Journal: RSC Advances

Article Title: Evaluation of a GDNF-eluting nanofibrous PCL conduit in a mouse model of peripheral nerve injury

doi: 10.1039/d6ra03291e

Figure Lengend Snippet: YFP+ axon regeneration through nerve repair constructs. (A) Representative fluorescence images of YFP+ axons regenerating through either nerve grafts or PCL or PCL + GDNF conduits ( n = 6 per group). (B) Box and whisker plots show median, interquartile range (box), and the minimum and maximum values (whiskers) of total axon counts. (C) Axon counts were normalised to the proximal stump and expressed as a percentage (mean ± SD). Data were analysed with two-way ANOVA with Tukey's post hoc test p < 0.05 (*), p < 0.01 (**) (Scale bars = 200 µm).

Article Snippet: The endings were trimmed to create a gap of 3 mm between the proximal and distal stumps, and either a PCL or PCL + GDNF conduit was sutured end-to-end to the stumps using four 9–0 monofilament polyamide sutures (Ethilon®; Ethicon Ltd, England).

Techniques: Construct, Fluorescence, Whisker Assay

Journal: RSC Advances

Article Title: Evaluation of a GDNF-eluting nanofibrous PCL conduit in a mouse model of peripheral nerve injury

doi: 10.1039/d6ra03291e

Figure Lengend Snippet: β-III tubulin+ axon regeneration through nerve repair constructs. (A) Representative fluorescence images of TUBB3+ axons regenerating through either nerve grafts or PCL or PCL + GDNF conduits ( n = 6 per group). (B) Box and whisker plots show median, interquartile range (box), and the minimum and maximum values (whiskers) of total axon counts. (C) Axon counts were normalised to the proximal stump and expressed as a percentage (mean ± SD). Data were analysed with two-way ANOVA with Tukey's post hoc test. p < 0.01 (**) (Scale bars = 200 µm).

Article Snippet: The endings were trimmed to create a gap of 3 mm between the proximal and distal stumps, and either a PCL or PCL + GDNF conduit was sutured end-to-end to the stumps using four 9–0 monofilament polyamide sutures (Ethilon®; Ethicon Ltd, England).

Techniques: Construct, Fluorescence, Whisker Assay

Journal: RSC Advances

Article Title: Evaluation of a GDNF-eluting nanofibrous PCL conduit in a mouse model of peripheral nerve injury

doi: 10.1039/d6ra03291e

Figure Lengend Snippet: Schwann cell presence within nerve repair constructs. (A) Representative fluorescence images of S100B+ Schwann cells within nerve grafts or PCL or PCL + GDNF conduits. Cells migrated into fibrin hydrogel-filled spaces between nanofibrous mat folds. (B) S100B+ fluorescent area (µm 2 ) for Schwann cell quantification. (C) Fluorescent area was normalised to the entire measured area and expressed as a percentage (mean ± SD). Data were analysed by two-way ANOVA with Tukey's multiple comparisons test. Asterisks indicate significant differences between groups within the same segment ( p < 0.05 (*)), and all such comparisons are shown. Hash symbols ( p < 0.05 (#)) indicate significant differences between segments within the same group, and all such comparisons are shown, except that comparisons involving proximal segments are omitted. (Scale bars = 200 µm).

Article Snippet: The endings were trimmed to create a gap of 3 mm between the proximal and distal stumps, and either a PCL or PCL + GDNF conduit was sutured end-to-end to the stumps using four 9–0 monofilament polyamide sutures (Ethilon®; Ethicon Ltd, England).

Techniques: Construct, Fluorescence