Journal: The Cell Surface

Article Title: Pap1 is an adhesin involved in the interaction of Sporothrix schenckii and Sporothrix brasiliensis with the host

doi: 10.1016/j.tcsw.2025.100164

Figure Lengend Snippet: PAP1 expression under different growing conditions . Yeast-like cells were incubated under the following conditions at 37 °C: 1 h with either 1.0 μg mL −1 fibronection or thrombospondin 1; 1 h with a monolayer of HeLa cells; 1 h with 5 × 10 6 human PBMCs, or injected in the hemolymph of Galleria mellonella larvae and incubated for 24 h. Alternatively, biofilms were matured for 48 h at 37 °C. From these conditions, total RNA was extracted, cDNA synthesized with oligo(dT) primer (20 mer), and PAP1 expression quantified by RT-qPCR. Data were normalized using the expression of the gene encoding the ribosomal protein L6 and yeast-like cells growth in YPD at 37 °C as reference conditions (point zero on the Y axis). Results are means ± SD of three independent experiments performed in duplicate. The Dunnett's test and then the unpaired t -test were used for data analysis. * P < 0.05 when compared to the other growing conditions.

Article Snippet: In some experiments, HeLa cells (ATCC) were grown in monolayers in Eagle's Minimum Essential Medium (EMEM, Sigma-Aldrich) at 37 °C and 5 % CO 2 (v/v).

Techniques: Expressing, Incubation, Injection, Synthesized, Quantitative RT-PCR

Journal: The Cell Surface

Article Title: Pap1 is an adhesin involved in the interaction of Sporothrix schenckii and Sporothrix brasiliensis with the host

doi: 10.1016/j.tcsw.2025.100164

Figure Lengend Snippet: Adhesion to extracellular matrix components and HeLa cells of Sporothrix schenckii and Sporothrix brasiliensis PAP1 -silenced strains. The indicated extracellular matrix component was used to coat 96-well plates, then yeast-like cells were added, unbound cells were removed by extensive washing, and adherent cells were detected by ELISA with a primary anti-rHsp60 antibody. Control refers to wells coated with bovine serum albumin. Panel A contains data generated with S. schenckii, and WT is the 1099–18 ATCC MYA 4821 strain. Panel B shows results with S. brasiliensis cells, and the WT is the 5110 ATCC MYA 4823 strain. Alternatively, for both panels, 1 × 10 6 HeLa cells were placed per well, incubated 24 h at 37 °C and 5 % ( v /v) CO 2 , and used in the adhesion assays. Results are means ± SD of three biological replicates performed in duplicate. The Dunnett's test and then the unpaired t-test were used for data analysis. In both panels, * P < 0.05 when compared to WT or strains HSB1 and HSB2.

Article Snippet: In some experiments, HeLa cells (ATCC) were grown in monolayers in Eagle's Minimum Essential Medium (EMEM, Sigma-Aldrich) at 37 °C and 5 % CO 2 (v/v).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Generated, Incubation

Journal: Materials Today Bio

Article Title: Expanding the toolbox of bioorthogonal activation of photosensitizers for precise photodynamic therapy through transition metal-mediated deallylation

doi: 10.1016/j.mtbio.2026.102797

Figure Lengend Snippet: (a) Fluorescence confocal images of HeLa, 4T1, MCF-7, and NIH 3T3 cells after incubation with Pro-BDP-3 (5.0 μM) for 2 h with or without further incubation with RuL2 or RuL3 (2.5 μM) for a further 4 h (red fluorescence; λ ex = 633 nm, λ em = 650–900 nm). The cells being incubated with BDP-COOH (5.0 μM) for 2 h were used as the positive control. The cell nuclei were stained with Hoechst (1.0 μM) for 15 min (blue fluorescence; λ ex = 405 nm, λ em = 420–500 nm). Scale bar = 20 μm. (b) Corresponding mean red fluorescence intensities quantified by ImageJ. Data are reported as the mean ± standard error of the mean (SEM) for three independent experiments (∗∗∗∗p < 0.0001). (c) Fluorescence confocal images of HeLa, 4T1, MCF-7, and NIH 3T3 cells after the aforementioned treatments and further incubation with H 2 DCFDA (10 μM) for 30 min, followed by light irradiation (λ > 610 nm, 25.8 mW/cm 2 ) for 8 min to give a total fluence of 12 J/cm 2 (green fluorescence; λ ex = 488 nm, λ em = 493–550 nm). Scale bar = 20 μm. (d) Corresponding mean green fluorescence intensities of DCF quantified by ImageJ. Data are reported as the mean ± SEM for three independent experiments (∗∗∗∗p < 0.0001). (e) Dark and photo (λ > 610 nm, 25.8 mW/cm 2 , 12 J/cm 2 ) cytotoxicity of BDP-COOH , Pro-BDP-3 , RuL2 , Pro-BDP-3 + RuL2 , RuL3 , and Pro-BDP-3 + RuL3 against HeLa, 4T1, MCF-7, and NIH 3T3 cells. The cells were incubated with BDP-COOH , Pro-BDP-3 , RuL2 , or RuL3 for 2 h. For Pro-BDP-3 + RuL2 and Pro-BDP-3 + RuL3 , the cells were first incubated with Pro-BDP-3 for 2 h and then with RuL2 or RuL3 (0.5 equiv.) for a further 4 h. Data are expressed as the mean ± SEM of three independent experiments, each performed in quadruplicate. (f) Photocytotoxicity of these agents at 5.0 μM and the combination treatments at 5.0 μM of Pro-BDP-3 against the four cell lines. The rightmost figure compiles the results for Pro-BDP-3 + RuL3 (∗∗∗∗p < 0.0001). Data are expressed as the mean ± SEM of three independent experiments, each performed in quadruplicate. (g) Live/dead cell viability assay using calcein-AM and PI. The cells were treated as described above, followed by incubation with calcein-AM (1 μM) and PI (2 μM) in binding buffer (2 mL) at 37 °C for 30 min. The live cells were indicated by the green fluorescence of calcein-AM (λ ex = 488 nm, λ em = 493–550 nm), while the dead cells were indicated by the red fluorescence of PI (λ ex = 561 nm, λ em = 600–800 nm). Scale bar = 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The HeLa human cervical cancer cells (ATCC, CCL-2), 4T1 murine mammary carcinoma cells (ATCC, CRL-2539), MCF-7 human breast cancer cells (ATCC, HTB-22), and NIH 3T3 murine embryonic fibroblast cells were maintained in Dulbecco's modified Eagle's medium (DMEM, ThermoFisher, cat. no. 11965092) supplemented with fetal calf serum (10 %) and penicillin-streptomycin (100 unit/mL and 100 μg/mL, respectively).

Techniques: Fluorescence, Incubation, Positive Control, Staining, Irradiation, Viability Assay, Binding Assay