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d gfp drd2  (Addgene inc)


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    Structured Review

    Addgene inc d gfp drd2
    D Gfp Drd2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+drd2/GFP-DRD2+(Plasmid+%2324099)/pm41642719-170-0-3
    Average 93 stars, based on 16 article reviews
    d gfp drd2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Manipulation of the Host Cell Membrane during Plasmodium Liver Stage Egress
    Article Snippet: HeLa cells (gift from Robert Menard, Pasteur Institute, Paris, France) were authenticated by short tandem repeat DNA profiling (Microsynth) and cultured as described before ( ). .. We transfected 2 × 10 6 HeLa cells with 4 μg of plasmid DNA of the following fluorescent reporter constructs with program T-28 in an Amaxa Nucleofector (Lonza): mCherry-utrophin ( ) (Addgene plasmid 26740), GFP-utrophin ( ) (Addgene plasmid 26737), GFP-C1-PLCdelta-PH ( ) (Addgene plasmid 21179), AKT-PH-GFP ( ) (Addgene plasmid 18836), Lact-C2-GFP ( ) (Addgene plasmid 22853), ezrin-GFP , ezrin(T567D)-GFP ( ) (Addgene plasmid 20681), E-cadherin–GFP ( ) (Addgene plasmid 28009), A2BR-YFP ( ) (Addgene plasmid 37202), GFP-DRD2 ( ) (Addgene plasmid 24099), PM-GFP ( ) (Addgene plasmid 21213), and GFP-GPI (TRAIL and DAF) ( ). .. Cells were subsequently used to seed glass bottom dishes (MatTek, In Vitro Scientific) and infected the next day with P. berghei sporozoites as previously described ( ).

    Plasmid Preparation:

    Article Title: Manipulation of the Host Cell Membrane during Plasmodium Liver Stage Egress
    Article Snippet: HeLa cells (gift from Robert Menard, Pasteur Institute, Paris, France) were authenticated by short tandem repeat DNA profiling (Microsynth) and cultured as described before ( ). .. We transfected 2 × 10 6 HeLa cells with 4 μg of plasmid DNA of the following fluorescent reporter constructs with program T-28 in an Amaxa Nucleofector (Lonza): mCherry-utrophin ( ) (Addgene plasmid 26740), GFP-utrophin ( ) (Addgene plasmid 26737), GFP-C1-PLCdelta-PH ( ) (Addgene plasmid 21179), AKT-PH-GFP ( ) (Addgene plasmid 18836), Lact-C2-GFP ( ) (Addgene plasmid 22853), ezrin-GFP , ezrin(T567D)-GFP ( ) (Addgene plasmid 20681), E-cadherin–GFP ( ) (Addgene plasmid 28009), A2BR-YFP ( ) (Addgene plasmid 37202), GFP-DRD2 ( ) (Addgene plasmid 24099), PM-GFP ( ) (Addgene plasmid 21213), and GFP-GPI (TRAIL and DAF) ( ). .. Cells were subsequently used to seed glass bottom dishes (MatTek, In Vitro Scientific) and infected the next day with P. berghei sporozoites as previously described ( ).

    Article Title: Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations
    Article Snippet: Pramipexole dihydrochloride monohydrate from TCI (Taipei, Taiwan). .. GFP-DRD2 was a gift from Jean-Michel Arrang (Addgene plasmid #24099, http://n2t.net/addgene:24099 , accessed on 25 December 2024; RRID: Addgene_24099) [ ]. ..

    Article Title: Disease-Associated Dopamine Receptor D2 Variants Exhibit Functional Consequences Depending on Different Heterotrimeric G-Protein Subunit Combinations.
    Article Snippet: Pramipexole dihydrochloride monohydrate from TCI (Taipei, Taiwan). .. GFP-DRD2 was a gift from Jean-Michel Arrang (Addgene plasmid #24099, http://n2t. net/addgene:24099, accessed on 25 December 2024; RRID: Addgene_24099) [36]. ..

    Article Title: Developing Photoaffinity Probes for Dopamine Receptor D 2 to Determine Targets of Parkinson’s Disease Drugs
    Article Snippet: A human codon-optimized DNA fragment of the Gqi5 chimera was synthesized (IDT Technologies) and amplified from the following primers: FORWARD 5′-AGCTGTACCCGGTCGCAATGACCCTGGAGAGCATCATGG-3′, REVERSE 5′-TGTGCGGGCAGGCAGAGTCAGAACAGGCCGCAGTCC-3′. .. A pcDNA3.1 vector was derived from GFP-DRD2 (Addgene #24099) linearized with the following primers: FORWARD 5′-AGGACTGCGGCCTGTTCTGACTCTGCTGCCTGCCCG-3′. ..

    Construct:

    Article Title: Manipulation of the Host Cell Membrane during Plasmodium Liver Stage Egress
    Article Snippet: HeLa cells (gift from Robert Menard, Pasteur Institute, Paris, France) were authenticated by short tandem repeat DNA profiling (Microsynth) and cultured as described before ( ). .. We transfected 2 × 10 6 HeLa cells with 4 μg of plasmid DNA of the following fluorescent reporter constructs with program T-28 in an Amaxa Nucleofector (Lonza): mCherry-utrophin ( ) (Addgene plasmid 26740), GFP-utrophin ( ) (Addgene plasmid 26737), GFP-C1-PLCdelta-PH ( ) (Addgene plasmid 21179), AKT-PH-GFP ( ) (Addgene plasmid 18836), Lact-C2-GFP ( ) (Addgene plasmid 22853), ezrin-GFP , ezrin(T567D)-GFP ( ) (Addgene plasmid 20681), E-cadherin–GFP ( ) (Addgene plasmid 28009), A2BR-YFP ( ) (Addgene plasmid 37202), GFP-DRD2 ( ) (Addgene plasmid 24099), PM-GFP ( ) (Addgene plasmid 21213), and GFP-GPI (TRAIL and DAF) ( ). .. Cells were subsequently used to seed glass bottom dishes (MatTek, In Vitro Scientific) and infected the next day with P. berghei sporozoites as previously described ( ).

    Amplification:

    Article Title: Developing Photoaffinity Probes for Dopamine Receptor D 2 to Determine Targets of Parkinson’s Disease Drugs
    Article Snippet: The transfer plasmid vector was derived from pHR-CMV-TetO2-IRES-mRuby2 (addgene #113885) linearized with the following primers: FORWARD 5′-TCCTGAAGATCCACTGCCTTGAGGTGCTGTTTCAGGG-3′, REVERSE 5′-CAGGACAGATTCAGTGGATCTTTCAGCTACGCAACCCATCAG-3′. .. The gene insert was amplified from GFP-DRD2 (addgene #24099) linearized with the following primers: FORWARD 5′-GATGGGTTGCGTAGCTGAAAGATCCACTGAATCTGTCCTGGTATGA-3′, REVERSE 5′-CCCTGAAACAGCACCTCAAGGCAGTGGAGGATCTTCAGGAAGG-3′. ..

    Over Expression:

    Article Title: Antipsychotic drugs elicit cytotoxicity in glioblastoma multiforme in a calcium‐dependent, non‐D 2 receptor‐dependent, manner
    Article Snippet: To generate these lines, commercially available DRD2 GIPZ‐shRNA viral particles (Dharmacon, Lafayette, CO, USA) or myc‐DDK tagged DRD2 lentiORF particles (Origene Technologies, Rockville, MD, USA) were used according to the manufacturer's recommendations, using 5 μg of particles (titer > 10 7 TU/mL) per 10 cm dish, followed by puromycin selection. .. Transient overexpression with GFP‐ DRD2 [Addgene #24099 ] for Figure was achieved with Lipofectamine 3000 according to the manufacturer's recommendations, using the higher recommended concentrations of lipofectamine. ..

    Article Title: Antipsychotic drugs elicit cytotoxicity in glioblastoma multiforme in a calcium-dependent, non-D 2 receptor-dependent, manner.
    Article Snippet: To generate these lines, commercially available DRD2 GIPZ-shRNA viral particles (Dharmacon, Lafayette, CO, USA) or myc-DDK tagged DRD2 lentiORF particles (Origene Technologies, Rockville, MD, USA) were used according to the manufacturer's recommendations, using 5 μg of particles (titer > 107 TU/mL) per 10 cm dish, followed by puromycin selection. .. Transient overexpression with GFP-DRD2 [Addgene #2409911] for Figure S3 was achieved with Lipofectamine 3000 according to the manufacturer's recommendations, using the higher recommended concentrations of lipofectamine. ..

    Derivative Assay:

    Article Title: Developing Photoaffinity Probes for Dopamine Receptor D 2 to Determine Targets of Parkinson’s Disease Drugs
    Article Snippet: A human codon-optimized DNA fragment of the Gqi5 chimera was synthesized (IDT Technologies) and amplified from the following primers: FORWARD 5′-AGCTGTACCCGGTCGCAATGACCCTGGAGAGCATCATGG-3′, REVERSE 5′-TGTGCGGGCAGGCAGAGTCAGAACAGGCCGCAGTCC-3′. .. A pcDNA3.1 vector was derived from GFP-DRD2 (Addgene #24099) linearized with the following primers: FORWARD 5′-AGGACTGCGGCCTGTTCTGACTCTGCTGCCTGCCCG-3′. ..



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