expression rrid addgene 232062 egfp rab8a rrid addgene 49543 gfp rab7 rrid addgene 12605 pqe30 darpin e11 n (Addgene inc)

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Addgene inc expression rrid addgene 232062 egfp rab8a rrid addgene 49543 gfp rab7 rrid addgene 12605 pqe30 darpin e11 n
Expression Rrid Addgene 232062 Egfp Rab8a Rrid Addgene 49543 Gfp Rab7 Rrid Addgene 12605 Pqe30 Darpin E11 N, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rab8a/EGFP-Rab8+(Plasmid+%2349543)/pmc12136037__sciadv%2Eadt2050_sm-44-84-86
Average 92 stars, based on 7 article reviews

expression rrid addgene 232062 egfp rab8a rrid addgene 49543 gfp rab7 rrid addgene 12605 pqe30 darpin e11 n - by Bioz Stars, 2026-11

92/100 stars

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Transfection:

Article Title: Type-II kinase inhibitors that target Parkinson’s Disease-associated LRRK2
Article Snippet: To determine the IC50, a non-linear regression with variable slope was fitted to the datapoints with GraphPad Prism. (For step-by-step protocol see: dx.doi.org/10.17504/protocols.io.6qpvr385ovmk/v2 ). .. 293T cells (American Type Culture Collection, ATCC cat. no. CRL-3216, RRID: CVCL_0063) were transfected with 1000 ng of wild-type full-length LRRK2 (pcDNA5-LRRK2) and 500 ng GFP-Rab8A (Addgene, RRID: Addgene_49543) using PEI (Polyethylenimine, Polysciences) (Supporting Information Table S4 ). .. After 48 h, transfected cells were treated with either DMSO, 500 nM MLi-2 (Tocris), or various concentrations of RN277 or RN341 for 4 h at 37 °C and 5 % CO2.

Article Title: Type II kinase inhibitors that target Parkinson’s disease–associated LRRK2
Article Snippet: To determine the IC 50 , a nonlinear regression with variable slope was fitted to the datapoints with GraphPad Prism ( www.graphpad.com/ , RRID:SCR_002798). (For step-by-step protocol, see dx.doi.org/10.17504/protocols.io.6qpvr385ovmk/v2 .) .. For Western blot analysis of LRRK2 kinase activity in cells, 293T cells (ATCC, CRL-3216; RRID: CVCL_0063) were transfected with 1000 ng of wild-type full-length LRRK2 (pcDNA5-LRRK2, RRID: Addgene_229019) and 500 ng of GFP-Rab8A (RRID: Addgene_49543) using polyethylenimine (Polysciences). .. For analysis of inhibitor activity against wild-type and G2019S mutant LRRK2, 293T cells were transfected with 500 ng of GFP-Rab8a and either 1000 ng of GFP-11 tagged wild-type LRRK2 (pcDNA5-FRT-TO-GFP11-LRRK2, RRID: Addgene_231174) or GFP-11 tagged G2019S LRRK2 (pcDNA5-FRT-TO-GFP11-LRRK2-G2019S, RRID: Addgene_231175).

Article Title: A designed ankyrin-repeat protein that targets Parkinson’s disease-associated LRRK2
Article Snippet: .. 293T cells (American Type Culture Collection, ATCC cat. no. CRL-3216, RRID: CVCL_0063) were transfected with 1000 ng of WT, full-length, untagged LRRK2 (pCDNA5-LRRK2) and 500 ng GFP-Rab8A (Addgene; RRID: Addgene_49543) or 1000 ng LRRK2, 500 ng GFP-Rab8A, and 500 ng of 8xHis-DARPin E11-3xFLAG using polyethylenimine. .. After 48 h, cells transfected only with LRRK2 and GFP-Rab8A (without DARPin E11) were incubated at 37 °C, 5% CO2 with either DMSO or MLi-2 (Tocris, catalog number 5756; 2 μM) for 1 h. Cells were washed twice with RT PBS one time and a final time with cold PBS.

Article Title: A designed ankyrin-repeat protein that targets Parkinson's disease-associated LRRK2.
Article Snippet: Please cite this article as: Dederer V, Sanz Murillo M, Karasmanis EP, Hatch KS, Chatterjee D, Preuss F, Abdul Azeez KR, Vu Nguyen L, Galicia C, Dreier B, Plückthun A, Versees W, Mathea S, Leschziner AE, Reck-Peterson SL, Knapp S, A designed ankyrin-repeat protein that targets Parkinson’s disease-associated LRRK2, Journal of Biological Chemistry (2024), doi: https://doi.org/10.1016/ j.jbc.2024.107469.

Western Blot:

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Expressing:

Article Title: Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome
Article Snippet: .. ECFP‐SKL (#54548), GFP‐Rab8A (#24898), GFP‐Rab8A‐Q67L (#24900), and tdTomato‐BicD2‐FKBP (#64205) expression vectors were purchased from Addgene. .. The PEX3‐GFP‐FRB expression vector for mammalian cells was constructed from PEX3‐GFP‐Halo (#67764; Addgene) and Mito‐mCherry‐FRB (#59352; Addgene) using PCR and the Gibson assembly kit (New England Biolabs).

Article Title: Endocytosis is regulated through the pH-dependent phosphorylation of Rab GTPases by Parkinson’s kinase LRRK2
Article Snippet: .. Reagents Mammalian expression vectors were obtained from the following sources: CFP-Rab5A 82; GFP-Rab5A 83; RFP-Rab5A 84; CFP-Rab5A(Q79L) 82; GFP-Rab5A(Q79L) (35140, Addgene); mCherry-Rab5A(Q79L) (35138, Addgene); CFP-Rab5A(S34N) 82; RFP-Rab7A 85; RFP-Rab7A(Q67L) 85, RFPRab7A(T22N) 85; GFP-Rab7A 86; GFP-Rab7A(Q67L) 86; GFP-Rab10A (49472, Addgene); GFP-Rab8A (24898, Addgene), R5BD-YFP 87, GFP-APPL1 88; GFP-RILPC33 36; RabGAP5-GFP 57; TBC1D2B-GFP (provided by Dr. Christian Rocheleau, McGill University); LRRK2-neonGreen (provided by Drs. Luis BonetPonce and Mark Cookson, National Institute of Health). .. Primary antibodies were purchased from the following vendors: LRRK2 (ab133474, Abcam) LRRK2 pS1292 (ab203181, Abcam); Rab10 (ab230260, Abcam), Rab10 pT73 (ab241060, Abcam); Rab8 (ab241061, Abcam), Rab8 pT72 (ab230260, Abcam), vinculin (MAB3574, Sigma-Aldrich); α-tubulin (T5168, Sigma-Aldrich).

Article Title: Endocytosis is regulated through the pH-dependent phosphorylation of Rab GTPases by Parkinson’s kinase LRRK2
Article Snippet: .. Mammalian expression vectors were obtained from the following sources: CFP-Rab5A ; GFP-Rab5A ; RFP-Rab5A ; CFP-Rab5A(Q79L) ; GFP-Rab5A(Q79L) (35140, Addgene); mCherry-Rab5A(Q79L) (35138, Addgene); CFP-Rab5A(S34N) ; RFP-Rab7A ; RFP-Rab7A(Q67L) , RFP-Rab7A(T22N) ; GFP-Rab7A ; GFP-Rab7A(Q67L) ; GFP-Rab10A (49472, Addgene); GFP-Rab8A (24898, Addgene), R5BD-YFP , GFP-APPL1 ; GFP-RILP C33 ; RabGAP5-GFP ; TBC1D2B-GFP (provided by Dr. Christian Rocheleau, McGill University); LRRK2-neonGreen (provided by Drs. Luis Bonet-Ponce and Mark Cookson, National Institute of Health). .. Primary antibodies were purchased from the following vendors: LRRK2 (ab133474, Abcam) LRRK2 pS1292 (ab203181, Abcam); Rab10 (ab230260, Abcam), Rab10 pT73 (ab241060, Abcam); Rab8 (ab241061, Abcam), Rab8 pT72 (ab230260, Abcam), vinculin (MAB3574, Sigma-Aldrich); α-tubulin (T5168, Sigma-Aldrich).

other:

Article Title: Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO
Article Snippet: The following plasmids described were purchased from Addgene: ARF1-GFP (39554), ARF1-ECFP (11381), ARF1(T31N)-ECFP (11384), ARF1(Q71L)-ECFP (11385), HA-ARF1 (10830), GFP-Rab11 (12674), mCherry UtrCH (26740), EMTB-mCherry (26742), GFP-RhoA (T19N; 12967), ARF6-CFP (11382), mCherry-Arp3 (27682), mCherry-Cortactin (27676), mCherry–clathrin light chain (27680), dsRed-Rab7 (12661), GFP-Rab8A (31803), and EMTB (ensconsin)-mCherry (26742).

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expression rrid addgene 232062 egfp rab8a rrid addgene 49543 gfp rab7 rrid addgene 12605 pqe30 darpin e11 n (Addgene inc)

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