Journal: bioRxiv

Article Title: Single injection of modified self-amplifying RNA encoding a CD19 bispecific T cell engager mediates long-term malignant B cell clearance

doi: 10.64898/2026.04.18.719371

Figure Lengend Snippet: (A) Schematic of a single intravenous administration of modified saRNA-BiTE encapsulated in LNPs inducing sustained BiTE production and durable B cell clearance in patients. NSP, VEEV non-structural protein; SP, murine Igκ signal peptide. (B) saRNA-BiTE and mRNA-BiTE-induced killing of Nalm6 (target), Raji (target), and K562 (bystander) cells in co-cultures with primary human T cells when treated with transfected HeLa cell media at a 33 ng RNA dose collected on day 1 or day 7 post transfection. (C) saRNA-BiTE and mRNA-BiTE-induced killing of Nalm6 cells in Nalm6 and primary human T cell co-cultures when treated with HeLa cell media at a 33 ng RNA dose collected from different time points over a week post transfection. (D) IFNγ levels in Nalm6 and primary human T cell co-cultures treated with HeLa cell media at a 33 ng RNA dose collected on day 1 and day 7 post transfection. Data are presented as mean ± s.d. (n = 2 to 3 biological replicates). Statistical significance was determined using two-way ANOVA with Tukey’s multiple comparison. Significance is only labeled for comparison between saRNA-BiTE and mRNA-BiTE for panels C and D.

Article Snippet: HeLa cells (ATCC) were cultured in DMEM (Corning) supplemented with 10% FBS and 1% Pen-Strep.

Techniques: Modification, Transfection, Comparison, Labeling

Journal: bioRxiv

Article Title: Single injection of modified self-amplifying RNA encoding a CD19 bispecific T cell engager mediates long-term malignant B cell clearance

doi: 10.64898/2026.04.18.719371

Figure Lengend Snippet: (A) Denaturing gel electrophoresis showing full-length saRNA-BiTE (∼ 9400 nt) and mRNA-BiTE (∼ 1900 nt) before and after cellulose purification with good integrity. (B) Dot blot of saRNA-BiTE and mRNA-BiTE demonstrating under limit-of-detection levels of dsRNA after cellulose purification. (C) Physical properties of saRNA-BiTE, mRNA-BiTE, and saRNA-mCherry LNPs measured by DLS and encapsulation efficiency. DLS data are presented as mean ± s.d. (n = 5 technical replicates). (D) Western blotting confirming secretion of full-length BiTE from saRNA-BiTE and mRNA-BiTE transfected HeLa cells but not saRNA-mCherry transfected cells.

Article Snippet: HeLa cells (ATCC) were cultured in DMEM (Corning) supplemented with 10% FBS and 1% Pen-Strep.

Techniques: Nucleic Acid Electrophoresis, Purification, Dot Blot, Encapsulation, Western Blot, Transfection

Journal: bioRxiv

Article Title: Single injection of modified self-amplifying RNA encoding a CD19 bispecific T cell engager mediates long-term malignant B cell clearance

doi: 10.64898/2026.04.18.719371

Figure Lengend Snippet: (A) BiTE-induced killing of Nalm6 cells (target) in Nalm6 and primary human T cell co-cultures when treated with HeLa cell media collected from different time points over a week post transfection at all RNA doses. (B) BiTE-induced killing of Raji cells (target) in Raji and primary human T cell co-cultures when treated with HeLa cell media collected on day 1 or day 7 post transfection. (C) BiTE-induced killing of K562 cells (bystander) in K562 and primary human T cell co-cultures when treated with HeLa cell media collected on day 1 post transfection. (D) IFNγ levels in Nalm6 and primary human T cell co-cultures treated with HeLa cell media collected on day 1 and day 7 post transfection at all RNA doses. Data are presented as mean ± s.d. (n = 2 to 3 biological replicates). Statistical significance was determined using two-way ANOVA with Tukey’s multiple comparison. Significance is only labeled for comparison between saRNA-BiTE and mRNA-BiTE at each RNA dose level.

Article Snippet: HeLa cells (ATCC) were cultured in DMEM (Corning) supplemented with 10% FBS and 1% Pen-Strep.

Techniques: Transfection, Comparison, Labeling

Journal: Nucleic Acids Research

Article Title: Comparative biochemical analysis of UHRF proteins reveals molecular mechanisms that uncouple UHRF2 from DNA methylation maintenance

doi: 10.1093/nar/gky151

Figure Lengend Snippet: UHRF1 and UHRF2 diverge in their ability to ubiquitinate nucleosomal histones. ( A ) Chromatin association assays for FLAG-tagged UHRF2 (WT) or the indicated mutants from asynchronously growing HeLa cells. Mock treatment represents an empty vector control. ( B ) Comparative in vitro ubiquitination of H3 (1–32) K9me2 (left) and HeLa mononucleosomes (right) by UHRF1 and UHRF2. Moles of H3 are equivalent in these reactions.

Article Snippet: For in vitro ubiquitination of HeLa mononucleosomes, reactions were run in ubiquitin assay buffer containing 1.5 μM E3, 50 nM E1 UBA1, 667 nM E2 UBCH5A, 5 μM TAMRA-ubiquitin, and 0.5 μM HeLa mononucleosomes (Epicypher).

Techniques: Plasmid Preparation, Control, In Vitro, Ubiquitin Proteomics

Journal: EBioMedicine

Article Title: A truncating MEIOB mutation responsible for familial primary ovarian insufficiency abolishes its interaction with its partner SPATA22 and their recruitment to DNA double-strand breaks

doi: 10.1016/j.ebiom.2019.03.075

Figure Lengend Snippet: The MEIOB-truncating mutation disrupts MEIOB-SPATA22 recruitment to DNA damage foci. a-b- The truncated form of MEIOB is stable when expressed in HeLa cells. a- Western blot showing the expression of the wild-type (MEIOB MF ) and the truncated forms (MEIOB MF-mut ) of MEIOB transfected in HeLa cells. The ProSieve QuadColor protein marker 4,6 kDa–300 kDa (Lonza) was run on the left (Ladder). b- MEIOB MF or MEIOB MF-mut and SPATA22 were co-expressed from a single plasmid MEIOB∧SPATA22 in HeLa cells before exposure to etoposide (25 μM). Cells were fixed in PFA without pre-permeabilisation to retain both cytoplasmic and nuclear fractions. Antibodies were used to immunostain MEIOB (red) and SPATA22 (white). Yellow arrows highlight transfected cells expressing both MEIOB and SPATA22. c-d- The truncation prevents MEIOB recruitment with SPATA22 to DSBs. MEIOB MF or MEIOB MF-mut and SPATA22 HA were expressed in HeLa cells before exposure to etoposide. Cells were permeabilised before fixation in PFA to analyse the chromatin-associated fraction of proteins. RPA foci are formed at DNA damage sites. c- MEIOB or SPATA22 alone are not recruited to DNA damage sites. d- When co-expressed, MEIOB MF and SPATA22 are recruited to DNA-damage sites and colocalise with RPA. This colocalisation is not observed for the truncated MEIOB MF-mut . Antibodies are used to immunostain MEIOB (red), SPATA22 (white), and RPA (green). Scale bar = 10 μm in all panels. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: HeLa cells were used for minigene assays (CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030).

Techniques: Mutagenesis, Western Blot, Expressing, Transfection, Marker, Plasmid Preparation