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camp sensor glosensor 22f  (Promega)

 
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    Promega camp sensor glosensor 22f
    Camp Sensor Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glosensor+22f+camp+sensor/pglosensor+22f+camp+plasmid/pmc08554411-30-3-5
    Average 90 stars, based on 1 article reviews
    camp sensor glosensor 22f - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
    Article Snippet: DNA reagent , Sh.5HTR , Data repository , GenBank accession number MG813903 , . .. Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , . .. Commercial assay or kit , D-luciferin sodium salt , Company , Gold Biotechnology; LUCK-100

    Clone Assay:

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
    Article Snippet: DNA reagent , Sh.5HTR , Data repository , GenBank accession number MG813903 , . .. Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , . .. Commercial assay or kit , D-luciferin sodium salt , Company , Gold Biotechnology; LUCK-100

    Bioassay:

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
    Article Snippet: DNA reagent , Sh.5HTR , Data repository , GenBank accession number MG813903 , . .. Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , . .. Commercial assay or kit , D-luciferin sodium salt , Company , Gold Biotechnology; LUCK-100

    Generated:

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
    Article Snippet: DNA reagent , Sh.5HTR , Data repository , GenBank accession number MG813903 , . .. Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , . .. Commercial assay or kit , D-luciferin sodium salt , Company , Gold Biotechnology; LUCK-100

    Luciferase:

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
    Article Snippet: DNA reagent , Sh.5HTR , Data repository , GenBank accession number MG813903 , . .. Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , . .. Commercial assay or kit , D-luciferin sodium salt , Company , Gold Biotechnology; LUCK-100

    Transfection:

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
    Article Snippet: DNA reagent , Sh.5HTR , Data repository , GenBank accession number MG813903 , . .. Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , . .. Commercial assay or kit , D-luciferin sodium salt , Company , Gold Biotechnology; LUCK-100

    Recombinant:

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
    Article Snippet: DNA reagent , Sh.5HTR , Data repository , GenBank accession number MG813903 , . .. Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , . .. Commercial assay or kit , D-luciferin sodium salt , Company , Gold Biotechnology; LUCK-100

    Software:

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy
    Article Snippet: DNA reagent , Sh.5HTR , Data repository , GenBank accession number MG813903 , . .. Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , . .. Commercial assay or kit , D-luciferin sodium salt , Company , Gold Biotechnology; LUCK-100



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    https://www.bioz.com/product/glosensor+22f+camp+sensor/pglosensor+22f+camp+plasmid/pmc08554411-30-3-5
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    ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered <t>GloSensor</t> luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.
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    ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered <t>GloSensor</t> luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.
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    Image Search Results


    ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered GloSensor luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.

    Journal: eLife

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy

    doi: 10.7554/eLife.35755

    Figure Lengend Snippet: ( A ) Confocal images showing cell surface expression in mammalian HEK293 cells of 5-HT receptors cloned from S. mansoni ( Sm .5HTR L ), S. haematobium ( Sh. 5HTR) and S. japonicum ( Sj .5HTR) localized by COOH-terminally tagged eGFP. Scalebar, 50 µm. ( B ) Schematic of luminescent cAMP sensor bioassay. cAMP generated by schistosome 5-HTRs (blue) binds the engineered GloSensor luciferase, switching the sensor to a more active conformation resulting in enhanced luminescence output. ( C ) Kinetics of signal following the addition of 5-HT (1 µM) to HEK293 cells co-transfected with luminescent cAMP sensor and individual schistosome 5-HT receptors. Open circles, cells not transfected with 5-HT receptor, colored circles represent measurements in cells transfected with Sm .5HTR L (black), ( Sh. 5HTR (blue) and Sj .5HTR (red). ( D ) Serotonin dose-response curves for each of the three receptors (colored circles) and HEK293 cells expressing the cAMP sensor alone (open circles). Data reflect mean ± standard error of at least 3 biological replicates.

    Article Snippet: Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , .

    Techniques: Expressing, Clone Assay, Bioassay, Generated, Luciferase, Transfection

    Journal: eLife

    Article Title: Coalescing beneficial host and deleterious antiparasitic actions as an antischistosomal strategy

    doi: 10.7554/eLife.35755

    Figure Lengend Snippet:

    Article Snippet: Commercial assay or kit , GloSensor 22F cAMP sensor , Company , Promega; E2301 , .

    Techniques: Recombinant, Software