Journal: Endocrinology

Article Title: Female Mice with HSD17B1 Inactivation Show Mild Hyperandrogenism without Notable Impact on Reproductive Function or Bone

doi: 10.1210/endocr/bqaf167

Figure Lengend Snippet: Human and mouse HSD17B1 substrate binding modes. (A) 2D representations of E1 (substrate) and E2 (product). (B) The minimized human E1-HSD17B1 complex indicates that the C3-hydroxyl can form a double H-bond with both Glu283 and His222, and the C17-keto group can also optimally H-bond with Ser143 and Tyr156. (C) After the E1-to-E2 reaction, the C17-hydroxyl positioning of E2 at the catalytic end is compromised due to the proximity of multiple H-bond donors. (D) In mouse HSD17B1, Gly222 is present at the noncatalytic end of the enzyme, instead of the histidine, but the carboxylate group of Glu283 can H-bond directly with the C3-hydroxyl of E1. (E) Mutating Ser143 to alanine compromises or abolishes the C17-keto coordination needed for acquiring the proton from NAD (P)H via the phenol ring of Tyr156. (F) 2D representations of A4 (substrate) and T (product). (G) With the minimized human A4-HSD17B1 complex, the C17-keto group H-bonds with the epsilon protonated His222, preferring a binding mode that is reverse and nonproductive for the reaction. (H) Likewise, in the energy-minimized human T-HSD17B1 complex, the C17-hydroxyl also acquires the double H-bond arrangement with Glu283 and His222. (I) In the modeled mouse A4-HSD17B1 complex, the reverse binding mode is not favored as there are no suitable H-bond donors present at the noncatalytic end. The protein residues are shown as stick models with grey backbones. The ligands and cofactors (NADP+) are shown as ball-and-stick models with magenta/blue or green backbone, respectively. Human and mouse HSD17B1 residue numbering is taken from the UniProt entries DHB1_HUMAN ( P14061 ) and DHB1_MOUSE ( P51656 ). Abbreviations: A4, androstenedione; E1, estrone; E2, estradiol; HSD17B1, 17β-hydroxysteroid dehydrogenase 1; NADP, nicotinamide adenine dinucleotide phosphate; T, testosterone.

Article Snippet: Briefly, the point mutation was introduced to a wild-type (WT) mouse Hsd17b1 expression plasmid (Origene) with a Q5 Site Directed Mutagenesis Kit (NE Biolabs) according to manufacturer instructions, with the following primers: forward 5′-GTGACCGCGGCAGTGGGAGGCTT-3′, reverse 5′-CAGCACACGCCCAGAGTGGC-3′.

Techniques: Binding Assay, Residue

Journal: Endocrinology

Article Title: Female Mice with HSD17B1 Inactivation Show Mild Hyperandrogenism without Notable Impact on Reproductive Function or Bone

doi: 10.1210/endocr/bqaf167

Figure Lengend Snippet: HSD17B1 inactivating mutation affected ovarian steroidogenesis. (A) Conversion of E1 to E2 in MCF-7 cells transfected with Ser143Ala mutant (Mut) HSD17B1 is greatly impaired in comparison with cells transfected with WT HSD17B1, being equal to nontransfected cells (Sham). (B) No change was observed in Hsd17b1 mRNA expression in adult mouse ovaries analyzed by quantitative PCR (n = 4). (C) Intraovarian E1, A4, and DHT concentrations were increased in 6-month-old females, but other steroids were unchanged (n = 13). (D) Serum E1, A4, and T concentrations were also increased in the same animals. (E) Serum LH concentrations were increased in 6- to 7-month-old females (WT n = 11, HSD17B1-KI n = 12). (F) Ovary/serum steroid concentration ratio of A4 was increased, and T decreased in the HSD17B1-KI females. (G) The ratio of T to A4 was decreased in the HSD17B1-KI ovary but unchanged in serum. In (A), data are presented as means and SD; in (B-F), data are presented as individual values, with lines indicating means; and in (C, D, F), data were log 2 -transformed prior to analysis. *= P < .05, **= P < .01, ***= P < .001. Abbreviations: A4, androstenedione; DHT, dihydrotestosterone; E1, estrone; E2, estradiol; HSD17B1, 17β-hydroxysteroid dehydrogenase 1; HSD17B1-KI, 17β-hydroxysteroid dehydrogenase 1 Ser143Ala knock-in; T, testosterone; WT, wild-type.

Article Snippet: Briefly, the point mutation was introduced to a wild-type (WT) mouse Hsd17b1 expression plasmid (Origene) with a Q5 Site Directed Mutagenesis Kit (NE Biolabs) according to manufacturer instructions, with the following primers: forward 5′-GTGACCGCGGCAGTGGGAGGCTT-3′, reverse 5′-CAGCACACGCCCAGAGTGGC-3′.

Techniques: Mutagenesis, Transfection, Comparison, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Transformation Assay, Knock-In

Journal: Endocrinology

Article Title: Female Mice with HSD17B1 Inactivation Show Mild Hyperandrogenism without Notable Impact on Reproductive Function or Bone

doi: 10.1210/endocr/bqaf167

Figure Lengend Snippet: Female development and reproduction were not overtly disturbed by HSD17B1 inactivation. (A) Anogenital distance did not differ between WT (n = 13) and HSD17B1-KI (n = 13) females at the ages of 5 and 8 weeks, nor did (B) the timing of the onset of puberty. (C) Although the HSD17B1-KI females cycled mostly normally, a small increase was seen in the number of total days spent in the metestrus stage over the 4 cycles followed. (D) Representative diagrams of estrous cycle progression. (E) Mean time between spontaneous delivery of litters was delayed in HSD17B1-KI females in constant breeding for 2 months (n = 10). (F) Number of pups in litters after spontaneous delivery did not differ between the groups, nor did (G) weights of pups over the first 3 weeks after birth. (H) Ovary weights did not differ between WT and HSD17B1-KI females at 2 months of age (WT n = 10, HSD17B1-KI n = 11) but were decreased in HSD17B1-KI at 6 months of age (n = 10). (I) Representative ovarian histology, showing current and regressing corpora lutea and the prominent interstitial cells in HSD17B1-KI in comparison to WT histology. (J) Weights of ovaries did not differ between groups (n = 7) after stimulation with PMSG and hCG, nor did (K) the number of released oocytes after superovulation. In (A, B, E, F, H, J, K), data are presented as individual values, with lines indicating the means; in (C, G), data are presented as means and SD. In (I), scale bars in wider field images indicate 200 µm and in closer images 100 µm. * P < .05, ** P < .01. Abbreviations: hCG, human chorionic gonadotropin; HSD17B1, 17β-hydroxysteroid dehydrogenase 1; HSD17B1-KI, 17β-hydroxysteroid dehydrogenase 1 Ser143Ala knock-in; PMSG, pregnant mare serum gonadotropin; WT, wild-type.

Article Snippet: Briefly, the point mutation was introduced to a wild-type (WT) mouse Hsd17b1 expression plasmid (Origene) with a Q5 Site Directed Mutagenesis Kit (NE Biolabs) according to manufacturer instructions, with the following primers: forward 5′-GTGACCGCGGCAGTGGGAGGCTT-3′, reverse 5′-CAGCACACGCCCAGAGTGGC-3′.

Techniques: Comparison, Knock-In

Journal: Endocrinology

Article Title: Female Mice with HSD17B1 Inactivation Show Mild Hyperandrogenism without Notable Impact on Reproductive Function or Bone

doi: 10.1210/endocr/bqaf167

Figure Lengend Snippet: Ovarian gene expression was affected by HSD17B1 inactivation. (A) Relative ovarian expression of Star mRNA was increased in 2-month-old HSD17B1-KI females at the estrus stage of the cycle (n = 5) but not at 4 (WT n = 9, HSD17B1/KI n = 11) or 6 months (WT n = 8, HSD17B1-KI n = 7). (B) An increasing trend was visible in HSD17B1-KI ovary Cyp17a1 expression in the same animals and was significant at 6 months. (C) mRNA sequencing revealed an upregulation of Hsd3b6 in 6-month-old HSD17B1-KI, whereas (D) Hsd17b2 was downregulated. (E) Ar , as well as (F) androgen-responsive genes Sult1e1 , Insl3 , Nr5a1 , and Vcam1, were also observed to be upregulated in mRNA sequencing data. (G) STRING interaction analysis and clustering of the differentially expressed genes in 6-month-old females further revealed several clusters of interest upregulated in HSD17B1-KI. In (A-F), data are presented as individual values, with lines indicating the means. * P < .05, ** P < .01, *** P < .001. Abbreviations: HSD17B1, 17β-hydroxysteroid dehydrogenase 1; HSD17B1-KI, 17β-hydroxysteroid dehydrogenase 1 Ser143Ala knock-in; WT, wild-type.

Article Snippet: Briefly, the point mutation was introduced to a wild-type (WT) mouse Hsd17b1 expression plasmid (Origene) with a Q5 Site Directed Mutagenesis Kit (NE Biolabs) according to manufacturer instructions, with the following primers: forward 5′-GTGACCGCGGCAGTGGGAGGCTT-3′, reverse 5′-CAGCACACGCCCAGAGTGGC-3′.

Techniques: Gene Expression, Expressing, Sequencing, Knock-In

Journal: Endocrinology

Article Title: Female Mice with HSD17B1 Inactivation Show Mild Hyperandrogenism without Notable Impact on Reproductive Function or Bone

doi: 10.1210/endocr/bqaf167

Figure Lengend Snippet: Bone parameters were unchanged in females with HSD17B1 inactivation. (A) Representative reconstructed micro-computed tomography images of bone microstructure in WT and HSD17B1-KI femur. Blue represents the highest tissue mineral density. (B) Femur and tibia length did not differ between WT and HSD17B1-KI females at 2 (WT n = 10, HSD17B1-KI n = 11) or 6 months of age (WT n = 10, HSD17B1-KI n = 10). (C) No differences were observed in femur diameter (sagittal or coronal) at 2 months or 6 months either. (D) Representative images of TRAcP staining of the tibia of WT and HSD17B1-KI females at the age of 6 months, with arrows indicating multinucleated TRAcP-positive osteoclasts below the growth plate, with (E) showing the similar number of osteoclasts counted in histological sections at 2 months or 6 months in both WT and HSD17B1-KI females. In (E), scale bars in wider field pictures represent 1000 µm and in closer pictures 200 µm. In (B, C, E), data are presented as individual values, with lines indicating the means. * P < .05, ** P < .01, *** P < .001. Abbreviations: HSD17B1, 17β-hydroxysteroid dehydrogenase 1; HSD17B1-KI, 17β-hydroxysteroid dehydrogenase 1 Ser143Ala knock-in; TRAcP, tartrate-resistant acid phosphatase; WT, wild-type.

Article Snippet: Briefly, the point mutation was introduced to a wild-type (WT) mouse Hsd17b1 expression plasmid (Origene) with a Q5 Site Directed Mutagenesis Kit (NE Biolabs) according to manufacturer instructions, with the following primers: forward 5′-GTGACCGCGGCAGTGGGAGGCTT-3′, reverse 5′-CAGCACACGCCCAGAGTGGC-3′.

Techniques: Micro-CT, Staining, Knock-In

Journal: Endocrine Connections

Article Title: 17β-Hydroxysteroid dehydrogenase type I and aromatase in ovarian cortical inclusion cysts

doi: 10.1530/EC-24-0643

Figure Lengend Snippet: Micrograph of sections of a CIC lined with mixed epithelium stained with hematoxylin and eosin. (A) CIC with flat epithelium displays regions of ciliated cells. (B) Transition from flat to mucinous epithelia is observed in CIC mix . Sections of the same cortical inclusion cyst (CIC) lined with tubal-like epithelium (CIC tube ) show immunoreactivity for: (C) 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1), (D) aromatase and (E) ERα. The labeling for aromatase is lighter than that for HSD17B1 and ERα reactivity. Scale bar = 50 μm.

Article Snippet: The primary antibodies used were the HSD17B1 antibody (cat. GTX12312; GeneTex, USA), aromatase antibody H4 (cat. OASA02666; Aviva Systems Biology, USA) and Erα HC20 antibody (cat. sc-543; Santa Cruz Biotechnology, Inc., USA).

Techniques: Staining, Labeling

Journal: Endocrine Connections

Article Title: 17β-Hydroxysteroid dehydrogenase type I and aromatase in ovarian cortical inclusion cysts

doi: 10.1530/EC-24-0643

Figure Lengend Snippet: Frequency of positive immunoreactivity of 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1), aromatase and ERα in the ovarian surface epithelium (OSE) and cortical inclusion cyst (CIC).

Article Snippet: The primary antibodies used were the HSD17B1 antibody (cat. GTX12312; GeneTex, USA), aromatase antibody H4 (cat. OASA02666; Aviva Systems Biology, USA) and Erα HC20 antibody (cat. sc-543; Santa Cruz Biotechnology, Inc., USA).

Techniques:

Journal: Endocrine Connections

Article Title: 17β-Hydroxysteroid dehydrogenase type I and aromatase in ovarian cortical inclusion cysts

doi: 10.1530/EC-24-0643

Figure Lengend Snippet: Frequency of positive immunoreactivity of 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1), aromatase and ERα in cortical inclusion cyst (CIC) and ovarian surface epithelium (OSE) related to medically indicated oophorectomy.

Article Snippet: The primary antibodies used were the HSD17B1 antibody (cat. GTX12312; GeneTex, USA), aromatase antibody H4 (cat. OASA02666; Aviva Systems Biology, USA) and Erα HC20 antibody (cat. sc-543; Santa Cruz Biotechnology, Inc., USA).

Techniques:

Journal: Endocrine Connections

Article Title: 17β-Hydroxysteroid dehydrogenase type I and aromatase in ovarian cortical inclusion cysts

doi: 10.1530/EC-24-0643

Figure Lengend Snippet: Frequency of positive immunoreactivity of 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1), aromatase and ERα in variation of cortical inclusion cyst epithelium with OSE-like (CIC ose ) or epithelium of the uterine tube-like (CIC tube ).

Article Snippet: The primary antibodies used were the HSD17B1 antibody (cat. GTX12312; GeneTex, USA), aromatase antibody H4 (cat. OASA02666; Aviva Systems Biology, USA) and Erα HC20 antibody (cat. sc-543; Santa Cruz Biotechnology, Inc., USA).

Techniques: