infectedwith h2b mruby lentivirus  (Addgene inc)

Journal: Cell reports

Article Title: Regulation of transcription patterns, poly(ADP-ribose), and RNA-DNA hybrids by the ATM protein kinase

doi: 10.1016/j.celrep.2024.113896

Figure Lengend Snippet:

Article Snippet: For R-ChIP studies, human RNaseH1 was expressed using a lentivirus derived from pTP5135, containing a tet-on allele of RNaseH D210N in a pLenti Hygro H2B mRuby backbone. pLentiPGK Hygro DEST H2B-mRuby2 was a gift from Markus Covert (Addgene plasmid # 90236; http://n2t.net/addgene:90236 ; RRID:Addgene_90236).

Techniques: Recombinant, Protease Inhibitor, Binding Assay, Magnetic Beads, Single Cell Gel Electrophoresis, Multiplex Assay, Software, RNA Extraction