gradient-making station (Biocomp Instruments Inc)
90
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Biocomp Instruments Inc
gradient-making station
Gradient Making Station, supplied by Biocomp Instruments Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gradient-making+station/gradient+making+station/pmc12000384-102-0-3
Average 90 stars, based on 1 article reviews
Gradient Making Station, supplied by Biocomp Instruments Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gradient-making+station/gradient+making+station/pmc12000384-102-0-3
Average 90 stars, based on 1 article reviews
gradient-making station - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome"
Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome
Journal: The EMBO Journal
doi: 10.1038/s44318-025-00386-4
Figure Legend Snippet: Reagents and tools table
Techniques Used: Purification, Recombinant, Mutagenesis, Strep-tag, Clone Assay, FLAG-tag, Sequencing, Protein Purification, Protease Inhibitor, Software, Electron Microscopy, Staining, Chromatography
Related Articles
Purification:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Recombinant:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Mutagenesis:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Strep-tag:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Clone Assay:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a FLAG-tag:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Sequencing:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Protein Purification:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Protease Inhibitor:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Software:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Electron Microscopy:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Staining:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a Chromatography:Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top of an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol and 0.16% glutaraldehyde [Sigma]) in a SW40 Ti rotor 14 mL tube (Beckman) and gradients made using a gradient-making Article Title: Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork Article Snippet: For co-expression experiments, Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol) was layered on top an equal volume of freshly prepared Buffer B (Buffer A, except 30% v/v glycerol + 0.16% glutaraldehyde [Sigma]) in a 14 mL SW40-Ti tube (Beckman) and gradients made using a gradient-making Article Title: How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replication Article Snippet: Glycerol gradients were prepared as previously described : Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc) 2 ) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS 3 , ThermoFisher Scientific)) in a 2.2 mL TLS-55 tube (Beranek Laborgerate) and gradients made using a gradient-making Article Title: Integration of individualized and population-level molecular epidemiology data to model COVID-19 outcomes. Article Snippet: Glycerol gradients were prepared as previously described30: Buffer A (40 mM HEPES-NaOH, pH 7.5, 150 mM NaOAc, 0.5 mM TCEP, 10% v/v glycerol, 0.5 mM AMP-PNP and 3 mM Mg(OAc)2) was layered on top an equal volume of Buffer B (Buffer A, except 30% v/v glycerol, 0.16% glutaraldehyde [Sigma] and 2mM bis(sulfosuccinimidyl)suberate (BS3, ThermoFisher Scientific)) in a 2.2 mL Article Title: Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisome Article Snippet: Glycerol gradients were prepared as previously described (Jones et al, ): Article Title: DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans . Article Snippet: INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.. In humans, defects in replisome assembly are often associatedwith early cancer development and can lead to a form of microcephalic primordial dwarfismcalledMeier-Gorlin syndrome.. Eukaryotic replisome assembly is initiated by the assembly and activation of the 11-subunit helicase called CMG (CDC-45–MCM-2-7–GINS), around which the replisome forms. Article Title: Structure of a human replisome shows the organisation and interactions of a DNA replication machine Article Snippet: Gradients were prepared using a |