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high throughput microarray platform illumina 27k array  (Illumina Inc)


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    Structured Review

    Illumina Inc high throughput microarray platform illumina 27k array
    High Throughput Microarray Platform Illumina 27k Array, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high-throughput+microarray+platform/27k+array/pmc03763832-98-2-6
    Average 90 stars, based on 1 article reviews
    high throughput microarray platform illumina 27k array - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: qPCR-based methods for expression analysis of miRNAs.
    Article Snippet: A study reported the comparison of the results generated from the microarray platforms (Affymetrix, Illumina and Agilent), ultra-high-throughput sequencing (Illumina platform) and qPCR (TaqMan Human MicroRNA A Array v2.0 from Applied Biosystems) for miRNA levels [71].

    Article Title: Genome-Wide Mapping of Collier In Vivo Binding Sites Highlights Its Hierarchical Position in Different Transcription Regulatory Networks
    Article Snippet: DNA from two independent Col and Mock iPs was pooled before ChiP fragment amplification and High-throughput sequencing (Genome analyzer IIx, Illumina; Microarray and sequencing platform, IGBMC, Illkirch).

    Article Title: MicroRNA Signatures in the Upper Urinary Tract Urothelial Carcinoma Scenario: Ready for the Game Changer?
    Article Snippet: [ ] , ↑ miR-664a-3p ↑ miR-431-5p ↑ miR-423-5p ↑ miR-191-5p ↑ miR-33b-3p ↑ miR-26a-5p ↑ miR-22-3p ↑ miR-16-5p ↑ let-7b-5p ↑ let-7c (UTUC patients vs. controls) , Serum samples , Blood drawn within 1 day after admission, the coagulated blood samples were collected in tubes containing a separating gel and clot activator, then centrifugated at 1500× g for 15 min at 4 °C. The supernatant was centrifugated at 1500× g for 15 min and stored at −80 °C. , Retrospective, monocenter , 58 UTUC patients and 42 cancer-free controls with non-neoplastic hematuria (12 UTUC pts and 12 controls in the training set, 46 UTUC pts and 30 controls in the validation set) , DC. UTUC: Age: 28 <68 y, 30 ≥68 y; Gender: 17 female; Stage: 21 I stage, 14 II stage, 20 III stage, 3 stage IV; Grade: 1 papillary urothelial neoplasia of low malignant potential, 17 low, 40 high. Normal: Age: 22 <60 y, 20 ≥68 y; Gender: 12 female. , RNA extracted with miRNeasy Serum/Plasma kit (Qiagen); miRNAs expression profiling: deep sequencing platform Illumina HiSeqTM 2000; RT-qPCR Validation: All-in-One miRNA RT-qPCR Detection kit (GeneCapoiea-FulenGen). qPCR with SYBR green on Applied Biosystems Step One Plus System (Applied Biosystems). RNU6-2 as endogenous reference gene for normalization. , Diagnosis. UTUC patients vs. controls, AUC > 0.8.

    Article Title: Transcriptomic and Cellular Content Analysis of Colorectal Cancer by Combining Multiple Independent Cohorts
    Article Snippet: By this filtering, we selected the high-throughput sequencing platform GPL16791 (Illumina HiSeq 2500) and the most abundant microarray platform GPL570 (Affymetrix Human Genome U133 Plus 2.0 Array).

    High Throughput Screening Assay:

    Article Title: Pharmacogenomic Discovery Delineating the Genetic Basis of Drug Response
    Article Snippet: .. Recently, a high throughput microarray platform (Illumina 27K array, covering ~27,000 promoter CpGs), has been used for investigating DNA methylation variation in promoter regions in the CEU [ 24 , 25 ] and YRI [ 24 ] samples. .. More recnetly, a much more comprehnsive dataset ( ) uisng the Illumina 450k array, covering more than 450,000 CpGs across the human genome [ 48 ], has been generated for the HapMap CEU and YRI samples [ 23 , 49 ], thus allowing epigenomic mapping in pharmacogenomic discovery to incorporate this important gene regulatory mechanism.

    Article Title: Maturation of the human dorsolateral prefrontal cortex coincides with a dynamic shift in microRNA expression.
    Article Snippet: RNA concentration and integrity was determined using the Bioanalyzer 2100 electrophoresis system (Agilent Technologies), where the mean RIN was 6.9. .. To eliminate any quality bias issues, all dissection, extraction, and quality control protocols were standardized and constant for both sets of tissue. miRNA Microarray Profiling of miRNA expression was achieved using a high throughput commercial bead-based miRNA microarray platform (Illumina). ..

    Article Title: Human Regulatory T Cells Mediate Transcriptional Modulation of Dendritic Cell Function.
    Article Snippet: The RNA integrity was confirmed using an Agilent 2100 bioanalyzer (Agilent, U.K.). .. To control for any changes in gene expression caused by shear forces, all samples were subjected to a FACS sorting procedure prior to RNA extraction. mRNA profiling was carried out on the Illumina HT12 microarray platform by the High-Throughput Genomics group at theWellcome Trust Centre for Human Genetics, as per the manufacturer’s protocol (GEO accession number GSE72893, http://www.ncbi.nlm.nih.gov/geo/). ..

    Microarray:

    Article Title: Pharmacogenomic Discovery Delineating the Genetic Basis of Drug Response
    Article Snippet: .. Recently, a high throughput microarray platform (Illumina 27K array, covering ~27,000 promoter CpGs), has been used for investigating DNA methylation variation in promoter regions in the CEU [ 24 , 25 ] and YRI [ 24 ] samples. .. More recnetly, a much more comprehnsive dataset ( ) uisng the Illumina 450k array, covering more than 450,000 CpGs across the human genome [ 48 ], has been generated for the HapMap CEU and YRI samples [ 23 , 49 ], thus allowing epigenomic mapping in pharmacogenomic discovery to incorporate this important gene regulatory mechanism.

    Article Title: Maturation of the human dorsolateral prefrontal cortex coincides with a dynamic shift in microRNA expression.
    Article Snippet: RNA concentration and integrity was determined using the Bioanalyzer 2100 electrophoresis system (Agilent Technologies), where the mean RIN was 6.9. .. To eliminate any quality bias issues, all dissection, extraction, and quality control protocols were standardized and constant for both sets of tissue. miRNA Microarray Profiling of miRNA expression was achieved using a high throughput commercial bead-based miRNA microarray platform (Illumina). ..

    Article Title: Human Regulatory T Cells Mediate Transcriptional Modulation of Dendritic Cell Function.
    Article Snippet: The RNA integrity was confirmed using an Agilent 2100 bioanalyzer (Agilent, U.K.). .. To control for any changes in gene expression caused by shear forces, all samples were subjected to a FACS sorting procedure prior to RNA extraction. mRNA profiling was carried out on the Illumina HT12 microarray platform by the High-Throughput Genomics group at theWellcome Trust Centre for Human Genetics, as per the manufacturer’s protocol (GEO accession number GSE72893, http://www.ncbi.nlm.nih.gov/geo/). ..

    Article Title: Meta-gene markers predict meningioma recurrence with high accuracy
    Article Snippet: .. Gene expression analysis for these studies was carried out using microarray platforms Affymetrix Human Genome U133A, Affymetrix Human Genome U133 Plus 2.0, Illumina Human HT-12 V4.0, SU Homo Sapiens 912 (a custom-built chip with 912 cancer-related genes), and the high throughput sequencing platform Illumina HiSeq 4000, respectively. ..

    DNA Methylation Assay:

    Article Title: Pharmacogenomic Discovery Delineating the Genetic Basis of Drug Response
    Article Snippet: .. Recently, a high throughput microarray platform (Illumina 27K array, covering ~27,000 promoter CpGs), has been used for investigating DNA methylation variation in promoter regions in the CEU [ 24 , 25 ] and YRI [ 24 ] samples. .. More recnetly, a much more comprehnsive dataset ( ) uisng the Illumina 450k array, covering more than 450,000 CpGs across the human genome [ 48 ], has been generated for the HapMap CEU and YRI samples [ 23 , 49 ], thus allowing epigenomic mapping in pharmacogenomic discovery to incorporate this important gene regulatory mechanism.

    Dissection:

    Article Title: Maturation of the human dorsolateral prefrontal cortex coincides with a dynamic shift in microRNA expression.
    Article Snippet: RNA concentration and integrity was determined using the Bioanalyzer 2100 electrophoresis system (Agilent Technologies), where the mean RIN was 6.9. .. To eliminate any quality bias issues, all dissection, extraction, and quality control protocols were standardized and constant for both sets of tissue. miRNA Microarray Profiling of miRNA expression was achieved using a high throughput commercial bead-based miRNA microarray platform (Illumina). ..

    Extraction:

    Article Title: Maturation of the human dorsolateral prefrontal cortex coincides with a dynamic shift in microRNA expression.
    Article Snippet: RNA concentration and integrity was determined using the Bioanalyzer 2100 electrophoresis system (Agilent Technologies), where the mean RIN was 6.9. .. To eliminate any quality bias issues, all dissection, extraction, and quality control protocols were standardized and constant for both sets of tissue. miRNA Microarray Profiling of miRNA expression was achieved using a high throughput commercial bead-based miRNA microarray platform (Illumina). ..

    Control:

    Article Title: Maturation of the human dorsolateral prefrontal cortex coincides with a dynamic shift in microRNA expression.
    Article Snippet: RNA concentration and integrity was determined using the Bioanalyzer 2100 electrophoresis system (Agilent Technologies), where the mean RIN was 6.9. .. To eliminate any quality bias issues, all dissection, extraction, and quality control protocols were standardized and constant for both sets of tissue. miRNA Microarray Profiling of miRNA expression was achieved using a high throughput commercial bead-based miRNA microarray platform (Illumina). ..

    Article Title: Human Regulatory T Cells Mediate Transcriptional Modulation of Dendritic Cell Function.
    Article Snippet: The RNA integrity was confirmed using an Agilent 2100 bioanalyzer (Agilent, U.K.). .. To control for any changes in gene expression caused by shear forces, all samples were subjected to a FACS sorting procedure prior to RNA extraction. mRNA profiling was carried out on the Illumina HT12 microarray platform by the High-Throughput Genomics group at theWellcome Trust Centre for Human Genetics, as per the manufacturer’s protocol (GEO accession number GSE72893, http://www.ncbi.nlm.nih.gov/geo/). ..

    Expressing:

    Article Title: Maturation of the human dorsolateral prefrontal cortex coincides with a dynamic shift in microRNA expression.
    Article Snippet: RNA concentration and integrity was determined using the Bioanalyzer 2100 electrophoresis system (Agilent Technologies), where the mean RIN was 6.9. .. To eliminate any quality bias issues, all dissection, extraction, and quality control protocols were standardized and constant for both sets of tissue. miRNA Microarray Profiling of miRNA expression was achieved using a high throughput commercial bead-based miRNA microarray platform (Illumina). ..

    Gene Expression:

    Article Title: Human Regulatory T Cells Mediate Transcriptional Modulation of Dendritic Cell Function.
    Article Snippet: The RNA integrity was confirmed using an Agilent 2100 bioanalyzer (Agilent, U.K.). .. To control for any changes in gene expression caused by shear forces, all samples were subjected to a FACS sorting procedure prior to RNA extraction. mRNA profiling was carried out on the Illumina HT12 microarray platform by the High-Throughput Genomics group at theWellcome Trust Centre for Human Genetics, as per the manufacturer’s protocol (GEO accession number GSE72893, http://www.ncbi.nlm.nih.gov/geo/). ..

    Article Title: Meta-gene markers predict meningioma recurrence with high accuracy
    Article Snippet: .. Gene expression analysis for these studies was carried out using microarray platforms Affymetrix Human Genome U133A, Affymetrix Human Genome U133 Plus 2.0, Illumina Human HT-12 V4.0, SU Homo Sapiens 912 (a custom-built chip with 912 cancer-related genes), and the high throughput sequencing platform Illumina HiSeq 4000, respectively. ..

    Shear:

    Article Title: Human Regulatory T Cells Mediate Transcriptional Modulation of Dendritic Cell Function.
    Article Snippet: The RNA integrity was confirmed using an Agilent 2100 bioanalyzer (Agilent, U.K.). .. To control for any changes in gene expression caused by shear forces, all samples were subjected to a FACS sorting procedure prior to RNA extraction. mRNA profiling was carried out on the Illumina HT12 microarray platform by the High-Throughput Genomics group at theWellcome Trust Centre for Human Genetics, as per the manufacturer’s protocol (GEO accession number GSE72893, http://www.ncbi.nlm.nih.gov/geo/). ..

    FACS:

    Article Title: Human Regulatory T Cells Mediate Transcriptional Modulation of Dendritic Cell Function.
    Article Snippet: The RNA integrity was confirmed using an Agilent 2100 bioanalyzer (Agilent, U.K.). .. To control for any changes in gene expression caused by shear forces, all samples were subjected to a FACS sorting procedure prior to RNA extraction. mRNA profiling was carried out on the Illumina HT12 microarray platform by the High-Throughput Genomics group at theWellcome Trust Centre for Human Genetics, as per the manufacturer’s protocol (GEO accession number GSE72893, http://www.ncbi.nlm.nih.gov/geo/). ..

    RNA Extraction:

    Article Title: Human Regulatory T Cells Mediate Transcriptional Modulation of Dendritic Cell Function.
    Article Snippet: The RNA integrity was confirmed using an Agilent 2100 bioanalyzer (Agilent, U.K.). .. To control for any changes in gene expression caused by shear forces, all samples were subjected to a FACS sorting procedure prior to RNA extraction. mRNA profiling was carried out on the Illumina HT12 microarray platform by the High-Throughput Genomics group at theWellcome Trust Centre for Human Genetics, as per the manufacturer’s protocol (GEO accession number GSE72893, http://www.ncbi.nlm.nih.gov/geo/). ..

    Next-Generation Sequencing:

    Article Title: Meta-gene markers predict meningioma recurrence with high accuracy
    Article Snippet: .. Gene expression analysis for these studies was carried out using microarray platforms Affymetrix Human Genome U133A, Affymetrix Human Genome U133 Plus 2.0, Illumina Human HT-12 V4.0, SU Homo Sapiens 912 (a custom-built chip with 912 cancer-related genes), and the high throughput sequencing platform Illumina HiSeq 4000, respectively. ..



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    Number of present miRNAs at different stages (day 0, day 12, day 19 and day 26) during cardiomyocyte specific differentiation and <t> maturation. </t>
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    Image Search Results


    Number of present miRNAs at different stages (day 0, day 12, day 19 and day 26) during cardiomyocyte specific differentiation and  maturation.

    Journal: PLoS ONE

    Article Title: MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms

    doi: 10.1371/journal.pone.0025809

    Figure Lengend Snippet: Number of present miRNAs at different stages (day 0, day 12, day 19 and day 26) during cardiomyocyte specific differentiation and maturation.

    Article Snippet: In the present manuscript paper, a transgenic mouse ES cell clone was used to generate uniform Cor.At® cardiomyocytes. miRNAs were profiled for undifferentiated transgenic ES cells (day 0) and at time points day 12, day 19 and day 26 during cardiomyocyte-specific differentiation and maturation using 2 high throughput microarray platforms provided from Affymetrix and Febit.

    Techniques:

    The number of up/down regulated miRNAs at different maturation stages (day 12, day 19 and day 26) compared to undifferentiated ES cells were denoted with arrows up/down.

    Journal: PLoS ONE

    Article Title: MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms

    doi: 10.1371/journal.pone.0025809

    Figure Lengend Snippet: The number of up/down regulated miRNAs at different maturation stages (day 12, day 19 and day 26) compared to undifferentiated ES cells were denoted with arrows up/down.

    Article Snippet: In the present manuscript paper, a transgenic mouse ES cell clone was used to generate uniform Cor.At® cardiomyocytes. miRNAs were profiled for undifferentiated transgenic ES cells (day 0) and at time points day 12, day 19 and day 26 during cardiomyocyte-specific differentiation and maturation using 2 high throughput microarray platforms provided from Affymetrix and Febit.

    Techniques: