dapi (SouthernBiotech)

SouthernBiotech is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

SouthernBiotech dapi

ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were <t>counterstained</t> with <t>DAPI</t> (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

Dapi, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 4740 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluoromount+g/DAPI+Fluoromount-G/pmc13677000-423-6-9
Average 96 stars, based on 4740 article reviews

dapi - by Bioz Stars, 2026-10

96/100 stars

Images

1) Product Images from "Astrocyte‐Associated ABCA1/APOE Responses Accompany Hippocampal Lipid Remodeling and Ectopic Neuronal Accumulation During Epileptogenesis"

Article Title: Astrocyte‐Associated ABCA1/APOE Responses Accompany Hippocampal Lipid Remodeling and Ectopic Neuronal Accumulation During Epileptogenesis

Journal: Advanced Science

doi: 10.1002/advs.78329

Figure Legend Snippet: ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were counterstained with DAPI (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

Techniques Used: Expressing, Western Blot, Analysis, Immunofluorescence, Marker, Multiplex Assay

Fluorescence:

Article Title: NRF2 activation attenuates obesity-induced adipose inflammation by suppressing STING signaling, with depot-specific regulation.
Article Snippet: .. Samples were mounted with Fluoromount-G (SouthernBiotech, Cat. No. 0100-01) and imaged using a fluorescence microscope (DMi8, Leica) or a laser confocal microscope (C2, Nikon). .. Colocalization was quantified using ImageJ software (v1.53c) by calculating Pearson’s correlation coefficient.

Microscopy:

Article Title: Human trilayer engineered blood vessel reveals influence of fibroblasts on disease progression in model of Hutchinson-Gilford progeria.
Article Snippet: .. After washing, sections were mounted with a coverslip using Fluoromount- G (Southern Biotech) and imaged using either a Nikon ECLIPSE Ti inverted microscope or Leica Stellaris 8 confocal microscope. .. Some cross sections were used to evaluate cell death using the Apoptosis/Necrosis Assay Kit (Abcam, 176749) per the manufacturer’s instructions.

Confocal:

Inverted Microscopy:

Glass:

Article Title: Thymic stromal defects and immune dysregulation in hypomorphic Rag deficiency: implications for hematopoietic cell transplantation.
Article Snippet: .. Samples were washed 122 3× in PBS (10 min per wash), mounted using Fluoromount G (Southern Biotech), covered with a 123 No. 1.5 cover glass (VWR, Cat#: 48393-241), and allowed to cure (8–24 h). .. Images were acquired 124 using an inverted Leica Stellaris equipped with a pulsed white-light laser and four tunable spectral 125 hybrid detectors.

Incubation:

Article Title: Attenuation of Cardiac Dysfunction in the Collagen-Induced Arthritis Mouse Model Mediated by a Reactive Aldehyde Species Inhibitor.
Article Snippet: Detection of anti-CIT antibody was carried out using AF488 AffiniPure Donkey anti-mouse IgM (1:1000; Jackson ImmunoResearch, West Grove, PA, USA). .. After a 45 min incubation with secondary antibody at room temperature, 4′,6-diamidino-2-phenylindole (DAPI) was applied, and samples were mounted with Fluoromount-G (Southern Biotech, Birmingham, https://doi.org/10.3390/ijms27198478 AL, USA). ..

Review