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fncas12a  (Nature Biotechnology)

 
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    Structured Review

    Nature Biotechnology fncas12a
    Fncas12a, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fncas12a/fncas12a/pm38811761-576-36-0
    Average 90 stars, based on 1 article reviews
    fncas12a - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Trans-nuclease activity of Cas9 activated by DNA or RNA target binding.
    Article Snippet: Type V and type VI CRISPR–Cas systems have been shown to cleave nonspecific single-stranded DNA (ssDNA) or single-stranded RNA (ssRNA) in trans, but this has not been observed in type II CRISPR–Cas systems using single guide RNA.. We show here that the type II CRISPR–Cas9 systems directed by CRISPR RNA and trans-activating CRISPR RNA dual RNAs show RuvC domain-dependent trans-cleavage activity for both ssDNA and ssRNA substrates.. Cas9 possesses sequence preferences for trans-cleavage substrates, preferring to cleave Tor C-rich ssDNA substrates.



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    a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG <t>(FnCas12a</t> variants); paired t -test, two tailed. Source data are provided as a Source Data file.
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    a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG (FnCas12a variants); paired t -test, two tailed. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Highly parallel profiling of the activities and specificities of Cas12a variants in human cells

    doi: 10.1038/s41467-025-57150-9

    Figure Lengend Snippet: a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG (FnCas12a variants); paired t -test, two tailed. Source data are provided as a Source Data file.

    Article Snippet: AsCas12a (Addgene, #69982), LbCas12a (Addgene, #69988), FnCas12a (Addgene, #69976), Lb2Cas12a (Addgene, #69983) human expression plasmids were purchased from the non-profit plasmid repository Addgene.

    Techniques: Variant Assay, Transduction, Labeling, Comparison, Two Tailed Test