bio scale 241 mini macro prep high q cartridge column (Bio-Rad)
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bio scale 241 mini macro prep high q cartridge column
Bio Scale 241 Mini Macro Prep High Q Cartridge Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+q+column/Bio-Scale+Mini+Macro-Prep+High+S+Cartridge/10__1016_slash_j__bbrc__2025__152810-107-18-26
Average 93 stars, based on 28 article reviews
Bio Scale 241 Mini Macro Prep High Q Cartridge Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+q+column/Bio-Scale+Mini+Macro-Prep+High+S+Cartridge/10__1016_slash_j__bbrc__2025__152810-107-18-26
Average 93 stars, based on 28 article reviews
bio scale 241 mini macro prep high q cartridge column - by Bioz Stars,
2026-09
93/100 stars
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Sequencing:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Plasmid Preparation:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Lysis:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Saline:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials pACEBac-ADDomer (Basic Protocol 4, step 4) E2EP3 neutralizing epitope peptide (see Fig. 7 and Table 2) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Hi5 insect cells Phosphate-buffered saline (PBS) EDTA-free complete protease inhibitor (Roche, Switzerland) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Purification:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display Article Snippet: .. ADDomer was further purified by ion exchange chromatography using a Article Title: Promoter Recognition by a Complex of Spx and the C-Terminal Domain of the RNA Polymerase α Subunit Article Snippet: RNAP was purified from ORB4028 ( spx :: neo his 10 -rpoC ) as previously described using a Ni-NTA affinity column, a heparin agarose, and a Bio-Rad High Q column , , . .. The self-cleavable intein system (New England Biolabs) was used for overproduction and purification of αCTD and Spx. αCTD (residues 225–314) was overproduced in Escherichia coli BL21/pLysS carrying pSN37 and purified using a chitin column and a Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) In Silico:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Software:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Sterility:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Membrane:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Virus:Article Title: VLP‐factory™ and ADDomer © : Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display Article Snippet: Quality control of the MultiBac‐produced ADDomer © sample evidenced negligible nucleic acid or endotoxin contamination (Vragniau et al., ). .. Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table ) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Bam HI and Hin dIII restriction enzymes Hi5 insect cells (ECACC 94‐20‐1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see ) Phosphate‐buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see ) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6‐8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Protease Inhibitor:Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials pACEBac-ADDomer (Basic Protocol 4, step 4) E2EP3 neutralizing epitope peptide (see Fig. 7 and Table 2) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) Hi5 insect cells Phosphate-buffered saline (PBS) EDTA-free complete protease inhibitor (Roche, Switzerland) Ion Exchange Chromatography:Article Title: Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display Article Snippet: .. ADDomer was further purified by ion exchange chromatography using a Article Title: Identification and characterization of an Arabidopsis phosphate starvation-induced secreted acid phosphatase as a vegetative storage protein. Article Snippet: Induction and secretion of acid phosphatases is an adaptive response of plants to phosphate starvation.. The secreted acid phosphatases are believed to scavenge phosphate from organophosphate compounds in the rhizosphere, thereby increasing phosphate availability for plant absorption.. To date, however, all of the characterized phosphate starvation-induced secreted acid phosphatases in plants belong to a unique acid phosphatases subfamily, called purple acid phosphatase. SDS Page:Article Title: VLP-factory™ and ADDomer © : Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display. Article Snippet: .. Materials Human Ad3 gene sequence encoding the penton base protein (GenBank Z29487) Plasmid pACEBac1 (Geneva Biotech SARL, Switzerland; see Table 1) DH10EMBacY cells (Geneva Biotech SARL, Switzerland) BamHI and HindIII restriction enzymes Hi5 insect cells (ECACC 94-20-1) growing in culture SF900 II SFM (Thermo Fisher) to grow insect cells (cat. no. 10902088) Bovine serum albumin (BSA; New England Biolabs, cat. no. B9200) Dimethylsulfoxide (DMSO) Liquid nitrogen Hypotonic lysis buffer (see recipe) Phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, cat. no. 10010023) 15% and 40% (w/v) sucrose Purification buffer gradient solutions (see recipe) In silico design software (ApE, SnapGene, Vector NTI) Centrifuge Gradient Master 107 (BioComp Instruments) Ultracentrifuge with Beckman SW28 rotor Sterile ultracentrifugation tubes (Beckman) 6-8 kDa MWCO dialysis membrane (SpectrumTM Spectra/PorTM; cat. no. 132660) Concentration Assay:Article Title: Rrm3 and Pif1 division of labor during replication through leading and lagging strand G-quadruplex Article Snippet: The supernatant was then loaded on a HisPur Ni-NTA superflow agarose (Thermo Scientific), washed with 20 mM imidazole in buffer L and eluted with 300 mM imidazole in Buffer L. The fractions containing Rrm3 were dialyzed in Buffer P (50 mM Tris–HCl pH 7.5 (4°C), 1 mM EDTA, 1 mM DTT, 20% v/v glycerol and 0.1 mM PMSF) + 400 mM NaCl. .. After extensive dialysis, the sample was diluted 2-fold with buffer P (to reduce the NaCl concentration to 200 mM), loaded on a Article Title: Rrm3 and Pif1 division of labor during replication through leading and lagging strand G-quadruplex. Article Snippet: The supernatant was then loaded on a HisPur Ni-NTA superflow agarose (Thermo Scientific), washed with 20 mM imidazole in buffer L and eluted with 300 mM imidazole in Buffer L. The fractions containing Rrm3 were dialyzed in Buffer P (50 mM Tris–HCl pH 7.5 (4 ◦C), 1 mM EDTA, 1 mM DTT, 20% v / v glycerol and 0.1 mM PMSF) + 400 mM NaCl. .. After extensive dialysis, the sample was diluted 2-fold with buffer P (to reduce the NaCl concentration to 200 mM), loaded on a |