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human lgg cell lines hs683  (ATCC)


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    ATCC human lgg cell lines hs683
    Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in <t>HS683</t> and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Human Lgg Cell Lines Hs683, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hs683+cell+lines/Hs+683/pmc13217492-249-1-22
    Average 95 stars, based on 341 article reviews
    human lgg cell lines hs683 - by Bioz Stars, 2026-10
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    1) Product Images from "Integrating multi-omics analysis and machine learning to refine molecular subtypes and prognostic assessment of lower-grade glioma"

    Article Title: Integrating multi-omics analysis and machine learning to refine molecular subtypes and prognostic assessment of lower-grade glioma

    Journal: Molecular Therapy Oncology

    doi: 10.1016/j.omton.2026.201227

    Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in HS683 and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Figure Legend Snippet: Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in HS683 and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Techniques Used: Expressing, Functional Assay, Biomarker Discovery, Western Blot, Knockdown, Migration, Wound Healing Assay, Cell Counting, Flow Cytometry

    Related Articles

    Cell Culture:

    Article Title: Biglycan Promotes Glioma Cell Line Epithelial-Mesenchymal Transition and Temozolomide Resistance in Vitro
    Article Snippet: .. U251MG and HS683 cell lines were bought from American Type Culture Collection (ATCC, Gaithersburg, MD, USA), cultured in DMEM (Gibco, USA) containing 10% fetal bovine serum (FBS; Sijiqing, Hangzhou China) and 100 U/ml penicillin, 100 μM streptomycin (Gibco, USA) at 37 °C with 5% CO2. .. BGN non-targeting control siRNA(siNC) and siRNA from GenePharma Inc (Shanghai, China) were used to establish different BGN expression U251MG and HS683.

    Article Title: Oncometabolite 2-hydroxyglutarate destabilizes CDH1 to regulate quiescence and enhance chemotherapy sensitivity.
    Article Snippet: .. U87MG, A549, HCT116, HEK293T, U251MG and HS683 cell lines were obtained from American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin– streptomycin at 37 °C in a 5% CO2 incubator. ..

    Article Title: SETMAR isoforms in glioblastoma: A matter of protein stability
    Article Snippet: .. U-87 MG, T98G, CRL 2020, A172 and Hs683 cell lines were cultured as recommended by ATCC, and 42MGBA was cultured as recommended by the Liebniz Institute DSMZ German Collection of Microorganisms and Cell Cultures. ..



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    human lgg cell lines hs683  (ATCC)
    95
    ATCC human lgg cell lines hs683
    Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in <t>HS683</t> and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Human Lgg Cell Lines Hs683, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hs683+cell+lines/Hs+683/pmc13217492-249-1-22
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    hs683 cell lines  (ATCC)
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    ATCC hs683 cell lines
    Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in <t>HS683</t> and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Hs683 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lgg cell lines hs683  (ATCC)
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    ATCC lgg cell lines hs683
    Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in <t>HS683</t> and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Lgg Cell Lines Hs683, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hs683+cell+lines/Hs+683/pm42014657-104-9-24
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    lgg cell lines hs683 - by Bioz Stars, 2026-10
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    human glioma cell lines hs683  (ATCC)
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    ATCC human glioma cell lines hs683
    Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in <t>HS683</t> and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Human Glioma Cell Lines Hs683, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell culture human lgg cell lines hs683  (ATCC)
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    ATCC cell culture human lgg cell lines hs683
    Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in <t>HS683</t> and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Cell Culture Human Lgg Cell Lines Hs683, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hs683 htb 138 human oligodendroglioma cell line  (ATCC)
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    Knockdown of spermine synthase (SMS) inhibits the malignant behavior of lower-grade glioma cells. A : Western blot analysis of SMS protein expression levels in human normal glial cell lines CP-H121/122/123 and glioma cell line <t>HS683/U251,</t> with statistical analysis on the right. B : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by Western blot, with protein expression analysis on the right. C : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by quantitative reverse transcription polymerase chain reaction. D : Cell proliferation before and after SMS knockdown was measured by CCK-8 assay. E : The apoptosis ratio of cells before and after SMS knockdown was measured by flow cytometry, with statistical analysis on the right. F : Several cell clones formed before and after SMS knockdown, with statistical results. G : Migration of cells before and after SMS knockdown measured by scratch wound healing assay. H : Migration and invasive ability of cells before and after SMS knockdown measured by transwell assay. NC : normal control. *** p <0.001.
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    Association between EIF4E and PBK and patient survival. The co-expression relationship between EIF4E and PBK was performed by the cBioPortal online platform (A). The expression of EIF4E was downregulated after PBK knockdown in <t>HS683</t> (B). GEPIA online platform was used to perform the expression of EIF4E in glioma and its effect on survival. EIF4E mRNA expression in GBM compared to normal brain tissues (C) in which normal samples are marked in gray and GBM samples are marked in red. High EIF4E expression decreases both overall survival (D) and disease-free survival (E).
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    hs683 cell line  (Procell Inc)
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    Association between EIF4E and PBK and patient survival. The co-expression relationship between EIF4E and PBK was performed by the cBioPortal online platform (A). The expression of EIF4E was downregulated after PBK knockdown in <t>HS683</t> (B). GEPIA online platform was used to perform the expression of EIF4E in glioma and its effect on survival. EIF4E mRNA expression in GBM compared to normal brain tissues (C) in which normal samples are marked in gray and GBM samples are marked in red. High EIF4E expression decreases both overall survival (D) and disease-free survival (E).
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    Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in HS683 and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Journal: Molecular Therapy Oncology

    Article Title: Integrating multi-omics analysis and machine learning to refine molecular subtypes and prognostic assessment of lower-grade glioma

    doi: 10.1016/j.omton.2026.201227

    Figure Lengend Snippet: Expression and functional validation of CSDC2 in LGG (A–C) IHC and WB were used to evaluate the expression of CSDC2 in LGG and adjacent tissue. (D and E) Western blot analysis confirming knockdown of CSDC2 in HS683 and H4 cells. (F and G, and J–M) Effect of CSDC2 knockdown on the migration and invasion of HS683 and H4 cells, as determined by Transwell migration and Matrigel invasion assays. (H, I, N, and O) The effect of CSDC2 knockdown on the migration of HS683 and H4 LGG cells was analyzed by a scratch assay. (P and Q) The effect of CSDC2 knockdown on the proliferation of HS683 and H4 LGG cells was analyzed using a Cell Counting Kit-8. (R) MG-132 and CSDC2 were assembled mainly by hydrogen bonding. (S and T) The effect of MG-132 on the proliferation of HS683 and H4 LGG cells was analyzed by a Cell Counting Kit-8. (U and V) The effect of MG-132 on the apoptosis of HS683 and H4 LGG cells was analyzed by flow cytometry. Scale bars=100 μm. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Article Snippet: The human LGG cell lines HS683 and H4, as well as the human embryonic kidney cell line HEK293T, were purchased from the American Type Culture Collection (ATCC).

    Techniques: Expressing, Functional Assay, Biomarker Discovery, Western Blot, Knockdown, Migration, Wound Healing Assay, Cell Counting, Flow Cytometry

    Knockdown of spermine synthase (SMS) inhibits the malignant behavior of lower-grade glioma cells. A : Western blot analysis of SMS protein expression levels in human normal glial cell lines CP-H121/122/123 and glioma cell line HS683/U251, with statistical analysis on the right. B : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by Western blot, with protein expression analysis on the right. C : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by quantitative reverse transcription polymerase chain reaction. D : Cell proliferation before and after SMS knockdown was measured by CCK-8 assay. E : The apoptosis ratio of cells before and after SMS knockdown was measured by flow cytometry, with statistical analysis on the right. F : Several cell clones formed before and after SMS knockdown, with statistical results. G : Migration of cells before and after SMS knockdown measured by scratch wound healing assay. H : Migration and invasive ability of cells before and after SMS knockdown measured by transwell assay. NC : normal control. *** p <0.001.

    Journal: Journal of Korean Neurosurgical Society

    Article Title: Spermine Synthase : A Potential Prognostic Marker for Lower-Grade Gliomas

    doi: 10.3340/jkns.2024.0080

    Figure Lengend Snippet: Knockdown of spermine synthase (SMS) inhibits the malignant behavior of lower-grade glioma cells. A : Western blot analysis of SMS protein expression levels in human normal glial cell lines CP-H121/122/123 and glioma cell line HS683/U251, with statistical analysis on the right. B : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by Western blot, with protein expression analysis on the right. C : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by quantitative reverse transcription polymerase chain reaction. D : Cell proliferation before and after SMS knockdown was measured by CCK-8 assay. E : The apoptosis ratio of cells before and after SMS knockdown was measured by flow cytometry, with statistical analysis on the right. F : Several cell clones formed before and after SMS knockdown, with statistical results. G : Migration of cells before and after SMS knockdown measured by scratch wound healing assay. H : Migration and invasive ability of cells before and after SMS knockdown measured by transwell assay. NC : normal control. *** p <0.001.

    Article Snippet: The HS683 (HTB-138) human oligodendroglioma cell line and the U251 (HTB-17) glioblastoma cell line were obtained from ATCC (Manassas, VA, USA).

    Techniques: Knockdown, Western Blot, Expressing, Reverse Transcription, Polymerase Chain Reaction, CCK-8 Assay, Flow Cytometry, Clone Assay, Migration, Wound Healing Assay, Transwell Assay, Control

    Association between EIF4E and PBK and patient survival. The co-expression relationship between EIF4E and PBK was performed by the cBioPortal online platform (A). The expression of EIF4E was downregulated after PBK knockdown in HS683 (B). GEPIA online platform was used to perform the expression of EIF4E in glioma and its effect on survival. EIF4E mRNA expression in GBM compared to normal brain tissues (C) in which normal samples are marked in gray and GBM samples are marked in red. High EIF4E expression decreases both overall survival (D) and disease-free survival (E).

    Journal: Annals of Medicine and Surgery

    Article Title: Unraveling the role of PBK in glioblastoma: from molecular mechanisms to therapeutic targets

    doi: 10.1097/MS9.0000000000002708

    Figure Lengend Snippet: Association between EIF4E and PBK and patient survival. The co-expression relationship between EIF4E and PBK was performed by the cBioPortal online platform (A). The expression of EIF4E was downregulated after PBK knockdown in HS683 (B). GEPIA online platform was used to perform the expression of EIF4E in glioma and its effect on survival. EIF4E mRNA expression in GBM compared to normal brain tissues (C) in which normal samples are marked in gray and GBM samples are marked in red. High EIF4E expression decreases both overall survival (D) and disease-free survival (E).

    Article Snippet: The human glioma cell line HS683 was obtained from the American Type Culture Collection (ATCC) and cultured according to ATCC instructions in DMEM medium with 10% FBS in a 5% CO 2 incubator at 37°C. shRNA sequences for PBK knockdown and cell line establishment were based on a previous study .

    Techniques: Expressing, Knockdown