hela Search Results


hela cells  (ATCC)
99
ATCC hela cells
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela s3 cells  (CLS Cell Lines Service GmbH)
94
CLS Cell Lines Service GmbH hela s3 cells
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Hela S3 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela atcc  (ATCC)
99
ATCC hela atcc
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Hela Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela  (ATCC)
99
ATCC hela
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Hela, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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rabbit anti zif268  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology rabbit anti zif268
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Rabbit Anti Zif268, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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helas3 cells  (ATCC)
97
ATCC helas3 cells
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Helas3 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sc 222 wb  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sc 222 wb
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Sc 222 Wb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
sc 222 wb - by Bioz Stars, 2026-06
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hela 229 cells 602  (ATCC)
96
ATCC hela 229 cells 602
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Hela 229 Cells 602, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela 229 cells 602 - by Bioz Stars, 2026-06
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human cervical hela cancer cells  (DSMZ)
96
DSMZ human cervical hela cancer cells
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Human Cervical Hela Cancer Cells, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cervical hela cancer cells - by Bioz Stars, 2026-06
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hela cells lysate  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology hela cells lysate
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Hela Cells Lysate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela cells  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology hela cells
(A) Immunostaining of iPLA 2 in <t>HeLa</t> cells infected with <t>the</t> <t>ATCC</t> 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.
Hela Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Immunostaining of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.

Journal: bioRxiv

Article Title: OmpA controls intracellular survival of Acinetobacter baumannii through TFEB activation and lysosomal remodeling

doi: 10.64898/2026.04.18.719357

Figure Lengend Snippet: (A) Immunostaining of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (B) Immunoblot analysis of inactive and active forms of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h. (C) Enzymatic activity of iPLA 2 in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P =0.025 for ATCC 17978 vs. CTL, ** P =0.05 for ATCC 17978 vs. ATCC 1797 8 + BEL (5 µM), *** P =0.042 for ATCC 17978 vs. ATCC 17978 + BEL (10 µM). (D) Cox-2 enzymatic activity in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. (* P =0.0011 for ATCC 17978 vs. CTL, * P =0.049 for CTL vs. ATCC 17978 + BEL (5 µM), * P =0.0096 for CTL vs. ATCC 17978 + NS, ** P =0.0005 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.001 for ATCC 17978 vs. ATCC 17978 + NS. ( E ) PGE2 production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL and NS. * P =0.004 for ATCC 17978 vs. CTL, ** P =0.0417 for ATCC 17978 vs. ATCC 17978 + BEL (5 µM), *** P =0.0015 for ATCC 17978 vs. ATCC 17978 + NS. CTL: control; Str: staurosporine; BEL: bromoelone lactone; NS: cyclooxygenase inhibitor; Cox-2: cyclooxygenase-2; PGE2: prostaglandin 2.

Article Snippet: Briefly, HeLa cells plated on coverslips were incubated with A. baumannii ATCC 17978 (1 x 10 7 CFU/mL) or stauroporine (10 mg/L, apopotosis inducer) for 24 h at 37 °C, 5% CO 2 .

Techniques: Immunostaining, Infection, Western Blot, Activity Assay, Control

(A) Immnunoblot analysis of TFEB in HeLa cells infected with the ATCC 17978 strain for 2 h in the presence or absence of BEL (5 µM). * P =0.041 for ATCC 17978 vs. ATCC 17978 + BEL. (B) TFEB nuclear translocation in TFEB GFP+ cells infected with the ATCC 17978 strain for 2 h in the presence or absence of BEL (5 µM). * P =0.0162 for CTL vs. ATCC 17978, * P =0.0452 for CTL vs. ATCC 17978, ** P =0.0497 for ATCC 17978 vs. ATCC 17978 + BEL. (C) Immunoblot analysis of TFEB in HeLa cells transfected with scrambled or iPLA 2 siRNA for 48 h and infected with the ATCC 17978 strain for 2 h. * P =0.0012 for CTL vs. siRNA of iPLA 2 . BEL: bromoelone lactone; Sc: scramble.

Journal: bioRxiv

Article Title: OmpA controls intracellular survival of Acinetobacter baumannii through TFEB activation and lysosomal remodeling

doi: 10.64898/2026.04.18.719357

Figure Lengend Snippet: (A) Immnunoblot analysis of TFEB in HeLa cells infected with the ATCC 17978 strain for 2 h in the presence or absence of BEL (5 µM). * P =0.041 for ATCC 17978 vs. ATCC 17978 + BEL. (B) TFEB nuclear translocation in TFEB GFP+ cells infected with the ATCC 17978 strain for 2 h in the presence or absence of BEL (5 µM). * P =0.0162 for CTL vs. ATCC 17978, * P =0.0452 for CTL vs. ATCC 17978, ** P =0.0497 for ATCC 17978 vs. ATCC 17978 + BEL. (C) Immunoblot analysis of TFEB in HeLa cells transfected with scrambled or iPLA 2 siRNA for 48 h and infected with the ATCC 17978 strain for 2 h. * P =0.0012 for CTL vs. siRNA of iPLA 2 . BEL: bromoelone lactone; Sc: scramble.

Article Snippet: Briefly, HeLa cells plated on coverslips were incubated with A. baumannii ATCC 17978 (1 x 10 7 CFU/mL) or stauroporine (10 mg/L, apopotosis inducer) for 24 h at 37 °C, 5% CO 2 .

Techniques: Infection, Translocation Assay, Western Blot, Transfection

(A) LPC production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P <0.0001 for ATCC 17978 vs. CTL, * P =0.032 for CTL vs. ATCC 17978 + BEL, ** P =0.048 for ATCC 17978 vs. ATCC 17978 + BEL. (B) TFEB activation in TFEB GFP+ cells treated with LPC (0.5 µM) for 1 h. * P <0.0001 for 0 h vs. 1 h. CTL: control; BEL: bromoelone lactone; LPC: lysophosphatidylcholine.

Journal: bioRxiv

Article Title: OmpA controls intracellular survival of Acinetobacter baumannii through TFEB activation and lysosomal remodeling

doi: 10.64898/2026.04.18.719357

Figure Lengend Snippet: (A) LPC production in HeLa cells infected with the ATCC 17978 strain for 24 h, in the presence or absence of BEL. * P <0.0001 for ATCC 17978 vs. CTL, * P =0.032 for CTL vs. ATCC 17978 + BEL, ** P =0.048 for ATCC 17978 vs. ATCC 17978 + BEL. (B) TFEB activation in TFEB GFP+ cells treated with LPC (0.5 µM) for 1 h. * P <0.0001 for 0 h vs. 1 h. CTL: control; BEL: bromoelone lactone; LPC: lysophosphatidylcholine.

Article Snippet: Briefly, HeLa cells plated on coverslips were incubated with A. baumannii ATCC 17978 (1 x 10 7 CFU/mL) or stauroporine (10 mg/L, apopotosis inducer) for 24 h at 37 °C, 5% CO 2 .

Techniques: Infection, Activation Assay, Control

(A) Inhibition of calcium entry through ORAI channel reduces TFEB activation in TFEB GFP+ cells infected with the ATCC 17978 strain for 2 h. * P =0.05 for ATCC 17978 vs. ATCC 17978 + GSK. 7975A (B) Chelation of intracellular calcium with EGTA reduces TFEB activation in TFEB GFP+ cells infected with the ATCC 17978 strain for 2 h. * P <0.0001 for CTL vs. ATCC 17978, * P <0.0001 for CTL vs ATCC 17978 + EGTA, ** P <0.0001 for ATCC 17978 vs. ATCC 17978 + EGTA. (C) Immunoblot analysis of ORAI and TFEB in HeLa cells transfected with scrambled or iPLA 2 siRNA for 48 h and infected with the ATCC 17978 strain for 2 h. * P =0.0093 for ATCC 17978 vs. siRNA ORAI. (D) Lysosomal analysis in HeLa cells infected with the ATCC 17978 strain for 2 h. Acidic organelles were labeled with LysoTracker Red and mitochondria were labeled with MitoTracker Green. CTL: control, EGTA: Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt.

Journal: bioRxiv

Article Title: OmpA controls intracellular survival of Acinetobacter baumannii through TFEB activation and lysosomal remodeling

doi: 10.64898/2026.04.18.719357

Figure Lengend Snippet: (A) Inhibition of calcium entry through ORAI channel reduces TFEB activation in TFEB GFP+ cells infected with the ATCC 17978 strain for 2 h. * P =0.05 for ATCC 17978 vs. ATCC 17978 + GSK. 7975A (B) Chelation of intracellular calcium with EGTA reduces TFEB activation in TFEB GFP+ cells infected with the ATCC 17978 strain for 2 h. * P <0.0001 for CTL vs. ATCC 17978, * P <0.0001 for CTL vs ATCC 17978 + EGTA, ** P <0.0001 for ATCC 17978 vs. ATCC 17978 + EGTA. (C) Immunoblot analysis of ORAI and TFEB in HeLa cells transfected with scrambled or iPLA 2 siRNA for 48 h and infected with the ATCC 17978 strain for 2 h. * P =0.0093 for ATCC 17978 vs. siRNA ORAI. (D) Lysosomal analysis in HeLa cells infected with the ATCC 17978 strain for 2 h. Acidic organelles were labeled with LysoTracker Red and mitochondria were labeled with MitoTracker Green. CTL: control, EGTA: Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt.

Article Snippet: Briefly, HeLa cells plated on coverslips were incubated with A. baumannii ATCC 17978 (1 x 10 7 CFU/mL) or stauroporine (10 mg/L, apopotosis inducer) for 24 h at 37 °C, 5% CO 2 .

Techniques: Inhibition, Activation Assay, Infection, Western Blot, Transfection, Labeling, Control

(A) Ammonia production in HeLa cells infected with the ATCC 17978 strain for 2 h. * P =0.011 for ATCC 17978 vs. CTL. (B) Intracellular replication of the ATCC 17978 strain in HeLa cells infected at 2, 4 and 8 h post-infection, in the presence or absence of bafilomycin. Data are presented as fold change relative to the intracellular CFU at 2 h. * P =0.033 for ATCC 17978 vs. ATCC 17978 + bafilomycin. (C) Lysosomal pH measurement of HeLa cells infected for 2 h with the ATCC 17978 strain stained with LysoSensor Yellow/Blue. * P =0.0164 for ATCC 17978 vs. CTL. (D) pH measurement of LB medium over 24 h in the presence of the ATCC 17978 strain. (E) A. baumannii ATCC 17978 in LB medium during 24 h under acidic or neutral conditions. CTL: control.

Journal: bioRxiv

Article Title: OmpA controls intracellular survival of Acinetobacter baumannii through TFEB activation and lysosomal remodeling

doi: 10.64898/2026.04.18.719357

Figure Lengend Snippet: (A) Ammonia production in HeLa cells infected with the ATCC 17978 strain for 2 h. * P =0.011 for ATCC 17978 vs. CTL. (B) Intracellular replication of the ATCC 17978 strain in HeLa cells infected at 2, 4 and 8 h post-infection, in the presence or absence of bafilomycin. Data are presented as fold change relative to the intracellular CFU at 2 h. * P =0.033 for ATCC 17978 vs. ATCC 17978 + bafilomycin. (C) Lysosomal pH measurement of HeLa cells infected for 2 h with the ATCC 17978 strain stained with LysoSensor Yellow/Blue. * P =0.0164 for ATCC 17978 vs. CTL. (D) pH measurement of LB medium over 24 h in the presence of the ATCC 17978 strain. (E) A. baumannii ATCC 17978 in LB medium during 24 h under acidic or neutral conditions. CTL: control.

Article Snippet: Briefly, HeLa cells plated on coverslips were incubated with A. baumannii ATCC 17978 (1 x 10 7 CFU/mL) or stauroporine (10 mg/L, apopotosis inducer) for 24 h at 37 °C, 5% CO 2 .

Techniques: Infection, Staining, Control

(A) Adherence and invasion of ATCC 17978 and ATCC 17978 ΔOmpA strains in HeLa cells for 2 h. * P =0.003 for ATCC 17978 vs. ATCC 17978 ΔOmpA (adherence), * P =0.032 for ATCC 17978 vs. ATCC 17978 ΔOmpA (invasion). (B) Enzymatic activity of iPLA 2 in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 24 h. * P =0.025 for CTL vs. ATCC 17978, * P =0.0255 for CTL vs. ATCC 17978 ΔOmpA. (C) LPC production in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 24 h. * P <0.001 for CTL vs. ATCC 17978, * P =0.043 for CTL vs. ATCC 17978 ΔOmpA, ** P =0.03 for ATCC 17978 vs. ATCC 17978 ΔOmpA. (D) Immunoblot analysis of TFEB in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 2 h. * P =0.0424 for CTL vs. ATCC 17978. CTL: control.

Journal: bioRxiv

Article Title: OmpA controls intracellular survival of Acinetobacter baumannii through TFEB activation and lysosomal remodeling

doi: 10.64898/2026.04.18.719357

Figure Lengend Snippet: (A) Adherence and invasion of ATCC 17978 and ATCC 17978 ΔOmpA strains in HeLa cells for 2 h. * P =0.003 for ATCC 17978 vs. ATCC 17978 ΔOmpA (adherence), * P =0.032 for ATCC 17978 vs. ATCC 17978 ΔOmpA (invasion). (B) Enzymatic activity of iPLA 2 in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 24 h. * P =0.025 for CTL vs. ATCC 17978, * P =0.0255 for CTL vs. ATCC 17978 ΔOmpA. (C) LPC production in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 24 h. * P <0.001 for CTL vs. ATCC 17978, * P =0.043 for CTL vs. ATCC 17978 ΔOmpA, ** P =0.03 for ATCC 17978 vs. ATCC 17978 ΔOmpA. (D) Immunoblot analysis of TFEB in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 2 h. * P =0.0424 for CTL vs. ATCC 17978. CTL: control.

Article Snippet: Briefly, HeLa cells plated on coverslips were incubated with A. baumannii ATCC 17978 (1 x 10 7 CFU/mL) or stauroporine (10 mg/L, apopotosis inducer) for 24 h at 37 °C, 5% CO 2 .

Techniques: Activity Assay, Infection, Western Blot, Control

(A and B) Lysosomal analysis in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 2 h. Acidic organelles were labeled with LysoTracker Red and mitochondria were labeled with MitoTracker Green. Lysosomal staining in infected HeLa cells is presented in the bar graph as a percentage relative to non-infected control cells. * P =0.0111 for ATCC 17978 vs CTL. ** P =0.0140 for ATCC 17978 vs. ATCC 17978 ΔOmpA. (C) Ammonia production in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 2 h.* P =0.011 for ATCC 17978 vs CTL, ** P =0.03031 for ATCC 17978 vs ATCC 17978 ΔOmpA. (D) Intracellular replication of ATCC 17978 or ATCC 17978 ΔOmpA strains in HeLa cells infected at 2, 4 and 8 h post-infection, in the presence or absence of bafilomycin. Data are expressed as fold change in intracellular CFU relative to 2 h.* P =0.033 for ATCC 17978 vs. ATCC 17978 + bafilomycin. (E) Lysosomal pH measurement of HeLa cells infected with the ATCC 17978 or ATCC 17978 ΔOmpA strains stained with LysoSensor Yellow/Blue dextran for 2 h. * P =0.0164 for ATCC 17978 vs. CTL. CTL: control.

Journal: bioRxiv

Article Title: OmpA controls intracellular survival of Acinetobacter baumannii through TFEB activation and lysosomal remodeling

doi: 10.64898/2026.04.18.719357

Figure Lengend Snippet: (A and B) Lysosomal analysis in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 2 h. Acidic organelles were labeled with LysoTracker Red and mitochondria were labeled with MitoTracker Green. Lysosomal staining in infected HeLa cells is presented in the bar graph as a percentage relative to non-infected control cells. * P =0.0111 for ATCC 17978 vs CTL. ** P =0.0140 for ATCC 17978 vs. ATCC 17978 ΔOmpA. (C) Ammonia production in HeLa cells infected with ATCC 17978 or ATCC 17978 ΔOmpA strains for 2 h.* P =0.011 for ATCC 17978 vs CTL, ** P =0.03031 for ATCC 17978 vs ATCC 17978 ΔOmpA. (D) Intracellular replication of ATCC 17978 or ATCC 17978 ΔOmpA strains in HeLa cells infected at 2, 4 and 8 h post-infection, in the presence or absence of bafilomycin. Data are expressed as fold change in intracellular CFU relative to 2 h.* P =0.033 for ATCC 17978 vs. ATCC 17978 + bafilomycin. (E) Lysosomal pH measurement of HeLa cells infected with the ATCC 17978 or ATCC 17978 ΔOmpA strains stained with LysoSensor Yellow/Blue dextran for 2 h. * P =0.0164 for ATCC 17978 vs. CTL. CTL: control.

Article Snippet: Briefly, HeLa cells plated on coverslips were incubated with A. baumannii ATCC 17978 (1 x 10 7 CFU/mL) or stauroporine (10 mg/L, apopotosis inducer) for 24 h at 37 °C, 5% CO 2 .

Techniques: Infection, Labeling, Staining, Control