high binding clear bottom plates (Greiner Bio)
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High Binding Clear Bottom Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 838 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+binding+microplate/Microplate+384+Well+Ps+F-Bottom/pm41922952-83-2-6
Average 97 stars, based on 838 article reviews
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Binding Assay:Article Title: RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells Article Snippet: Following three 10-minute washes in TBS + 0.1% Tween-20, the blot was developed using 1-Step NPT/BCIP (Thermo Fisher) substrate and visualized on an AlphaImager HP (Protein Simple). .. Soluble antigen was diluted to 2 μg/mL in Carbonate Coating Buffer (0.2 M Carbonate-Bicarbonate, pH 9.4 (Thermo Fisher)) and 20 μL was dispensed into the wells of a 384-well Article Title: Structure-Based Exploration of Selectivity for ATM Inhibitors in Huntington's Disease. Article Snippet: Our group has recently shown that brain-penetrant ataxia telangiectasia-mutated (ATM) kinase inhibitors may have potential as novel therapeutics for the treatment of Huntington’s disease (HD).. However, the previously described pyranonethioxanthenes (e.g., 4) failed to afford selectivity over a vacuolar protein sorting 34 (Vps34) kinase, an important kinase involved with autophagy.. Given that impaired autophagy has been proposed as a pathogenic mechanism of neurodegenerative diseases such as HD, achieving selectivity over Vps34 became an important objective for our program. Article Title: A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection. Article Snippet: ACE2 binding to RBD analyzed by titration ELISA ACE2 binding to the produced RBD variant antigens was analyzed by ELISA. .. Briefly, 0.1 ug of RBD per well was immobilized on a Article Title: ATM kinase inhibitors and compositions and methods of use thereof Article Snippet: Reactions contained 0.75 nM ATM, 25 nM full length myc tagged p53 (Eurofins 23-034), 10 μM UltraPure ATP (Promega V915B) and serial dilutions of inhibitors (0.1% DMSO final) in 25 mM HEPES, pH 7.5. .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, Article Title: A bipartite bacterial virulence factor targets the complement system and neutrophil activation Article Snippet: Superdex 200 Increase 10/300 GL , Cytiva , 28990944. .. Article Title: Early Binding of Anti-Amyloid Antibodies to CAA Drives Complement Activation, Inflammation and ARIA in Mice Article Snippet: .. Briefly, a 96-well Article Title: RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells. Article Snippet: .. Soluble antigen was diluted to 2 μg/mL in Carbonate Coating Buffer (0.2 M Carbonate-Bicarbonate, pH 9.4 (Thermo Fisher)) and 20 μL was dispensed into the wells of a 384-well Blocking Assay:Article Title: Structure-Based Exploration of Selectivity for ATM Inhibitors in Huntington's Disease. Article Snippet: Our group has recently shown that brain-penetrant ataxia telangiectasia-mutated (ATM) kinase inhibitors may have potential as novel therapeutics for the treatment of Huntington’s disease (HD).. However, the previously described pyranonethioxanthenes (e.g., 4) failed to afford selectivity over a vacuolar protein sorting 34 (Vps34) kinase, an important kinase involved with autophagy.. Given that impaired autophagy has been proposed as a pathogenic mechanism of neurodegenerative diseases such as HD, achieving selectivity over Vps34 became an important objective for our program. Article Title: ATM kinase inhibitors and compositions and methods of use thereof Article Snippet: Reactions contained 0.75 nM ATM, 25 nM full length myc tagged p53 (Eurofins 23-034), 10 μM UltraPure ATP (Promega V915B) and serial dilutions of inhibitors (0.1% DMSO final) in 25 mM HEPES, pH 7.5. .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, Incubation:Article Title: A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection. Article Snippet: ACE2 binding to RBD analyzed by titration ELISA ACE2 binding to the produced RBD variant antigens was analyzed by ELISA. .. Briefly, 0.1 ug of RBD per well was immobilized on a Phospho-proteomics:Article Title: ATM kinase inhibitors and compositions and methods of use thereof Article Snippet: Reactions contained 0.75 nM ATM, 25 nM full length myc tagged p53 (Eurofins 23-034), 10 μM UltraPure ATP (Promega V915B) and serial dilutions of inhibitors (0.1% DMSO final) in 25 mM HEPES, pH 7.5. .. An aliquot of the terminated reaction (25 μl) was transferred to a 384-well, Injection:Article Title: Antibody‐invertase Cross‐linkage Nanoparticles: A New Signal Tag for Point‐of‐Care Immunoassay of Alpha‐fetoprotein for Hepatocellular Carcinoma with Personal Glucometer Article Snippet: Methods based on immunoassays have been developed for cancer biomarker alpha-fetoprotein (AFP), but most involve complicated or stripping procedures and are unfavourable for routine use.. Herein we report the proof-of-concept of simple and point-of-care (POC) immunoassay for AFP in hepatocellular carcinoma on a portable personal glucometer (PGM) by using antibodyinvertase cross-linkage nanoparticles as the signal-generation tags.. Antibody-invertase cross-linkage nano tags were synthesized by using reverse micellar method with glutaraldehyde. |
