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loading control  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc loading control
    Loading Control, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 11415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh+loading+control/GAPDH+XP+Rabbit+mAb/pm41820865-125-9-12
    Average 99 stars, based on 11415 article reviews
    loading control - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Control:

    Article Title: Fluorescence Lifetime Imaging Enables In Vivo Quantification of PD-L1 Expression and Intertumoral Heterogeneity
    Article Snippet: Then, they were probed with a primary antibody against PD-L1 (1:1,000, Abcam, ab213480) overnight followed by incubation with an anti-rabbit horseradish peroxidase secondary antibody (1:3,000, Cell Signaling Technology, 7074S) for 1 hour at room temperature. .. GAPDH was used as a loading control (1:5,000, Cell Signaling Technology, 2118S). .. Protein bands were detected by Clarity Western ECL Substrate (Bio-Rad) according to the manufacturer’s instructions and developed by autoradiography film (Lab Scientific bioKEMIX, Inc., cat# XAR ALF 2025).

    Article Title: CFTR-modulating compositions and methods
    Article Snippet: Expression of the Cas9-RT gene editing polypeptide in liver was measured by Western blot where Cas9 was detected by a mouse monoclonal antibody (7A9-3A3, Cell Signaling Technology) and GAPDH (Cell Signaling Technology) was used as a loading control. (FIG. 8). .. Expression of the Cas9-RT gene editing polypeptide in liver was measured by Western blot where Cas9 was detected by a mouse monoclonal antibody (7A9-3A3, Cell Signaling Technology) and GAPDH (Cell Signaling Technology) was used as a loading control. (FIG. 8). .. TTR protein levels in serum were quantified by an ELISA using a standard curve (Aviva Biosciences).

    Article Title: Integrated effects of altered action potentials and calcium release on skeletal muscle force generation in transgenic Huntington’s disease mice
    Article Snippet: .. Thus, the densitometry values were normalized to the GAPDH loading control (Cell Signaling Technologies, 2118, 1:1000), densitometry values were obtained from the same membrane. .. Primary antibodies that have been successfully used previously were selected and include SERCA1 (Thermo Fisher Scientific, MA3-912, 1:1000) [ , , ], SERCA2 (Thermo Fisher Scientific, MA3-919, 1:1000) [ , ], parvalbumin (Thermo Fisher Scientific, PA1-933, 1:10000) [ ], and troponin C (Santa Cruz, sc-48347, 1:500) [ ].

    Article Title: Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferation.
    Article Snippet: Primary antibody: anti-NHE1 (Santa Cruz Biotechnology; sc-136239; 1:1000), anti-Piezo1 (Invitrogen; PA5-77617; 1:1000), anti-Phospho-p44/42 MAPK (p-ERK1/2; Cell Signaling; 9101; 1:1000), anti-p44/42 MAPK (ERK1/2; Cell Signaling; 4695; 1:1000). .. GAPDHwas used as a loading control (Cell Signaling; 2118; 1:4000). .. Secondary Antibody: anti-mouse IgG, HRP-linked antibody (Cell Signaling; 7076; 1:1000), and anti-rabbit IgGHRP-linked antibody (Cell Signaling, 7074S; 1:1000).

    Article Title: Integrated effects of altered action potentials and calcium release on skeletal muscle force generation in transgenic Huntington's disease mice.
    Article Snippet: .. Thus, the densitometry values were normalized to the GAPDH loading control (Cell Signaling Technologies, 2118, 1:1000), densitometry values were obtained from the same membrane. .. Primary antibodies that have been successfully used previously were selected and include SERCA1 (Thermo Fisher Scientific, MA3-912, 1:1000) [12, 26, 39], SERCA2 (Thermo Fisher Scientific, MA3-919, 1:1000) [13, 76], parvalbumin (Thermo Fisher Scientific, PA1-933, 1:10000) [47], and troponin C (Santa Cruz, sc-48347, 1:500) [40].

    Expressing:

    Article Title: CFTR-modulating compositions and methods
    Article Snippet: Expression of the Cas9-RT gene editing polypeptide in liver was measured by Western blot where Cas9 was detected by a mouse monoclonal antibody (7A9-3A3, Cell Signaling Technology) and GAPDH (Cell Signaling Technology) was used as a loading control. (FIG. 8). .. Expression of the Cas9-RT gene editing polypeptide in liver was measured by Western blot where Cas9 was detected by a mouse monoclonal antibody (7A9-3A3, Cell Signaling Technology) and GAPDH (Cell Signaling Technology) was used as a loading control. (FIG. 8). .. TTR protein levels in serum were quantified by an ELISA using a standard curve (Aviva Biosciences).

    Western Blot:

    Article Title: CFTR-modulating compositions and methods
    Article Snippet: Expression of the Cas9-RT gene editing polypeptide in liver was measured by Western blot where Cas9 was detected by a mouse monoclonal antibody (7A9-3A3, Cell Signaling Technology) and GAPDH (Cell Signaling Technology) was used as a loading control. (FIG. 8). .. Expression of the Cas9-RT gene editing polypeptide in liver was measured by Western blot where Cas9 was detected by a mouse monoclonal antibody (7A9-3A3, Cell Signaling Technology) and GAPDH (Cell Signaling Technology) was used as a loading control. (FIG. 8). .. TTR protein levels in serum were quantified by an ELISA using a standard curve (Aviva Biosciences).

    Membrane:

    Article Title: Integrated effects of altered action potentials and calcium release on skeletal muscle force generation in transgenic Huntington’s disease mice
    Article Snippet: .. Thus, the densitometry values were normalized to the GAPDH loading control (Cell Signaling Technologies, 2118, 1:1000), densitometry values were obtained from the same membrane. .. Primary antibodies that have been successfully used previously were selected and include SERCA1 (Thermo Fisher Scientific, MA3-912, 1:1000) [ , , ], SERCA2 (Thermo Fisher Scientific, MA3-919, 1:1000) [ , ], parvalbumin (Thermo Fisher Scientific, PA1-933, 1:10000) [ ], and troponin C (Santa Cruz, sc-48347, 1:500) [ ].

    Article Title: Integrated effects of altered action potentials and calcium release on skeletal muscle force generation in transgenic Huntington's disease mice.
    Article Snippet: .. Thus, the densitometry values were normalized to the GAPDH loading control (Cell Signaling Technologies, 2118, 1:1000), densitometry values were obtained from the same membrane. .. Primary antibodies that have been successfully used previously were selected and include SERCA1 (Thermo Fisher Scientific, MA3-912, 1:1000) [12, 26, 39], SERCA2 (Thermo Fisher Scientific, MA3-919, 1:1000) [13, 76], parvalbumin (Thermo Fisher Scientific, PA1-933, 1:10000) [47], and troponin C (Santa Cruz, sc-48347, 1:500) [40].

    other:


    Article Title: Proteomic signature of menstrual blood mesenchymal stromal cells and their extracellular vesicles in women with unexplained infertility
    Article Snippet: 25 Research question: Can the proteome of menstrual blood mesenchymal stem cells, their 26 extracellular vesicles (EVs), or EV-depleted supernatant be employed for personalized 27 identification of altered cellular processes in women with unexplained infertility (uIF), 28 enabling their stratification into etiopathogenetic endotypes?. 29 Design: For MenSCs isolation, menstrual blood was collected from 12 fertile healthy 30 volunteers (control) and 8 patients with uIF.. MenSC EVs were isolated using Iodixanol 31 Density Gradient Centrifugation, quantified by flow cytometry and characterized according 32 to MISEV guidelines by TEM, flow cytometry and Western blot.

    Article Title: Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferation
    Article Snippet: Western blots were performed using protocols previously described in Mistriotis et al., using NuPAGE 4–12% Bis-Tris Protein Gels (Thermo Fischer Scientific, NP0336BOX) in Invitrogen Novex NuPage MES SDS Running Buffer (1X, Thermo Fisher Scientific, NP0002).



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    PDT induces autophagic flux in nasopharyngeal carcinoma cells. A This panel shows representative western blots of <t>LC3-II,</t> <t>p62/SQSTM1,</t> and <t>GAPDH</t> in C666-1 and HONE1 cells, respectively, treated with PDT alone or in combination with rapamycin (autophagy activation) or chloroquine (autophagy inhibition). B This panel shows densitometric quantification of LC3-II and p62 normalized to GAPDH, respectively, presented as mean ± SD from independent experiments performed separately in each cell line. P ***-value < 0.001
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    Image Search Results


    A subset of novel k-FATs exhibits yeast toxicity and IcsB-like fatty acid acyltransferase activity. ( A ) Map of the pRS313 Gal1p2 centromeric plasmid used for the galactose-inducible expression of IcsB and its homologs. ( B )The expression of IcsB and seven of its homologs in the presence of galactose inhibits yeast growth compared to the empty vector (EV) control. The glucose plate indicates that there is no growth inhibition in the absence of expression and that similar amounts of each strain were plated. Four dilutions were plated (non-diluted, 1/10, 1/100, and 1/1,000). The graph represents the quantification of growth on three independent replicates of the 1/1,000 dilution. The error bars represent the standard deviation of the mean. A Student’s t-test with unpaired data and a 95% CI was performed (*, P < 0.05; **, P < 0.1, ***, P < 0.01). ( C ) Protein acylation in the membrane protein fraction of yeast expressing IcsB and its homologs according to the in-gel fluorescence acyltransferase assay (upper panels). -Alk IcsB is the IcsB sample prior to the addition of Alk-16. -Alk IcsB, Alk-16 EV, and Alk-16 IcsB were loaded on both gels to serve as controls. This acylation assay was performed using the strains from B that were induced with galactose for six hours in the presence of Alk-16 for the final 4 h. The total protein in these samples were assessed using the TGX stain (bottom panels). ( D ) The expression of IcsB and of its homologs was assessed by immunoblotting with an antibody binding to their 3× FLAG tag and with the GAPDH as a loading control.

    Journal: mBio

    Article Title: A bacterial family of fatty acid acyltransferases related to the Shigella effector IcsB

    doi: 10.1128/mbio.03890-25

    Figure Lengend Snippet: A subset of novel k-FATs exhibits yeast toxicity and IcsB-like fatty acid acyltransferase activity. ( A ) Map of the pRS313 Gal1p2 centromeric plasmid used for the galactose-inducible expression of IcsB and its homologs. ( B )The expression of IcsB and seven of its homologs in the presence of galactose inhibits yeast growth compared to the empty vector (EV) control. The glucose plate indicates that there is no growth inhibition in the absence of expression and that similar amounts of each strain were plated. Four dilutions were plated (non-diluted, 1/10, 1/100, and 1/1,000). The graph represents the quantification of growth on three independent replicates of the 1/1,000 dilution. The error bars represent the standard deviation of the mean. A Student’s t-test with unpaired data and a 95% CI was performed (*, P < 0.05; **, P < 0.1, ***, P < 0.01). ( C ) Protein acylation in the membrane protein fraction of yeast expressing IcsB and its homologs according to the in-gel fluorescence acyltransferase assay (upper panels). -Alk IcsB is the IcsB sample prior to the addition of Alk-16. -Alk IcsB, Alk-16 EV, and Alk-16 IcsB were loaded on both gels to serve as controls. This acylation assay was performed using the strains from B that were induced with galactose for six hours in the presence of Alk-16 for the final 4 h. The total protein in these samples were assessed using the TGX stain (bottom panels). ( D ) The expression of IcsB and of its homologs was assessed by immunoblotting with an antibody binding to their 3× FLAG tag and with the GAPDH as a loading control.

    Article Snippet: Primary antibodies used were mouse FLAG-tag (Sigma-Aldrich, F3165), mouse Myc-tag (Genescript, A00704), and rabbit GAPDH (Bioss antibodies, BS-8789R) diluted 1/5,000, 1/1,000, and 1/10,000, respectively.

    Techniques: Activity Assay, Plasmid Preparation, Expressing, Control, Inhibition, Standard Deviation, Membrane, Fluorescence, Staining, Western Blot, Binding Assay, FLAG-tag

    Mutations in the catalytic site of k-FATs abolish their enzymatic activity. ( A ) Yeast growth inhibition tests of catalytic residues mutants H145A (left), D195A (center), and C306A (right) compared to their WT counterpart in IcsB and in toxic homologs. The graph represents the quantification of growth on three independent replicates with the dilution of yeast cultures 1/1,000. The error bars represent the standard deviation from the mean. A Student’s t -test with unpaired data and a 95% CI was performed (*, P < 0.05; **, P < 0.1, ***, P < 0.01). ( B ) In-gel protein acylation of C306A mutants compared to their WT counterparts (upper panels). The total protein in these samples were assessed using the TGX stain (bottom panels). ( C ) The expression of IcsB and toxic homolog C306A mutants was compared to their WT counterparts by immunoblotting with an antibody binding to their 3×FLAG tag and with the GAPDH as a loading control.

    Journal: mBio

    Article Title: A bacterial family of fatty acid acyltransferases related to the Shigella effector IcsB

    doi: 10.1128/mbio.03890-25

    Figure Lengend Snippet: Mutations in the catalytic site of k-FATs abolish their enzymatic activity. ( A ) Yeast growth inhibition tests of catalytic residues mutants H145A (left), D195A (center), and C306A (right) compared to their WT counterpart in IcsB and in toxic homologs. The graph represents the quantification of growth on three independent replicates with the dilution of yeast cultures 1/1,000. The error bars represent the standard deviation from the mean. A Student’s t -test with unpaired data and a 95% CI was performed (*, P < 0.05; **, P < 0.1, ***, P < 0.01). ( B ) In-gel protein acylation of C306A mutants compared to their WT counterparts (upper panels). The total protein in these samples were assessed using the TGX stain (bottom panels). ( C ) The expression of IcsB and toxic homolog C306A mutants was compared to their WT counterparts by immunoblotting with an antibody binding to their 3×FLAG tag and with the GAPDH as a loading control.

    Article Snippet: Primary antibodies used were mouse FLAG-tag (Sigma-Aldrich, F3165), mouse Myc-tag (Genescript, A00704), and rabbit GAPDH (Bioss antibodies, BS-8789R) diluted 1/5,000, 1/1,000, and 1/10,000, respectively.

    Techniques: Activity Assay, Inhibition, Standard Deviation, Staining, Expressing, Western Blot, Binding Assay, Control

    Relative mRNA expression levels of CALR, SMC4, P53, MCM10 and SRCIN1, which were validated by Q-PCR analysis normalized to GAPDH. The data are expressed as mean ± SD. **: p < 0.01 (the androgen-activated AP vs. the androgen-unactivated AP, n = 3).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: The regulation of pedicle initiation by androgens in sika deer (Cervus nippon)

    doi: 10.3389/fcell.2025.1708732

    Figure Lengend Snippet: Relative mRNA expression levels of CALR, SMC4, P53, MCM10 and SRCIN1, which were validated by Q-PCR analysis normalized to GAPDH. The data are expressed as mean ± SD. **: p < 0.01 (the androgen-activated AP vs. the androgen-unactivated AP, n = 3).

    Article Snippet: Antibodies used in the Western blot analysis included: anti-GAPDH-loading control monoclonal antibody (bsm-33033M, Bioss, China), anti-SRCIN1 antibody (D153560, BBI life sciences, China), anti-Calreticulin polyclonal antibody (bs-5913R, Bioss, China), anti-SMC4 polyclonal antibody (bs-7728R, Bioss, China), anti-p53 protein (wt-p53) polyclonal antibody (bs-0033R, Bioss, China).

    Techniques: Expressing

    PDT induces autophagic flux in nasopharyngeal carcinoma cells. A This panel shows representative western blots of LC3-II, p62/SQSTM1, and GAPDH in C666-1 and HONE1 cells, respectively, treated with PDT alone or in combination with rapamycin (autophagy activation) or chloroquine (autophagy inhibition). B This panel shows densitometric quantification of LC3-II and p62 normalized to GAPDH, respectively, presented as mean ± SD from independent experiments performed separately in each cell line. P ***-value < 0.001

    Journal: Journal of Molecular Histology

    Article Title: Autophagy hub genes mediate photodynamic therapy tolerance in nasopharyngeal carcinoma through cytoprotective autophagy and survival signaling

    doi: 10.1007/s10735-026-10746-x

    Figure Lengend Snippet: PDT induces autophagic flux in nasopharyngeal carcinoma cells. A This panel shows representative western blots of LC3-II, p62/SQSTM1, and GAPDH in C666-1 and HONE1 cells, respectively, treated with PDT alone or in combination with rapamycin (autophagy activation) or chloroquine (autophagy inhibition). B This panel shows densitometric quantification of LC3-II and p62 normalized to GAPDH, respectively, presented as mean ± SD from independent experiments performed separately in each cell line. P ***-value < 0.001

    Article Snippet: Membranes were blocked in 5% non-fat milk in TBST and incubated overnight at 4 °C with primary antibodies against AKT1 (CST #2938, 1:1000), BECN1/Beclin-1 (CST #3738, 1:1000), BCL2 (CST #2876, 1:1000), ATG5 (CST #2630, 1:1000), LC3B (CST #3868, 1:1000), p62/SQSTM1 (CST #5114, 1:1000), and the loading control GAPDH (CST #2118, 1:5000).

    Techniques: Western Blot, Activation Assay, Inhibition