fopflash (Addgene inc)
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Addgene inc
fopflash
Fopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fopflash/M51+Super+8x+FOPFlash+(TOPFlash+mutant)+(Plasmid+%2312457)/pm41906239-113-16-17
Average 94 stars, based on 257 article reviews
Fopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fopflash/M51+Super+8x+FOPFlash+(TOPFlash+mutant)+(Plasmid+%2312457)/pm41906239-113-16-17
Average 94 stars, based on 257 article reviews
fopflash - by Bioz Stars,
2026-09
94/100 stars
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Transfection:Article Title: NR3C1 Modulates Wnt Signalling to Influence the Invasiveness and Immune Features of Nonfunctioning Invasive Pituitary Adenomas. Article Snippet: .. Cells were co- transfected using Lipofectamine 3000 (Thermo Fisher, L3000015) with either TOPflash (Addgene, #12456) or Article Title: The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathway Article Snippet: The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure the activities of firefly luciferase and renilla luciferase in the cell lysates collected 24 h post-transfection, according to the manufacturer’s instructions. .. For the Wnt/β-catenin reporter assay in primary oviductal epithelial cells, primary oviductal epithelial cells were transfected with TOPflash (Addgene, catalog no. 12456, USA) or Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics Article Snippet: Transient transfections were performed at 60% confluence in 96-well plates (Corning Falcon Microplate, Cat. No. CLS353296). .. Cells were transfected using Lipofectamine 3000 (Invitrogen, Cat. No. L3000-008) with plasmids containing the wild type or variant DVL genes plus firefly reporter plasmids for SuperTopFlash (M50 Super 8x TOPFlash, Addgene plasmid #12456) and Article Title: Endometrial stromal Menin supports endometrial receptivity by maintaining homeostasis of WNT signaling pathway through H3K4me3 during WOI. Article Snippet: Gene Set Enrichment Analysis (GSEA) was conducted on the GSEA analysis tool (http://www. broadinstitute.org/gsea/index.jsp). .. Primary hESCs were seeded onto 24-well plates at the density of approximately 60% and transfected with either TOPflash (#12456, Addgene) or Article Title: The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathway. Article Snippet: The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure the activities of firefly luciferase and renilla luciferase in the cell lysates collected 24 h post-transfection, according to the manufacturer’s instructions. .. For the Wnt/β-catenin reporter assay in primary oviductal epithelial cells, primary oviductal epithelial cells were transfected with TOPflash (Addgene, catalog no. 12456, USA) or Control:Article Title: NR3C1 Modulates Wnt Signalling to Influence the Invasiveness and Immune Features of Nonfunctioning Invasive Pituitary Adenomas. Article Snippet: .. Cells were co- transfected using Lipofectamine 3000 (Thermo Fisher, L3000015) with either TOPflash (Addgene, #12456) or Article Title: Endometrial stromal Menin supports endometrial receptivity by maintaining homeostasis of WNT signaling pathway through H3K4me3 during WOI. Article Snippet: Gene Set Enrichment Analysis (GSEA) was conducted on the GSEA analysis tool (http://www. broadinstitute.org/gsea/index.jsp). .. Primary hESCs were seeded onto 24-well plates at the density of approximately 60% and transfected with either TOPflash (#12456, Addgene) or Reporter Assay:Article Title: The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathway Article Snippet: The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure the activities of firefly luciferase and renilla luciferase in the cell lysates collected 24 h post-transfection, according to the manufacturer’s instructions. .. For the Wnt/β-catenin reporter assay in primary oviductal epithelial cells, primary oviductal epithelial cells were transfected with TOPflash (Addgene, catalog no. 12456, USA) or Article Title: The miR-34/449 clusters regulate the differentiation of ciliated cells in the oviduct via the Wnt/β-catenin signaling pathway. Article Snippet: The Dual-Luciferase Reporter Assay System (Promega, catalog no. E1910, USA) was used to measure the activities of firefly luciferase and renilla luciferase in the cell lysates collected 24 h post-transfection, according to the manufacturer’s instructions. .. For the Wnt/β-catenin reporter assay in primary oviductal epithelial cells, primary oviductal epithelial cells were transfected with TOPflash (Addgene, catalog no. 12456, USA) or Variant Assay:Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics Article Snippet: Transient transfections were performed at 60% confluence in 96-well plates (Corning Falcon Microplate, Cat. No. CLS353296). .. Cells were transfected using Lipofectamine 3000 (Invitrogen, Cat. No. L3000-008) with plasmids containing the wild type or variant DVL genes plus firefly reporter plasmids for SuperTopFlash (M50 Super 8x TOPFlash, Addgene plasmid #12456) and Plasmid Preparation:Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics Article Snippet: Transient transfections were performed at 60% confluence in 96-well plates (Corning Falcon Microplate, Cat. No. CLS353296). .. Cells were transfected using Lipofectamine 3000 (Invitrogen, Cat. No. L3000-008) with plasmids containing the wild type or variant DVL genes plus firefly reporter plasmids for SuperTopFlash (M50 Super 8x TOPFlash, Addgene plasmid #12456) and Article Title: Phosphoglycerate kinase 1 stimulates β-catenin signaling, which is further enhanced by the transfection of bovine alphaherpesvirus 1 latency-related protein ORF2 Article Snippet: Bovine alphaherpesvirus 1 (BoAHV-1) mainly infects cattle and causes substantial economic losses to the cattle industry worldwide.. The virus establishes a lifelong latent infection primarily in the trigeminal ganglia (TG), where the open reading frame 2 (ORF2), a product of viral latency-related (LR) gene is abundantly expressed.. ORF2 has anti-apoptotic effects, in part by upregulating β-catenin signaling. Negative Control:Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics Article Snippet: Transient transfections were performed at 60% confluence in 96-well plates (Corning Falcon Microplate, Cat. No. CLS353296). .. Cells were transfected using Lipofectamine 3000 (Invitrogen, Cat. No. L3000-008) with plasmids containing the wild type or variant DVL genes plus firefly reporter plasmids for SuperTopFlash (M50 Super 8x TOPFlash, Addgene plasmid #12456) and Luciferase:Article Title: Pathogenic DVL frameshifting variants in Robinow syndrome disrupt WNT signaling and cellular dynamics Article Snippet: Transient transfections were performed at 60% confluence in 96-well plates (Corning Falcon Microplate, Cat. No. CLS353296). .. Cells were transfected using Lipofectamine 3000 (Invitrogen, Cat. No. L3000-008) with plasmids containing the wild type or variant DVL genes plus firefly reporter plasmids for SuperTopFlash (M50 Super 8x TOPFlash, Addgene plasmid #12456) and |
