fibroblast growth medium 2 media (PromoCell)

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PromoCell fibroblast growth medium 2 media
Fibroblast Growth Medium 2 Media, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/growth+media/Fibroblast+Growth+Medium+2/pm42230665-155-21-32
Average 96 stars, based on 162 article reviews

fibroblast growth medium 2 media - by Bioz Stars, 2026-09

96/100 stars

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Isolation:

Article Title: Polypeptides synthesized by common bacteria in the human gut improve rodent metabolism
Article Snippet: .. Human osteoblasts (Promocell, C-12720, lot number 445Z012.2, isolated from human femoral heads) were cultured in growth media (Promocell, C-27001, basal medium supplemented with 10% fetal calf serum) until 80% confluent and switched into differentiation media containing differentiation-promoting supplements of 10 mM β-glycerophosphate and 50 μg ml −1 ascorbic acid in α-MEM (Fisher Scientific, 15430584). ..

Article Title: Polypeptides synthesized by common bacteria in the human gut improve rodent metabolism
Article Snippet: .. Human skeletal myoblasts (PromoCell, C-12530, lot number 451Z031.15, isolated from human musculus pectoralis major) were cultured in growth media (PromoCell, C-23060, basal medium supplemented with 5% fetal calf serum, 50 μg ml −1 fetuin, 10 ng ml −1 epidermal growth factor, 1 ng ml −1 basic fibroblast growth factor, 10 μg ml −1 insulin, 0.4 μg ml −1 dexamethasone) until 80% confluent and switched into differentiation media (PromoCell, C-23061, basal medium supplemented with 10 μg ml −1 insulin) developed to induce the fusion of myoblasts to form myotubes with typical multinucleated syncytia. ..

Article Title: Polypeptides synthesized by common bacteria in the human gut improve rodent metabolism.
Article Snippet: .. Human skeletal myoblasts (PromoCell, C-12530, lot number 451Z031.15, isolated from human musculus pectoralis major) were cultured in growth media (PromoCell, C-23060, basal medium supplemented with 5% fetal calf serum, 50 μg ml−1 fetuin, 10 ng ml−1 epidermal growth factor, 1 ng ml−1 basic fibroblast growth factor, 10 μg ml−1 insulin, 0.4 μg ml−1 dexamethasone) until 80% confluent and switched into differentiation media (PromoCell, C-23061, basal medium supplemented with 10 μg ml−1 insulin) developed to induce the fusion of myoblasts to form myotubes with typical multinucleated syncytia. ..

Article Title: Polypeptides synthesized by common bacteria in the human gut improve rodent metabolism.
Article Snippet: .. Human osteoblasts (Promocell, C-12720, lot number 445Z012.2, isolated from human femoral heads) were cultured in growth media (Promocell, C-27001, basal medium supplemented with 10% fetal calf serum) until 80% confluent and switched into differentiation media containing differentiation-promoting supplements of 10 mM β-glycerophosphate and 50 μg ml−1 ascorbic acid in α-MEM (Fisher Scientific, 15430584). ..

Cell Culture:

Article Title: ratioPCR delivers precise real-time quantification from multi-template PCR via mechanistic bias correction
Article Snippet: .. Immortalized human myoblasts were obtained from the Institute of Myology, Paris, and were cultured in Growth Media (40% Skeletal Muscle Cell Growth Medium Kit (C-23160-PRO PromoCell), 40% Nutrient Mixture F-10 Ham medium (Sigma N6013), and 20% FBS (Gibco 10270 106) supplemented with 50 μg/mL gentamicin (Sigma G1272-100ML). .. For differentiation, the cells were plated in a 96-well plate at a density of 25’000 cells per well for two days in proliferation media (Skeletal Muscle Cell Differentiation Medium (C-23061-PRO PromoCell and 50 μg/mL gentamicin).

Article Title: Perfusion Bioreactor Culture Incorporating Mechanical Confinement Enhances Mesenchymal Stem Cell Extracellular Vesicle Production and Wound Healing Potential
Article Snippet: Human induced pluripotent stem cell-derived MSCs (iMSCs) and human bone marrow-derived MSCs (BMMSCs) were purchased from ATCC (ACS-7010, PCS-500-012) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Corning; 10-013-CV) supplemented with 10% fetal bovine serum (FBS) (Cytiva; SH30910.03), 1% penicillin-streptomycin (VWR; 45000-652), and 1% MEM non-essential amino acids (ThermoFisher Scientific; 11140050). .. Human umbilical vein endothelial cells (HUVECs) and human dermal microvascular endothelial cells (HDMECs) were purchased from PromoCell (C-12203, C-12212) and cultured in endothelial growth media (PromoCell; C-2212) with 1% penicillin-streptomycin in 0.1% gelatin-coated tissue culture flasks. .. Human primary epidermal keratinocytes were purchased from ATCC (PCS-200-011) and cultured in dermal cell basal media (ATCC; PCS-200-030) supplemented with ATCC’s Keratinocyte Growth Kit components (ATCC; PCS-200-040) and 0.1% penicillin-streptomycin.

Centrifugation:

Article Title: Persistent Fibrosis in Heart Failure With a Reduced Ejection Fraction Linked to Phenotypic Differences in Human Cardiac Fibroblast Populations
Article Snippet: Briefly, myocardium was weighed and then finely minced and incubated with successive changes of collagenase (Liberase Blendzyme 3, Roche No. 05401020001) over two hours with intermittent trituration until digestion was complete. .. Pooled collagenase fractions were placed in growth media (Fibroblast Growth Medium 2, Promocell No. C-23020, with 10% FBS and 1% antibiotics/antimycotics) and subjected to centrifugation at 1000rpm for 5 mins at RT. ..

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