Review



halotag7 insert  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 91
      Buy from Supplier

    Structured Review

    Addgene inc halotag7 insert
    a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
    Halotag7 Insert, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/halotag7+insert/pET28a-HaloTag7-SnoopLigase+(Plasmid+%23105627)/bio_rxiv__2022__10__24__513449-197-5-15
    Average 91 stars, based on 4 article reviews
    halotag7 insert - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging"

    Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging

    Journal: bioRxiv

    doi: 10.1101/2022.10.24.513449

    a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
    Figure Legend Snippet: a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

    Techniques Used: Construct, Expressing, Control, Incubation, Imaging, Fluorescence, Plasmid Preparation

    Related Articles

    Plasmid Preparation:

    Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging
    Article Snippet: The pcDNA3-HaloTag7-Sam68 plasmid was prepared using the NEBuilder HiFi DNA Assembly kit (NEB). .. The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group, Heidelberg) as templates. .. The pAV-U6-centromere-sgRNA plasmids (sgRNA, sgRNA-RhoBAST-1, sgRNA-RhoBAST-2, sgRNA-RhoBAST 2 ) were prepared by inserting a double-digested gene fragment containing the U6 promotor sequence, the sgRNA construct and a polyT stretch for termination into the BamHI and XhoI restriction sites of the pAV-U6+27 plasmid (Addgene, plasmid #25709).

    Amplification:

    Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging
    Article Snippet: The pcDNA3-HaloTag7-Sam68 plasmid was prepared using the NEBuilder HiFi DNA Assembly kit (NEB). .. The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group, Heidelberg) as templates. .. The pAV-U6-centromere-sgRNA plasmids (sgRNA, sgRNA-RhoBAST-1, sgRNA-RhoBAST-2, sgRNA-RhoBAST 2 ) were prepared by inserting a double-digested gene fragment containing the U6 promotor sequence, the sgRNA construct and a polyT stretch for termination into the BamHI and XhoI restriction sites of the pAV-U6+27 plasmid (Addgene, plasmid #25709).

    Polymerase Chain Reaction:

    Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging
    Article Snippet: The pcDNA3-HaloTag7-Sam68 plasmid was prepared using the NEBuilder HiFi DNA Assembly kit (NEB). .. The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group, Heidelberg) as templates. .. The pAV-U6-centromere-sgRNA plasmids (sgRNA, sgRNA-RhoBAST-1, sgRNA-RhoBAST-2, sgRNA-RhoBAST 2 ) were prepared by inserting a double-digested gene fragment containing the U6 promotor sequence, the sgRNA construct and a polyT stretch for termination into the BamHI and XhoI restriction sites of the pAV-U6+27 plasmid (Addgene, plasmid #25709).



    Similar Products

    halotag7 insert  (Addgene inc)
    91
    Addgene inc halotag7 insert
    a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
    Halotag7 Insert, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/halotag7+insert/pET28a-HaloTag7-SnoopLigase+(Plasmid+%23105627)/bio_rxiv__2022__10__24__513449-197-5-15
    Average 91 stars, based on 1 article reviews
    halotag7 insert - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

    Journal: bioRxiv

    Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging

    doi: 10.1101/2022.10.24.513449

    Figure Lengend Snippet: a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

    Article Snippet: The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group, Heidelberg) as templates.

    Techniques: Construct, Expressing, Control, Incubation, Imaging, Fluorescence, Plasmid Preparation