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Promega glosensor camp reporter plasmid p22f
Glosensor Camp Reporter Plasmid P22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+camp+plasmid/glosensor+camp+reporter+plasmid+p22f/pm40368898-308-5-10
Average 90 stars, based on 1 article reviews
glosensor camp reporter plasmid p22f - by Bioz Stars, 2026-09
90/100 stars

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Plasmid Preparation:

Article Title: Long Receptor Residence Time of C26 Contributes to Super Agonist Activity at the Human β2 Adrenoceptor.
Article Snippet: ALPHAScreen cAMP detection kit, [3H]-DHA (dihydroalprenolol), and 384- and 96-well ViewPlates were purchased from PerkinElmer Life Sciences (Waltham, MA). .. GloSensor cAMP reagent and GloSensor cAMP plasmid were purchased from Promega (Hampshire, UK). .. Bovine serum albumin (BSA), Tween-20, adrenaline (epinephrine), isoprenaline, dimethylsulfoxide (DMSO), GTP, and ascorbic acid were purchased from Sigma-Aldrich (Poole, UK).

Article Title: Discovery of Human Signaling Systems: Pairing Peptides to G Protein-Coupled Receptors
Article Snippet: Roth Lab PRESTO-Tango GPCR Kit , , Addgene Kit # 1000000068. .. GloSensor cAMP plasmid , Promega , Cat # E2301. ..

Article Title: Usefulness for the combination of G protein- and β-arrestin-biased ligands of μ-opioid receptors: Prevention of antinociceptive tolerance
Article Snippet: Briefly, HEK293-MOR-FLAG cells that stably expressed μ-opioid receptors were plated at 1.5 × 10 4 cells/μl in 96-well plates in high-glucose Dulbecco’s modified Eagle medium containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 700 μg/ml G418 disulfate aqueous solution (Nacalai Tesque, Kyoto, Japan). .. Next day, cells were transiently transfected with GloSensor cAMP Plasmid (Promega) using the FuGENE HD Transfection Reagent (Promega). .. Twenty-four hours after transfection, medium is changed to equilibration medium (88% CO 2 -independent medium, 10% FBS, 2% GloSensor cAMP Reagent stock solution), and then cells were incubated for 2 h. After 5-min application with forskolin (130 μM), drugs were treated to the well for 30 min. Chemiluminescence in each step was measured by a microplate reader (GLOMAX, Promega).

Article Title: Functional histamine H 3 and adenosine A 2A receptor heteromers in recombinant cells and rat striatum
Article Snippet: .. Briefly, HEK-293T cells transfected with 3xHA-A 2A R, 3xHA-H 3 R or a mixture of both plasmids (1 μg each) together with Glosensor cAMP plasmid (Promega, Madison, WI, USA) were seeded in a white 384-well low-volume plate (25,000 cells/well in 7.5 μl medium) and incubated with Glo-equilibrium medium (7.5 μl, Promega). .. Drugs under test were added in a 5 μl volume (4x in HBSS solution) and endogenous cAMP luminescence was measured in real-time in a Flexstation 3 apparatus (Molecular Devices, Sunnyvale, CA, USA).

Article Title: Inhibition of the Proliferation of Human Lung Fibroblasts by Prostacyclin Receptor Agonists is Linked to a Sustained cAMP Signal in the Nucleus
Article Snippet: .. Human lung fibroblasts were electroporated with the GloSensor cAMP plasmid (Promega) using the 4D-Nucleofector System and P2 primary cell 4D-Nucelofector X Kit S (Lonza). ..

Transfection:

Article Title: Usefulness for the combination of G protein- and β-arrestin-biased ligands of μ-opioid receptors: Prevention of antinociceptive tolerance
Article Snippet: Briefly, HEK293-MOR-FLAG cells that stably expressed μ-opioid receptors were plated at 1.5 × 10 4 cells/μl in 96-well plates in high-glucose Dulbecco’s modified Eagle medium containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 700 μg/ml G418 disulfate aqueous solution (Nacalai Tesque, Kyoto, Japan). .. Next day, cells were transiently transfected with GloSensor cAMP Plasmid (Promega) using the FuGENE HD Transfection Reagent (Promega). .. Twenty-four hours after transfection, medium is changed to equilibration medium (88% CO 2 -independent medium, 10% FBS, 2% GloSensor cAMP Reagent stock solution), and then cells were incubated for 2 h. After 5-min application with forskolin (130 μM), drugs were treated to the well for 30 min. Chemiluminescence in each step was measured by a microplate reader (GLOMAX, Promega).

Article Title: Functional histamine H 3 and adenosine A 2A receptor heteromers in recombinant cells and rat striatum
Article Snippet: .. Briefly, HEK-293T cells transfected with 3xHA-A 2A R, 3xHA-H 3 R or a mixture of both plasmids (1 μg each) together with Glosensor cAMP plasmid (Promega, Madison, WI, USA) were seeded in a white 384-well low-volume plate (25,000 cells/well in 7.5 μl medium) and incubated with Glo-equilibrium medium (7.5 μl, Promega). .. Drugs under test were added in a 5 μl volume (4x in HBSS solution) and endogenous cAMP luminescence was measured in real-time in a Flexstation 3 apparatus (Molecular Devices, Sunnyvale, CA, USA).

Incubation:

Article Title: Functional histamine H 3 and adenosine A 2A receptor heteromers in recombinant cells and rat striatum
Article Snippet: .. Briefly, HEK-293T cells transfected with 3xHA-A 2A R, 3xHA-H 3 R or a mixture of both plasmids (1 μg each) together with Glosensor cAMP plasmid (Promega, Madison, WI, USA) were seeded in a white 384-well low-volume plate (25,000 cells/well in 7.5 μl medium) and incubated with Glo-equilibrium medium (7.5 μl, Promega). .. Drugs under test were added in a 5 μl volume (4x in HBSS solution) and endogenous cAMP luminescence was measured in real-time in a Flexstation 3 apparatus (Molecular Devices, Sunnyvale, CA, USA).

other:

Article Title: Adenosine receptor modulators for the treatment of circadian rhythm disorders
Article Snippet: Unless stated otherwise, forskolin was used as a positive control, and 1% DMSO was used as a negative control. cAMP GloSensor Assay The GloSensorTM cAMP Assay (Promega) was used for detecting changes in the intracellular levels of cAMP.



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KEY RESOURCES TABLE

Journal: Neuron

Article Title: In vivo photopharmacology with light-activated opioid drugs

doi: 10.1016/j.neuron.2023.09.017

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Media in 10 cm TC dishes with HEK 293T cells (at around 70% confluence) were replaced with Opti-MEM (Invitrogen) followed by addition of a mixture of the GPCR plasmid, the GloSensor (Promega) 22F cAMP dependent reporter plasmid (Promega), and Lipofectamine 2000 (Invitrogen) in Opti-MEM.

Techniques: Virus, Recombinant, cAMP Assay, Software