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gsmtx 4  (MedChemExpress)


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    Structured Review

    MedChemExpress gsmtx 4
    Typical calcium signature triggered by the addition of 10 µM Srf in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.
    Gsmtx 4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gsmtx+4/GsMTx4/pmc13197228-255-16-19
    Average 97 stars, based on 109 article reviews
    gsmtx 4 - by Bioz Stars, 2026-11
    97/100 stars

    Images

    1) Product Images from "Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis"

    Article Title: Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis

    Journal: Nature Plants

    doi: 10.1038/s41477-026-02270-3

    Typical calcium signature triggered by the addition of 10 µM Srf in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.
    Figure Legend Snippet: Typical calcium signature triggered by the addition of 10 µM Srf in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.

    Techniques Used:

    Typical calcium signature triggered by the addition of MAMPs (150 mg ml −1 CO8) in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.
    Figure Legend Snippet: Typical calcium signature triggered by the addition of MAMPs (150 mg ml −1 CO8) in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.

    Techniques Used:

    a , [Ca 2+ ] cyt levels in Col-0 AEQ protoplasts upon Srf (10 µM) or mock (WI Ca ), with or without pre-incubation with the mechanosensitive channel blocker GsMTX-4 (+Gx). n = 20 from four independent experiments. b , [Ca 2+ ] cyt response measured with the Fluo4-AM probe in Col-0 protoplasts treated with Srf (10 µM) or mock (WI Ca ), with or without pre-incubation with GsMTX-4 ( + Gx). Mean ± s.d., data are from four independent experiments with total n indicated. c , [ROS] intra accumulation in Col-0 roots 30 min after Srf (10 µM) or mock (water) treatment, in presence or absence of GsMTX-4 ( + Gx). Data are expressed as fold increase in DCFH-DA fluorescence compared to mock. Mean ± s.d., data are from three independent experiments with total n indicated. d , [Ca 2+ ] cyt signal in Col-0 AEQ protoplasts following Srf (10 µM) or mock (WI Ca ) treatment with or without pre-incubation with the calcium channel blocker LaCl 3 ( + La). Data are from four independent experiments with total n indicated. e , Calcium influx in Col-0 protoplasts measured via Fluo4-AM fluorescence after Srf (10 µM) or mock (WI Ca ) treatment, with or without LaCl 3 pre-treatment. Data are normalized fluorescence increases. Mean ± s.d., data are from at least two independent experiments with total n indicated. f , [Ca 2+ ] cyt signal in UBQ10::GCaMP3 roots treated with Srf (10 µM) or flg22 (1 µM) as MAMP, with ( + La) or without LaCl 3 pre-treatment. Signal quantified as fluorescence increase. Mean ± s.d., total n indicated. P -values from unpaired two-tailed t-test with Welch’s correction. In a and d , data show the average of luminescence (L/L max ) between 1.5 and 4 min post-treatment with Srf. Box plots show medians, quartiles, and ranges. Letters represent statistically different groups ( P < 0.05, two-way ANOVA with Bonferroni’s post-test). In b , c and e , letters indicate statistically different groups at P < 0.05, two-way ANOVA with Tukey’s post-test.
    Figure Legend Snippet: a , [Ca 2+ ] cyt levels in Col-0 AEQ protoplasts upon Srf (10 µM) or mock (WI Ca ), with or without pre-incubation with the mechanosensitive channel blocker GsMTX-4 (+Gx). n = 20 from four independent experiments. b , [Ca 2+ ] cyt response measured with the Fluo4-AM probe in Col-0 protoplasts treated with Srf (10 µM) or mock (WI Ca ), with or without pre-incubation with GsMTX-4 ( + Gx). Mean ± s.d., data are from four independent experiments with total n indicated. c , [ROS] intra accumulation in Col-0 roots 30 min after Srf (10 µM) or mock (water) treatment, in presence or absence of GsMTX-4 ( + Gx). Data are expressed as fold increase in DCFH-DA fluorescence compared to mock. Mean ± s.d., data are from three independent experiments with total n indicated. d , [Ca 2+ ] cyt signal in Col-0 AEQ protoplasts following Srf (10 µM) or mock (WI Ca ) treatment with or without pre-incubation with the calcium channel blocker LaCl 3 ( + La). Data are from four independent experiments with total n indicated. e , Calcium influx in Col-0 protoplasts measured via Fluo4-AM fluorescence after Srf (10 µM) or mock (WI Ca ) treatment, with or without LaCl 3 pre-treatment. Data are normalized fluorescence increases. Mean ± s.d., data are from at least two independent experiments with total n indicated. f , [Ca 2+ ] cyt signal in UBQ10::GCaMP3 roots treated with Srf (10 µM) or flg22 (1 µM) as MAMP, with ( + La) or without LaCl 3 pre-treatment. Signal quantified as fluorescence increase. Mean ± s.d., total n indicated. P -values from unpaired two-tailed t-test with Welch’s correction. In a and d , data show the average of luminescence (L/L max ) between 1.5 and 4 min post-treatment with Srf. Box plots show medians, quartiles, and ranges. Letters represent statistically different groups ( P < 0.05, two-way ANOVA with Bonferroni’s post-test). In b , c and e , letters indicate statistically different groups at P < 0.05, two-way ANOVA with Tukey’s post-test.

    Techniques Used: Incubation, Fluorescence, Two Tailed Test

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    Electron Microscopy:

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    CCK-8 Assay:

    Article Title: Surficial Pore Structure on Polyetheretherketone Implants Regulates Mechanical Properties to Promote Macrophage M2 Polarization.
    Article Snippet: The surficial micro−nanostructure on polyetheretherketone (PEEK) can increase the biocompatibility of biomaterials and promote macrophage M2 polarization to induce soft tissue integration.. However, because it is difficult to obtain a uniform and controllable surficial micro− nanostructure on PEEK, the regulation rules and underlying mechanisms for macrophage M2 polarization remain poorly understood.. In this study, we used a hot pressing technique to fabricate uniform submicrometer porous structures with sizes of 200, 500, and 800 nm on PEEK material.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Surficial Pore Structure on Polyetheretherketone Implants Regulates Mechanical Properties to Promote Macrophage M2 Polarization.
    Article Snippet: The surficial micro−nanostructure on polyetheretherketone (PEEK) can increase the biocompatibility of biomaterials and promote macrophage M2 polarization to induce soft tissue integration.. However, because it is difficult to obtain a uniform and controllable surficial micro− nanostructure on PEEK, the regulation rules and underlying mechanisms for macrophage M2 polarization remain poorly understood.. In this study, we used a hot pressing technique to fabricate uniform submicrometer porous structures with sizes of 200, 500, and 800 nm on PEEK material.

    Marker:

    Article Title: Surficial Pore Structure on Polyetheretherketone Implants Regulates Mechanical Properties to Promote Macrophage M2 Polarization.
    Article Snippet: The surficial micro−nanostructure on polyetheretherketone (PEEK) can increase the biocompatibility of biomaterials and promote macrophage M2 polarization to induce soft tissue integration.. However, because it is difficult to obtain a uniform and controllable surficial micro− nanostructure on PEEK, the regulation rules and underlying mechanisms for macrophage M2 polarization remain poorly understood.. In this study, we used a hot pressing technique to fabricate uniform submicrometer porous structures with sizes of 200, 500, and 800 nm on PEEK material.

    Western Blot:

    Article Title: Surficial Pore Structure on Polyetheretherketone Implants Regulates Mechanical Properties to Promote Macrophage M2 Polarization.
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    Tissue:

    Article Title: Surficial Pore Structure on Polyetheretherketone Implants Regulates Mechanical Properties to Promote Macrophage M2 Polarization.
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    Saline:

    Article Title: GsMTx‐4 inhibits the exercise pressor reflex and the muscle mechanoreflex primarily through TRPC inhibition
    Article Snippet: .. GsMTx-4 (1mg;MedChemExpress) and SAR7334 hydrochloride (5mg;MedChemExpress) were dissolved in 0.9% saline. ..

    Article Title: GsMTx‐4 inhibits the exercise pressor reflex and the muscle mechanoreflex primarily through TRPC inhibition
    Article Snippet: .. GsMTx‐4 (1 mg; MedChemExpress) and SAR7334 hydrochloride (5 mg; MedChemExpress) were dissolved in 0.9% saline. ..

    other:

    Article Title: Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infection
    Article Snippet: Additional inhibitors, including S62845 (HY-100741), emricasan (HY-10396), 2-APB (B6643), SKF96365 (HY-100001), HC067047 (HY-100208), Pyr3 (HY-108465), JNJ-28583113 (HY-149143), 9-phenanthrol (HY-108457), carvacrol (HY-N0711), GsMTx-4 (HY-P1410), probenecid (HY-B0545), GSK1016790A (HY-19608), GSK1702934A (HY-111098), bilirubin (HY-N0323), Yoda1 (HY-18723) and NTB (HY-101302) were obtained from MedChemExpress.

    Article Title: Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infection.
    Article Snippet: Additional inhibitors, including S62845 (HY-100741), emricasan (HY-10396), 2-APB (B6643), SKF96365 (HY-100001), HC067047 (HY-100208), Pyr3 (HY-108465), JNJ-28583113 (HY-149143), 9-phenanthrol (HY108457), carvacrol (HY-N0711), GsMTx-4 (HY-P1410), probenecid (HY-B0545), GSK1016790A (HY-19608), GSK1702934A (HY-111098), bilirubin (HY-N0323), Yoda1 (HY-18723) and NTB (HY-101302) were obtained from MedChemExpress.



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    Alomone Labs gsmtx4
    Intracellular Ca 2+ is required for Piezo1‐induced EV release in stably transfected HEK293 cells. (a) Piezo1 overexpression was confirmed by Western blotting. (b) Fluorescence microscopy showed co‐expression of PalmReNL (red) and Piezo1/GFP (green) in HEK293 cells. Scale bars, 50 µm. (c) Yoda1‐induced production of PalmReNL‐labelled EVs was quantified by measuring bioluminescence in culture supernatants. Reporter cells were treated with 30 µM Yoda1 for 6 h before supernatant collection. (d) Piezo1/PalmReNL‐expressing HEK293 cells were treated with 30 µM Yoda1 in the presence or absence of 2.5 µM <t>GsMTx4.</t> EV release was assessed by measuring PalmReNL‐associated bioluminescence in the culture supernatant after treatment. (e) Bioluminescence analysis of PalmReNL‐EVs released from Piezo1/PalmReNL co‐expressing cells under Ca 2+ ‐containing or Ca 2+ ‐free conditions following Piezo1 activation with 30 µM Yoda1 for 6 h. (f) Effect of intracellular Ca 2+ chelation on Piezo1‐induced EV release. Reporter cells were pretreated with 5–20 µM BAPTA‐AM for 10 h, followed by co‐treatment with 30 µM Yoda1 and BAPTA‐AM for an additional 6 h. Error bars represent SD ( n = 4).
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    MedChemExpress gsmtx 4
    Typical calcium signature triggered by the addition of 10 µM Srf in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.
    Gsmtx 4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gsmtx+4/GsMTx4/pmc13197228-255-16-19
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    gsmtx 4 - by Bioz Stars, 2026-11
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      Buy from Supplier

    Image Search Results


    Intracellular Ca 2+ is required for Piezo1‐induced EV release in stably transfected HEK293 cells. (a) Piezo1 overexpression was confirmed by Western blotting. (b) Fluorescence microscopy showed co‐expression of PalmReNL (red) and Piezo1/GFP (green) in HEK293 cells. Scale bars, 50 µm. (c) Yoda1‐induced production of PalmReNL‐labelled EVs was quantified by measuring bioluminescence in culture supernatants. Reporter cells were treated with 30 µM Yoda1 for 6 h before supernatant collection. (d) Piezo1/PalmReNL‐expressing HEK293 cells were treated with 30 µM Yoda1 in the presence or absence of 2.5 µM GsMTx4. EV release was assessed by measuring PalmReNL‐associated bioluminescence in the culture supernatant after treatment. (e) Bioluminescence analysis of PalmReNL‐EVs released from Piezo1/PalmReNL co‐expressing cells under Ca 2+ ‐containing or Ca 2+ ‐free conditions following Piezo1 activation with 30 µM Yoda1 for 6 h. (f) Effect of intracellular Ca 2+ chelation on Piezo1‐induced EV release. Reporter cells were pretreated with 5–20 µM BAPTA‐AM for 10 h, followed by co‐treatment with 30 µM Yoda1 and BAPTA‐AM for an additional 6 h. Error bars represent SD ( n = 4).

    Journal: Journal of Extracellular Biology

    Article Title: Engineering Piezo1‐Mediated Calcium Signalling for Enhanced Extracellular Vesicle Biogenesis and Cargo Loading

    doi: 10.1002/jex2.70182

    Figure Lengend Snippet: Intracellular Ca 2+ is required for Piezo1‐induced EV release in stably transfected HEK293 cells. (a) Piezo1 overexpression was confirmed by Western blotting. (b) Fluorescence microscopy showed co‐expression of PalmReNL (red) and Piezo1/GFP (green) in HEK293 cells. Scale bars, 50 µm. (c) Yoda1‐induced production of PalmReNL‐labelled EVs was quantified by measuring bioluminescence in culture supernatants. Reporter cells were treated with 30 µM Yoda1 for 6 h before supernatant collection. (d) Piezo1/PalmReNL‐expressing HEK293 cells were treated with 30 µM Yoda1 in the presence or absence of 2.5 µM GsMTx4. EV release was assessed by measuring PalmReNL‐associated bioluminescence in the culture supernatant after treatment. (e) Bioluminescence analysis of PalmReNL‐EVs released from Piezo1/PalmReNL co‐expressing cells under Ca 2+ ‐containing or Ca 2+ ‐free conditions following Piezo1 activation with 30 µM Yoda1 for 6 h. (f) Effect of intracellular Ca 2+ chelation on Piezo1‐induced EV release. Reporter cells were pretreated with 5–20 µM BAPTA‐AM for 10 h, followed by co‐treatment with 30 µM Yoda1 and BAPTA‐AM for an additional 6 h. Error bars represent SD ( n = 4).

    Article Snippet: GsMTx4 (STG‐100, Alomone Labs) was dissolved in water at 250 μM.

    Techniques: Stable Transfection, Transfection, Over Expression, Western Blot, Fluorescence, Microscopy, Expressing, Analysis, Activation Assay

    Typical calcium signature triggered by the addition of 10 µM Srf in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.

    Journal: Nature Plants

    Article Title: Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis

    doi: 10.1038/s41477-026-02270-3

    Figure Lengend Snippet: Typical calcium signature triggered by the addition of 10 µM Srf in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.

    Article Snippet: Experiments with the channel blockers included an additional step where LaCl 3 (10 mM; Sigma-Aldrich) or GsMTX-4 (7.5 μM; MedChemExpress) was added 15 min before treatments.

    Techniques:

    Typical calcium signature triggered by the addition of MAMPs (150 mg ml −1 CO8) in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.

    Journal: Nature Plants

    Article Title: Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis

    doi: 10.1038/s41477-026-02270-3

    Figure Lengend Snippet: Typical calcium signature triggered by the addition of MAMPs (150 mg ml −1 CO8) in the roots of the Arabidopsis UBQ10 :: GCaMP3 reporter line pretreated with the mechanosensitive channel blocker GsMTX4.

    Article Snippet: Experiments with the channel blockers included an additional step where LaCl 3 (10 mM; Sigma-Aldrich) or GsMTX-4 (7.5 μM; MedChemExpress) was added 15 min before treatments.

    Techniques:

    a , [Ca 2+ ] cyt levels in Col-0 AEQ protoplasts upon Srf (10 µM) or mock (WI Ca ), with or without pre-incubation with the mechanosensitive channel blocker GsMTX-4 (+Gx). n = 20 from four independent experiments. b , [Ca 2+ ] cyt response measured with the Fluo4-AM probe in Col-0 protoplasts treated with Srf (10 µM) or mock (WI Ca ), with or without pre-incubation with GsMTX-4 ( + Gx). Mean ± s.d., data are from four independent experiments with total n indicated. c , [ROS] intra accumulation in Col-0 roots 30 min after Srf (10 µM) or mock (water) treatment, in presence or absence of GsMTX-4 ( + Gx). Data are expressed as fold increase in DCFH-DA fluorescence compared to mock. Mean ± s.d., data are from three independent experiments with total n indicated. d , [Ca 2+ ] cyt signal in Col-0 AEQ protoplasts following Srf (10 µM) or mock (WI Ca ) treatment with or without pre-incubation with the calcium channel blocker LaCl 3 ( + La). Data are from four independent experiments with total n indicated. e , Calcium influx in Col-0 protoplasts measured via Fluo4-AM fluorescence after Srf (10 µM) or mock (WI Ca ) treatment, with or without LaCl 3 pre-treatment. Data are normalized fluorescence increases. Mean ± s.d., data are from at least two independent experiments with total n indicated. f , [Ca 2+ ] cyt signal in UBQ10::GCaMP3 roots treated with Srf (10 µM) or flg22 (1 µM) as MAMP, with ( + La) or without LaCl 3 pre-treatment. Signal quantified as fluorescence increase. Mean ± s.d., total n indicated. P -values from unpaired two-tailed t-test with Welch’s correction. In a and d , data show the average of luminescence (L/L max ) between 1.5 and 4 min post-treatment with Srf. Box plots show medians, quartiles, and ranges. Letters represent statistically different groups ( P < 0.05, two-way ANOVA with Bonferroni’s post-test). In b , c and e , letters indicate statistically different groups at P < 0.05, two-way ANOVA with Tukey’s post-test.

    Journal: Nature Plants

    Article Title: Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis

    doi: 10.1038/s41477-026-02270-3

    Figure Lengend Snippet: a , [Ca 2+ ] cyt levels in Col-0 AEQ protoplasts upon Srf (10 µM) or mock (WI Ca ), with or without pre-incubation with the mechanosensitive channel blocker GsMTX-4 (+Gx). n = 20 from four independent experiments. b , [Ca 2+ ] cyt response measured with the Fluo4-AM probe in Col-0 protoplasts treated with Srf (10 µM) or mock (WI Ca ), with or without pre-incubation with GsMTX-4 ( + Gx). Mean ± s.d., data are from four independent experiments with total n indicated. c , [ROS] intra accumulation in Col-0 roots 30 min after Srf (10 µM) or mock (water) treatment, in presence or absence of GsMTX-4 ( + Gx). Data are expressed as fold increase in DCFH-DA fluorescence compared to mock. Mean ± s.d., data are from three independent experiments with total n indicated. d , [Ca 2+ ] cyt signal in Col-0 AEQ protoplasts following Srf (10 µM) or mock (WI Ca ) treatment with or without pre-incubation with the calcium channel blocker LaCl 3 ( + La). Data are from four independent experiments with total n indicated. e , Calcium influx in Col-0 protoplasts measured via Fluo4-AM fluorescence after Srf (10 µM) or mock (WI Ca ) treatment, with or without LaCl 3 pre-treatment. Data are normalized fluorescence increases. Mean ± s.d., data are from at least two independent experiments with total n indicated. f , [Ca 2+ ] cyt signal in UBQ10::GCaMP3 roots treated with Srf (10 µM) or flg22 (1 µM) as MAMP, with ( + La) or without LaCl 3 pre-treatment. Signal quantified as fluorescence increase. Mean ± s.d., total n indicated. P -values from unpaired two-tailed t-test with Welch’s correction. In a and d , data show the average of luminescence (L/L max ) between 1.5 and 4 min post-treatment with Srf. Box plots show medians, quartiles, and ranges. Letters represent statistically different groups ( P < 0.05, two-way ANOVA with Bonferroni’s post-test). In b , c and e , letters indicate statistically different groups at P < 0.05, two-way ANOVA with Tukey’s post-test.

    Article Snippet: Experiments with the channel blockers included an additional step where LaCl 3 (10 mM; Sigma-Aldrich) or GsMTX-4 (7.5 μM; MedChemExpress) was added 15 min before treatments.

    Techniques: Incubation, Fluorescence, Two Tailed Test