Journal: Cell Reports Medicine

Article Title: Autoantibodies against NCAM1 from patients with schizophrenia cause schizophrenia-related behavior and changes in synapses in mice

doi: 10.1016/j.xcrm.2022.100597

Figure Lengend Snippet:

Article Snippet: GDNF Human Recombinant Protein , Origene , Cat# TP760516.

Techniques: Plasmid Preparation, Recombinant, Labeling, Enzyme-linked Immunosorbent Assay, Software

Journal: Bioactive Materials

Article Title: Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

doi: 10.1016/j.bioactmat.2022.03.014

Figure Lengend Snippet: Evaluation of the cytotoxicity of the ANXs scaffold in vitro. (A, D) Living/dead double staining of Schwann cells grown on the ANXs scaffold for 3 days and 7 days (live: green, dead: red). (B, E) SEM images of Schwann cells growing on CD SD and CD + scCO 2 NG scaffolds for 7 days (the picture on the right is an enlarged view of the yellow area in the picture on the left). (C, F) Immunofluorescence images of Schwann cells growing on CD SD and CD + scCO 2 NG scaffolds for 7 days, respectively (S100: red, nucleus: blue). (G) Quantification of the number of live/dead double-stained Schwann cells in each region (0.36 mm 2 ). Data are presented as the mean ± SD (n = 3). (H) The CCK-8 assay was performed after 1, 3, 5 and 7 days of cell culture. Data are presented as the mean ± SD (n = 5). (I, J) Quantitative analysis of the GDNF and NGF expression levels of Schwann cells on the ANXs scaffold. Data are presented as the mean ± SD (n = 5). Statistical analysis: n.s. no significances, **p < 0.01, *p < 0.05.

Article Snippet: In brief, the medium of each group was centrifuged at 1500 rpm and 4 °C for 10 min, the concentration of NGF and BDNF in the supernatant was assessed using ELISA kits, the rat GDNF ELISA kit (EK0363, BOSTER, China) and the rat NGF/NGFβ ELISA kit (EK0471, BOSTER, China), and the absorbance of each well at 450 nm was determined using a spectrophotometer (EPOCH TAKE 3, Bio-Tek, USA).

Techniques: In Vitro, Double Staining, Immunofluorescence, Staining, CCK-8 Assay, Cell Culture, Expressing

Journal: Neural Plasticity

Article Title: Behavioral Improvement and Regulation of Molecules Related to Neuroplasticity in Ischemic Rat Spinal Cord Treated with PEDF

doi: 10.1155/2014/451639

Figure Lengend Snippet: Neurotrophic factors, Eph receptors, and GTPase levels.

Article Snippet: The gene expression of the following molecules was analyzed: the neurotrophic factors NT-3 (Rn00579280_m1), GDNF (Rn00569510_m1), BDNF (customized, Forward 5′TGGTTATTTCATACTTCGG TTGCATGA3′, Reverse 5′TGTCCGTGGACGTTTGCTT3′, and Probe 5′CTGCGCCCATGAAAG3′), and FGF-2 (Rn00570809_m1), the Eph receptors EphA6 (Rn01474859_m1) and EphB2 (Rn01181017_m1), and the small GTPase RhoA (Rn04219610_g1).

Techniques: Saline

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Cell Culture, Quantitative RT-PCR, Western Blot

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Sinomenine regulates the cholinergic anti-inflammatory pathway to inhibit TLR4/NF-κB pathway and protect the homeostasis in brain and gut in scopolamine-induced Alzheimer's disease mice.

doi: 10.1016/j.biopha.2024.116190

Figure Lengend Snippet: Fig. 6. Effects of SIN on intestinal mucosal barrier in SCOP-induced mice. A-B. Images of ileum tissue sections immunostained for ZO-1 (A) and Occludin (B) captured with a fluorescence microscope at × 200 magnification, bar = 100 µm. C-D. Fluorescence quantitative statistics for ZO-1(C) and Occludin (D). E. The expressions of ZO-1, Occludin and GDNF in the ileum tissue of each group detected by western blot. F. Gray quantitative analysis of western blot bands for ZO-1, Occludin and GDNF. The data are expressed as mean ± SD (n = 4 each group), #P < 0.05 verse control group, *P < 0.05 verse SCOP group.

Article Snippet: Monoclonal anti-GDNF antibody (#SC-13147) was purchased from Santa Cruz Biotechnology (Texas, USA) Monoclonal antibodies against NF-κB p65 (#8242 S), p-NF-κB (#3033 S), IκBα (#4814 S), p-IκBα (#9246 S) and Occludin (#91131 S) were purchased from Cell Signaling Technology (Massachusetts, USA).

Techniques: Fluorescence, Microscopy, Western Blot, Control