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camp glosensor (20f) biosensor hek293g  (Promega)

 
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    Structured Review

    Promega camp glosensor (20f) biosensor hek293g
    Effect of PSB603‐BY630 on <t>Glosensor</t> cAMP concentration‐response curves to the A 2B ‐selective agonist BAY 60–6583 in (A) <t>HEK293G</t> cells endogenously expressing A 2B R or (B) HEK293G cells overexpressing NLuc‐A 2B R. Concentration response curves were obtained in the absence and presence of 20 nM or 200 nM PSB603‐BY630. Values are mean ± S.E.M. of five separate experiments carried out in triplicate. Data represent peak luminescence response and are expressed as a percentage of the peak luminescence response to 3 μM BAY 60–6583 (in a) or 0.3 μM BAY 60–6583 (in b) obtained in the absence of antagonist in each individual experiment.
    Camp Glosensor (20f) Biosensor Hek293g, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glosensor-20f+camp+biosensor/hek293+cells/pmc11191602-68-9-18
    Average 90 stars, based on 1 article reviews
    camp glosensor (20f) biosensor hek293g - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "A novel and selective fluorescent ligand for the study of adenosine A 2B receptors"

    Article Title: A novel and selective fluorescent ligand for the study of adenosine A 2B receptors

    Journal: Pharmacology Research & Perspectives

    doi: 10.1002/prp2.1223

    Effect of PSB603‐BY630 on Glosensor cAMP concentration‐response curves to the A 2B ‐selective agonist BAY 60–6583 in (A) HEK293G cells endogenously expressing A 2B R or (B) HEK293G cells overexpressing NLuc‐A 2B R. Concentration response curves were obtained in the absence and presence of 20 nM or 200 nM PSB603‐BY630. Values are mean ± S.E.M. of five separate experiments carried out in triplicate. Data represent peak luminescence response and are expressed as a percentage of the peak luminescence response to 3 μM BAY 60–6583 (in a) or 0.3 μM BAY 60–6583 (in b) obtained in the absence of antagonist in each individual experiment.
    Figure Legend Snippet: Effect of PSB603‐BY630 on Glosensor cAMP concentration‐response curves to the A 2B ‐selective agonist BAY 60–6583 in (A) HEK293G cells endogenously expressing A 2B R or (B) HEK293G cells overexpressing NLuc‐A 2B R. Concentration response curves were obtained in the absence and presence of 20 nM or 200 nM PSB603‐BY630. Values are mean ± S.E.M. of five separate experiments carried out in triplicate. Data represent peak luminescence response and are expressed as a percentage of the peak luminescence response to 3 μM BAY 60–6583 (in a) or 0.3 μM BAY 60–6583 (in b) obtained in the absence of antagonist in each individual experiment.

    Techniques Used: Concentration Assay, Expressing

    Related Articles

    Recombinant:

    Article Title: S-nitrosylation is required for β 2 AR desensitization and experimental asthma.
    Article Snippet: In brief Post-translational modification of the b2AR by S-nitrosylation, resulting from receptor activation or direct modification, promotes receptor desensitization and caveolar internalization.. Preventing b2AR S-nitrosylation leads to sustained receptor signaling, thereby increasing cAMP, Erk, and NO. Mice with mutant b2AR refractory to S-nitrosylation are resistant to bronchoconstriction, inflammation, and development of asthma, suggesting that this newly identified desensitization mechanism may be targeted therapeutically in obstructive airway diseases.

    Mutagenesis:

    Article Title: S-nitrosylation is required for β 2 AR desensitization and experimental asthma.
    Article Snippet: In brief Post-translational modification of the b2AR by S-nitrosylation, resulting from receptor activation or direct modification, promotes receptor desensitization and caveolar internalization.. Preventing b2AR S-nitrosylation leads to sustained receptor signaling, thereby increasing cAMP, Erk, and NO. Mice with mutant b2AR refractory to S-nitrosylation are resistant to bronchoconstriction, inflammation, and development of asthma, suggesting that this newly identified desensitization mechanism may be targeted therapeutically in obstructive airway diseases.

    Software:

    Article Title: S-nitrosylation is required for β 2 AR desensitization and experimental asthma.
    Article Snippet: In brief Post-translational modification of the b2AR by S-nitrosylation, resulting from receptor activation or direct modification, promotes receptor desensitization and caveolar internalization.. Preventing b2AR S-nitrosylation leads to sustained receptor signaling, thereby increasing cAMP, Erk, and NO. Mice with mutant b2AR refractory to S-nitrosylation are resistant to bronchoconstriction, inflammation, and development of asthma, suggesting that this newly identified desensitization mechanism may be targeted therapeutically in obstructive airway diseases.



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    Effect of PSB603‐BY630 on <t>Glosensor</t> cAMP concentration‐response curves to the A 2B ‐selective agonist BAY 60–6583 in (A) <t>HEK293G</t> cells endogenously expressing A 2B R or (B) HEK293G cells overexpressing NLuc‐A 2B R. Concentration response curves were obtained in the absence and presence of 20 nM or 200 nM PSB603‐BY630. Values are mean ± S.E.M. of five separate experiments carried out in triplicate. Data represent peak luminescence response and are expressed as a percentage of the peak luminescence response to 3 μM BAY 60–6583 (in a) or 0.3 μM BAY 60–6583 (in b) obtained in the absence of antagonist in each individual experiment.
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    Effect of PSB603‐BY630 on <t>Glosensor</t> cAMP concentration‐response curves to the A 2B ‐selective agonist BAY 60–6583 in (A) <t>HEK293G</t> cells endogenously expressing A 2B R or (B) HEK293G cells overexpressing NLuc‐A 2B R. Concentration response curves were obtained in the absence and presence of 20 nM or 200 nM PSB603‐BY630. Values are mean ± S.E.M. of five separate experiments carried out in triplicate. Data represent peak luminescence response and are expressed as a percentage of the peak luminescence response to 3 μM BAY 60–6583 (in a) or 0.3 μM BAY 60–6583 (in b) obtained in the absence of antagonist in each individual experiment.
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    Image Search Results


    Effect of PSB603‐BY630 on Glosensor cAMP concentration‐response curves to the A 2B ‐selective agonist BAY 60–6583 in (A) HEK293G cells endogenously expressing A 2B R or (B) HEK293G cells overexpressing NLuc‐A 2B R. Concentration response curves were obtained in the absence and presence of 20 nM or 200 nM PSB603‐BY630. Values are mean ± S.E.M. of five separate experiments carried out in triplicate. Data represent peak luminescence response and are expressed as a percentage of the peak luminescence response to 3 μM BAY 60–6583 (in a) or 0.3 μM BAY 60–6583 (in b) obtained in the absence of antagonist in each individual experiment.

    Journal: Pharmacology Research & Perspectives

    Article Title: A novel and selective fluorescent ligand for the study of adenosine A 2B receptors

    doi: 10.1002/prp2.1223

    Figure Lengend Snippet: Effect of PSB603‐BY630 on Glosensor cAMP concentration‐response curves to the A 2B ‐selective agonist BAY 60–6583 in (A) HEK293G cells endogenously expressing A 2B R or (B) HEK293G cells overexpressing NLuc‐A 2B R. Concentration response curves were obtained in the absence and presence of 20 nM or 200 nM PSB603‐BY630. Values are mean ± S.E.M. of five separate experiments carried out in triplicate. Data represent peak luminescence response and are expressed as a percentage of the peak luminescence response to 3 μM BAY 60–6583 (in a) or 0.3 μM BAY 60–6583 (in b) obtained in the absence of antagonist in each individual experiment.

    Article Snippet: A clonal HEK 293 cell line stably expressing the cAMP GloSensor (20F) biosensor (HEK293G) , was obtained from Promega Corporation (Madison, WI, USA).

    Techniques: Concentration Assay, Expressing

    Glosensor cyclic AMP responses to CGS 21680 and BAY 60‐6583 in HEK293G cells. (A) Concentration‐response curves for agonist‐stimulated cAMP Glosensor luminescence responses in HEK293G cells in response to CGS 21680 and BAY 60‐6583. (B) The effect of simultaneous addition of 100 µM BAY 60‐6583 on the concentration‐response curve to CGS 21680, following 2 h pretreatment with PSB 603 (0.1 µM). Also shown is the effect of simultaneous addition of 10 µM SCH 58261 on the responses to CGS 21680 in the presence of PSB 603 (0.1 µM). (C, D) The effect of 100 µM BAY 60‐6583 on the response to 10 µM formoterol in the (C) absence and (D) presence of 0.1 µM PSB 603. (E) The effect of simultaneous addition of 10 µM SCH 58261 on the response to 10 µM formoterol. Data represent mean ± SEM of the peak luminescence response of 5 separate experiments, each carried out in triplicate. Data are expressed as a percentage of the peak luminescence response obtained with 100 µM NECA measured in the same experiment. ** p < .05 (unpaired t ‐test)

    Journal: The FASEB Journal

    Article Title: Regionally selective cardiovascular responses to adenosine A 2A and A 2B receptor activation

    doi: 10.1096/fj.202101945R

    Figure Lengend Snippet: Glosensor cyclic AMP responses to CGS 21680 and BAY 60‐6583 in HEK293G cells. (A) Concentration‐response curves for agonist‐stimulated cAMP Glosensor luminescence responses in HEK293G cells in response to CGS 21680 and BAY 60‐6583. (B) The effect of simultaneous addition of 100 µM BAY 60‐6583 on the concentration‐response curve to CGS 21680, following 2 h pretreatment with PSB 603 (0.1 µM). Also shown is the effect of simultaneous addition of 10 µM SCH 58261 on the responses to CGS 21680 in the presence of PSB 603 (0.1 µM). (C, D) The effect of 100 µM BAY 60‐6583 on the response to 10 µM formoterol in the (C) absence and (D) presence of 0.1 µM PSB 603. (E) The effect of simultaneous addition of 10 µM SCH 58261 on the response to 10 µM formoterol. Data represent mean ± SEM of the peak luminescence response of 5 separate experiments, each carried out in triplicate. Data are expressed as a percentage of the peak luminescence response obtained with 100 µM NECA measured in the same experiment. ** p < .05 (unpaired t ‐test)

    Article Snippet: A clonal HEK 293 cell line stably expressing the cAMP GloSensor (20F) biosensor (HEK293G) , was obtained from Promega (Madison, WI, USA).

    Techniques: Concentration Assay

    Journal: Cell

    Article Title: Human Gain-of-Function MC4R Variants Show Signaling Bias and Protect against Obesity

    doi: 10.1016/j.cell.2019.03.044

    Figure Lengend Snippet:

    Article Snippet: GloSensor cAMP biosensor - pGloSensor 20F plasmid , Promega , Cat#E1171.

    Techniques: Virus, Recombinant, Plasmid Preparation, Software