Journal: iScience

Article Title: Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in Drosophila

doi: 10.1016/j.isci.2026.115209

Figure Lengend Snippet: Establishment of ePL-producing S2R+ cells (A) Synthesis pathway of ePLs. Two strategies to increase ePL production in Drosophila are shown. (i) Supplementation with 16-AG or 18-AG (1-alkylglycerol) or (ii) AGPS overexpression with 18-OH (fatty alcohol) supplementation. DHAP, dihydroxyacetone phosphate; MGAT, monoacylglycerol acyltransferase; CDP, cytidine diphosphate; CPT, choline phosphotransferase; and CEPT, choline/ethanolamine phosphotransferase. (B–E) Lipid composition in control and ePL-producing S2R+ cells ( n = 4) for total ePE area% of total PE (B), total ePC area% of total PC (C), and colormap for the composition of PE (D) and PC (E) species (area% of total PE or PC) in control cells (Control/S2R+), 100 μM 16-AG supplemented cells (16-AG/S2R+), dAGPS-expressing cells (Control/S2R+ dAGPS), and 100 μM 18-OH supplemented dAGPS -expressing cells (18-OH/S2R+ dAGPS). Phospholipid molecules are listed as PE (X:Y) or PC (X:Y), where X and Y denote the total number of carbon molecules in acyl chains and double bonds in acyl chains, respectively. Cross marks in (D, E) indicate phospholipid species under detectable levels. Each point represents a biological replicate. Data are presented as mean ± SEM. ∗∗∗ p < 0.01; Student’s t test. See also and and .

Article Snippet: 1- O -hexadecyl-rac-glycerol (16-AG) , Santa Cruz , sc-205917.

Techniques: Over Expression, Control, Expressing