Journal: Communications Biology

Article Title: Omega-6 highly unsaturated fatty acids in Leydig cells facilitate male sex hormone production

doi: 10.1038/s42003-022-03972-y

Figure Lengend Snippet: a The lipase activity of recombinant mouse hormone-sensitive lipase (mHSL). HEK293T cells were transfected with empty vector (mock) or mHSL-expressing vector (mHSL) and the cell lysate (1 mg/mL) was incubated with each cholesterol ester species (100 μM) for 30 min at 37 °C. FFA, free fatty acid. n = 3 for each group. b HSL-dependent FFA liberation upon human chorionic gonadotropin (hCG) stimulation in MA-10 cells. Triacsin C was pretreated to block the conversion of FFAs to fatty acyl-CoAs. The FFA levels insensitive to HSL-IN-1 were subtracted to evaluate HSL-dependent FFA production. n = 3 for each group. Significance is based on unpaired two-tailed t -test with Welch’s correction. Data shown are the mean ± SEM. The detailed data are shown in Supplementary Fig. .

Article Snippet: HSL-IN-1 was purchased from MedchemExpress LLC., Monmouth Junction, USA.

Techniques: Activity Assay, Recombinant, Transfection, Plasmid Preparation, Expressing, Incubation, Blocking Assay, Two Tailed Test

Journal: Applied Microbiology and Biotechnology

Article Title: Discovery of psoralen as a quorum sensing inhibitor suppresses Pseudomonas aeruginosa virulence

doi: 10.1007/s00253-024-13067-9

Figure Lengend Snippet: Structure of P. aeruginosa QS signal molecules and psoralen. Native signal molecules 3-oxo-C12-HSL ( a ), C4-HSL ( b ), PQS ( c ) of P . aeruginosa QS system. d Psoralen. e The growth of PAO1 in MHB containing different concentrations of psoralen (37 °C, 18 h). f The growth of PAO1 in LB broth containing different concentrations of psoralen (37 °C, 24 h). g The growth curves of PAO1 in M9-adenosine (0.1%, w/v) containing different concentrations of psoralen (37 °C, 36 h). The results were presented as mean ± SD and three independent experiments. The analysis method was used one-way ANOVA; ns, not significant

Article Snippet: The QS signal molecules C12-HSL, C4-HSL, and PQS were purchased from MedChemExpress (Shanghai, China).

Techniques:

Journal: Applied Microbiology and Biotechnology

Article Title: Discovery of psoralen as a quorum sensing inhibitor suppresses Pseudomonas aeruginosa virulence

doi: 10.1007/s00253-024-13067-9

Figure Lengend Snippet: Effects of psoralen (200 μM) supplementation with AIs on phenotypic experiments of P . aeruginosa . a The growth of PAO1 in M9-adenosine (0.1%, w/v) medium containing psoralen supplementation C12-HSL. b The growth of PAO1 in M9-casein (0.5%, w/v) medium containing psoralen supplementation with different AIs. c The pyocyanin production of PAO1 in LB broth containing psoralen supplementation with different AIs. The effects of psoralen supplementation with different AIs on motility of PAO1 ( d , e ). d Swimming motility. e Twitching motility. f The biofilm formation of PAO1 containing psoralen supplementation with different AIs. g The image of motility of PAO1 containing psoralen supplementation with different AIs. The results were presented as mean ± SD and three independent experiments. The analysis method was used one-way ANOVA; ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: The QS signal molecules C12-HSL, C4-HSL, and PQS were purchased from MedChemExpress (Shanghai, China).

Techniques: