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hek lucia rig  (InvivoGen)


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    Structured Review

    InvivoGen hek lucia rig
    Concentration-dependent receptor agonist activities of conjugated NOD1/PRR agonists compared to bona fide commercially available ligands of corresponding PRRs. SEAP activities were measured in HEK-Blue hTLR4, HEK-Blue hTLR7 <t>and</t> <t>HEK-Lucia</t> <t>RIG-I</t> cell supernatants after incubation for 18 h with compounds of interest at different concentration ranges. The data are shown as fold increases of NF-κB transcriptional/luciferase activity relative to the negative control (0.1% DMSO) and are expressed as means ± SEM of at least three independent experiments.
    Hek Lucia Rig, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hkl+hrigi/HEK-Lucia+RIG-I+Cells/pmc13495014-205-0-6
    Average 94 stars, based on 22 article reviews
    hek lucia rig - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Conjugated NOD1-Centered Dual Agonists Exhibit Modest In Vitro Immunomodulation but Potent In Vivo Adjuvanticity"

    Article Title: Conjugated NOD1-Centered Dual Agonists Exhibit Modest In Vitro Immunomodulation but Potent In Vivo Adjuvanticity

    Journal: ACS Bio & Med Chem Au

    doi: 10.1021/acsbiomedchemau.6c00080

    Concentration-dependent receptor agonist activities of conjugated NOD1/PRR agonists compared to bona fide commercially available ligands of corresponding PRRs. SEAP activities were measured in HEK-Blue hTLR4, HEK-Blue hTLR7 and HEK-Lucia RIG-I cell supernatants after incubation for 18 h with compounds of interest at different concentration ranges. The data are shown as fold increases of NF-κB transcriptional/luciferase activity relative to the negative control (0.1% DMSO) and are expressed as means ± SEM of at least three independent experiments.
    Figure Legend Snippet: Concentration-dependent receptor agonist activities of conjugated NOD1/PRR agonists compared to bona fide commercially available ligands of corresponding PRRs. SEAP activities were measured in HEK-Blue hTLR4, HEK-Blue hTLR7 and HEK-Lucia RIG-I cell supernatants after incubation for 18 h with compounds of interest at different concentration ranges. The data are shown as fold increases of NF-κB transcriptional/luciferase activity relative to the negative control (0.1% DMSO) and are expressed as means ± SEM of at least three independent experiments.

    Techniques Used: Concentration Assay, Incubation, Luciferase, Activity Assay, Negative Control

    Related Articles

    Luciferase:

    Article Title: The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor
    Article Snippet: The pcDNA TM 3.1(+) empty vector (Life Technologies) was used as a control for cDNA transfection. .. HEK-293T cells (cat. number 103, NIH AIDS Research and Reference Reagent Program) and Lucia luciferase reporter HEK-293 cells expressing human RIG-I, HEK-Lucia RIG-I, (hkl-hrigi, InvivoGen, San Diego, CA, USA) were grown at 37 °C in a humidified atmosphere with 5% CO 2 in DMEM (Lonza, Verviers, Belgium) supplemented with 10% foetal calf serum (FCS) (Lonza), 1% L-glutamine and 1% penicillin–streptomycin (Lonza, Basel, Switzerland). .. The cells were harvested and passaged every 3 days using trypsin-EDTA (L0930-100) (Biowest, Lakewood Ranch, FL, USA) or Versene 1× (15040-066; Gibco Chemicals, Thermo Fisher Scientific, MA, USA).

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. African green monkey kidney cells (Vero; ATCC, CCL-81) were used in focus-forming assays to quantify Zika virus (ZIKV) viral loads in mouse serum.

    Expressing:

    Article Title: The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor
    Article Snippet: The pcDNA TM 3.1(+) empty vector (Life Technologies) was used as a control for cDNA transfection. .. HEK-293T cells (cat. number 103, NIH AIDS Research and Reference Reagent Program) and Lucia luciferase reporter HEK-293 cells expressing human RIG-I, HEK-Lucia RIG-I, (hkl-hrigi, InvivoGen, San Diego, CA, USA) were grown at 37 °C in a humidified atmosphere with 5% CO 2 in DMEM (Lonza, Verviers, Belgium) supplemented with 10% foetal calf serum (FCS) (Lonza), 1% L-glutamine and 1% penicillin–streptomycin (Lonza, Basel, Switzerland). .. The cells were harvested and passaged every 3 days using trypsin-EDTA (L0930-100) (Biowest, Lakewood Ranch, FL, USA) or Versene 1× (15040-066; Gibco Chemicals, Thermo Fisher Scientific, MA, USA).

    Activity Assay:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. African green monkey kidney cells (Vero; ATCC, CCL-81) were used in focus-forming assays to quantify Zika virus (ZIKV) viral loads in mouse serum.

    Transfection:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. African green monkey kidney cells (Vero; ATCC, CCL-81) were used in focus-forming assays to quantify Zika virus (ZIKV) viral loads in mouse serum.

    Activation Assay:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. African green monkey kidney cells (Vero; ATCC, CCL-81) were used in focus-forming assays to quantify Zika virus (ZIKV) viral loads in mouse serum.

    other:

    Article Title: Non‐Targeting shRNA‐Encoded Plasmid DNA Enhances Protective Immunity Through RIDD‐RIG‐I Signaling Pathway in the Zika Virus Animal Model
    Article Snippet: The RIG‐I reporter cell line, HEK‐Lucia RIG‐I (InvivoGen; Cat. # hkl‐hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG‐I pathway activation.

    Derivative Assay:

    Article Title: Modulation of SF3B1 in the pre-mRNA spliceosome induces a RIG-I-dependent type I IFN response
    Article Snippet: B16F10, CT26, 4T1, and MC38 murine lines were purchased from American Type Culture Collection, expanded, confirmed to be mycoplasma free, and then frozen as aliquots. .. HEK reporter lines, A549-Dual, and the derived genetic KO reporter lines were purchased from Invivogen: HEK-Lucia Null (hkl-null), HEK-Lucia RIG-I (hkl-hrigi), A549-Dual (a549d-nfis), A549-Dual-RIG-I-KO (a549d-korigi), A549-Dual-MDA5-KO (a549d-komda5), A549-Dual-MAVS-KO (a549d-komavs), THP1-Dual (thpd-nfis), THP1-Dual-IRF3-KO (thpd-koirf3), and THP1-Dual-MAVS-KO (thpd-komavs). .. All reporter cell lines from Invivogen were maintained as recommended by the manufacturer.



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    Concentration-dependent receptor agonist activities of conjugated NOD1/PRR agonists compared to bona fide commercially available ligands of corresponding PRRs. SEAP activities were measured in HEK-Blue hTLR4, HEK-Blue hTLR7 <t>and</t> <t>HEK-Lucia</t> <t>RIG-I</t> cell supernatants after incubation for 18 h with compounds of interest at different concentration ranges. The data are shown as fold increases of NF-κB transcriptional/luciferase activity relative to the negative control (0.1% DMSO) and are expressed as means ± SEM of at least three independent experiments.
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    Image Search Results


    Concentration-dependent receptor agonist activities of conjugated NOD1/PRR agonists compared to bona fide commercially available ligands of corresponding PRRs. SEAP activities were measured in HEK-Blue hTLR4, HEK-Blue hTLR7 and HEK-Lucia RIG-I cell supernatants after incubation for 18 h with compounds of interest at different concentration ranges. The data are shown as fold increases of NF-κB transcriptional/luciferase activity relative to the negative control (0.1% DMSO) and are expressed as means ± SEM of at least three independent experiments.

    Journal: ACS Bio & Med Chem Au

    Article Title: Conjugated NOD1-Centered Dual Agonists Exhibit Modest In Vitro Immunomodulation but Potent In Vivo Adjuvanticity

    doi: 10.1021/acsbiomedchemau.6c00080

    Figure Lengend Snippet: Concentration-dependent receptor agonist activities of conjugated NOD1/PRR agonists compared to bona fide commercially available ligands of corresponding PRRs. SEAP activities were measured in HEK-Blue hTLR4, HEK-Blue hTLR7 and HEK-Lucia RIG-I cell supernatants after incubation for 18 h with compounds of interest at different concentration ranges. The data are shown as fold increases of NF-κB transcriptional/luciferase activity relative to the negative control (0.1% DMSO) and are expressed as means ± SEM of at least three independent experiments.

    Article Snippet: HEK-Lucia RIG-I cells (Cat. code: hkl-hrigi, Invivogen, San Diego, CA) are derived from HEK293 cells by cotransfection of hRIG-I gene and an IFN-inducible Lucia luciferase reporter gene.

    Techniques: Concentration Assay, Incubation, Luciferase, Activity Assay, Negative Control