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glyceraldehyde 3 phosphate dehydrogenase  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc glyceraldehyde 3 phosphate dehydrogenase
    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = <t>glyceraldehyde</t> <t>3</t> phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.
    Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 12304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh/GAPDH+Rabbit+mAb/pmc12816905-99-91-97
    Average 99 stars, based on 12304 article reviews
    glyceraldehyde 3 phosphate dehydrogenase - by Bioz Stars, 2026-11
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    Images

    1) Product Images from "Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice"

    Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice

    Journal: Journal of Sport and Health Science

    doi: 10.1016/j.jshs.2025.101100

    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.
    Figure Legend Snippet: Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Techniques Used: Phospho-proteomics, Injection, Western Blot, Muscles, Ubiquitin Proteomics, Staining, Binding Assay

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    Western Blot:

    Article Title: Drug Repurposing for Treating NCOR‐Related Intellectual Disability
    Article Snippet: .. For Western blot, total cell lysates or brain tissue lysates were resolved by Tris‐glycine SDS‐PAGE, transferred to polyvinylidene difluoride (PVDF) membranes, and blotted with antibodies against Flag (F1804, MBL), β‐actin (81115‐1‐RR, Proteintech), Nrg1 (A0687, Abclonal), NCOR1 (A301‐146A, Bethyl), GAPDH (#2118L, CST), and Hsp90(4874S, CST). .. After incubation with the appropriate secondary antibodies, the membranes are exposed using the Dual‐ECL Kit (FD8020, FuDe) and a Tanon 3500 Gel Imaging System.

    Tissue:

    Article Title: Drug Repurposing for Treating NCOR‐Related Intellectual Disability
    Article Snippet: .. For Western blot, total cell lysates or brain tissue lysates were resolved by Tris‐glycine SDS‐PAGE, transferred to polyvinylidene difluoride (PVDF) membranes, and blotted with antibodies against Flag (F1804, MBL), β‐actin (81115‐1‐RR, Proteintech), Nrg1 (A0687, Abclonal), NCOR1 (A301‐146A, Bethyl), GAPDH (#2118L, CST), and Hsp90(4874S, CST). .. After incubation with the appropriate secondary antibodies, the membranes are exposed using the Dual‐ECL Kit (FD8020, FuDe) and a Tanon 3500 Gel Imaging System.

    Control:

    Article Title: The role of rapamycin in neural tube defects: insights from bioinformatics analysis and experimental validation.
    Article Snippet: Neural tube defects (NTDs) are the most severe congenital malformations of the central nervous system.. Fetal developmental homeostasis disruptions heighten NTDs risk through aberrant maternal-fetal signaling and periconceptional medication exposure.. Rapamycin, an inhibitor of the mTOR signaling pathway, is a widely used clinical drug whose effects on embryonic neural development remain unclear.

    Article Title: Dynamin-2 regulates polarized endometrial secretion and epithelial adhesion.
    Article Snippet: .. Membranes were blocked with 5% skim milk for 1 h at RT 178 and incubated overnight at 4 °C with primary antibodies against DNM2 (0.1 μg/mL, Sigma, 179 HPA054246) or GAPDH (1:2000, 2118, loading control, Cell Signalling, Danvers, MA, USA). .. 180 Following three washes in 0.1% TBS-Tween, membranes were incubated with HRP181 conjugated secondary antibody (0.25 μg/mL, Dako, P0448) for 1 h at RT.

    Expressing:

    Article Title: The role of rapamycin in neural tube defects: insights from bioinformatics analysis and experimental validation.
    Article Snippet: Neural tube defects (NTDs) are the most severe congenital malformations of the central nervous system.. Fetal developmental homeostasis disruptions heighten NTDs risk through aberrant maternal-fetal signaling and periconceptional medication exposure.. Rapamycin, an inhibitor of the mTOR signaling pathway, is a widely used clinical drug whose effects on embryonic neural development remain unclear.

    Incubation:

    Article Title: Integrated Transcriptomic Analysis Identifies ENO3 as a Candidate Lactate Metabolism‐Associated Biomarker in Colorectal Cancer Metastasis
    Article Snippet: Samples (30 μg per lane) were resolved by SDS‐PAGE, transferred onto PVDF membranes (Vazyme #E801‐01), and blocked with 5% non‐fat milk in TBST for 1 h at room temperature. .. Membranes were probed overnight at 4°C with antibodies against ENO3 (1:1000, Invitrogen # MA563785 ) and GAPDH (1:3000, CST #2118S), followed by 1‐h incubation with HRP‐conjugated secondary antibodies (1:5000, CST #7074S). .. Chemiluminescent signals were developed using ECL substrate (Vazyme #E423‐01) and captured on a Bio‐Rad ChemiDoc imaging system.

    Article Title: Dynamin-2 regulates polarized endometrial secretion and epithelial adhesion.
    Article Snippet: .. Membranes were blocked with 5% skim milk for 1 h at RT 178 and incubated overnight at 4 °C with primary antibodies against DNM2 (0.1 μg/mL, Sigma, 179 HPA054246) or GAPDH (1:2000, 2118, loading control, Cell Signalling, Danvers, MA, USA). .. 180 Following three washes in 0.1% TBS-Tween, membranes were incubated with HRP181 conjugated secondary antibody (0.25 μg/mL, Dako, P0448) for 1 h at RT.

    Article Title: Deficiency of purinergic receptor P2X7 attenuates neointima hyperplasia in mice after carotid artery wire injury.
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore). .. The membranes were incubated with the following primary antibodies: P2X7 (Alome lab, APR-004, 1:1000), NLRP3 (CST, 15101S, 1:1000), GSDMD (Abcam, ab209845, 1:1000), and GAPDH (CST, 2118S, 1:1000). .. After incubation with the primary antibodies, the membranes were washed and incubated with HRP-conjugated secondary antibody (Santa Cruz) for 1 h at room temperature.



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    ( A ) Pie chart showing the proportion and number of methylated1 and escapee1 5-kb bins from TE to the first-trimester CTB. ( B ) Pie chart showing the proportion and number of methylated2 and escapee2 5-kb bins from the first- to third-trimester CTB. ( C ) Box plots showing the DNA methylation, H3K9me3, H3K9ac, and expression levels of genes and repeats undergoing methylation (genes, 25,044; repeats, 3,799,552) or escapee (genes, 10,225; repeats, 232,372) in the first-trimester CTB from TE to the first trimester. Significance between the groups was evaluated by Student’s t test. ( D ) GO enrichment analysis for methylated1 and escapee1 genes from TE to the first-trimester CTB. ( E ) qRT-PCR analysis of representative methylated1 or escapee1 genes in human embryonic stem cell (hESC), hTSC, and the <t>first-trimester</t> <t>CTB.</t> <t>Glyceraldehyde-3-phosphate</t> dehydrogenase ( GAPDH ) is set as an endogenous control. Data are presented as means ± SD (three biological replicates). ( F ) Box plots showing the DNA methylation, H3K9me3, H3K9ac, and expression levels of genes and repeats undergoing methylation (genes, 22,098; repeats, 2,847,005) or escapee (genes, 15,384; repeats, 1,217,495) in the third-trimester CTB from the first to third trimesters. Significance between the groups was evaluated by Student’s t test.
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    Image Search Results


    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Journal: Journal of Sport and Health Science

    Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice

    doi: 10.1016/j.jshs.2025.101100

    Figure Lengend Snippet: Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Article Snippet: Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4(COX4; #11967; Cell Signaling Technologies), Akt substrate 160 (AS160 S318; #8619; Cell Signaling Technologies), AS160 T642 (#8881; Cell Signaling Technologies), eukaryotic translation initiation factor 4E binding protein (4E-BP1; #9452; Cell Signaling Technologies), and glyceraldehyde 3 phosphate dehydrogenase (GAPDH; #2118; Cell Signaling Technologies).

    Techniques: Phospho-proteomics, Injection, Western Blot, Muscles, Ubiquitin Proteomics, Staining, Binding Assay

    ( A ) Pie chart showing the proportion and number of methylated1 and escapee1 5-kb bins from TE to the first-trimester CTB. ( B ) Pie chart showing the proportion and number of methylated2 and escapee2 5-kb bins from the first- to third-trimester CTB. ( C ) Box plots showing the DNA methylation, H3K9me3, H3K9ac, and expression levels of genes and repeats undergoing methylation (genes, 25,044; repeats, 3,799,552) or escapee (genes, 10,225; repeats, 232,372) in the first-trimester CTB from TE to the first trimester. Significance between the groups was evaluated by Student’s t test. ( D ) GO enrichment analysis for methylated1 and escapee1 genes from TE to the first-trimester CTB. ( E ) qRT-PCR analysis of representative methylated1 or escapee1 genes in human embryonic stem cell (hESC), hTSC, and the first-trimester CTB. Glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) is set as an endogenous control. Data are presented as means ± SD (three biological replicates). ( F ) Box plots showing the DNA methylation, H3K9me3, H3K9ac, and expression levels of genes and repeats undergoing methylation (genes, 22,098; repeats, 2,847,005) or escapee (genes, 15,384; repeats, 1,217,495) in the third-trimester CTB from the first to third trimesters. Significance between the groups was evaluated by Student’s t test.

    Journal: Science Advances

    Article Title: Lineage-specific and gestational age–dependent DNA methylation programming orchestrates human placental development

    doi: 10.1126/sciadv.aee0560

    Figure Lengend Snippet: ( A ) Pie chart showing the proportion and number of methylated1 and escapee1 5-kb bins from TE to the first-trimester CTB. ( B ) Pie chart showing the proportion and number of methylated2 and escapee2 5-kb bins from the first- to third-trimester CTB. ( C ) Box plots showing the DNA methylation, H3K9me3, H3K9ac, and expression levels of genes and repeats undergoing methylation (genes, 25,044; repeats, 3,799,552) or escapee (genes, 10,225; repeats, 232,372) in the first-trimester CTB from TE to the first trimester. Significance between the groups was evaluated by Student’s t test. ( D ) GO enrichment analysis for methylated1 and escapee1 genes from TE to the first-trimester CTB. ( E ) qRT-PCR analysis of representative methylated1 or escapee1 genes in human embryonic stem cell (hESC), hTSC, and the first-trimester CTB. Glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) is set as an endogenous control. Data are presented as means ± SD (three biological replicates). ( F ) Box plots showing the DNA methylation, H3K9me3, H3K9ac, and expression levels of genes and repeats undergoing methylation (genes, 22,098; repeats, 2,847,005) or escapee (genes, 15,384; repeats, 1,217,495) in the third-trimester CTB from the first to third trimesters. Significance between the groups was evaluated by Student’s t test.

    Article Snippet: The membranes were blocked with protein-free rapid blocking buffer and next incubated with EED (85322S, Cell Signaling Technology), EZH2 (39901, Active motif), SUZ12 (3737S, Cell Signaling Technology), H3K9me3 (39161, Active motif), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 60004-1-Ig, Proteintech), H3K27me3 (C15410195, Diagenode), H3 (4499, Cell Signaling Technology), CDH1 (3195S, Cell Signaling Technology), CDH2 (ab18203, Abcam), EpCAM (MA5-12436, Thermo Fisher Scientific), MMP2 (40994S, Cell Signaling Technology), and DNMT1 (24206-1-AP, Proteintech) overnight at 4°C on a shaker.

    Techniques: DNA Methylation Assay, Expressing, Methylation, Analysis, Quantitative RT-PCR, Stem Cell, Control, Significance Assay