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viral construct  (Addgene inc)


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    Structured Review

    Addgene inc viral construct
    Viral Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 739 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hm3d+mcherry/pAAV-hSyn-DIO-hM3D(Gq)-mCherry+(Plasmid+%2344361)/pm42830177-55-3-6
    Average 96 stars, based on 739 article reviews
    viral construct - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons
    Article Snippet: .. Viral vector microinjection We used 2 viral constructs, AAV8-hSyn-DIO-hM3Dq-mCherry and AAV8-hSyn-DIO-HA-KORD-IRES-mCitrine (#44361 and #65417, titer ≥ 4×10 12 vg/mL; University of North Carolina Vector Core, Chapel Hill, NC and Addgene, Cambridge, MA), to induce Cre-dependent expression of hM3D-mCherry and KORD-IRES-mCitrine in spinal dorsal horn neurons of VGluT2-Cre mice. ..

    Article Title: Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequences from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned them into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene # 83895) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Article Title: Neuromodulation-induced burst firing in parvalbumin interneurons of the basolateral amygdala mediates transition between fear-associated network and behavioral states
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequence from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) ( ) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned it into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene #83895) ( ) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Microinjection:

    Article Title: Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons
    Article Snippet: .. Viral vector microinjection We used 2 viral constructs, AAV8-hSyn-DIO-hM3Dq-mCherry and AAV8-hSyn-DIO-HA-KORD-IRES-mCitrine (#44361 and #65417, titer ≥ 4×10 12 vg/mL; University of North Carolina Vector Core, Chapel Hill, NC and Addgene, Cambridge, MA), to induce Cre-dependent expression of hM3D-mCherry and KORD-IRES-mCitrine in spinal dorsal horn neurons of VGluT2-Cre mice. ..

    Construct:

    Article Title: Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons
    Article Snippet: .. Viral vector microinjection We used 2 viral constructs, AAV8-hSyn-DIO-hM3Dq-mCherry and AAV8-hSyn-DIO-HA-KORD-IRES-mCitrine (#44361 and #65417, titer ≥ 4×10 12 vg/mL; University of North Carolina Vector Core, Chapel Hill, NC and Addgene, Cambridge, MA), to induce Cre-dependent expression of hM3D-mCherry and KORD-IRES-mCitrine in spinal dorsal horn neurons of VGluT2-Cre mice. ..

    Article Title: Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons
    Article Snippet: .. We used 2 viral constructs, AAV8-hSyn-DIO-hM3Dq-mCherry and AAV8-hSyn-DIO-HA-KORD-IRES-mCitrine (#44361 and #65417, titer ≥ 4×10 12 vg/mL; University of North Carolina Vector Core, Chapel Hill, NC and Addgene, Cambridge, MA), to induce Cre-dependent expression of hM3D-mCherry and KORD-IRES-mCitrine in spinal dorsal horn neurons of VGluT2-Cre mice. ..

    Expressing:

    Article Title: Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons
    Article Snippet: .. Viral vector microinjection We used 2 viral constructs, AAV8-hSyn-DIO-hM3Dq-mCherry and AAV8-hSyn-DIO-HA-KORD-IRES-mCitrine (#44361 and #65417, titer ≥ 4×10 12 vg/mL; University of North Carolina Vector Core, Chapel Hill, NC and Addgene, Cambridge, MA), to induce Cre-dependent expression of hM3D-mCherry and KORD-IRES-mCitrine in spinal dorsal horn neurons of VGluT2-Cre mice. ..

    Article Title: Regulating nociceptive transmission by VGluT2-expressing spinal dorsal horn neurons
    Article Snippet: .. We used 2 viral constructs, AAV8-hSyn-DIO-hM3Dq-mCherry and AAV8-hSyn-DIO-HA-KORD-IRES-mCitrine (#44361 and #65417, titer ≥ 4×10 12 vg/mL; University of North Carolina Vector Core, Chapel Hill, NC and Addgene, Cambridge, MA), to induce Cre-dependent expression of hM3D-mCherry and KORD-IRES-mCitrine in spinal dorsal horn neurons of VGluT2-Cre mice. ..

    Cloning:

    Article Title: Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequences from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned them into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene # 83895) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Article Title: Neuromodulation-induced burst firing in parvalbumin interneurons of the basolateral amygdala mediates transition between fear-associated network and behavioral states
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequence from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) ( ) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned it into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene #83895) ( ) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Bioprocessing:

    Article Title: Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequences from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned them into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene # 83895) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Article Title: Neuromodulation-induced burst firing in parvalbumin interneurons of the basolateral amygdala mediates transition between fear-associated network and behavioral states
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequence from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) ( ) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned it into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene #83895) ( ) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Virus:

    Article Title: Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequences from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned them into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene # 83895) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Article Title: Neuromodulation-induced burst firing in parvalbumin interneurons of the basolateral amygdala mediates transition between fear-associated network and behavioral states
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequence from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) ( ) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned it into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene #83895) ( ) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Amplification:

    Article Title: Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequences from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned them into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene # 83895) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Article Title: Neuromodulation-induced burst firing in parvalbumin interneurons of the basolateral amygdala mediates transition between fear-associated network and behavioral states
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequence from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) ( ) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned it into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene #83895) ( ) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Clone Assay:

    Article Title: Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequences from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned them into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene # 83895) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Article Title: Neuromodulation-induced burst firing in parvalbumin interneurons of the basolateral amygdala mediates transition between fear-associated network and behavioral states
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequence from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) ( ) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned it into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene #83895) ( ) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.

    Sequencing:

    Article Title: Neuromodulation-induced burst firing in parvalbumin interneurons of the basolateral amygdala mediates transition between fear-associated network and behavioral states
    Article Snippet: .. For cloning of Cre-dependent hDlx AAV virus, we amplified the hM3D-mCherry and mCherry coding sequence from the plasmid pAAV-hSyn-DIO-hM3D(Gq)-mCherry (a gift from Bryan Roth, Addgene # 44361) ( ) and pAAV-hSyn-DIO-mCherry (also a gift from Bryan Roth, Addgene # 50459) and cloned it into a pAAV-hDlx-Flex-GFP vector backbone (a gift from Gordon Fishell, Addgene #83895) ( ) at AccI and NheI cloning sites. .. The coding sequence of adra1A was synthesized from Bio Basic Inc. and cloned into a pAAV-hDlx-Flex backbone.



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    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, <t>and</t> <t>the</t> <t>AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA</t> was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.
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    Image Search Results


    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.

    Journal: The Journal of Experimental Medicine

    Article Title: Central neurons encode interleukin-1β signals and mediate stress-induced inflammation

    doi: 10.1084/jem.20252000

    Figure Lengend Snippet: BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.

    Article Snippet: For chemogenetic studies, either AAV-hSyn-DIO-hM3D(Gq)–mCherry (cat #44361; Addgene) or AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (cat#154868; Addgene) was used.

    Techniques: Anterograde Tracing, Injection, Expressing, Saline, Control