Review



gpr81  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Novus Biologicals gpr81
    Gpr81, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpr81/GPR81+Antibody+-+BSA+Free/pm41989058-138-86-87
    Average 93 stars, based on 19 article reviews
    gpr81 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Shoutai Wan regulates glycolysis imbalance at the maternal-fetal interface in threatened abortion mice.
    Article Snippet: .. The sections were labeled with the following primary antibodies for 12 h at 4 ◦C: HIF-1α (1:100, abcam#ab228649, Cambridge, MA, USA), LDHA antibody (1:100, abcam#ab52488), PKM2 antibody (1:100, proteintech#158221-AP, Wuhan, China), HK2 antibody (1:100, abcam#ab209847), MCT4 antibody (1:100, proteintech#22787-1-AP), and GPR81 (1:100, Novus#NLS2095, USA). ..

    Control:

    Article Title: GPR81 nuclear transportation is critical for cancer growth and progression in lung and other solid cancers
    Article Snippet: .. GPR81 or control IgG were immunoprecipitated from nuclear fractions using anti-GPR81 (NLS2095, Novus, United States) antibodies. ..

    Immunoprecipitation:

    Article Title: GPR81 nuclear transportation is critical for cancer growth and progression in lung and other solid cancers
    Article Snippet: .. GPR81 or control IgG were immunoprecipitated from nuclear fractions using anti-GPR81 (NLS2095, Novus, United States) antibodies. ..

    Incubation:

    Article Title: Shoutai Wan regulates glycolysis imbalance at the maternal-fetal interface in threatened abortion mice.
    Article Snippet: .. Primary antibodies against HIF-1α (1:500, abcam#ab228649), LDHA antibody (1:1000, abcam#ab52488), PKM2 antibody (1:1000, proteintech#15822-1-AP), HK2 antibody (1:1000, abcam#ab209847), MCT4 antibody (1:1000, proteintech#22787-1-AP), GPR81 (1:200, Novus#NLS2095), and β-actin (1:1000, proteintech#20536-1-AP) were incubated for 12 h at 4 ◦C. .. Then, we washed the membrane three times, with TBST for 5 min each, then treated it with a secondary antibody (goat anti-rabbit IgG, 1:5000, proteintech#PR30011) for 1 h at room temperature.

    other:

    Article Title: Activation of GPR81 by lactate drives tumour-induced cachexia.
    Article Snippet: For H89 treatment, the injection of H89 (10 mg per kg body weight in 100 μl PBS; MedChemExpress, HY-15979A) was started the day after implantation of the minipump and was given once every 2 days for 6 days, during which the body weight was recorded.



    Similar Products

    g protein coupled receptor 81 gpr81 antagonist 3 hydroxy butyrate  (MedChemExpress)
    94
    MedChemExpress g protein coupled receptor 81 gpr81 antagonist 3 hydroxy butyrate
    G Protein Coupled Receptor 81 Gpr81 Antagonist 3 Hydroxy Butyrate, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpr81/(R)-3-Hydroxybutanoic+acid/10__1158_slash_1078___0432__ccr___25___2352-65-37-46
    Average 94 stars, based on 1 article reviews
    g protein coupled receptor 81 gpr81 antagonist 3 hydroxy butyrate - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    gpr81  (Novus Biologicals)
    93
    Novus Biologicals gpr81
    Gpr81, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpr81/GPR81+Antibody+-+BSA+Free/pm41989058-138-86-87
    Average 93 stars, based on 1 article reviews
    gpr81 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    NLS2091  (novus biologicals)
    93
    novus biologicals NLS2091
    Nls2091, supplied by novus biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpr81/GPR81+Antibody+-+BSA+Free/pmc13085847-11-0-3
    Average 93 stars, based on 1 article reviews
    NLS2091 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    ap rrid ab 11182717 gpr81 antibody novus biologicals cat  (Novus Biologicals)
    93
    Novus Biologicals ap rrid ab 11182717 gpr81 antibody novus biologicals cat
    Ap Rrid Ab 11182717 Gpr81 Antibody Novus Biologicals Cat, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpr81/GPR81+Antibody+-+BSA+Free/pm41989058-200-113-118
    Average 93 stars, based on 1 article reviews
    ap rrid ab 11182717 gpr81 antibody novus biologicals cat - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    antibodies for gpr81  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc antibodies for gpr81
    G-protein-coupled receptor 81 <t>(GPR81)</t> may regulate monocarboxylate transporter 1 (MCT1) expression in hepatocytes in vitro . (A) Alpha mouse liver 12 (AML12) cells were treated with sodium L-lactate 20 and 40 mM for different time course. Western blot analysis was performed to assess the expression levels of GPR81, MCT1, and MCT4 in AML12 cells. (B) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 3 days. Western blot analysis was conducted to evaluate the expression levels of GPR81 and MCT1 in AML12 cells. The intensities of the bands in the Western blot images were quantified using Image Lab software and are displayed in the corresponding plot alongside the representative blot images. The protein levels were normalized to β-actin expression. Statistical significance compared with control is indicated by a P <0.05, b P <0.01, c P <0.001, lactate 20 mM treated group vs. control; d P <0.05, e P <0.01, f P <0.001, lactate 40 mM treated group vs. control; statistical significance compared with si-scramble is indicated by g P <0.05, h P <0.01, i P <0.001, with siGPR81 indicated j P <0.01.
    Antibodies For Gpr81, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpr81/pmc12996951-34-0-38
    Average 86 stars, based on 1 article reviews
    antibodies for gpr81 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    gpr81 antagonist 3 hydroxybutyric acid sodium  (MedChemExpress)
    94
    MedChemExpress gpr81 antagonist 3 hydroxybutyric acid sodium
    G-protein-coupled receptor 81 <t>(GPR81)</t> may regulate monocarboxylate transporter 1 (MCT1) expression in hepatocytes in vitro . (A) Alpha mouse liver 12 (AML12) cells were treated with sodium L-lactate 20 and 40 mM for different time course. Western blot analysis was performed to assess the expression levels of GPR81, MCT1, and MCT4 in AML12 cells. (B) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 3 days. Western blot analysis was conducted to evaluate the expression levels of GPR81 and MCT1 in AML12 cells. The intensities of the bands in the Western blot images were quantified using Image Lab software and are displayed in the corresponding plot alongside the representative blot images. The protein levels were normalized to β-actin expression. Statistical significance compared with control is indicated by a P <0.05, b P <0.01, c P <0.001, lactate 20 mM treated group vs. control; d P <0.05, e P <0.01, f P <0.001, lactate 40 mM treated group vs. control; statistical significance compared with si-scramble is indicated by g P <0.05, h P <0.01, i P <0.001, with siGPR81 indicated j P <0.01.
    Gpr81 Antagonist 3 Hydroxybutyric Acid Sodium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gpr81/3-Hydroxybutyric+acid/pm41540461-66-75-82
    Average 94 stars, based on 1 article reviews
    gpr81 antagonist 3 hydroxybutyric acid sodium - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    G-protein-coupled receptor 81 (GPR81) may regulate monocarboxylate transporter 1 (MCT1) expression in hepatocytes in vitro . (A) Alpha mouse liver 12 (AML12) cells were treated with sodium L-lactate 20 and 40 mM for different time course. Western blot analysis was performed to assess the expression levels of GPR81, MCT1, and MCT4 in AML12 cells. (B) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 3 days. Western blot analysis was conducted to evaluate the expression levels of GPR81 and MCT1 in AML12 cells. The intensities of the bands in the Western blot images were quantified using Image Lab software and are displayed in the corresponding plot alongside the representative blot images. The protein levels were normalized to β-actin expression. Statistical significance compared with control is indicated by a P <0.05, b P <0.01, c P <0.001, lactate 20 mM treated group vs. control; d P <0.05, e P <0.01, f P <0.001, lactate 40 mM treated group vs. control; statistical significance compared with si-scramble is indicated by g P <0.05, h P <0.01, i P <0.001, with siGPR81 indicated j P <0.01.

    Journal: Diabetes & Metabolism Journal

    Article Title: Lactate-Induced Lipid Accumulation in Hepatocytes through GPR81 Activation

    doi: 10.4093/dmj.2024.0531

    Figure Lengend Snippet: G-protein-coupled receptor 81 (GPR81) may regulate monocarboxylate transporter 1 (MCT1) expression in hepatocytes in vitro . (A) Alpha mouse liver 12 (AML12) cells were treated with sodium L-lactate 20 and 40 mM for different time course. Western blot analysis was performed to assess the expression levels of GPR81, MCT1, and MCT4 in AML12 cells. (B) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 3 days. Western blot analysis was conducted to evaluate the expression levels of GPR81 and MCT1 in AML12 cells. The intensities of the bands in the Western blot images were quantified using Image Lab software and are displayed in the corresponding plot alongside the representative blot images. The protein levels were normalized to β-actin expression. Statistical significance compared with control is indicated by a P <0.05, b P <0.01, c P <0.001, lactate 20 mM treated group vs. control; d P <0.05, e P <0.01, f P <0.001, lactate 40 mM treated group vs. control; statistical significance compared with si-scramble is indicated by g P <0.05, h P <0.01, i P <0.001, with siGPR81 indicated j P <0.01.

    Article Snippet: Antibodies for GPR81 (MBS821939, My BioSource, San Diego, CA, USA), MCT1 (sc-365501, Santa Cruz Biotechnology, Santa Cruz, CA, USA), MCT4 (sc-376140, Santa Cruz Biotechnology), β-actin (sc-47778, Santa Cruz Biotechnology), phosphorylation 5’ adenosine monophosphate-activated protein kinase (p-AMPK) (Thr172 #2535, Cell Signaling Technology, Danvers, MA, USA), AMPK (#2532, Cell Signaling Technology), peroxisome proliferator-activated receptor alpha (PPARα) (sc-398394 Santa Cruz Biotechnology), sterol regulatory element-binding protein 1 (SREBP1) (NB100-2215, Novus Biologicals, Centennial, CO, USA), stearoyl-CoA desaturase-1 (SCD1) (#2794, Cell Signaling Technology), acetyl-CoA carboxylase (ACC) (#3676, Cell Signaling Technology), and α-tubulin (#12351, Cell Signaling Technology) were used in this study.

    Techniques: Expressing, In Vitro, Western Blot, Small Interfering RNA, Software, Control

    G-protein-coupled receptor 81 (GPR81) played a major role in regulating lipid accumulation in lactate-treated alpha mouse liver 12 (AML12) cells. (A) AML12 cells were treated with L-lactate at 20 mM with or without AZD3965 at 100 nM for 4 days. Lipid accumulation was evaluated using Oil Red O staining. (B) AML12 cells were treated with sodium L-lactate 20 and 40 mM for 3 days. The isolation of plasma membrane (PM) and cytosol fractions was performed, and the expression of GPR81 and monocarboxylate transporter 1 (MCT1) in AML12 cells was assessed. Na/K ATPase served as a housekeeping marker for the PM, while tubulin served as a housekeeping marker for the cytosol. (C) Immunofluorescence of MCT1 (red) and nuclei (4ʹ,6-diamidino2-phenylindole [DAPI] blue). Scale bar: 20 μm. (D) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 4 days. The accumulation of lipids in AML12 cells was visualized using Oil Red O staining. Statistical significance compared with control is indicated by a P <0.05, b P <0.01. Statistical significance compared with si-scramble is indicated by c P <0.05, d P <0.001, with siGPR81 indicated e P <0.001.

    Journal: Diabetes & Metabolism Journal

    Article Title: Lactate-Induced Lipid Accumulation in Hepatocytes through GPR81 Activation

    doi: 10.4093/dmj.2024.0531

    Figure Lengend Snippet: G-protein-coupled receptor 81 (GPR81) played a major role in regulating lipid accumulation in lactate-treated alpha mouse liver 12 (AML12) cells. (A) AML12 cells were treated with L-lactate at 20 mM with or without AZD3965 at 100 nM for 4 days. Lipid accumulation was evaluated using Oil Red O staining. (B) AML12 cells were treated with sodium L-lactate 20 and 40 mM for 3 days. The isolation of plasma membrane (PM) and cytosol fractions was performed, and the expression of GPR81 and monocarboxylate transporter 1 (MCT1) in AML12 cells was assessed. Na/K ATPase served as a housekeeping marker for the PM, while tubulin served as a housekeeping marker for the cytosol. (C) Immunofluorescence of MCT1 (red) and nuclei (4ʹ,6-diamidino2-phenylindole [DAPI] blue). Scale bar: 20 μm. (D) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 4 days. The accumulation of lipids in AML12 cells was visualized using Oil Red O staining. Statistical significance compared with control is indicated by a P <0.05, b P <0.01. Statistical significance compared with si-scramble is indicated by c P <0.05, d P <0.001, with siGPR81 indicated e P <0.001.

    Article Snippet: Antibodies for GPR81 (MBS821939, My BioSource, San Diego, CA, USA), MCT1 (sc-365501, Santa Cruz Biotechnology, Santa Cruz, CA, USA), MCT4 (sc-376140, Santa Cruz Biotechnology), β-actin (sc-47778, Santa Cruz Biotechnology), phosphorylation 5’ adenosine monophosphate-activated protein kinase (p-AMPK) (Thr172 #2535, Cell Signaling Technology, Danvers, MA, USA), AMPK (#2532, Cell Signaling Technology), peroxisome proliferator-activated receptor alpha (PPARα) (sc-398394 Santa Cruz Biotechnology), sterol regulatory element-binding protein 1 (SREBP1) (NB100-2215, Novus Biologicals, Centennial, CO, USA), stearoyl-CoA desaturase-1 (SCD1) (#2794, Cell Signaling Technology), acetyl-CoA carboxylase (ACC) (#3676, Cell Signaling Technology), and α-tubulin (#12351, Cell Signaling Technology) were used in this study.

    Techniques: Staining, Isolation, Clinical Proteomics, Membrane, Expressing, Marker, Immunofluorescence, Small Interfering RNA, Control

    Lactate-induced G-protein-coupled receptor 81 (GPR81) activation promotes hepatocyte lipogenesis and fatty acid storage in vitro . (A) Alpha mouse liver 12 (AML12) cells were treated with sodium L-lactate 20 and 40 mM for 3 days. (B) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 3 days. Cell lysates were then analyzed via Western blot to determine protein levels. Representative images of immunoblots of lipogenesis markers. β-Actin is a loading control. SREBP1c, sterol regulatory element-binding protein 1c; ACC, acetyl-CoA carboxylase; SCD1, stearoyl-CoA desaturase-1; FABP4, fatty acid binding protein 4; PPARα, peroxisome proliferator-activated receptor alpha; CPT1, carnitine palmitoyltransferase I; NS, not significant. Statistical significance compared with control is indicated by a P <0.05, b P <0.01, c P <0.0001. Statistical significance compared with si-scramble is indicated by d P <0.05, e P <0.01, with siGPR81 indicated f P <0.05, g P <0.01.

    Journal: Diabetes & Metabolism Journal

    Article Title: Lactate-Induced Lipid Accumulation in Hepatocytes through GPR81 Activation

    doi: 10.4093/dmj.2024.0531

    Figure Lengend Snippet: Lactate-induced G-protein-coupled receptor 81 (GPR81) activation promotes hepatocyte lipogenesis and fatty acid storage in vitro . (A) Alpha mouse liver 12 (AML12) cells were treated with sodium L-lactate 20 and 40 mM for 3 days. (B) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 3 days. Cell lysates were then analyzed via Western blot to determine protein levels. Representative images of immunoblots of lipogenesis markers. β-Actin is a loading control. SREBP1c, sterol regulatory element-binding protein 1c; ACC, acetyl-CoA carboxylase; SCD1, stearoyl-CoA desaturase-1; FABP4, fatty acid binding protein 4; PPARα, peroxisome proliferator-activated receptor alpha; CPT1, carnitine palmitoyltransferase I; NS, not significant. Statistical significance compared with control is indicated by a P <0.05, b P <0.01, c P <0.0001. Statistical significance compared with si-scramble is indicated by d P <0.05, e P <0.01, with siGPR81 indicated f P <0.05, g P <0.01.

    Article Snippet: Antibodies for GPR81 (MBS821939, My BioSource, San Diego, CA, USA), MCT1 (sc-365501, Santa Cruz Biotechnology, Santa Cruz, CA, USA), MCT4 (sc-376140, Santa Cruz Biotechnology), β-actin (sc-47778, Santa Cruz Biotechnology), phosphorylation 5’ adenosine monophosphate-activated protein kinase (p-AMPK) (Thr172 #2535, Cell Signaling Technology, Danvers, MA, USA), AMPK (#2532, Cell Signaling Technology), peroxisome proliferator-activated receptor alpha (PPARα) (sc-398394 Santa Cruz Biotechnology), sterol regulatory element-binding protein 1 (SREBP1) (NB100-2215, Novus Biologicals, Centennial, CO, USA), stearoyl-CoA desaturase-1 (SCD1) (#2794, Cell Signaling Technology), acetyl-CoA carboxylase (ACC) (#3676, Cell Signaling Technology), and α-tubulin (#12351, Cell Signaling Technology) were used in this study.

    Techniques: Activation Assay, In Vitro, Small Interfering RNA, Western Blot, Control, Binding Assay

    Lactate-mediated G-protein-coupled receptor 81 (GPR81) activation regulates 5’ adenosine monophosphate-activated protein kinase (AMPK) in alpha mouse liver 12 (AML12) cells. (A) AML12 cells were treated with sodium L-lactate 20 and 40 mM for 3 days. (B) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 4 days. Cell lysates were analyzed by Western blot to measure the protein levels of phosphorylation AMPK (p-AMPK) and AMPK. (C) AML12 cells were treated with lactate for 2 days and then co-treated with or without 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) 100 µM for next 2 days. Lipid accumulation was evaluated using Oil Red O staining. (D) AML12 cells were treated with lactate for 2 days and then co-treated with or without AICAR 100 µM for next 1 day. Representative images of immunoblots of mature form of sterol regulatory element-binding protein 1c (SREBP1c), CD36, and fatty acid binding protein 4 (FABP4). β-Actin or tubulin is a loading control. NS, not significant. Statistical significance compared with control is indicated by a P <0.05, b P <0.01, c P <0.0001. Statistical significance compared with si-scramble is indicated by d P <0.01, with siGPR81 indicated e P <0.01. Statistical significance compared with lactate 20 mM is indicated by f P <0.05, g P <0.01, h P <0.001.

    Journal: Diabetes & Metabolism Journal

    Article Title: Lactate-Induced Lipid Accumulation in Hepatocytes through GPR81 Activation

    doi: 10.4093/dmj.2024.0531

    Figure Lengend Snippet: Lactate-mediated G-protein-coupled receptor 81 (GPR81) activation regulates 5’ adenosine monophosphate-activated protein kinase (AMPK) in alpha mouse liver 12 (AML12) cells. (A) AML12 cells were treated with sodium L-lactate 20 and 40 mM for 3 days. (B) AML12 cells were treated with small interfering RNA (siRNA) 50 nM with or without lactate 20 mM for 4 days. Cell lysates were analyzed by Western blot to measure the protein levels of phosphorylation AMPK (p-AMPK) and AMPK. (C) AML12 cells were treated with lactate for 2 days and then co-treated with or without 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) 100 µM for next 2 days. Lipid accumulation was evaluated using Oil Red O staining. (D) AML12 cells were treated with lactate for 2 days and then co-treated with or without AICAR 100 µM for next 1 day. Representative images of immunoblots of mature form of sterol regulatory element-binding protein 1c (SREBP1c), CD36, and fatty acid binding protein 4 (FABP4). β-Actin or tubulin is a loading control. NS, not significant. Statistical significance compared with control is indicated by a P <0.05, b P <0.01, c P <0.0001. Statistical significance compared with si-scramble is indicated by d P <0.01, with siGPR81 indicated e P <0.01. Statistical significance compared with lactate 20 mM is indicated by f P <0.05, g P <0.01, h P <0.001.

    Article Snippet: Antibodies for GPR81 (MBS821939, My BioSource, San Diego, CA, USA), MCT1 (sc-365501, Santa Cruz Biotechnology, Santa Cruz, CA, USA), MCT4 (sc-376140, Santa Cruz Biotechnology), β-actin (sc-47778, Santa Cruz Biotechnology), phosphorylation 5’ adenosine monophosphate-activated protein kinase (p-AMPK) (Thr172 #2535, Cell Signaling Technology, Danvers, MA, USA), AMPK (#2532, Cell Signaling Technology), peroxisome proliferator-activated receptor alpha (PPARα) (sc-398394 Santa Cruz Biotechnology), sterol regulatory element-binding protein 1 (SREBP1) (NB100-2215, Novus Biologicals, Centennial, CO, USA), stearoyl-CoA desaturase-1 (SCD1) (#2794, Cell Signaling Technology), acetyl-CoA carboxylase (ACC) (#3676, Cell Signaling Technology), and α-tubulin (#12351, Cell Signaling Technology) were used in this study.

    Techniques: Activation Assay, Small Interfering RNA, Western Blot, Phospho-proteomics, Staining, Binding Assay, Control