Review



fresh medium  (Beijing Solarbio Science)


Bioz Verified Symbol Beijing Solarbio Science is a verified supplier
Bioz Manufacturer Symbol Beijing Solarbio Science manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Beijing Solarbio Science fresh medium
    Fresh Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 10930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+medium/Medium/pmc13111324-82-24-22
    Average 99 stars, based on 10930 article reviews
    fresh medium - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Magnetic Resonance Imaging Reveals Meningeal Lymphatic Impairment in Lung Adenocarcinoma Brain Metastasis Progression
    Article Snippet: .. After 24‐h incubation, we aspirated supernatant, added 90 μL fresh medium and 10 μL MTT solution (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; M1020, Solarbio), and incubated cells for 2 h. Following supernatant removal, we added 110 μL dimethyl sulfoxide (DMSO) per well. ..

    Article Title: RRM2-mediated inhibition of GPX4 ubiquitination: A novel mechanism for ferroptosis suppression and reduced cisplatin sensitivity in small cell lung cancer.
    Article Snippet: RRM2 is an important regulatory protein in ferroptosis pathways, but its role in small cell lung cancer (SCLC), which is characterized by rapid proliferation, frequent metastasis, and drug resistance, remains unclear.. Here, we report that RRM2 is highly expressed in SCLC tissues and has excellent diagnostic potential.. In vitro, our experiments demonstrate that knockdown of RRM2 expression significantly inhibited the proliferation, migration, and invasiveness of NCI-H446 and SBC-2 cells, while overexpression had the opposite effect.Furthermore, RRM2 inhibition significantly enhanced the activity of the ferroptosis pathway in SCLC cells.

    MTT Assay:

    Article Title: Magnetic Resonance Imaging Reveals Meningeal Lymphatic Impairment in Lung Adenocarcinoma Brain Metastasis Progression
    Article Snippet: .. After 24‐h incubation, we aspirated supernatant, added 90 μL fresh medium and 10 μL MTT solution (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; M1020, Solarbio), and incubated cells for 2 h. Following supernatant removal, we added 110 μL dimethyl sulfoxide (DMSO) per well. ..

    CCK-8 Assay:

    Article Title: SDF2 promotes glioma progression via GRP78-mediated ERAD and copper homeostasis disruption
    Article Snippet: .. Fresh medium (100 μ l) was added to each well, followed by 10 μ l of CCK-8 solution (Beijing Solarbio Science & Technology Co., Ltd.). ..

    Article Title: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis.
    Article Snippet: .. After cultured overnight, fresh medium containing 10 μl of CCK-8 solution (Solarbio, Beijing, China) was added for 1 h at the 37 °C incubator and the absorbance of each well at the wavelength at 450 nm was detected. ..

    Article Title: LITAF suppresses breast cancer and paclitaxel resistance by ubiquitinating and degrading PCMT1 to inhibit COX-2-dependent arachidonic acid metabolism
    Article Snippet: Prostaglandin E2 (PGE2) and AA levels were measured using the PGE2 ELISA Kit (ab316263) and AA ELISA Kit (ab287798) from Abcam, respectively, following the manufacturer’s instructions. .. For cell viability, the culture supernatant was removed from each well, followed by the addition of Cell Counting Kit-8 (CCK-8, 10 μL, Solarbio) and fresh medium (90 μL) per well. .. After 60 min of incubation, the optical density at 450 nm (OD450) was measured using a microplate reader (Molecular Devices, USA).

    Article Title: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis
    Article Snippet: .. After cultured overnight, fresh medium containing 10 μl of CCK-8 solution (Solarbio, Beijing, China) was added for 1 h at the 37 °C incubator and the absorbance of each well at the wavelength at 450 nm was detected. ..

    Infection:

    Article Title: Hypoxia-driven remodeling of SELENOP + macrophages shapes T cell dynamics and promotes ovarian cancer metastasis
    Article Snippet: For the generation of SELENOP -overexpressing or EPHB2 -deficient THP-1 cell lines, THP-1 cells were infected by lentivirus at a multiplicity of infection (MOI) of 60, with 10 μg/mL polybrene (Cat#HB-PB-500, Hanbio) added to enhance transduction efficiency. .. Fresh medium was changed 48 h post infection, supplemented with 1 mg/mL puromycin (Cat# P8230, Solarbio) for selection. ..

    Selection:

    Article Title: Hypoxia-driven remodeling of SELENOP + macrophages shapes T cell dynamics and promotes ovarian cancer metastasis
    Article Snippet: For the generation of SELENOP -overexpressing or EPHB2 -deficient THP-1 cell lines, THP-1 cells were infected by lentivirus at a multiplicity of infection (MOI) of 60, with 10 μg/mL polybrene (Cat#HB-PB-500, Hanbio) added to enhance transduction efficiency. .. Fresh medium was changed 48 h post infection, supplemented with 1 mg/mL puromycin (Cat# P8230, Solarbio) for selection. ..

    Transfection:

    Article Title: RRM2-mediated inhibition of GPX4 ubiquitination: A novel mechanism for ferroptosis suppression and reduced cisplatin sensitivity in small cell lung cancer.
    Article Snippet: RRM2 is an important regulatory protein in ferroptosis pathways, but its role in small cell lung cancer (SCLC), which is characterized by rapid proliferation, frequent metastasis, and drug resistance, remains unclear.. Here, we report that RRM2 is highly expressed in SCLC tissues and has excellent diagnostic potential.. In vitro, our experiments demonstrate that knockdown of RRM2 expression significantly inhibited the proliferation, migration, and invasiveness of NCI-H446 and SBC-2 cells, while overexpression had the opposite effect.Furthermore, RRM2 inhibition significantly enhanced the activity of the ferroptosis pathway in SCLC cells.

    Concentration Assay:

    Article Title: RRM2-mediated inhibition of GPX4 ubiquitination: A novel mechanism for ferroptosis suppression and reduced cisplatin sensitivity in small cell lung cancer.
    Article Snippet: RRM2 is an important regulatory protein in ferroptosis pathways, but its role in small cell lung cancer (SCLC), which is characterized by rapid proliferation, frequent metastasis, and drug resistance, remains unclear.. Here, we report that RRM2 is highly expressed in SCLC tissues and has excellent diagnostic potential.. In vitro, our experiments demonstrate that knockdown of RRM2 expression significantly inhibited the proliferation, migration, and invasiveness of NCI-H446 and SBC-2 cells, while overexpression had the opposite effect.Furthermore, RRM2 inhibition significantly enhanced the activity of the ferroptosis pathway in SCLC cells.

    Cell Culture:

    Article Title: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis.
    Article Snippet: .. After cultured overnight, fresh medium containing 10 μl of CCK-8 solution (Solarbio, Beijing, China) was added for 1 h at the 37 °C incubator and the absorbance of each well at the wavelength at 450 nm was detected. ..

    Article Title: LINC02159 modulated the glycolysis and proliferation of TNBC cell via targeting miR-1285-3p/G6PI axis
    Article Snippet: .. After cultured overnight, fresh medium containing 10 μl of CCK-8 solution (Solarbio, Beijing, China) was added for 1 h at the 37 °C incubator and the absorbance of each well at the wavelength at 450 nm was detected. ..



    Similar Products

    99
    MedChemExpress fresh complete medium
    Fresh Complete Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+medium/Puromycin/10__1016_slash_j__apsb__2026__06__031-195-13-20
    Average 99 stars, based on 1 article reviews
    fresh complete medium - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Beijing Solarbio Science fresh medium
    Fresh Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+medium/Medium/pmc13111324-82-24-22
    Average 99 stars, based on 1 article reviews
    fresh medium - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    95
    Valiant Co Ltd fresh lb medium with vancomycin
    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of <t>vancomycin</t> in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.
    Fresh Lb Medium With Vancomycin, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+medium/LB+medium/pmc13010146-103-11-16
    Average 95 stars, based on 1 article reviews
    fresh lb medium with vancomycin - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    86
    Hyponex Corporation fresh hyponex medium
    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of <t>vancomycin</t> in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.
    Fresh Hyponex Medium, supplied by Hyponex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+medium/fresh+hyponex+medium/pmc13169837-44-10-11
    Average 86 stars, based on 1 article reviews
    fresh hyponex medium - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    MedChemExpress fresh differentiation medium
    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of <t>vancomycin</t> in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.
    Fresh Differentiation Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+medium/SC79/pm41899473-121-19-26
    Average 99 stars, based on 1 article reviews
    fresh differentiation medium - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    94
    Elabscience Biotechnology fresh complete medium
    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of <t>vancomycin</t> in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.
    Fresh Complete Medium, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fresh+medium/MEM%C3%97%C3%97+Complete+Medium/pmc12813741-56-12-24
    Average 94 stars, based on 1 article reviews
    fresh complete medium - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of vancomycin in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.

    Journal: Nucleic Acids Research

    Article Title: A WYL transcriptional regulator activates the DNA damage response pathway in Acinetobacter species

    doi: 10.1093/nar/gkag250

    Figure Lengend Snippet: A C-terminal 3xFLAG tag constitutively activates AciT. ( A ) Representative microscopy images of indicated strains. Scale bars, 10 µm. ( B ) Representative western blot on indicated strains grown with or without 2 µg/ml MMC. Blot was probed with ⍺-RpoA antibody as a loading control and ⍺-FLAG antibody to probe for AciT expression. ( C ) Growth curves of indicated strains grown with indicated concentrations of vancomycin in 96-well plates over 12 h. Arrows indicate concentration used for selection experiments. Each dot shows the mean of three independent, biological replicates, and error bars show SEM.

    Article Snippet: Then, 1 μl of overnight cultures were subcultured into 200 μl fresh LB medium with vancomycin (MP Biomedicals) (0 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 250 μg/ml) grown in a 96-well plate in a Synergy H1 multimode plate reader (BioTek) utilizing Gen 6 software at 30°C shaking for 16 h. OD 600 was measured every 10 min.

    Techniques: Microscopy, Western Blot, Control, Expressing, Concentration Assay, Selection