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vectashield antifade mounting medium with dapi  (Vector Laboratories)


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    Vector Laboratories vectashield antifade mounting medium with dapi
    Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h+1200/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/custom%40h-1200%4042668564
    Average 98 stars, based on 21944 article reviews
    vectashield antifade mounting medium with dapi - by Bioz Stars, 2026-09
    98/100 stars

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    Incubation:

    Article Title: GDF15 is associated with hepatocellular senescence and correlates with mortality in patients with alcohol-associated hepatitis
    Article Snippet: Immunofluorescence Huh7 cells were washed in PBS, fixed with 4% paraformaldehyde (PFA), washed, and incubated overnight at 4oC with anti-human Ki67 (1:100, Ab16667, Abcam, Cambridge, United Kingdom) diluted in Dako REAL antibody Diluent (S2022, Dako). .. After washes with PBS, Huh7 were incubated with secondary donkey anti-rabbit antibody conjugated to Cy3 (711-165-152, Jackson ImmunoResearch Europe Ltd, Cambridge, United Kingdom) for 30 minutes at room temperature, washed, and mounted (H-1200, Vectashield® Mounting Medium with DAPI, Vector laboratories, Burlingame, CA, USA). .. Senescence-associated β-galactosidase Senescence-associated β-galactosidase (SA-β-gal) activity, which is defined as βgalactosidase activity detectable at pH 6.0, was detected using a commercial kit (9860, Senescence β-Galactosidase Staining Kit, Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer's instructions.

    Article Title: GDF15 is associated with hepatocellular senescence and correlates with mortality in patients with alcohol-associated hepatitis
    Article Snippet: Huh7 cells were washed in PBS, fixed with 4% paraformaldehyde (PFA), washed, and incubated overnight at 4oC with anti-human Ki67 (1:100, Ab16667, Abcam, Cambridge, United Kingdom) diluted in Dako REAL antibody Diluent (S2022, Dako). .. After washes with PBS, Huh7 were incubated with secondary donkey anti-rabbit antibody conjugated to Cy3 (711-165-152, Jackson ImmunoResearch Europe Ltd, Cambridge, United Kingdom) for 30 minutes at room temperature, washed, and mounted (H-1200, Vectashield® Mounting Medium with DAPI, Vector laboratories, Burlingame, CA, USA). .. Senescence-associated β-galactosidase Senescence-associated β-galactosidase (SA-β-gal) activity, which is defined as β- galactosidase activity detectable at pH 6.0, was detected using a commercial kit (9860, 6 Senescence β-Galactosidase Staining Kit, Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer's instructions.

    Labeling:

    Article Title: Peptidoglycan derived from Lacticaseibacillus rhamnosus and Lactobacillus acidophilus suppress TLR2/1-mediated inflammation in bovine endometrial epithelial cells
    Article Snippet: .. The primary antibodies were coated in the cell (TNF, PTGES, and TLR2) for labeling ( ) in a humid chamber at 4°C for 24 h. After washing the cells with PBS for five times and then incubating with Alexa Flour conjugated secondary antibody ( ) for 1 h at 4°C, the cells were washed with PBS six times, and then a VECTASHIELD mounting medium containing DAPI was used (H-1200, Vector Laboratories, CA, USA). .. Finally, the sections were observed under a fluorescence microscope (BZ-X800, Keyence) to visualize the distribution of TNF (normal mouse IgG replaced the primary antibody in the IgG control), PTGES, and TLR2 (normal rabbit IgG replaced the primary antibody in the IgG control) proteins in the BEECs.

    Recombinant:

    Article Title: The NLRP3 inflammasome in microglia regulates repetitive behavior by modulating NMDA glutamate receptor functions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal Bovine Serum (FBS) WELGENE Cat#: PK004-01 Penicillin/Streptomycin ThermoFisher Scientific Cat#: 15140122 PEI MAX Polyscience Cat#: 24765-1 Lipofectamine LTX Reagent with PLUSTM Reagent ThermoFisher Scientific Cat#: 15338100 HBSS (Hanks’ Balanced Salt Solution) ThermoFisher Cat#: 14065056 Papain Worthington Cat#: LS003126 Neurobasal medium ThermoFisher Scientific Cat#: 21103049 B-27 supplement (50X) ThermoFisher Scientific Cat#: 17504-044 GlutaMax Supplement ThermoFisher Scientific Cat#: 35050061 Sodium pyruvate ThermoFisher Scientific Cat#: 11360070 DMEM/F-12 Cytiva Cat#: SH30023.01 4-hydroxytamoxifen Sigma Cat#: H7904 Poly-D-lysine hydrobromide Sigma Cat#: P0899 Paraformaldehyde Sigma Cat#: 158127 37% formaldehyde Biosesang Cat#: F1012 Triton X-100 Sigma Cat#: T8787 Bovine serum albumin Sigma Cat#: A7906 Horse serum Gibco Cat#: 16050122 Vectashield mounting medium Vector Laboratories Cat#: H-1200, H-1000 DNaseI Sigma Cat#: AMPD1 human recombinant IL-1β This study N/A human recombinant IL-1RA This study N/A Poly(ethylene glycol); PEG Sigma Cat #: P5413 Chloroform Millipore Cat#: 102445 TRIzol reagent Ambicon Cat#: BRL-15596-026 ReverTra Ace-α kit TOYOBO Cat#: TOFSQ-301 TB green qPCR master mix Takara Cat#: RR420A DL-2-amino-5-phosphonopentanoic acid (DL-APV) Sigma Cat#: A5282 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) Sigma Cat#: C127 Picrotoxin Tocris Cat#: 1128 Tetrodoxin Tocris Cat#: 1069 Critical commercial assays mouse IL-1β DuoSet ELISA kit R&D Systems Cat#: DY401 DuoSet ELISA Ancillary Reagent Kit R&D Systems Cat#: DY008 Proteome Profiler Mouse Cytokine Array Kit R&D Systems Cat#: ARY006 In Situ Cell Death Detection Kit, TMR red Roche Cat#: 12156792910 Experimental models: cell lines HEK293T cells ATCC Cat#: CRL-3216 Cultured neuronal cells N/A N/A Cultured microglial cells N/A N/A Experimental models: organisms/strains Mouse: Nlrp3D301NneoR The Jackson Laboratory Cat#: 017971 Mouse: Cx3cr1-CreERT2 The Jackson Laboratory Cat#: 021160 Rat: E18 pregnant females (Sprague-Dawley) Daehan Biolink N/A Recombinant DNA pAAV-CW3SL:hSyn-PSD95-mCherry-eGFP (ExPost) Lee et al.26 N/A pAAV-CW3SL:hSyn-gephyrin-mCherry-eGFP (InhiPost) Lee et al.26 N/A (Continued on next page) Cell Reports 44, 115656, May 27, 2025 15 .. The Nlrp3D301N (D301N mutant KI) mouse (#017971) and Cx3cr1-CreERT2 mouse (#021160) were purchased from Jackson Laboratories.

    Real-time Polymerase Chain Reaction:

    Article Title: The NLRP3 inflammasome in microglia regulates repetitive behavior by modulating NMDA glutamate receptor functions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal Bovine Serum (FBS) WELGENE Cat#: PK004-01 Penicillin/Streptomycin ThermoFisher Scientific Cat#: 15140122 PEI MAX Polyscience Cat#: 24765-1 Lipofectamine LTX Reagent with PLUSTM Reagent ThermoFisher Scientific Cat#: 15338100 HBSS (Hanks’ Balanced Salt Solution) ThermoFisher Cat#: 14065056 Papain Worthington Cat#: LS003126 Neurobasal medium ThermoFisher Scientific Cat#: 21103049 B-27 supplement (50X) ThermoFisher Scientific Cat#: 17504-044 GlutaMax Supplement ThermoFisher Scientific Cat#: 35050061 Sodium pyruvate ThermoFisher Scientific Cat#: 11360070 DMEM/F-12 Cytiva Cat#: SH30023.01 4-hydroxytamoxifen Sigma Cat#: H7904 Poly-D-lysine hydrobromide Sigma Cat#: P0899 Paraformaldehyde Sigma Cat#: 158127 37% formaldehyde Biosesang Cat#: F1012 Triton X-100 Sigma Cat#: T8787 Bovine serum albumin Sigma Cat#: A7906 Horse serum Gibco Cat#: 16050122 Vectashield mounting medium Vector Laboratories Cat#: H-1200, H-1000 DNaseI Sigma Cat#: AMPD1 human recombinant IL-1β This study N/A human recombinant IL-1RA This study N/A Poly(ethylene glycol); PEG Sigma Cat #: P5413 Chloroform Millipore Cat#: 102445 TRIzol reagent Ambicon Cat#: BRL-15596-026 ReverTra Ace-α kit TOYOBO Cat#: TOFSQ-301 TB green qPCR master mix Takara Cat#: RR420A DL-2-amino-5-phosphonopentanoic acid (DL-APV) Sigma Cat#: A5282 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) Sigma Cat#: C127 Picrotoxin Tocris Cat#: 1128 Tetrodoxin Tocris Cat#: 1069 Critical commercial assays mouse IL-1β DuoSet ELISA kit R&D Systems Cat#: DY401 DuoSet ELISA Ancillary Reagent Kit R&D Systems Cat#: DY008 Proteome Profiler Mouse Cytokine Array Kit R&D Systems Cat#: ARY006 In Situ Cell Death Detection Kit, TMR red Roche Cat#: 12156792910 Experimental models: cell lines HEK293T cells ATCC Cat#: CRL-3216 Cultured neuronal cells N/A N/A Cultured microglial cells N/A N/A Experimental models: organisms/strains Mouse: Nlrp3D301NneoR The Jackson Laboratory Cat#: 017971 Mouse: Cx3cr1-CreERT2 The Jackson Laboratory Cat#: 021160 Rat: E18 pregnant females (Sprague-Dawley) Daehan Biolink N/A Recombinant DNA pAAV-CW3SL:hSyn-PSD95-mCherry-eGFP (ExPost) Lee et al.26 N/A pAAV-CW3SL:hSyn-gephyrin-mCherry-eGFP (InhiPost) Lee et al.26 N/A (Continued on next page) Cell Reports 44, 115656, May 27, 2025 15 .. The Nlrp3D301N (D301N mutant KI) mouse (#017971) and Cx3cr1-CreERT2 mouse (#021160) were purchased from Jackson Laboratories.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The NLRP3 inflammasome in microglia regulates repetitive behavior by modulating NMDA glutamate receptor functions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal Bovine Serum (FBS) WELGENE Cat#: PK004-01 Penicillin/Streptomycin ThermoFisher Scientific Cat#: 15140122 PEI MAX Polyscience Cat#: 24765-1 Lipofectamine LTX Reagent with PLUSTM Reagent ThermoFisher Scientific Cat#: 15338100 HBSS (Hanks’ Balanced Salt Solution) ThermoFisher Cat#: 14065056 Papain Worthington Cat#: LS003126 Neurobasal medium ThermoFisher Scientific Cat#: 21103049 B-27 supplement (50X) ThermoFisher Scientific Cat#: 17504-044 GlutaMax Supplement ThermoFisher Scientific Cat#: 35050061 Sodium pyruvate ThermoFisher Scientific Cat#: 11360070 DMEM/F-12 Cytiva Cat#: SH30023.01 4-hydroxytamoxifen Sigma Cat#: H7904 Poly-D-lysine hydrobromide Sigma Cat#: P0899 Paraformaldehyde Sigma Cat#: 158127 37% formaldehyde Biosesang Cat#: F1012 Triton X-100 Sigma Cat#: T8787 Bovine serum albumin Sigma Cat#: A7906 Horse serum Gibco Cat#: 16050122 Vectashield mounting medium Vector Laboratories Cat#: H-1200, H-1000 DNaseI Sigma Cat#: AMPD1 human recombinant IL-1β This study N/A human recombinant IL-1RA This study N/A Poly(ethylene glycol); PEG Sigma Cat #: P5413 Chloroform Millipore Cat#: 102445 TRIzol reagent Ambicon Cat#: BRL-15596-026 ReverTra Ace-α kit TOYOBO Cat#: TOFSQ-301 TB green qPCR master mix Takara Cat#: RR420A DL-2-amino-5-phosphonopentanoic acid (DL-APV) Sigma Cat#: A5282 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) Sigma Cat#: C127 Picrotoxin Tocris Cat#: 1128 Tetrodoxin Tocris Cat#: 1069 Critical commercial assays mouse IL-1β DuoSet ELISA kit R&D Systems Cat#: DY401 DuoSet ELISA Ancillary Reagent Kit R&D Systems Cat#: DY008 Proteome Profiler Mouse Cytokine Array Kit R&D Systems Cat#: ARY006 In Situ Cell Death Detection Kit, TMR red Roche Cat#: 12156792910 Experimental models: cell lines HEK293T cells ATCC Cat#: CRL-3216 Cultured neuronal cells N/A N/A Cultured microglial cells N/A N/A Experimental models: organisms/strains Mouse: Nlrp3D301NneoR The Jackson Laboratory Cat#: 017971 Mouse: Cx3cr1-CreERT2 The Jackson Laboratory Cat#: 021160 Rat: E18 pregnant females (Sprague-Dawley) Daehan Biolink N/A Recombinant DNA pAAV-CW3SL:hSyn-PSD95-mCherry-eGFP (ExPost) Lee et al.26 N/A pAAV-CW3SL:hSyn-gephyrin-mCherry-eGFP (InhiPost) Lee et al.26 N/A (Continued on next page) Cell Reports 44, 115656, May 27, 2025 15 .. The Nlrp3D301N (D301N mutant KI) mouse (#017971) and Cx3cr1-CreERT2 mouse (#021160) were purchased from Jackson Laboratories.

    In Situ:

    Article Title: The NLRP3 inflammasome in microglia regulates repetitive behavior by modulating NMDA glutamate receptor functions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal Bovine Serum (FBS) WELGENE Cat#: PK004-01 Penicillin/Streptomycin ThermoFisher Scientific Cat#: 15140122 PEI MAX Polyscience Cat#: 24765-1 Lipofectamine LTX Reagent with PLUSTM Reagent ThermoFisher Scientific Cat#: 15338100 HBSS (Hanks’ Balanced Salt Solution) ThermoFisher Cat#: 14065056 Papain Worthington Cat#: LS003126 Neurobasal medium ThermoFisher Scientific Cat#: 21103049 B-27 supplement (50X) ThermoFisher Scientific Cat#: 17504-044 GlutaMax Supplement ThermoFisher Scientific Cat#: 35050061 Sodium pyruvate ThermoFisher Scientific Cat#: 11360070 DMEM/F-12 Cytiva Cat#: SH30023.01 4-hydroxytamoxifen Sigma Cat#: H7904 Poly-D-lysine hydrobromide Sigma Cat#: P0899 Paraformaldehyde Sigma Cat#: 158127 37% formaldehyde Biosesang Cat#: F1012 Triton X-100 Sigma Cat#: T8787 Bovine serum albumin Sigma Cat#: A7906 Horse serum Gibco Cat#: 16050122 Vectashield mounting medium Vector Laboratories Cat#: H-1200, H-1000 DNaseI Sigma Cat#: AMPD1 human recombinant IL-1β This study N/A human recombinant IL-1RA This study N/A Poly(ethylene glycol); PEG Sigma Cat #: P5413 Chloroform Millipore Cat#: 102445 TRIzol reagent Ambicon Cat#: BRL-15596-026 ReverTra Ace-α kit TOYOBO Cat#: TOFSQ-301 TB green qPCR master mix Takara Cat#: RR420A DL-2-amino-5-phosphonopentanoic acid (DL-APV) Sigma Cat#: A5282 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) Sigma Cat#: C127 Picrotoxin Tocris Cat#: 1128 Tetrodoxin Tocris Cat#: 1069 Critical commercial assays mouse IL-1β DuoSet ELISA kit R&D Systems Cat#: DY401 DuoSet ELISA Ancillary Reagent Kit R&D Systems Cat#: DY008 Proteome Profiler Mouse Cytokine Array Kit R&D Systems Cat#: ARY006 In Situ Cell Death Detection Kit, TMR red Roche Cat#: 12156792910 Experimental models: cell lines HEK293T cells ATCC Cat#: CRL-3216 Cultured neuronal cells N/A N/A Cultured microglial cells N/A N/A Experimental models: organisms/strains Mouse: Nlrp3D301NneoR The Jackson Laboratory Cat#: 017971 Mouse: Cx3cr1-CreERT2 The Jackson Laboratory Cat#: 021160 Rat: E18 pregnant females (Sprague-Dawley) Daehan Biolink N/A Recombinant DNA pAAV-CW3SL:hSyn-PSD95-mCherry-eGFP (ExPost) Lee et al.26 N/A pAAV-CW3SL:hSyn-gephyrin-mCherry-eGFP (InhiPost) Lee et al.26 N/A (Continued on next page) Cell Reports 44, 115656, May 27, 2025 15 .. The Nlrp3D301N (D301N mutant KI) mouse (#017971) and Cx3cr1-CreERT2 mouse (#021160) were purchased from Jackson Laboratories.

    Cell Culture:

    Article Title: The NLRP3 inflammasome in microglia regulates repetitive behavior by modulating NMDA glutamate receptor functions.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal Bovine Serum (FBS) WELGENE Cat#: PK004-01 Penicillin/Streptomycin ThermoFisher Scientific Cat#: 15140122 PEI MAX Polyscience Cat#: 24765-1 Lipofectamine LTX Reagent with PLUSTM Reagent ThermoFisher Scientific Cat#: 15338100 HBSS (Hanks’ Balanced Salt Solution) ThermoFisher Cat#: 14065056 Papain Worthington Cat#: LS003126 Neurobasal medium ThermoFisher Scientific Cat#: 21103049 B-27 supplement (50X) ThermoFisher Scientific Cat#: 17504-044 GlutaMax Supplement ThermoFisher Scientific Cat#: 35050061 Sodium pyruvate ThermoFisher Scientific Cat#: 11360070 DMEM/F-12 Cytiva Cat#: SH30023.01 4-hydroxytamoxifen Sigma Cat#: H7904 Poly-D-lysine hydrobromide Sigma Cat#: P0899 Paraformaldehyde Sigma Cat#: 158127 37% formaldehyde Biosesang Cat#: F1012 Triton X-100 Sigma Cat#: T8787 Bovine serum albumin Sigma Cat#: A7906 Horse serum Gibco Cat#: 16050122 Vectashield mounting medium Vector Laboratories Cat#: H-1200, H-1000 DNaseI Sigma Cat#: AMPD1 human recombinant IL-1β This study N/A human recombinant IL-1RA This study N/A Poly(ethylene glycol); PEG Sigma Cat #: P5413 Chloroform Millipore Cat#: 102445 TRIzol reagent Ambicon Cat#: BRL-15596-026 ReverTra Ace-α kit TOYOBO Cat#: TOFSQ-301 TB green qPCR master mix Takara Cat#: RR420A DL-2-amino-5-phosphonopentanoic acid (DL-APV) Sigma Cat#: A5282 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) Sigma Cat#: C127 Picrotoxin Tocris Cat#: 1128 Tetrodoxin Tocris Cat#: 1069 Critical commercial assays mouse IL-1β DuoSet ELISA kit R&D Systems Cat#: DY401 DuoSet ELISA Ancillary Reagent Kit R&D Systems Cat#: DY008 Proteome Profiler Mouse Cytokine Array Kit R&D Systems Cat#: ARY006 In Situ Cell Death Detection Kit, TMR red Roche Cat#: 12156792910 Experimental models: cell lines HEK293T cells ATCC Cat#: CRL-3216 Cultured neuronal cells N/A N/A Cultured microglial cells N/A N/A Experimental models: organisms/strains Mouse: Nlrp3D301NneoR The Jackson Laboratory Cat#: 017971 Mouse: Cx3cr1-CreERT2 The Jackson Laboratory Cat#: 021160 Rat: E18 pregnant females (Sprague-Dawley) Daehan Biolink N/A Recombinant DNA pAAV-CW3SL:hSyn-PSD95-mCherry-eGFP (ExPost) Lee et al.26 N/A pAAV-CW3SL:hSyn-gephyrin-mCherry-eGFP (InhiPost) Lee et al.26 N/A (Continued on next page) Cell Reports 44, 115656, May 27, 2025 15 .. The Nlrp3D301N (D301N mutant KI) mouse (#017971) and Cx3cr1-CreERT2 mouse (#021160) were purchased from Jackson Laboratories.



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    vectashield antifade mounting medium with dapi  (Vector Laboratories)
    98
    Vector Laboratories vectashield antifade mounting medium with dapi
    Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h+1200/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/custom%40h-1200%4042668564
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