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high binding clear bottom plates  (Greiner Bio)


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    Structured Review

    Greiner Bio high binding clear bottom plates
    High Binding Clear Bottom Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 838 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+384+Well+Ps+F-Bottom/pm41922952-83-2-6
    Average 97 stars, based on 838 article reviews
    high binding clear bottom plates - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Binding Assay:

    Article Title: RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells
    Article Snippet: Following three 10-minute washes in TBS + 0.1% Tween-20, the blot was developed using 1-Step NPT/BCIP (Thermo Fisher) substrate and visualized on an AlphaImager HP (Protein Simple). .. Soluble antigen was diluted to 2 μg/mL in Carbonate Coating Buffer (0.2 M Carbonate-Bicarbonate, pH 9.4 (Thermo Fisher)) and 20 μL was dispensed into the wells of a 384-well High Binding microplate (Greiner). ..

    Article Title: Structure-Based Exploration of Selectivity for ATM Inhibitors in Huntington's Disease.
    Article Snippet: Our group has recently shown that brain-penetrant ataxia telangiectasia-mutated (ATM) kinase inhibitors may have potential as novel therapeutics for the treatment of Huntington’s disease (HD).. However, the previously described pyranonethioxanthenes (e.g., 4) failed to afford selectivity over a vacuolar protein sorting 34 (Vps34) kinase, an important kinase involved with autophagy.. Given that impaired autophagy has been proposed as a pathogenic mechanism of neurodegenerative diseases such as HD, achieving selectivity over Vps34 became an important objective for our program.

    Article Title: A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection.
    Article Snippet: ACE2 binding to RBD analyzed by titration ELISA ACE2 binding to the produced RBD variant antigens was analyzed by ELISA. .. Briefly, 0.1 ug of RBD per well was immobilized on a high binding Microplate (Greiner Bio-One GmbH, Austria.) and incubated ONat 4 °C. ..

    Article Title: ATM kinase inhibitors and compositions and methods of use thereof
    Article Snippet: Reactions contained 0.75 nM ATM, 25 nM full length myc tagged p53 (Eurofins 23-034), 10 μM UltraPure ATP (Promega V915B) and serial dilutions of inhibitors (0.1% DMSO final) in 25 mM HEPES, pH 7.5. .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, high binding microplate (Greiner Bio One 781061), precoated with anti-myc capture antibody overnight (Millipore 05-724) diluted in 1:1000 in PBS, then blocked with Odyssey blocking buffer (LI-COR Biosciences 92740000) for 60 min at 20° C. The terminated reaction was allowed to capture for 2 h at 20° C. Phosphorylation was measured using an antibody specific to p53 S15 phosphorylation (1:5000 Abcam ab38497), anti-rabbit HRP (1:1000 Cell Signalling Technology, 7074) and TMB (ab171522). .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, high binding microplate (Greiner Bio One 781061), precoated with anti-myc capture antibody overnight (Millipore 05-724) diluted in 1:1000 in PBS, then blocked with Odyssey blocking buffer (LI-COR Biosciences 92740000) for 60 min at 20° C. The terminated reaction was allowed to capture for 2 h at 20° C. Phosphorylation was measured using an antibody specific to p53 S15 phosphorylation (1:5000 Abcam ab38497), anti-rabbit HRP (1:1000 Cell Signalling Technology, 7074) and TMB (ab171522).

    Article Title: A bipartite bacterial virulence factor targets the complement system and neutrophil activation
    Article Snippet: Superdex 200 Increase 10/300 GL , Cytiva , 28990944. .. High binding microplate , Greiner Bio-One , 655061. ..

    Article Title: Early Binding of Anti-Amyloid Antibodies to CAA Drives Complement Activation, Inflammation and ARIA in Mice
    Article Snippet: .. Briefly, a 96-well high-binding microplate (Greiner Bio-One 96-Well High binding Polystyrene Microplates, #655085) was coated with rabbit anti-C1q (Abcam ab182451, 1:500, in 1x PBS buffer) overnight at 4 °C. ..

    Article Title: RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells.
    Article Snippet: .. Soluble antigen was diluted to 2 μg/mL in Carbonate Coating Buffer (0.2 M Carbonate-Bicarbonate, pH 9.4 (Thermo Fisher)) and 20 μL was dispensed into the wells of a 384-well High Binding microplate (Greiner). ..

    Blocking Assay:

    Article Title: Structure-Based Exploration of Selectivity for ATM Inhibitors in Huntington's Disease.
    Article Snippet: Our group has recently shown that brain-penetrant ataxia telangiectasia-mutated (ATM) kinase inhibitors may have potential as novel therapeutics for the treatment of Huntington’s disease (HD).. However, the previously described pyranonethioxanthenes (e.g., 4) failed to afford selectivity over a vacuolar protein sorting 34 (Vps34) kinase, an important kinase involved with autophagy.. Given that impaired autophagy has been proposed as a pathogenic mechanism of neurodegenerative diseases such as HD, achieving selectivity over Vps34 became an important objective for our program.

    Article Title: ATM kinase inhibitors and compositions and methods of use thereof
    Article Snippet: Reactions contained 0.75 nM ATM, 25 nM full length myc tagged p53 (Eurofins 23-034), 10 μM UltraPure ATP (Promega V915B) and serial dilutions of inhibitors (0.1% DMSO final) in 25 mM HEPES, pH 7.5. .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, high binding microplate (Greiner Bio One 781061), precoated with anti-myc capture antibody overnight (Millipore 05-724) diluted in 1:1000 in PBS, then blocked with Odyssey blocking buffer (LI-COR Biosciences 92740000) for 60 min at 20° C. The terminated reaction was allowed to capture for 2 h at 20° C. Phosphorylation was measured using an antibody specific to p53 S15 phosphorylation (1:5000 Abcam ab38497), anti-rabbit HRP (1:1000 Cell Signalling Technology, 7074) and TMB (ab171522). .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, high binding microplate (Greiner Bio One 781061), precoated with anti-myc capture antibody overnight (Millipore 05-724) diluted in 1:1000 in PBS, then blocked with Odyssey blocking buffer (LI-COR Biosciences 92740000) for 60 min at 20° C. The terminated reaction was allowed to capture for 2 h at 20° C. Phosphorylation was measured using an antibody specific to p53 S15 phosphorylation (1:5000 Abcam ab38497), anti-rabbit HRP (1:1000 Cell Signalling Technology, 7074) and TMB (ab171522).

    Incubation:

    Article Title: A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection.
    Article Snippet: ACE2 binding to RBD analyzed by titration ELISA ACE2 binding to the produced RBD variant antigens was analyzed by ELISA. .. Briefly, 0.1 ug of RBD per well was immobilized on a high binding Microplate (Greiner Bio-One GmbH, Austria.) and incubated ONat 4 °C. ..

    Phospho-proteomics:

    Article Title: ATM kinase inhibitors and compositions and methods of use thereof
    Article Snippet: Reactions contained 0.75 nM ATM, 25 nM full length myc tagged p53 (Eurofins 23-034), 10 μM UltraPure ATP (Promega V915B) and serial dilutions of inhibitors (0.1% DMSO final) in 25 mM HEPES, pH 7.5. .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, high binding microplate (Greiner Bio One 781061), precoated with anti-myc capture antibody overnight (Millipore 05-724) diluted in 1:1000 in PBS, then blocked with Odyssey blocking buffer (LI-COR Biosciences 92740000) for 60 min at 20° C. The terminated reaction was allowed to capture for 2 h at 20° C. Phosphorylation was measured using an antibody specific to p53 S15 phosphorylation (1:5000 Abcam ab38497), anti-rabbit HRP (1:1000 Cell Signalling Technology, 7074) and TMB (ab171522). .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, high binding microplate (Greiner Bio One 781061), precoated with anti-myc capture antibody overnight (Millipore 05-724) diluted in 1:1000 in PBS, then blocked with Odyssey blocking buffer (LI-COR Biosciences 92740000) for 60 min at 20° C. The terminated reaction was allowed to capture for 2 h at 20° C. Phosphorylation was measured using an antibody specific to p53 S15 phosphorylation (1:5000 Abcam ab38497), anti-rabbit HRP (1:1000 Cell Signalling Technology, 7074) and TMB (ab171522).

    Injection:

    Article Title: Antibody‐invertase Cross‐linkage Nanoparticles: A New Signal Tag for Point‐of‐Care Immunoassay of Alpha‐fetoprotein for Hepatocellular Carcinoma with Personal Glucometer
    Article Snippet: Methods based on immunoassays have been developed for cancer biomarker alpha-fetoprotein (AFP), but most involve complicated or stripping procedures and are unfavourable for routine use.. Herein we report the proof-of-concept of simple and point-of-care (POC) immunoassay for AFP in hepatocellular carcinoma on a portable personal glucometer (PGM) by using antibodyinvertase cross-linkage nanoparticles as the signal-generation tags.. Antibody-invertase cross-linkage nano tags were synthesized by using reverse micellar method with glutaraldehyde.



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    Greiner Bio high binding microtiter plate
    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
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    Image Search Results


    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot study

    doi: 10.3389/fimmu.2026.1745166

    Figure Lengend Snippet: Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Article Snippet: For the MPO-specific ELISA kit, a 96-well high-binding polystyrene microplate (F-bottom, Greiner Bio-One, #655061) was used.

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, Control