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h 3501  (Vector Laboratories)


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    Vector Laboratories h 3501
    H 3501, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h+3501/Alcian+Blue+(pH+2%2E5)+Stain+Kit/pm41984591-625-19-17
    Average 95 stars, based on 95 article reviews
    h 3501 - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: Regulatory elements of pancreas development license the initiation of pancreatic ductal adenocarcinoma
    Article Snippet: H&E and alcian blue stainings were performed according to the manufacturer’s instructions (Abcam, ab245880 for H&E and Vector Laboratories, H-3501 for alcian blue) after which slides were mounted with Entellan (EMD Millipore, 1.07960.0500).

    Article Title: Regulatory elements in the Sox9 locus license the initiation of pancreatic ductal adenocarcinoma.
    Article Snippet: H&E and Alcian blue stainings were performed according to the manufacturer’s instructions (Abcam, ab245880 for H&E and Vector Laboratories, H-3501 for Alcian blue) after which slides were mounted with Entellan (EMD Millipore, 1.07960.0500).

    Staining:

    Article Title: Fexuprazan mitigates NSAID-induced small intestinal injury by restoring intestinal barrier integrity in mice.
    Article Snippet: .. Alcian blue staining was performed in accordance with the manufacturer’s instructions provided by the vector lab (H-3501, Vector Laboratories). ..



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    ( a ) Representative images of <t>IHC</t> <t>staining</t> for Ki-67 and <t>Alcian</t> blue (AB) in colon tissues from mice under SPF (n = 4) or GF (n = 3) housing conditions. Scale bars, 50 μm. ( b ) Quantification of Ki-67 + cells and AB + goblet cells across intestinal segments (duodenum, jejunum, ileum, and colon) in SPF and GF mice. All data are presented as mean ± s.e.m. of at least 3 biological replicates. P values were calculated using unpaired (two-tailed) t -test.
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    alcian blue (ph 2.5) stain kit  (Vector Laboratories)
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    ( a ) Representative images of <t>IHC</t> <t>staining</t> for Ki-67 and <t>Alcian</t> blue (AB) in colon tissues from mice under SPF (n = 4) or GF (n = 3) housing conditions. Scale bars, 50 μm. ( b ) Quantification of Ki-67 + cells and AB + goblet cells across intestinal segments (duodenum, jejunum, ileum, and colon) in SPF and GF mice. All data are presented as mean ± s.e.m. of at least 3 biological replicates. P values were calculated using unpaired (two-tailed) t -test.
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    Image Search Results


    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Journal: Gut Microbes

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis

    doi: 10.1080/19490976.2026.2662638

    Figure Lengend Snippet: Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Article Snippet: Alcian blue staining was performed to visualize acidic mucopolysaccharides and glycosaminoglycans in goblet cells of colon sections using the Alcian Blue Stain Kit (pH 2.5, Cat# H-3501, Vector Laboratories), following the manufacturer’s instructions.

    Techniques: Staining, Western Blot, Quantitative RT-PCR, Expressing

    ( a ) Representative images of IHC staining for Ki-67 and Alcian blue (AB) in colon tissues from mice under SPF (n = 4) or GF (n = 3) housing conditions. Scale bars, 50 μm. ( b ) Quantification of Ki-67 + cells and AB + goblet cells across intestinal segments (duodenum, jejunum, ileum, and colon) in SPF and GF mice. All data are presented as mean ± s.e.m. of at least 3 biological replicates. P values were calculated using unpaired (two-tailed) t -test.

    Journal: Nature Microbiology

    Article Title: Weaning drives microbiome-mediated epigenetic regulation to shape immune memory in mice

    doi: 10.1038/s41564-026-02295-6

    Figure Lengend Snippet: ( a ) Representative images of IHC staining for Ki-67 and Alcian blue (AB) in colon tissues from mice under SPF (n = 4) or GF (n = 3) housing conditions. Scale bars, 50 μm. ( b ) Quantification of Ki-67 + cells and AB + goblet cells across intestinal segments (duodenum, jejunum, ileum, and colon) in SPF and GF mice. All data are presented as mean ± s.e.m. of at least 3 biological replicates. P values were calculated using unpaired (two-tailed) t -test.

    Article Snippet: Alcian blue (AB) (pH 2.5) staining (Vector Labs, H-3501) was conducted following manufacturer instructions.

    Techniques: Immunohistochemistry, Two Tailed Test