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Merck & Co fluoroshield mounting medium with dapi
Fluoroshield Mounting Medium With Dapi, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluoroshield/entellan/pm41871906-118-14-19
Average 86 stars, based on 1 article reviews
fluoroshield mounting medium with dapi - by Bioz Stars, 2026-09
86/100 stars

Images

Related Articles

Microscopy:

Article Title: Calcineurin inhibition mitigates activation of enteric glia in vitro and affects their crosstalk with macrophages.
Article Snippet: After washing, cells were incubated with Alexa Fluor (AF)-546conjugated anti-rabbit secondary antibody (1:300; Cat. No. A10040; Thermofisher) or AF-488 Plus-conjugated anti-mouse (1:300, Cat. No. A32790; Thermofisher), diluted in InsidePerm solution, for 45 min at RT. .. Slides were mounted with 4′,6-diamidino-2-phenylindole (DAPI)containing Fluoroshield (Merck) and digital images were captured with a Zeiss Observer.Z1 microscope (Zeiss, Oberkochen, Germany) at 20X magnification. ..

Article Title: Hypoxia reverses the early anti-inflammatory microglial response to the β-amyloid peptide: mitochondrial involvement and beneficial roles of melatonin and naringenin.
Article Snippet: After washing, cells were incubated with AlexaFluor 546-conjugated secondary antibody (1:300; Thermofisher), diluted in InsidePerm solution, for 45 min at RT. .. Slides were mounted with Fluoroshield (Merck) and digital images were captured with a Zeiss Observer.Z1 microscope (Zeiss, Oberkochen, Germany). ..

Article Title: Hypoxia reverses the early anti-inflammatory microglial response to the β-amyloid peptide: mitochondrial involvement and beneficial roles of melatonin and naringenin
Article Snippet: After washing, cells were incubated with AlexaFluor 546-conjugated secondary antibody (1:300; Thermofisher), diluted in InsidePerm solution, for 45 min at RT. .. Slides were mounted with Fluoroshield (Merck) and digital images were captured with a Zeiss Observer.Z1 microscope (Zeiss, Oberkochen, Germany). ..

Staining:

Article Title: Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair
Article Snippet: The primary antibodies (human beta 2-microglobulin; Thermofisher, PA5142897, 1:100 (v/v); H3K14ac (Abcam, ab52946, 1:100 v/v); H3K9ac (Abcam, ab10812, 1:100 v/v); H3K27ac (Abcam, ab4729, 1:100 v/v) were diluted in a staining buffer (0.05% Triton X-100, 1% BSA in PBS) and left overnight at +4°C in a humid atmosphere. .. The samples were then washed with 0.1% Triton X-100 in PBS (3 × 5 min), then the secondary antibodies (Donkey anti-goat - AF488; Thermofisher, A-11055; 1:1000 v/v; Donkey anti-rabbit-AF555; Thermofisher, A-31572; 1:1000 v/v) were added to the sample in the staining buffer containing DAPI (1:1000 v/v) for 2 h at room temperature in the dark.After incubation with the secondary antibodies, the samples were washed with 0.1% Triton X-100 in PBS (3 × 5 min), and mounted with Fluoroshield (Merck, F6182). ..

Article Title: Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair.
Article Snippet: The primary antibodies (human beta 2-microglobulin; Thermofisher, PA5142897, 1:100 (v/v); H3K14ac (Abcam, ab52946, 1:100 v/v); H3K9ac (Abcam, ab10812, 1:100 v/v); H3K27ac (Abcam, ab4729, 1:100 v/v) were diluted in a staining buffer (0.05% Triton X-100, 1% BSA in PBS) and left overnight at +4°C in a humid atmosphere. .. The samples were then washed with 0.1% Triton X-100 in PBS (3 × 5 min), then the secondary antibodies (Donkey anti-goat -AF488; Thermofisher, A-11055; 1:1000 v/v; Donkey anti-rabbit-AF555; Thermofisher, A-31572; 1:1000 v/v) were added to the sample in the staining buffer containing DAPI (1:1000 v/v) for 2 h at room temperature in the dark.After incubation with the secondary antibodies, the samples were washed with 0.1% Triton X-100 in PBS (3 × 5 min), and mounted with Fluoroshield (Merck, F6182). ..

Incubation:

Article Title: Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair
Article Snippet: The primary antibodies (human beta 2-microglobulin; Thermofisher, PA5142897, 1:100 (v/v); H3K14ac (Abcam, ab52946, 1:100 v/v); H3K9ac (Abcam, ab10812, 1:100 v/v); H3K27ac (Abcam, ab4729, 1:100 v/v) were diluted in a staining buffer (0.05% Triton X-100, 1% BSA in PBS) and left overnight at +4°C in a humid atmosphere. .. The samples were then washed with 0.1% Triton X-100 in PBS (3 × 5 min), then the secondary antibodies (Donkey anti-goat - AF488; Thermofisher, A-11055; 1:1000 v/v; Donkey anti-rabbit-AF555; Thermofisher, A-31572; 1:1000 v/v) were added to the sample in the staining buffer containing DAPI (1:1000 v/v) for 2 h at room temperature in the dark.After incubation with the secondary antibodies, the samples were washed with 0.1% Triton X-100 in PBS (3 × 5 min), and mounted with Fluoroshield (Merck, F6182). ..

Article Title: Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair.
Article Snippet: The primary antibodies (human beta 2-microglobulin; Thermofisher, PA5142897, 1:100 (v/v); H3K14ac (Abcam, ab52946, 1:100 v/v); H3K9ac (Abcam, ab10812, 1:100 v/v); H3K27ac (Abcam, ab4729, 1:100 v/v) were diluted in a staining buffer (0.05% Triton X-100, 1% BSA in PBS) and left overnight at +4°C in a humid atmosphere. .. The samples were then washed with 0.1% Triton X-100 in PBS (3 × 5 min), then the secondary antibodies (Donkey anti-goat -AF488; Thermofisher, A-11055; 1:1000 v/v; Donkey anti-rabbit-AF555; Thermofisher, A-31572; 1:1000 v/v) were added to the sample in the staining buffer containing DAPI (1:1000 v/v) for 2 h at room temperature in the dark.After incubation with the secondary antibodies, the samples were washed with 0.1% Triton X-100 in PBS (3 × 5 min), and mounted with Fluoroshield (Merck, F6182). ..



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