Journal: Journal of translational medicine

Article Title: An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer.

doi: 10.1186/s12967-023-04048-z

Figure Lengend Snippet: Fig. 5 Kaempferol modulated estrogen metabolism pathways and differentially regulates PPARG expression in EC cells of different ER subtypes. A– B HSD17B1 and HSD17B1-associated genes, such as ESRRA, PPARG, and ESR1, are involved in several estrogen metabolism pathways, such as steroid binding, 17- beta-hydroxysteroid dehydrogenase (NADP+) activity, steroid hormone biosynthesis, and regulation of hormone levels. C Kaempferol suppressed the expression of PPARG in ER-positive AN3 CA and promoted the expression of PPARG in ER-negative HEC-1-A. D–I Kaempferol suppressed the expression of PPARGC1A and ESRRA in both AN3 CA (D–F) and HEC-1-A cells (G–I), without modulating ESR1. Western blotting (D–E and G–H) and the IHC scores (F and I) confirmed the differential expression of PPARGC1A and ESRRA. Results are presented as means and SDs. Compared with the negative control, *, #P < 0.05, **, ##P < 0.01, ***, ###P < 0.001

Article Snippet: The whole cell lysates and tumor homogenates (50 μg) were resolved on an 8 ~ 12% SDS–polyacrylamide gel, transferred to a polyvinylidene difluoride membrane (NEN Life Sciences, Boston, MA), probed sequentially with antibodies against ESR1 (ab108398, 67 kDa), ESRRA (ab137489, 55 kDa), PPARGC1A (ab188102, 91 kDa) (Abcam, Cambridge, MA, U. S.), CASP3/p17/p19 (19677–1, 35 kDa), CASP9/p35/p10 (66169–1, 46 kDa), PPARG (16643–1, 55 kDa), HSD17B1 (25334–1, 35 kDa) (Proteintech, Wuhan, China) at 4 °C overnight, rinsed, and incubated with the goat anti-rabbit secondary antibody (Abcam, Cambridge, MA).

Techniques: Expressing, Binding Assay, Activity Assay, Western Blot, Quantitative Proteomics, Negative Control

Journal: Oncotarget

Article Title: Estrogen metabolism in the human lung: impact of tumorigenesis, smoke, sex and race/ethnicity

doi: 10.18632/oncotarget.22269

Figure Lengend Snippet: qRT-PCR gene-specific primers (Life Technologies)

Article Snippet: HSD17B1 , Hs00166219_g1.

Techniques:

Journal: Ecotoxicology and environmental safety

Article Title: Diesel exhaust particle exposure exacerbates ciliary and epithelial barrier dysfunction in the multiciliated bronchial epithelium models.

doi: 10.1016/j.ecoenv.2024.116090

Figure Lengend Snippet: Fig. 2. DEPs change the ratio of ciliated and goblet cells in ALI-cultured NHBEs. (A) Experimental design of DEP treatment for 72 h on an apical side of the Transwell inserts. (B) Paraffin section of an ALI-cultured NHBEs exposed to DEPs was stained with H&E (left) and Alcian blue (right). Arrows and arrowheads indicate ciliated cells and goblet cells, respectively. (C) Representative immunofluorescence images of acetylated α-tubulin (green) and MUC5AC (red) after DEP treatment for 72 h. Bar: 20 μm. (D-F) Quantitative assessment of cilia and goblet cells-positive area in DEP-treated ALI-cultured NHBEs (n=3). Acetylated α-tubulin (D) and MUC5AC (E) positive area of Transwell membrane were measured with image J software and calculated as the percentage of MUC5AC / cilia area (F). n.s. > 0.05, *P < 0.05, **P < 0.01 and ***P < 0.001, compared with vehicle.

Article Snippet: Cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min and blocked with 1% bovine serum albumin (BSA) in PBS+0.1% Tween 20 (PBST) for 30 min. Primary antibodies including ARL13B (17711–1-AP; Proteintech, Rosemont, IL, USA), acetylated α-tubulin (sc-23950; Santa Cruz Biotechnology, Santa Cruz, CA, USA), E-cadherin (#3195; Cell signaling, Denver, MA, USA), phalloidin (A12381; Invitrogen, Carlsbad, CA, USA) and MUC5AC (orb547405; Biorbyt, Cambridge, UK) were diluted with 1% BSA in PBST and incubated with cells for 1 h at room temperature.

Techniques: Cell Culture, Paraffin Section, Staining, Immunofluorescence, Membrane, Software