fresh medium (Beijing Solarbio Science)
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Fresh Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 10930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 10930 article reviews
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Incubation:Article Title: Magnetic Resonance Imaging Reveals Meningeal Lymphatic Impairment in Lung Adenocarcinoma Brain Metastasis Progression Article Snippet: .. After 24‐h incubation, we aspirated supernatant, added 90 μL Article Title: RRM2-mediated inhibition of GPX4 ubiquitination: A novel mechanism for ferroptosis suppression and reduced cisplatin sensitivity in small cell lung cancer. Article Snippet: RRM2 is an important regulatory protein in ferroptosis pathways, but its role in small cell lung cancer (SCLC), which is characterized by rapid proliferation, frequent metastasis, and drug resistance, remains unclear.. Here, we report that RRM2 is highly expressed in SCLC tissues and has excellent diagnostic potential.. In vitro, our experiments demonstrate that knockdown of RRM2 expression significantly inhibited the proliferation, migration, and invasiveness of NCI-H446 and SBC-2 cells, while overexpression had the opposite effect.Furthermore, RRM2 inhibition significantly enhanced the activity of the ferroptosis pathway in SCLC cells. MTT Assay:Article Title: Magnetic Resonance Imaging Reveals Meningeal Lymphatic Impairment in Lung Adenocarcinoma Brain Metastasis Progression Article Snippet: .. After 24‐h incubation, we aspirated supernatant, added 90 μL CCK-8 Assay:Article Title: SDF2 promotes glioma progression via GRP78-mediated ERAD and copper homeostasis disruption Article Snippet: .. Article Title: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis. Article Snippet: .. After cultured overnight, Article Title: LITAF suppresses breast cancer and paclitaxel resistance by ubiquitinating and degrading PCMT1 to inhibit COX-2-dependent arachidonic acid metabolism Article Snippet: Prostaglandin E2 (PGE2) and AA levels were measured using the PGE2 ELISA Kit (ab316263) and AA ELISA Kit (ab287798) from Abcam, respectively, following the manufacturer’s instructions. .. For cell viability, the culture supernatant was removed from each well, followed by the addition of Cell Counting Kit-8 (CCK-8, 10 μL, Solarbio) and Article Title: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis Article Snippet: .. After cultured overnight, Infection:Article Title: Hypoxia-driven remodeling of SELENOP + macrophages shapes T cell dynamics and promotes ovarian cancer metastasis Article Snippet: For the generation of SELENOP -overexpressing or EPHB2 -deficient THP-1 cell lines, THP-1 cells were infected by lentivirus at a multiplicity of infection (MOI) of 60, with 10 μg/mL polybrene (Cat#HB-PB-500, Hanbio) added to enhance transduction efficiency. .. Selection:Article Title: Hypoxia-driven remodeling of SELENOP + macrophages shapes T cell dynamics and promotes ovarian cancer metastasis Article Snippet: For the generation of SELENOP -overexpressing or EPHB2 -deficient THP-1 cell lines, THP-1 cells were infected by lentivirus at a multiplicity of infection (MOI) of 60, with 10 μg/mL polybrene (Cat#HB-PB-500, Hanbio) added to enhance transduction efficiency. .. Transfection:Article Title: RRM2-mediated inhibition of GPX4 ubiquitination: A novel mechanism for ferroptosis suppression and reduced cisplatin sensitivity in small cell lung cancer. Article Snippet: RRM2 is an important regulatory protein in ferroptosis pathways, but its role in small cell lung cancer (SCLC), which is characterized by rapid proliferation, frequent metastasis, and drug resistance, remains unclear.. Here, we report that RRM2 is highly expressed in SCLC tissues and has excellent diagnostic potential.. In vitro, our experiments demonstrate that knockdown of RRM2 expression significantly inhibited the proliferation, migration, and invasiveness of NCI-H446 and SBC-2 cells, while overexpression had the opposite effect.Furthermore, RRM2 inhibition significantly enhanced the activity of the ferroptosis pathway in SCLC cells. Concentration Assay:Article Title: RRM2-mediated inhibition of GPX4 ubiquitination: A novel mechanism for ferroptosis suppression and reduced cisplatin sensitivity in small cell lung cancer. Article Snippet: RRM2 is an important regulatory protein in ferroptosis pathways, but its role in small cell lung cancer (SCLC), which is characterized by rapid proliferation, frequent metastasis, and drug resistance, remains unclear.. Here, we report that RRM2 is highly expressed in SCLC tissues and has excellent diagnostic potential.. In vitro, our experiments demonstrate that knockdown of RRM2 expression significantly inhibited the proliferation, migration, and invasiveness of NCI-H446 and SBC-2 cells, while overexpression had the opposite effect.Furthermore, RRM2 inhibition significantly enhanced the activity of the ferroptosis pathway in SCLC cells. Cell Culture:Article Title: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis. Article Snippet: .. After cultured overnight, Article Title: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis Article Snippet: .. After cultured overnight, |
