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fluoroshield mounting medium with dapi  (Beyotime)


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    Structured Review

    Beyotime fluoroshield mounting medium with dapi
    Fluoroshield Mounting Medium With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshield/DAPI/pmc13124985-211-29-34
    Average 99 stars, based on 32249 article reviews
    fluoroshield mounting medium with dapi - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Confocal Microscopy:

    Article Title: Engineered bacteriophage nanoassemblies in vivo stabilize DC-T cell immune synapse for high-performance influenza vaccination.
    Article Snippet: T cell activation by dendritic cells (DCs) requires the formation of a stable immune synapse (IS).. The objective of this study was to develop a vaccination approach by targetedly regulating DC–T cell synaptic interactions in vivo.. We constructed bacteriophage nanoassemblies to deliver antigen-encoding sequences into DC cytoplasm and enhance DC–T cell IS stability in vivo, thereby boosting vaccine potency.

    Incubation:

    Article Title: Red yeast rice extract’s impact on liver health: a pharmacological and metabolomic exploration
    Article Snippet: .. Then, the sections were incubated with anti-rabbit antibody labeled with secondary antibodies (Goat anti-rabbit IgG H&L(HRP), 1:20000, abcam, Cat. Ab205718 ) for 1 h. The nuclei were visualized by Fluoroshield mounting medium with DAPI (Beyotime Biotechnology, Shanghai, China) staining. .. Immunohistochemistry analysis were performed using Image ProPlus 6.0 software (Media Cybernetics Inc., Silver Spring, MD, United States) for semiquantitative analysis.

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.

    Labeling:

    Article Title: Red yeast rice extract’s impact on liver health: a pharmacological and metabolomic exploration
    Article Snippet: .. Then, the sections were incubated with anti-rabbit antibody labeled with secondary antibodies (Goat anti-rabbit IgG H&L(HRP), 1:20000, abcam, Cat. Ab205718 ) for 1 h. The nuclei were visualized by Fluoroshield mounting medium with DAPI (Beyotime Biotechnology, Shanghai, China) staining. .. Immunohistochemistry analysis were performed using Image ProPlus 6.0 software (Media Cybernetics Inc., Silver Spring, MD, United States) for semiquantitative analysis.

    Staining:

    Article Title: Red yeast rice extract’s impact on liver health: a pharmacological and metabolomic exploration
    Article Snippet: .. Then, the sections were incubated with anti-rabbit antibody labeled with secondary antibodies (Goat anti-rabbit IgG H&L(HRP), 1:20000, abcam, Cat. Ab205718 ) for 1 h. The nuclei were visualized by Fluoroshield mounting medium with DAPI (Beyotime Biotechnology, Shanghai, China) staining. .. Immunohistochemistry analysis were performed using Image ProPlus 6.0 software (Media Cybernetics Inc., Silver Spring, MD, United States) for semiquantitative analysis.

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.

    Blocking Assay:

    Article Title: Histamine H 1 receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice
    Article Snippet: .. After blocking by 5% normal donkey serum in PBS for 1 h at room temperature, sections were incubated with anti-ChAT antibody (Millipore, AB144P, 1:100), anti-c-Fos antibody (Synaptic System, 226004, 1:500), at 4 °C overnight and then with AlexaFluor-conjugated secondary antibody-Donkey anti-Goat Alexa Fluor 488 (Invitrogen, A32814, 1:400) or Goat Anti-Guinea pig Alexa Fluor 647 (Abcam, ab150187, 1:400) at room temperature for 2 h. Fluoroshield TM with DAPI (Beyotime, P0131) was used as a nuclear stain. .. The slices were photographed by Olympus FV3000 confocal microscope and further quantified by FIJI (ImageJ-win64) in a blind manner.



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