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hsf cells  (ATCC)


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    ATCC hsf cells
    Hsf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsf cells/product/ATCC
    Average 95 stars, based on 476 article reviews
    hsf cells - by Bioz Stars, 2026-06
    95/100 stars

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    In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of <t>HSF</t> <t>and</t> <t>MDA‐MB‐231</t> cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).
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    ATCC cell culture human skin fibroblast hsfs
    In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of <t>HSF</t> <t>and</t> <t>MDA‐MB‐231</t> cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).
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    In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of HSF and MDA‐MB‐231 cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).

    Journal: Advanced Science

    Article Title: Intelligent Thermo‐Self‐Limited Magnetothermia with Heat‐Triggered TERT Silencing for Precision Synergetic Cancer Therapy

    doi: 10.1002/advs.202519168

    Figure Lengend Snippet: In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of HSF and MDA‐MB‐231 cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).

    Article Snippet: MDA‐MB‐231 and HSF cells were purchased from Procell Life Science & Technology Co., Ltd (Wuhan, China).

    Techniques: In Vitro, Activation Assay, Staining, Expressing, Western Blot, Real-time Polymerase Chain Reaction