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fluorogenic hdac4 assay kit  (BPS Bioscience)


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    Structured Review

    BPS Bioscience fluorogenic hdac4 assay kit
    Fluorogenic Hdac4 Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorogenic+hdac4+assay+kit/HDAC4+Fluorogenic+Assay+Kit/10__4103_slash_apjtb__apjtb_622_25-93-6-10
    Average 94 stars, based on 24 article reviews
    fluorogenic hdac4 assay kit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Enzymatic Assay:

    Article Title: Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue
    Article Snippet: .. Fluorogenic Enzymatic Assay for HDAC4 HDAC4 inhibitory activity was determined using the fluorogenic HDAC4 assay kit (BPS Bioscience, catalogue # 50064) according to the manufacturer procedure. .. The statistical data analysis and IC 50 values were determined using GraphPad Prism 7.02.

    Activity Assay:

    Article Title: Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue
    Article Snippet: .. Fluorogenic Enzymatic Assay for HDAC4 HDAC4 inhibitory activity was determined using the fluorogenic HDAC4 assay kit (BPS Bioscience, catalogue # 50064) according to the manufacturer procedure. .. The statistical data analysis and IC 50 values were determined using GraphPad Prism 7.02.

    Article Title: Utilization of cyanopyridine in design and synthesis of first-in-class anticancer dual acting PIM-1 kinase/HDAC inhibitors.
    Article Snippet: Herein, we report design and synthesis of twenty-one dual PIM-1/HDAC inhibitors utilizing 3-cyanopyridines as a novel cap moiety linked with aliphatic /aromatic linker bearing carboxylic acid 3a-g, hydroxamic acid 4a-g or 2-aminoanilide moieties 5a-g as zinc-binding group.. Most of the target hybrids revealed promising growth inhibition according to one dose NCI protocol against 60 cancer cell lines.. Meanwhile, hydroxamic acids 4b, 4d and 4e displayed strong and broad-spectrum activity against nine tumor subpanels tested (GI50 0.176–8.87 μM); 4d displayed strong antiproliferative activity with GI50 ≤ 3 μM against different cancer cell lines (GI50 range from 0.325 to 2.9 μM).

    Article Title: Curcumin attenuates pressure overload-induced cardiac fibrosis in mice via modulating the miR-29b/HDAC4 axis
    Article Snippet: Protein bands were visualized using ECL reagents (Thermo Fisher, USA, 34580), and densitometric analysis was performed with ImageJ. .. HDAC4 activity was measured using the Fluorogenic HDAC4 Assay Kit (BPS Bioscience, #50064) following the manufacturer’s protocol. .. Tissue lysates were prepared from left ventricular tissue using the lysis buffer provided in the Fluorogenic HDAC4 Assay Kit (BPS Bioscience, USA).

    Article Title: Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue
    Article Snippet: The statistical data analysis and IC 50 values were determined using GraphPad Prism 7.02. .. HDAC4 inhibitory activity was determined using the fluorogenic HDAC4 assay kit (BPS Bioscience, catalogue # 50064) according to the manufacturer procedure. .. The statistical data analysis and IC 50 values were determined using GraphPad Prism 7.02.

    Article Title: Oxadiazoline compounds or salts thereof, agrohorticultural fungicides containing the compounds, and methods of using the same
    Article Snippet: .. The fluorogenic HDAC4 assay kit (BPS Bioscience, Cat No. 50064) was used for HDAC4 inhibitory activity measurement. .. The fluorogenic HDAC4 assay kit (BPS Bioscience, Cat No. 50064) was used for HDAC4 inhibitory activity measurement.

    Lysis:

    Article Title: Curcumin attenuates pressure overload-induced cardiac fibrosis in mice via modulating the miR-29b/HDAC4 axis
    Article Snippet: HDAC4 activity was measured using the Fluorogenic HDAC4 Assay Kit (BPS Bioscience, #50064) following the manufacturer’s protocol. .. Tissue lysates were prepared from left ventricular tissue using the lysis buffer provided in the Fluorogenic HDAC4 Assay Kit (BPS Bioscience, USA). ..



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    BPS Bioscience hdac4 fluorogenic assay kit
    ( A ) Antennal and whole-mount brain staining of Gr63a-Gal4 flies. Flies were exposed to diacetyl (headspace from 10 –2 soln) or air for 2–5 d. Brains and antenna were dissected on the indicated days, fixed, and then stained for neuropil marker nc82 (red) and anti-GFP (green). Magnification 25 x. ( B ) Mean of ab1C neurons expressing GFP after indicated days of odor exposure. d4on = 2,3-butanedione. n = 6, error bars = SEM. Schematic chemical structures of diacetyl, β-hydroxybutyrate, and sodium butyrate. ( C ) Dose–activity curves of class I HDACs: HDAC1, HDAC2, HDAC3, HDAC8, and class II HDAC6 treated with various concentrations of diacetyl. Percentage of HDAC activity is relative to the activity of each enzyme without diacetyl. IC50s are indicated in the chart areas. Error bars = SEM, n = 4–5. ( D ) Representative structures of odorants that inhibit HDACs (left), and average percentage inhibition of class I HDACs: HDAC1, HDAC3, and class II <t>HDAC4,</t> HDAC6 treated with 15 mM of indicated volatiles (right). Error bar = SD, each tested in duplicate.
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    Image Search Results


    ( A ) Antennal and whole-mount brain staining of Gr63a-Gal4 flies. Flies were exposed to diacetyl (headspace from 10 –2 soln) or air for 2–5 d. Brains and antenna were dissected on the indicated days, fixed, and then stained for neuropil marker nc82 (red) and anti-GFP (green). Magnification 25 x. ( B ) Mean of ab1C neurons expressing GFP after indicated days of odor exposure. d4on = 2,3-butanedione. n = 6, error bars = SEM. Schematic chemical structures of diacetyl, β-hydroxybutyrate, and sodium butyrate. ( C ) Dose–activity curves of class I HDACs: HDAC1, HDAC2, HDAC3, HDAC8, and class II HDAC6 treated with various concentrations of diacetyl. Percentage of HDAC activity is relative to the activity of each enzyme without diacetyl. IC50s are indicated in the chart areas. Error bars = SEM, n = 4–5. ( D ) Representative structures of odorants that inhibit HDACs (left), and average percentage inhibition of class I HDACs: HDAC1, HDAC3, and class II HDAC4, HDAC6 treated with 15 mM of indicated volatiles (right). Error bar = SD, each tested in duplicate.

    Journal: eLife

    Article Title: Plasticity of gene expression in the nervous system by exposure to environmental odorants that inhibit HDACs

    doi: 10.7554/eLife.86823

    Figure Lengend Snippet: ( A ) Antennal and whole-mount brain staining of Gr63a-Gal4 flies. Flies were exposed to diacetyl (headspace from 10 –2 soln) or air for 2–5 d. Brains and antenna were dissected on the indicated days, fixed, and then stained for neuropil marker nc82 (red) and anti-GFP (green). Magnification 25 x. ( B ) Mean of ab1C neurons expressing GFP after indicated days of odor exposure. d4on = 2,3-butanedione. n = 6, error bars = SEM. Schematic chemical structures of diacetyl, β-hydroxybutyrate, and sodium butyrate. ( C ) Dose–activity curves of class I HDACs: HDAC1, HDAC2, HDAC3, HDAC8, and class II HDAC6 treated with various concentrations of diacetyl. Percentage of HDAC activity is relative to the activity of each enzyme without diacetyl. IC50s are indicated in the chart areas. Error bars = SEM, n = 4–5. ( D ) Representative structures of odorants that inhibit HDACs (left), and average percentage inhibition of class I HDACs: HDAC1, HDAC3, and class II HDAC4, HDAC6 treated with 15 mM of indicated volatiles (right). Error bar = SD, each tested in duplicate.

    Article Snippet: Commercial assay or kit , HDAC4 Fluorogenic Assay Kit , BPS Bioscience , 50064 , .

    Techniques: Staining, Marker, Expressing, Activity Assay, Inhibition

    Journal: eLife

    Article Title: Plasticity of gene expression in the nervous system by exposure to environmental odorants that inhibit HDACs

    doi: 10.7554/eLife.86823

    Figure Lengend Snippet:

    Article Snippet: Commercial assay or kit , HDAC4 Fluorogenic Assay Kit , BPS Bioscience , 50064 , .

    Techniques: Sequencing, Activity Assay, Isolation, SYBR Green Assay, Protease Inhibitor, Western Blot