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graphpad prism 2016  (GraphPad Software Inc)


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    GraphPad Software Inc graphpad prism 2016
    Graphpad Prism 2016, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/graphpad+prism+2016/graphpad+prism+2016/pm37531094-119-12-11
    Average 90 stars, based on 1 article reviews
    graphpad prism 2016 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: A Fluorogenic Disaccharide Substrate for α-Mannosidases Enables High-Throughput Screening and Identification of an Inhibitor of the GH92 Virulence Factor from Streptococcus pneumoniae .
    Article Snippet: Trimming of host glycans is a mechanism that is broadly employed by both commensal and pathogenic microflora to enable colonization.. Host glycan trimming by the opportunistic Gram-positive bacterium Streptococcus pneumoniae has been demonstrated to be an important mechanism of virulence.. While S. pneumoniae employs a multitude of glycan processing enzymes, the exo-mannosidase SpGH92 has been shown to be an important virulence factor.

    Article Title: Synthesis, conformational analysis and glycosidase inhibition of bicyclic nojirimycin C-glycosides based on an octahydrofuro[3,2-b]pyridine motif.
    Article Snippet: .. GraphPad Prism 2016 was used to calculate the IC50 values for each compound. hOGA: IC50 values for compounds against the human Protein OGlcNAcase (hOGA) enzyme, were determined by measuring the change in fluorescent signal corresponding to the rate of hydrolytic activity against the artificial substrate, Resorufin-N-acetyl-β-D-glucosaminide (synthesized by Sandeep Bhosale of the Vocadlo Lab). hOGA inhibition assays were performed in a buffer of 20 mM HEPES, 5 mM EDTA, 150 mM KCl, pH 7.1 and 0.2 nM [hOGA], in the presence or absence of various concentrations of compounds and at a fixed substrate concentration of 25 μM and 1% DMSO. ..

    Activity Assay:

    Article Title: Synthesis, conformational analysis and glycosidase inhibition of bicyclic nojirimycin C-glycosides based on an octahydrofuro[3,2-b]pyridine motif.
    Article Snippet: .. GraphPad Prism 2016 was used to calculate the IC50 values for each compound. hOGA: IC50 values for compounds against the human Protein OGlcNAcase (hOGA) enzyme, were determined by measuring the change in fluorescent signal corresponding to the rate of hydrolytic activity against the artificial substrate, Resorufin-N-acetyl-β-D-glucosaminide (synthesized by Sandeep Bhosale of the Vocadlo Lab). hOGA inhibition assays were performed in a buffer of 20 mM HEPES, 5 mM EDTA, 150 mM KCl, pH 7.1 and 0.2 nM [hOGA], in the presence or absence of various concentrations of compounds and at a fixed substrate concentration of 25 μM and 1% DMSO. ..

    Synthesized:

    Article Title: Synthesis, conformational analysis and glycosidase inhibition of bicyclic nojirimycin C-glycosides based on an octahydrofuro[3,2-b]pyridine motif.
    Article Snippet: .. GraphPad Prism 2016 was used to calculate the IC50 values for each compound. hOGA: IC50 values for compounds against the human Protein OGlcNAcase (hOGA) enzyme, were determined by measuring the change in fluorescent signal corresponding to the rate of hydrolytic activity against the artificial substrate, Resorufin-N-acetyl-β-D-glucosaminide (synthesized by Sandeep Bhosale of the Vocadlo Lab). hOGA inhibition assays were performed in a buffer of 20 mM HEPES, 5 mM EDTA, 150 mM KCl, pH 7.1 and 0.2 nM [hOGA], in the presence or absence of various concentrations of compounds and at a fixed substrate concentration of 25 μM and 1% DMSO. ..

    Inhibition:

    Article Title: Synthesis, conformational analysis and glycosidase inhibition of bicyclic nojirimycin C-glycosides based on an octahydrofuro[3,2-b]pyridine motif.
    Article Snippet: .. GraphPad Prism 2016 was used to calculate the IC50 values for each compound. hOGA: IC50 values for compounds against the human Protein OGlcNAcase (hOGA) enzyme, were determined by measuring the change in fluorescent signal corresponding to the rate of hydrolytic activity against the artificial substrate, Resorufin-N-acetyl-β-D-glucosaminide (synthesized by Sandeep Bhosale of the Vocadlo Lab). hOGA inhibition assays were performed in a buffer of 20 mM HEPES, 5 mM EDTA, 150 mM KCl, pH 7.1 and 0.2 nM [hOGA], in the presence or absence of various concentrations of compounds and at a fixed substrate concentration of 25 μM and 1% DMSO. ..

    Article Title: A Fluorogenic Disaccharide Substrate for α-Mannosidases Enables High-Throughput Screening and Identification of an Inhibitor of the GH92 Virulence Factor from Streptococcus pneumoniae .
    Article Snippet: .. GraphPad Prism 2016 was used to construct a Lineweaver-Burke plot to assign the inhibition type as competitive, then Ki was calculated to be 43 μM. .. Differential Scanning Fluorimetry was performed using an Applied Biosystems QuantStudio 3 RTPCR machine equipped with a 96-well heating block.

    other:

    Article Title: In vitro angiogenesis inhibition with selective compounds targeting the key glycolytic enzyme PFKFB3.
    Article Snippet: Statistical analysis and graphical presentations were performed using GraphPad Prism 2016. CRediT authorship contribution statement Designed the experiments: AA, SB.

    Construct:

    Article Title: A Fluorogenic Disaccharide Substrate for α-Mannosidases Enables High-Throughput Screening and Identification of an Inhibitor of the GH92 Virulence Factor from Streptococcus pneumoniae .
    Article Snippet: .. GraphPad Prism 2016 was used to construct a Lineweaver-Burke plot to assign the inhibition type as competitive, then Ki was calculated to be 43 μM. .. Differential Scanning Fluorimetry was performed using an Applied Biosystems QuantStudio 3 RTPCR machine equipped with a 96-well heating block.



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Immunity

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses

    doi: 10.1016/j.immuni.2020.11.003

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Techniques: Recombinant, Derivative Assay, Enzyme-linked Immunosorbent Assay, Sequencing, CRISPR, Plasmid Preparation, Expressing, Software, Single-cell Analysis