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gradient gel ipg strips  (Bio-Rad)


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    Bio-Rad gradient gel ipg strips
    Gradient Gel Ipg Strips, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gradient+gel+strips/ReadyStrip+IPG+Strips/pmc11854222-215-15-29
    Average 96 stars, based on 1584 article reviews
    gradient gel ipg strips - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Electrofocusing:

    Article Title: Ferulic acid prevents the injury-induced decrease of γ-enolase expression in brain tissue and HT22 cells
    Article Snippet: After centrifugation of the lysates at 16,000 g for 20 min at 4°C, the supernatants were collected, and the total protein concentrations were determined with the Bradford assay (Bio-Rad, Hercules, CA, USA). .. Then, 50 μg protein was mixed with rehydration solution, and isoelectric focusing (IEF) was performed for 13 h using immobilized pH gradient gel strips (IPG, pH 4-7, pH 6-9, 17 cm, Bio-Rad). .. Proteins were focused on Protean IEF Cells (Bio-Rad) at 20°C using the following conditions: 250 V (15 min), 10,000 V (3 h), and then a ramp from 10,000 to 50,000 V. After IEF, IPG strips were applied to gradient gels (7.5-17.5%) and electrophoresed at 10 mA/gel for 10 h using Protein-XI electrophoresis equipment (Bio-Rad) at 10°C.

    Article Title: Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling
    Article Snippet: .. 2D gel analysis used the Protean IEF Cell system with immobilized pH gradient gel strips (pH 3–10; Bio-Rad Laboratories) followed by denaturing 10% acrylamide gels. .. For MS analysis, proteins were stained with Colloidal blue (Invitrogen), excised from the gel, washed twice in 50% acetonitrile/H 2 O, and then frozen.

    Protein Concentration:

    Article Title: Translocation of Glycogen Synthase Kinase-3β (GSK-3β), a Trigger of Permeability Transition, Is Kinase Activity-dependent and Mediated by Interaction with Voltage-dependent Anion Channel 2 (VDAC2)
    Article Snippet: The protein samples were mixed with Rehydration Buffer (Bio-Rad) for isoelectrical focusing. .. The immobilized pH gradient gel strips (pH range, 3–10; 7 cm; Bio-Rad) were rehydrated in 125 μl of samples diluted with ReadyPrep Rehydration/Sample Buffer with a final protein concentration of 0.6 g/μl for 12 h at 20 °C. .. Isoelectric focusing was performed on the pre-rehydrated immobilized pH gradient gel strips at 20 °C in four steps: (i) 250 V for 15 min and (ii) 4000 V for 1 h in a linear mode where voltage increases are in a linear fashion to the set voltage followed by (iii) 4000 V for 3 h and (iv) 500 V for 24 h in a rapid mode where voltage is limited by the set current value using a Protean isoelectric focusing cell (Bio-Rad).

    Article Title: Translocation of Glycogen Synthase Kinase-3β (GSK-3β), a Trigger of Permeability Transition, Is Kinase Activity-dependent and Mediated by Interaction with Voltage-dependent Anion Channel 2 (VDAC2)
    Article Snippet: The protein samples were mixed with Rehydration Buffer (Bio-Rad) for isoelectrical focusing. .. The immobilized pH gradient gel strips (pH range, 3–10; 7 cm; Bio-Rad) were rehydrated in 125 l of samples diluted with ReadyPrep Rehydration/Sample Buffer with a final protein concentration of 0.6 g/ l for 12 h at 20 °C. .. Isoelectric focusing was performed on the pre-rehydrated immobilized pH gradient gel strips at 20 °C in four steps: (i) 250 V for 15 min and (ii) 4000 V for 1 h in a linear mode where voltage increases are in a linear fashion to the set voltage followed by (iii) 4000 V for 3 h and (iv) 500 V for 24 h in a rapid mode where voltage is limited by the set current value using a Protean isoelectric focusing cell (BioRad).

    Extraction:

    Article Title: DNA encoding
    Article Snippet: .. Immobilized pH gradient gel strips (11 cm, pH 3-10 gradient) were rehydrated in buffer (8M urea, 2% CHAPS, 2 mM TBP, 0.2% Bio-Lytes 3/10, and 0.001% bromophenol blue) containing 125 μg of T. gondii solubilized proteins from the extraction procedure described above at 50V for 12 hrs, followed by focusing at 5000V for 35,000 Vh using the BioRad IPG focusing cell. .. Strips containing focused proteins were soaked at room temperature in equilibration buffer (6M urea, 0.375 M Tris-HCl, pH 8.8, 2% SDS, 20% glycerol, 2% dithiothreitol) for 30 min and electrophoresed in the 2nd dimension on 4-12% gradient Bis-Tris gels (Invitrogen, Carlsbad, Calif.) in 50 mM MOPS-SDS running buffer (50 mM 3-N-morpholino propane sulfonic acid, 50 mM Tris base, 3.5 mM SDS, 1 mM EDTA, pH 7.7) at 150V for 75 min. All 2-D gel supplies, including 2-D analysis software, unless otherwise noted, were purchased from BioRad Laboratories (Hercules, Calif.).

    Two-Dimensional Gel Electrophoresis:

    Article Title: Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling
    Article Snippet: .. 2D gel analysis used the Protean IEF Cell system with immobilized pH gradient gel strips (pH 3–10; Bio-Rad Laboratories) followed by denaturing 10% acrylamide gels. .. For MS analysis, proteins were stained with Colloidal blue (Invitrogen), excised from the gel, washed twice in 50% acetonitrile/H 2 O, and then frozen.



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    Image Search Results


    Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.

    Journal: PLoS ONE

    Article Title: Proteomic Changes during B Cell Maturation: 2D-DIGE Approach

    doi: 10.1371/journal.pone.0077894

    Figure Lengend Snippet: Early pre-B 380 and IM-B Ramos (120 h time point) proteome samples were labelled with Cy5 and Cy3 dyes, respectively, and separated in a 2D-DIGE gel (nonlinear pH gradient 3–10) with internal standard sample labelled with Cy2 dye. Triple image overlay shows proteins highly expressed in Ramos (red) and 380 (green) samples in relative to internal standard sample (blue). Protein identities are indicated for those proteins with major changes in expression profile. IgM chains, ER and membrane bound vesicle proteins were highly expressed in Ramos cells, whereas cytoskeleton and immunity related proteins were expressed in 380 cells.

    Article Snippet: The IEF was carried out using immobilized nonlinear pH gradient 3–10 gel strips (24 cm Immobiline DryStrip gels, GE Healthcare) and Ettan II IPGphor system (GE Healthcare) for a total of 40–50 kVh.

    Techniques: Expressing

    Image of two-dimensional electrophoresis analysis in vehicle + middle cerebral artery occlusion (MCAO), retinoic acid (RA) + MCAO, vehicle + sham, and RA + sham animals ( n =5 per group). Isoelectric focusing was performed at pH 4–7 IPG strips and electrophoresed on 7.5–17.5% gradient SDS gels. Squares indicate the significantly changed protein spots between vehicle + MCAO and RA + MCAO animals.

    Journal: The Journal of Veterinary Medical Science

    Article Title: Identification of changed proteins by retinoic acid in cerebral ischemic damage: a proteomic study

    doi: 10.1292/jvms.22-0119

    Figure Lengend Snippet: Image of two-dimensional electrophoresis analysis in vehicle + middle cerebral artery occlusion (MCAO), retinoic acid (RA) + MCAO, vehicle + sham, and RA + sham animals ( n =5 per group). Isoelectric focusing was performed at pH 4–7 IPG strips and electrophoresed on 7.5–17.5% gradient SDS gels. Squares indicate the significantly changed protein spots between vehicle + MCAO and RA + MCAO animals.

    Article Snippet: Isoelectric focusing (IEF) was performed using immobilized pH gradient (IPG) gel strip (pH 4–7, 17 cm, Bio-Rad).

    Techniques: Electrophoresis