ATGL), but not hormone‐sensitive lipase (HSL), contributes to the anti‐contractile effect of PVAT. Concentration‐response curves to phenylephrine (PE), with or without Atglistatin (10 μmol/L) or BAY 59–9435 (100 nmol/L), in PVAT intact (+PVAT) or PVAT denuded (‐PVAT) mesenteric resistance arteries from male Wistar rats. Mean ± SEM; n = 4–12. Non‐linear regression analysis ( E max ): ** p < 0.01, *** p < 0.001.

Journal: Microcirculation (New York, N.y. : 1994)

Article Title: Adipose Triglyceride Lipase and Gpr40 Contribute to the Anti‐Contractile Effect of Perivascular Adipose Tissue

doi: 10.1111/micc.70063

Figure Lengend Snippet: Adipose triglyceride lipase (ATGL), but not hormone‐sensitive lipase (HSL), contributes to the anti‐contractile effect of PVAT. Concentration‐response curves to phenylephrine (PE), with or without Atglistatin (10 μmol/L) or BAY 59–9435 (100 nmol/L), in PVAT intact (+PVAT) or PVAT denuded (‐PVAT) mesenteric resistance arteries from male Wistar rats. Mean ± SEM; n = 4–12. Non‐linear regression analysis ( E max ): ** p < 0.01, *** p < 0.001.

Article Snippet: Pharmacological modulators used in this study included: ATGL inhibitor Atglistatin (Millipore Sigma; 10 μmol/L), HSL inhibitor BAY 59‐9435 (MedChemExpress; 100 nmol/L), CPT1 inhibitor Etomoxir (Millipore Sigma; 10 μmol/L), Gpr40 antagonist GW 1100 (Millipore Sigma; 10 μmol/L), Gpr120 antagonist AH 7614 (Millipore Sigma; 10 μmol/L), Gpr40 agonist LY2922470 (MedChemExpress; 100 nmol/L), dual Gpr40/Gpr120 agonist GW‐9508 (Tocris; 10 μmol/L), and the β‐arrestin/β2‐adaptin interaction inhibitor Barbadin (MedChemExpress; 50 μmol/L).

Techniques: Concentration Assay